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Development and characterization of microsatellite markers in sawih tree (Duabanga moluccana Blume) using ISSR-suppression PCR techniques

Liew KS 1 , Ho WS 1 , Pang SL 2 , Julaihi A 2

Affiliations 

  • 1 Forest Genomics and Informatics Laboratory (fGiL), Department of Molecular Biology, Faculty of Resource Science & Technology, Universiti Malaysia Sarawak, 94300 Kota Samarahan, Sarawak Malaysia
  • 2 Applied Forest Science and Industry Development (AFSID), Sarawak Forestry Corporation, 93250 Kuching, Sarawak Malaysia
Physiol Mol Biol Plants, 2015 Jan;21(1):163-5.
PMID: 25649417 DOI: 10.1007/s12298-014-0262-2

Abstract

Duabanga moluccana (or locally known as sawih) is an indigenous fast growing tropical tree species that confers various advantages for the timber industry and for planted forests development. In this paper, we isolated and characterized 8 polymorphic microsatellite markers from the D. moluccana genome using ISSR-suppression PCR techniques. The number of alleles and PIC values ranged from 3 to 8 alleles per locus and from 0.488 to 0.792, respectively. Three microsatellite loci were deviated from Hardy-Weinberg equilibrium (P 

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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