Affiliations 

  • 1 Department of Botany and Microbiology, College of Sciences, King Saud University, P.O. Box 22452, Riyadh, 11495, Saudi Arabia
  • 2 Department of Environmental Engineering, Faculty of Engineering and Science, Curtin University, Malaysia, CDT 250, 98009, Miri, Sarawak, Malaysia. hadibarata@curtin.edu.my
  • 3 Department of Environmental Engineering, Faculty of Civil, Planning, and Geo-Engineering, Institut Teknologi Sepuluh Nopember, Surabaya, 60111, Indonesia
  • 4 Department of Public Health, Faculty of Health, Universitas Nahdlatul Ulama Surabaya, Surabaya, East Java, 60237, Indonesia
Bioprocess Biosyst Eng, 2020 Dec;43(12):2305-2314.
PMID: 32812060 DOI: 10.1007/s00449-020-02415-4

Abstract

The present study aimed to determine the degradation and transformation of three-ring PAHs phenanthrene and anthracene by Cryptococcus sp. MR22 and Halomonas sp. BR04 under halophilic conditions. The growth progress of Cryptococcus sp. MR22 and Halomonas sp. BR04 on anthracene and phenanthrene was monitored by colony-forming unit (CFU) technique. The growth of the bacteria was maintained at a maximum concentration of 200 mg/L of all tested hydrocarbon, indicating that Cryptococcus sp. MR22 and Halomonas sp. BR04 significantly perform in the removal of the PAH-contaminated medium at low concentrations. The fit model to represent the biodegradation kinetics of both PAHs was first-order rate equation The extract prepared from cells supplemented with three different substrates exhibited some enzymes such as hydroxylase, dioxygenase, laccase and peroxidase. The results suggest that both strains had an impressive ability in the degradation of aromatic and aliphatic hydrocarbon but also could tolerate in the extreme salinity condition.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.