Affiliations 

  • 1 Advanced Medical & Dental Institute, Universiti Sains Malaysia, Kepala Batas, Penang, Malaysia
  • 2 Advanced Medical & Dental Institute, Universiti Sains Malaysia, Kepala Batas, Penang, Malaysia; Institute of Nano Electronic Engineering & School of Bioprocess Engineering, Universiti Malaysia Perlis, Kangar, Perlis, Malaysia
  • 3 Department of Biotechnology, Faculty of Applied Sciences AIMST University, Bedong, Malaysia
  • 4 Institute of Experimental Pathology, University of Muenster, Muenster, Germany
PLoS One, 2015;10(3):e0118668.
PMID: 25774907 DOI: 10.1371/journal.pone.0118668

Abstract

Salmonellosis, a communicable disease caused by members of the Salmonella species, transmitted to humans through contaminated food or water. It is of paramount importance, to generate accurate detection methods for discriminating the various Salmonella species that cause severe infection in humans, including S. Typhi and S. Paratyphi A. Here, we formulated a strategy of detection and differentiation of salmonellosis by a multiplex polymerase chain reaction assay using S. Typhi non-protein coding RNA (sRNA) genes. With the designed sequences that specifically detect sRNA genes from S. Typhi and S. Paratyphi A, a detection limit of up to 10 pg was achieved. Moreover, in a stool-seeding experiment with S. Typhi and S. Paratyphi A, we have attained a respective detection limit of 15 and 1.5 CFU/mL. The designed strategy using sRNA genes shown here is comparatively sensitive and specific, suitable for clinical diagnosis and disease surveillance, and sRNAs represent an excellent molecular target for infectious disease.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.