Comparative transcriptome profiling of horseshoe crab Tachypleus gigas hemocytes in response to lipopolysaccharides

Sarmiento ME 1 , Chin KL 2 , Lau NS 3 , Aziah I 4 , Ismail N 5 , Norazmi MN 1 Show all authors , Acosta A 1 , Yaacob NS 6

Affiliations 

  • 1 School of Health Sciences, Universiti Sains Malaysia, Health Campus, Kubang Kerian, Kelantan, Malaysia
  • 2 Faculty of Medicine and Health Sciences, Universiti Malaysia Sabah, Kota Kinabalu, Sabah, Malaysia
  • 3 Centre for Chemical Biology, Universiti Sains Malaysia, Bayan Lepas, Pulau Pinang, Malaysia
  • 4 Institute for Research in Molecular Medicine, Universiti Sains Malaysia, Health Campus, Kubang Kerian, Kelantan, Malaysia
  • 5 Institute of Marine Biotechnology, Universiti Malaysia Terengganu, Kuala Nerus, Terengganu, Malaysia
  • 6 Department of Chemical Pathology, School of Medical Sciences, Universiti Sains Malaysia, Health Campus, Kubang Kerian, Kelantan, Malaysia. Electronic address: niksoriani@usm.my
Fish Shellfish Immunol, 2021 Oct;117:148-156.
PMID: 34358702 DOI: 10.1016/j.fsi.2021.08.001

Abstract

Horseshoe crabs (HSCs) are living fossil species of marine arthropods with a long evolutionary history spanning approximately 500 million years. Their survival is helped by their innate immune system that comprises cellular and humoral immune components to protect them against invading pathogens. To help understand the genetic mechanisms involved, the present study utilised the Illumina HiSeq platform to perform transcriptomic analysis of hemocytes from the HSC, Tachypleus gigas, that were challenged with lipopolysaccharides (LPS). The high-throughput sequencing resulted in 352,077,208 and 386,749,136 raw reads corresponding to 282,490,910 and 305,709,830 high-quality mappable reads for the control and LPS-treated hemocyte samples, respectively. Based on the log-fold change of > 0.3 or 

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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