Increasing demands for stingless bee honey in Malaysia have prompted alternative method using plastic cups as artificial
honey pots within beehives to enhance yield. However, honey’s acidity may lead to migration of plastic additives, affecting
body’s physiological responses on prolonged consumption. This study was performed to determine the presence of dibutyl
phthalate (DBP) and oleamide in stingless bee honey collected from plastic cups. A method for simultaneous extraction
and detection of both analytes by solvent terminated dispersive liquid-liquid microextraction (ST-DLLME) coupled with high
performance liquid chromatography (HPLC) was developed. Good linearity was observed with coefficient of determination
R2
≥ 0.97 in the concentration range of 0.5-50 and 10-250 µg/g for DBP and oleamide, respectively. The limit of detection
for DBP was 0.15 µg/g; while oleamide was 4 µg/g. The limit of quantitation for DBP and oleamide was 0.5 and 11 µg/g,
respectively. DBP and oleamide were not detected in all the honey samples collected.