Affiliations 

  • 1 School of Science, Monash University Malaysia, Bandar Sunway, Malaysia
  • 2 School of Science, Monash University Malaysia, Bandar Sunway, Malaysia. Electronic address: kumaran.narayanan@monash.edu
Anal Biochem, 2021 10 01;630:114324.
PMID: 34363787 DOI: 10.1016/j.ab.2021.114324

Abstract

The effect of DNA topology on transfection efficiency of mammalian cells has been widely tested on plasmids smaller than 10 kb, but little is known for larger DNA vectors carrying intact genomic DNA containing introns, exons, and regulatory regions. Here, we demonstrate that circular BACs transfect more efficiently than covalently closed linear BACs. We found up to 3.1- and 8.9- fold higher eGFP expression from circular 11 kb and 100 kb BACs, respectively, compared to linear BACs. These findings provide insights for improved vector development for gene delivery and expression studies of large intact transgenes in mammalian cells.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.