Affiliations 

  • 1 College of Natural Sciences, Department of Biological Sciences, Kongju National University, Gongju, 32588, South Korea
  • 2 Department of Chemistry, Government College University, Lahore, 54000, Pakistan
  • 3 Institute of Molecular Biology and Biotechnology, The University of Lahore, Pakistan
  • 4 Faculty of Pharmacy and Atta-ur-Rahman Institute for Natural Products Discovery (AuRIns), Universiti Teknologi MARA, Cawangan Selangor Kampus, Puncak Alam, 42300 Bandar Puncak Alam, Selangor Darul Ehsan, Malaysia
Toxicol Rep, 2019;6:897-903.
PMID: 31516842 DOI: 10.1016/j.toxrep.2019.08.016

Abstract

In the study presented here, a novel chlorobenzylated bi-heterocyclic hybrid molecule (7) was synthesized and its structural confirmation was carried out by IR, 1H-NMR, 13C-NMR and CHN analysis data. This compound 7 was subjected to biological study with B16F10 mouse melanoma cells. The anti-proliferative results showed that 7 showed no significant toxicity at concentrations ranging of 0-44 μM. The treatment of B16F10 cells with 7 at aforementioned concentration range indicated that migration of cells was significantly lower than that of the control cells in a dose dependent manner. The possible migration inhibitory effect of these melanoma cells was further evaluated through gelatinolytic activity of MMP-2 and MMP-9 secreted from B16F10 cells. It was inferred from our results that 7 was not affecting the expression and activity of these enzymes. Some other zinc-dependent matrix metalloproteinases (MMPs) were involved in the inhibitory progression. Taken together, compound 7 inhibited migrations of B16F10 mouse melanoma cells. Therefore, it may deserve consideration as a potential agent for the treatment of cancer.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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