The objective of the present preliminary study was to investigate the effect of platelet-rich plasma (PRP) treatment
alongside rehabilitation programme compared with rehabilitation programme alone on antioxidant enzymes’ (superoxide
dismutase, SOD; catalase, CAT) activity and time to return to play (RTP) following hamstring injury among Malaysian
athletes. Participants diagnosed with grade-2 acute hamstring injury (n=10) were randomised into 2 groups of PRP
treatment and rehabilitation programme (PRP-T) and rehabilitation programme alone (CON). Blood samples were collected
at baseline and 2 fortnightly (week-2, W2; week-4, W4) for the biochemical assessments. Participants were certified to
have recovered upon fulfilling RTP criteria. PRP-T group showed a significantly lower CAT activity compared to CON group
(47.55% lower at W2, 37% lower at W4, p<0.05), while there was no significant difference in SOD activity between the
groups. Although PRP-T group benefited from an earlier (~15 days) time to RTP compared to the CON group, no statistically
significant difference was noted; however, the effect size was large (Cohen’s d=0.9). These findings suggested that PRP
treatment alongside rehabilitation potentially reduces the degree of secondary tissue damage and facilitates muscle
recovery through regulation of antioxidant enzymes, which subsequently hastens time to RTP. Athletes and coaches could
consider PRP as viable treatment for hamstring injury.