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Peptide Cross-Linked Poly (Ethylene Glycol) Hydrogel Films as Biosensor Coatings for the Detection of Collagenase

Ahmad N 1 , Colak B 2 , Zhang DW 3 , Gibbs MJ 4 , Watkinson M 5 , Becer CR 6 Show all authors , Gautrot JE 7 , Krause S 8

Affiliations 

  • 1 School of Engineering and Material Science, Queen Mary University of London, Mile End Road, London, E1 4NS, UK. norlaily.ahmad@qmul.ac.uk
  • 2 School of Engineering and Material Science, Queen Mary University of London, Mile End Road, London, E1 4NS, UK. b.colak@qmul.ac.uk
  • 3 Institute of Medical Engineering, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, Xi'an 710061, China. zhangdewen@xjtu.edu.cn
  • 4 School of Engineering and Material Science, Queen Mary University of London, Mile End Road, London, E1 4NS, UK. m.j.gibbs@qmul.ac.uk
  • 5 The Lennard-Jones Laboratories, School of Chemical and Physical Sciences, Keele University, Staffordshire, ST5 5BG, UK. m.watkinson@keele.ac.uk
  • 6 Department of Chemistry, University of Warwick, Coventry, CV4 7AL, UK. remzi.becer@warwick.ac.uk
  • 7 School of Engineering and Material Science, Queen Mary University of London, Mile End Road, London, E1 4NS, UK. j.gautrot@qmul.ac.uk
  • 8 School of Engineering and Material Science, Queen Mary University of London, Mile End Road, London, E1 4NS, UK. s.krause@qmul.ac.uk
Sensors (Basel), 2019 Apr 08;19(7).
PMID: 30965649 DOI: 10.3390/s19071677

Abstract

Peptide cross-linked poly(ethylene glycol) hydrogel has been widely used for drug delivery and tissue engineering. However, the use of this material as a biosensor for the detection of collagenase has not been explored. Proteases play a key role in the pathology of diseases such as rheumatoid arthritis and osteoarthritis. The detection of this class of enzyme using the degradable hydrogel film format is promising as a point-of-care device for disease monitoring. In this study, a protease biosensor was developed based on the degradation of a peptide cross-linked poly(ethylene glycol) hydrogel film and demonstrated for the detection of collagenase. The hydrogel was deposited on gold-coated quartz crystals, and their degradation in the presence of collagenase was monitored using a quartz crystal microbalance (QCM). The biosensor was shown to respond to concentrations between 2 and 2000 nM in less than 10 min with a lower detection limit of 2 nM.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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