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Mitochondrial DNA sequence of the horseshoe crab Tachypleus gigas

Sarmiento ME 1 , Chin KL 2 , Lau NS 3 , Aziah I 4 , Norazmi MN 1 , Acosta A 1 Show all authors , Ismail N 5 , Yaacob NS 6

Affiliations 

  • 1 School of Health Sciences, Universiti Sains Malaysia, Health Campus, Kelantan, Malaysia
  • 2 Faculty of Medicine and Health Sciences, Universiti Malaysia Sabah, Kota Kinabalu, Malaysia
  • 3 Centre for Chemical Biology, Universiti Sains Malaysia, Pulau Pinang, Malaysia
  • 4 Institute for Research in Molecular Medicine, Universiti Sains Malaysia, Health Campus, Kelantan, Malaysia
  • 5 Institute of Marine Biotechnology, Universiti Malaysia Terengganu, Terengganu, Malaysia
  • 6 Department of Chemical Pathology, School of Medical Sciences, Universiti Sains Malaysia, Health Campus, Kelantan, Malaysia
Mitochondrial DNA B Resour, 2021 May 23;6(6):1710-1714.
PMID: 34104748 DOI: 10.1080/23802359.2021.1930213

Abstract

This paper reports on the complete mitochondrial (mt) genome of a horseshoe crab, Tachypleus gigas (T. gigas), in Kuala Kemaman, Terengganu, Malaysia. Whole-genome sequencing of hemocyte DNA was performed with Illumina HiSeq system and the generated reads were de novo assembled with ABySS 2.1.5 and reassembled using mitoZ against Carcinoscorpius rotundicauda and Limulus polyphemus, resulting in a contig of 15 Kb. Phylogenetic analysis of the assembled mt genome suggests that the Tachypleus gigas is closely related to Tachypleus tridentatus than to Carcinoscorpius rotundicauda.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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