Graphene-magnetite as adsorbent for magnetic solid phase extraction of 4-hydroxybenzoic acid and 3,4-dihydroxybenzoic acid in stingless bee honey

Musa M 1 , Wan Ibrahim WA 2 , Mohd Marsin F 1 , Abdul Keyon AS 1 , Rashidi Nodeh H 3

Affiliations 

  • 1 Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia, 81310 UTM, Johor Bahru, Johor, Malaysia
  • 2 Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia, 81310 UTM, Johor Bahru, Johor, Malaysia; Centre for Sustainable Nanomaterials, Ibnu Sina Institute for Scientific and Industrial Research, Universiti Teknologi Malaysia, 81310 UTM, Johor Bahru, Johor, Malaysia. Electronic address: waini@utm.my
  • 3 Young Researchers and Elite Club, Science and Research Branch, Islamic Azad University, Tehran, Iran
Food Chem, 2018 Nov 01;265:165-172.
PMID: 29884368 DOI: 10.1016/j.foodchem.2018.04.020

Abstract

Graphene-magnetite composite (G-Fe3O4) was successfully synthesized and applied as adsorbent for magnetic solid phase extraction (MSPE) of two phenolic acids namely 4-hydroxybenzoic acid (4-HB) and 3,4-dihydroxybenzoic acid (3,4-DHB) from stingless bee honey prior to analysis using high performance liquid chromatography with ultraviolet-visible detection (HPLC-UV/Vis). Several MSPE parameters affecting extraction of these two acids were optimized. Optimum MSPE conditions were 50 mg of G-Fe3O4 adsorbent, 5 min extraction time at 1600 rpm, 30 mL sample volume, sample solution pH 0.5, 200 µL methanol as desorption solvent (5 min sonication assisted) and 5% w/v NaCl. The LODs (3 S/N) calculated for 4-HB and 3,4-DHB were 0.08 and 0.14 µg/g, respectively. Good relative recoveries (72.6-110.6%) and reproducibility values (RSD 

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