Affiliations 

  • 1 Wuya College of Innovation, Shenyang Pharmaceutical University, Shenyang, Liaoning 110013, China; Faculty of Pharmacy, AIMST University, Semeling, 08100 Bedong, Kedah, Malaysia. Electronic address: hriday.bera1@gmail.com
  • 2 Faculty of Pharmacy, AIMST University, Semeling, 08100 Bedong, Kedah, Malaysia
  • 3 Agharkar Research Institute, Pune, Maharashtra 411004, India
  • 4 School of Pharmacy, University of Reading Malaysia, Educity, 79200 Iskandar Puteri, Johor, Malaysia
  • 5 Faculty of Pharmacy, International Islamic University Malaysia, 25200 Indera Mahkota, Pahang, Malaysia
  • 6 Wuya College of Innovation, Shenyang Pharmaceutical University, Shenyang, Liaoning 110013, China
  • 7 Wuya College of Innovation, Shenyang Pharmaceutical University, Shenyang, Liaoning 110013, China; Department of Pharmacy, University of Copenhagen, Copenhagen, Denmark
Mater Sci Eng C Mater Biol Appl, 2020 May;110:110628.
PMID: 32204068 DOI: 10.1016/j.msec.2020.110628

Abstract

The current study dealt with the synthesis and characterization of carboxymethyl fenugreek galactomannang-g-poly(N-isopropylacrylamide-co-N,N'-methylene-bis-acrylamide)-bentonite [CFG-g-P(NIPA-co-MBA)-BEN] based nanocomposites (NCs) as erlotinib (ERL)-delivery devices for lung cancer cells to suppress excessive cell proliferation. The blank NCs exhibited outstanding biodegradability and pH/temperature-dependent swelling profiles, which were significantly influenced by their BEN contents (0-20%). The molar mass (M¯c) between the crosslinks of these NCs was declined with temperature. The composite architecture of these scaffolds was confirmed by XRD, FTIR, TGA, DSC and SEM analyses. The corresponding ERL-loaded matrices (F-1-F-3) portrayed outstanding drug encapsulation efficiency (DEE, 93-100%) with zeta potential between -8 and -16 mV and diameter between 615 and 1258 nm. These formulations demonstrated sustained ERL elution profiles (Q8h, 62-98%) with an initial burst release of drug. The drug dissolution pattern of the optimized matrices (F-3) obeyed first-order kinetic model and was driven by Fickian diffusion. The mucin adsorption behavior of F-3 was best fitted to Freudlich isotherms. The ERL-loaded formulation suppressed A549 cell proliferation and promoted apoptosis to a greater extent than the pristine drug, as detected by cellular uptake analysis, MTT cytotoxicity test and AO/EB staining assay.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.