Affiliations 

  • 1 Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori Amamiyamachi, Aoba-ku, Sendai, Miyagi 981-8555, Japan
  • 2 Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori Amamiyamachi, Aoba-ku, Sendai, Miyagi 981-8555, Japan ; Molecular Population Genetics Group, Temasek Lifesciences Laboratory, 1 Research Link, National University of Singapore, Singapore 117604
  • 3 ACGT Sdn. Bhd. Lot L3-I-1, Enterprise 4, Technology Park Malaysia, 57000 Kuala Lumpur, Malaysia
  • 4 ACGT Sdn. Bhd. Lot L3-I-1, Enterprise 4, Technology Park Malaysia, 57000 Kuala Lumpur, Malaysia ; National Heart Centre Singapore Pte. Ltd., 17 Third Hospital Avenue No. 01-00, Singapore 168752
Biomed Res Int, 2014;2014:467395.
PMID: 25177691 DOI: 10.1155/2014/467395

Abstract

Amphidiploid species in the Brassicaceae family, such as Brassica napus, are more tolerant to environmental stress than their diploid ancestors.A relatively salt tolerant B. napus line, N119, identified in our previous study, was used. N119 maintained lower Na(+) content, and Na(+)/K(+) and Na(+)/Ca(2+) ratios in the leaves than a susceptible line. The transcriptome profiles of both the leaves and the roots 1 h and 12 h after stress were investigated. De novo assembly of individual transcriptome followed by sequence clustering yielded 161,537 nonredundant sequences. A total of 14,719 transcripts were differentially expressed in either organs at either time points. GO and KO enrichment analyses indicated that the same 49 GO terms and seven KO terms were, respectively, overrepresented in upregulated transcripts in both organs at 1 h after stress. Certain overrepresented GO term of genes upregulated at 1 h after stress in the leaves became overrepresented in genes downregulated at 12 h. A total of 582 transcription factors and 438 transporter genes were differentially regulated in both organs in response to salt shock. The transcriptome depicting gene network in the leaves and the roots regulated by salt shock provides valuable information on salt resistance genes for future application to crop improvement.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.