MyMedR

Displaying all 8 publications

Abstract:

Sort:

Fulltext Pengimejan dari Mikroskop Imbasan (SEM) terhadap Kesan Antibakteria Ekstrak Etilingera elatior (Bunga Kantan)

HING HIANG LIAN, AHMAD ZORIN SAHALAN

Buletin Sains Kesihatan, 2021;5(1):43-46.
MyJurnal

Elektron mikroskop digunakan untuk melihat tindakbalas bahan ekstrak air kantan atau Etilingera elatior akan mekanisma tindakbalasnya. Di dalam kajian ini kesan tindakbalas antibakteria bunga kantan telah di kaji. Pengimejan dari elektron mikroskop imbasan telah memberikan imej kerosakan pada permukaan bakteria yang disebabkan oleh ekstrak air kantan. Ekstrak kantan pada kepekatan 1.5mg/ml, merencat pertumbuhan bakteria S. aureus dan B.subtilis . Apa yang penting adalah kesan mekanisma tindakbalas ekstrak kantan yang menghasilkan kerosakan seperti lekukan pada bakteria Gram positif. Ini menunjukkan ekstrak air kantan sangat berkesan terhadap lapisan peptidoglikan pada permukaan Gram positif sehingga kerosakan ini menyebabkan kesan bakterisidal.
Fulltext Special Issue - 5th International Anatomical Sciences & Cell Biology Conference

B Hemabarathy Bharatham, Siti Fathiah Masre, Hing Hiang Lian, Baharudin Omar

Life Sciences, Medicine and Biomedicine, 2019;3(3):0-0.
MyJurnal

Special Issue - 5th International Anatomical Sciences & Cell Biology Conference
Divalent cations (Mg2+, Ca2+) protect bacterial outer membrane damage by polymyxin B

Ahmad Zorin Sahalan, Abdulhamid Abd. Aziz, Hing Hiang Lian, Mohamed Kamel Abd. Ghani

Sains Malaysiana, 2013;42:301-306.

Polymyxin B interacts with divalent cations by displacing cations from their binding sites in the lipopolysaccharide (LPS) molecules. It leads to the disorganization of the outer membrane component of the Gram negative bacteria, which releases LPS component from bacterial surface causing severe membrane leakage and finally cell death. In this paper, the reversible activity of PMB was investigated in the presence of access divalent cations such as Mg2+ or Ca2+. Membrane damage and cell disruption were monitored by detecting leakage of the outer membrane and cytoplasmic enzyme marker (β-lactamase and β-galactosidase), release of LPS component (KDO) from bacterial cells and bacterial survival. With the presence of divalent cations, leakage of enzyme markers and LPS released was significantly reduced when bacteria were exposed to PMB. The survival curve also significantly increased. The inhibitory of damage caused by PMB also depended on the type of divalent cation present. Ca2+ has been shown to be more effective in protecting the
bacteria cells than Mg2+. This is because Ca2+ appears more frequently as a constituent of the structural components of the bacteria. In conclusion, the presence of higher concentration of divalent cation particularly with Ca2+ inhibits PMB activity and maintained bacterial survival.
Decomposition process and post mortem changes: review

Teo Chee Hau, Noor Hazfalinda Hamzah, Hing Hiang Lian, Sri Pawita Albakri Amir Hamzah

Sains Malaysiana, 2014;43:1873-1882.

Decomposition is degradation process of a corpse into basic respective constituents macroscopically and microscopically by action of microorganisms, arthropods and scavengers. Post mortem changes could be separated into early post mortem changes (i.e. algor mortis, rigor mortis and livor mortis) and putrefaction stages of corpse. These changes function as suitable indicators for determination of post mortem interval (PMI). In this paper, different stages of post mortem changes, possible variations such as mummification and formation of adipocere and special circumstances such as burial condition is discussed. This article also refers to several arguments in the different texture of adipocere and the influence of different types of fabric in affecting the post mortem changes and formation of adipocere. This is largely due to the property of permeability and resistance of material against degradation process. Undeniably, decomposition process involves numerous potential variables including burial condition, presence of clothing, potential formation of adipocere and mummification. Hence, studies in forensic taphonomy combined with real case scenario are crucial in understanding the nature of decomposition and estimation of PMI with higher accuracy.
Fulltext Pengenalpastian mikroflora bakteria gram positif dan negatif yang dipencil daripada kolam air panas Bentong, Pahang

Abdul Hamid Abdul Aziz, Mohamed Kamel Abd Ghani, Ahmad Zorin Sahalan, Hing, Hiang Lian

Jurnal Sains Kesihatan Malaysia, 2018;16(2):1-4.
MyJurnal

Suatu kajian pemencilan dan pengenalpastian mikroflora bakteria daripada kolam air panas telah dilakukan di kolam
air panas Bentong, Pahang. Kebanyakan bakteria yang dipencil dari kolam air panas adalah dari Bacillus sp. kerana ia
mempunyai ciri adaptasi yang membolehkannya hidup di kawasan ekstrem suhu tinggi dengan pembentukan endospora.
Objektif kajian adalah untuk mengenal pasti spesies bakteria selain dari spesies Bacillus sp. yang mampu mandiri dalam
kolam air panas ini. Tiga buah kolam air panas dikenal pasti sebagai A, B dan C. Julat suhu berada antara 36°C-52°C
dan nilai pH antara 7.88-8.65. Kajian ini telah berjaya memencilkan dua isolat bakteria kokus Gram positif bersama
tiga spesies Gram negatif. Spesies Gram positif tersebut adalah dari spesies Streptococcus sp. dan Staphylococcus sp,
manakala Gram negatif pula dikenal pasti sebagai Pseudomonas sp., Klebsiella sp. dan Proteus sp.. Sebagai kesimpulan,
bakteria tak berspora yang bukan Bacillus sp. mampu mandiri dalam kolam air panas.
Fulltext Pengenalpastian mikroflora bakteria selain bacillus sp. Dari kolam air panas Bentong, Pahang, Malaysia

Abdul Hamid Abdul Aziz, Nur Fariza Mat Reffin, Mohamed Kamel Abd Ghani, Hing, Hiang Lian, Ahmad Zorin Sahalan

Jurnal Sains Kesihatan Malaysia, 2012;10(2):53-55.
MyJurnal

Suatu kajian mengenai pemencilan dan pengenalpastian mikroflora bakteria dari kolam air panas telah dilakukan di kolam air panas Bentong, Pahang. Kebanyakan bakteria yang dipencil dari kolam air panas adalah dari Bacillus sp. kerana ia mempunyai ciri adaptasi yang membolehkannya hidup di kawasan suhu yang tinggi dengan pembentukan endospora. Objektif kajian adalah untuk mengenal pasti spesies bakteria selain daripada spesies Bacillus sp. yang boleh dipencilkan dari kolam air panas. Tiga buah kolam air panas (A, B dan C) dikenal pasti dengan julat suhunya antara 36°C-52°C dan nilai pH antara 7.88-8.65. Kajian ini telah berjaya memencilkan dua pencilan bakteria kokus Gram positif dan tiga spesies basilus Gram negatif. Spesies Gram positif tersebut adalah dari genus Streptococcus dan Staphylococcus, manakala bagi Gram negatif pula terdapat masalah dalam pengenalpastian dan hanya dikenali sebagai X, XI and XII.
Fulltext Short note protective effect of citrullus vulgaris on irradiated lymphocyte membrane ultrastructure

Khairul Osman, Norashikin, M.S., Hing, Hiang Lian, Siti Fatimah Ibrahim, Seetha Khartini Abdul Wahab, Jamaludin Mohamed, et al.

Journal of Nuclear and Related Technologies, 2004;2004(2):75-79.
MyJurnal

Radiotherapy causes various complications including low immunity. Past research has shown that the low immunity is due to the low amount of lymphocytes and consumption of citrullus vulgaris will alleviate this problem. Based on this a study was conducted to identify how citrullus vulgaris was able to produce radioprotection on the lymphocyte membrane. A total of 30 adult male Sprague-Dawley rats were used and divided into three equals groups of positive control, negative control and treatment. For seven days, positive control and negative control were force fed with normal saline of 40 ml/kg animal weight while the treatment group received 40 g/kg animal weight fresh juice of citrullus vulgaris daily. After a week positive control and treatment group were irradiated with 0.9 Gy gamma ray. Viable lymphocyte were determined using propidium iodine and acridine orange stain. Results clearly shows that positive control, negative and treatment group were significantly different at 34  3% , 80  2% and 71  2% respectively. SEM results shows that pores were present on the membrane of the positive control while the negative control had none. Similar results were also found on the treatment group. Based on the result it had shown that citrullus vulgaris had radioprotection properties and lymphocytes were destroyed by the formation of pores on their membrane. It is very likely that the radioprotection properties could be due to the presence of antioxidants particularly vitamin A, C and lycopene. In conclusion, citrullus vulgaris could be used as a safe radioprotection agent.
Fulltext The Effect of Different Coverings on Total Body Score Development of Buried Carcasses

Teo CH, Hing HL, Hamzah NH, Hamzah SPAA

Malays J Med Sci, 2021 Aug;28(4):103-112.
PMID: 34512135 DOI: 10.21315/mjms2021.28.4.11

Background: Examination of post-mortem changes is one of the ways to assess decomposition process on buried bodies. Nonetheless, studies on such assessment are still lacking, especially on the effects of body coverings by different materials in burial conditions. The aim of this research is to study the effect of different types of covering towards decomposition on buried rabbit carcasses by using Total Body Score (TBS) system.
Methods: Twenty-seven rabbit carcasses were divided into: (i) No Clothing; (ii) Thick Clothing and (iii) Plastic Wrapping groups, and buried into individual shallow graves. One subject was exhumed from first to ninth post-burial week and assessed by using TBS system.
Results and Discussion: There are significant differences among TBS between different coverings while controlling the time factor, F (2, 23) = 4.80, P < 0.05, partial η 2 = 0.294. TBS score for Plastic Wrapping group is significantly lower than No Clothing group over times at α = 0.05, P < 0.05. The slightly delaying effect of thick clothing is caused by design of jacket, which allows arthropods access and microbial activity. Relatively strong delaying effect by plastic covering could be caused by impermeable property that caused accumulation of decompositional products and slow down the degradation.
Conclusion: In conclusion, TBS system is a potential tool in describing rate of decomposition for buried cases in Malaysia.