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Genetic diversity of Plasmodium knowlesi among human and long-tailed macaque populations in Peninsular Malaysia: The utility of microsatellite markers

Saleh Huddin A 1 , Md Yusuf N 2 , Razak MRMA 3 , Ogu Salim N 1 , Hisam S 1

Affiliations 

  • 1 Parasitology Unit, Infectious Disease Research Centre, Institute for Medical Research, Jalan Pahang, 50588 Kuala Lumpur, Malaysia
  • 2 Parasitology Unit, Infectious Disease Research Centre, Institute for Medical Research, Jalan Pahang, 50588 Kuala Lumpur, Malaysia. Electronic address: noorazian@imr.gov.my
  • 3 Bioassay Unit, Herbal Medicine Research Centre, Jalan Pahang, 50588 Kuala Lumpur, Malaysia
Infect Genet Evol, 2019 11;75:103952.
PMID: 31279818 DOI: 10.1016/j.meegid.2019.103952

Abstract

It has been discovered that Plasmodium knowlesi (P. knowlesi) is transmitted from macaque to man. Thus, the aim of the present study was to determine P. knowlesi genetic diversity in both human (n = 147) and long-tailed macaque (n = 26) samples from high- and low-endemicity localities. Genotyping was performed using seven neutral microsatellite loci markers. The size of the alleles, multiplicity of infection (MOI), mean number of alleles (Na), expected heterozygosity (HE), linkage disequilibrium (LD), and genetic differentiation (FST) were determined. In highly endemic P. knowlesi localities, the MOI for human and long-tailed macaque isolates was 1.04 and 1.15, respectively, while the Na was 11.14 and 7.86, respectively. Based on the allele frequency distribution for all loci, and with FST 

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