Displaying publications 1 - 20 of 41 in total

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  1. Hasan MR, Pulingam T, Appaturi JN, Zifruddin AN, Teh SJ, Lim TW, et al.
    Anal Biochem, 2018 08 01;554:34-43.
    PMID: 29870692 DOI: 10.1016/j.ab.2018.06.001
    In this study, an amino-modified aptasensor using multi-walled carbon nanotubes (MWCNTs)-deposited ITO electrode was prepared and evaluated for the detection of pathogenic Salmonella bacteria. An amino-modified aptamer (ssDNA) which binds selectively to whole-cell Salmonella was immobilised on the COOH-rich MWCNTs to produce the ssDNA/MWCNT/ITO electrode. The morphology of the MWCNT before and after interaction with the aptamers were observed using scanning electron microscopy (SEM). Cyclic voltammetry and electrochemical impedance spectroscopy techniques were used to investigate the electrochemical properties and conductivity of the aptasensor. The results showed that the impedance measured at the ssDNA/MWCNT/ITO electrode surface increased after exposure to Salmonella cells, which indicated successful binding of Salmonella on the aptamer-functionalised surface. The developed ssDNA/MWCNT/ITO aptasensor was stable and maintained linearity when the scan rate was increased from 10 mV s-1 to 90 mV s-1. The detection limit of the ssDNA/MWCNT/ITO aptasensor, determined from the sensitivity analysis, was found to be 5.5 × 101 cfu mL-1 and 6.7 × 101 cfu mL-1 for S. Enteritidis and S. Typhimurium, respectively. The specificity test demonstrated that Salmonella bound specifically to the ssDNA/MWCNT/ITO aptasensor surface, when compared with non-Salmonella spp. The prepared aptasensor was successfully applied for the detection of Salmonella in food samples.
  2. Mustafa NWNA, Ahmad R, Ahmad Khushaini MA, Kamar Affendi NH, Ab Ghani SM, Tan SK, et al.
    ACS Biomater Sci Eng, 2024 Jan 08;10(1):405-419.
    PMID: 38040671 DOI: 10.1021/acsbiomaterials.3c01551
    This study assessed the corrosion resistance, intracutaneous reactivity, acute systemic toxicity, and in situ tissue effect of the implantation of porous NiTi fabricated by metal injection molding in animal models. For the intracutaneous reactivity study, five intracutaneous injections were administered per site with and without the tested extract in polar and nonpolar solutions. The extract was also delivered via intravenous and intraperitoneal routes for acute systemic toxicity. TiAl6 V4 (control) and porous NiTi were implanted in rabbit femora for a period of 13 weeks to evaluate the in situ tissue response. Corrosion was evaluated through open and cyclic polarization in PBS, while biocompatibility was investigated by assessing the general conditions, skin irritation score (edema and erythema), and histopathology. No active dissolution or hysteresis loop was observed in the corrosion study. None of the animals exhibited death, moribundity, impending death, severe pain, self-mutilation, or overgrooming. No edema was observed at injection sites. Only the positive control showed an erythematous reaction at 24, 48, and 72 h observations (p < 0.001). Porous NiTi showed a low in situ biological response for inflammation, neovascularization, and fibrosis in comparison to the control implant (p = 0.247, 0.005, and 0.011, respectively). Porous NiTi also demonstrated high pitting corrosion resistance while causing no acute hypersensitivity or acute systemic toxicity. The study concludes that porous NiTi implants were unlikely to cause local sensitization, acute systemic toxicity, or chronic inflammatory reactions in an animal model. Porous NiTi also exhibited osseointegration equivalent to Ti6AI4 V of known biocompatibility.
  3. Ab Ghani SM, Mohd Khairuddin PNA, Lim TW, Md Sabri BA, Abdul Hamid NF, Baharuddin IH, et al.
    Eur J Dent Educ, 2024 Feb;28(1):106-113.
    PMID: 37253116 DOI: 10.1111/eje.12925
    INTRODUCTION: The communication skills of clinicians are very crucial in providing better health outcomes for patients. Therefore, this study aimed to assess undergraduate dental students' communication skills in relation to their demographics and clinical setting using a three-perspective approach; the student, the patient and the clinical instructor perspective.

    METHODS: A cross-sectional study was conducted using validated modified-communication tools; Patient Communication Assessment Instruments (PCAI), Student Communication Assessment Instruments (SCAI) and Clinical Communication Assessment Instruments (CCAI) which included four communication domains. One hundred and seventy-six undergraduate clinical year students were recruited in this study whereby each of them was assessed by a clinical instructor and a randomly selected patient in two settings: Dental Health Education (DHE) and Comprehensive Care (CC) clinic.

    RESULTS: Comparing the three perspectives, PCAI yielded the highest scores across all domains, followed by SCAI and CCAI (p 

  4. Lim TW, Ab Ghani SM, Mahmud M
    J Prosthet Dent, 2020 Dec 02.
    PMID: 33279159 DOI: 10.1016/j.prosdent.2020.06.034
    STATEMENT OF PROBLEM: Resin-bonded fixed partial dental prostheses (RBFPDPs) cemented at an increased occlusal vertical dimension (OVD) (the Dahl concept) to create space for a metal retainer remain controversial because of the lack of reported clinical studies. This study evaluated RBFPDPs by applying the Dahl concept.

    PURPOSE: The purpose of this retrospective study was to investigate the clinical factors affecting the occlusal re-establishment and short-term complications of RBFPDPs cemented at an increased OVD.

    MATERIAL AND METHODS: Occlusal re-establishment and clinical complications were reviewed in 109 participants (155 RBFPDPs) treated at the Faculty of Dentistry, Universiti Teknologi MARA from January 2013 to May 2018. Types of complications and risk factors were assessed from clinical data. Demographic factors, clinical factors, and prosthesis factors were distinguished and their association with occlusal re-establishment analyzed by using multiple regression analysis.

    RESULTS: A total of 155 RBFPDPs (42 anterior, 113 posterior) were placed in 109 participants. All achieved occlusal re-establishment, 89.9% complete occlusal re-establishment and 10.1% partial occlusal re-establishment, a minimum of 4 months after the RBFPDPs had been placed at an increased OVD. The design of the metal retainer coverage was found to be a significant factor affecting occlusal re-establishment and decementation (P

  5. Lim TW, Idris RI, Mahmud M
    Clin Exp Dent Res, 2023 Dec;9(6):1089-1095.
    PMID: 37622386 DOI: 10.1002/cre2.774
    OBJECTIVE: Resin-bonded fixed dental prostheses (RBFDPs) cemented at an increased occlusal vertical dimension (OVD) (the Dahl concept) to create space for a metal retainer remains controversial because of the lack of reported clinical studies. This study analyzed the demographic (age and sex) and clinical factors (location and arch of prosthesis) affecting the patients' perception of RBFDPs cemented at an increased OVD.

    MATERIAL AND METHODS: Twenty-eight participants treated with cantilevered RBFDP at an increased OVD were prospectively recruited. They were asked to answer a validated patient satisfaction questionnaire based on six parameters during the 12-week review visit.

    RESULTS: 71.4% of the participants were completely satisfied with the color, shape, and function. Twenty-one (75%) participants reported no complaints about the prostheses. 89.3% will recommend this treatment option to others. There was a significant difference between males and females in avoiding loading on the prostheses (p = 0.015). The level of satisfaction did not differ by age, ethnicity, location, and arch of the prostheses (p > 0.05).

    CONCLUSIONS: Patient satisfaction toward RBFDP cemented by using the Dahl approach was generally high on all the parameters at the 12-week review visit.

  6. Fang R, Lo E, Lim TW
    PMID: 6740379
    In 1982, Malaysia experienced the worst dengue/dengue haemorrhagic fever outbreak in its history. All states in Peninsular and East Malaysia were similarly affected. There was a total of 3,005 cases with 35 deaths, with the majority of cases occurring between the months of July to October. There was a total of 1,001 laboratory confirmed cases. Most of the cases were in patients over the age of 15 years. The Chinese population was mainly affected, although a much higher proportion of Malays was noted in comparison to previous years. The main serotypes involved were dengue-1 and dengue-3. No dengue-4 serotype were isolated.
  7. Idris RI, Shoji Y, Lim TW
    J Prosthet Dent, 2021 Jan 14.
    PMID: 33455729 DOI: 10.1016/j.prosdent.2020.11.035
    STATEMENT OF PROBLEM: Space creation for a metal retainer of the resin-bonded fixed partial dental prostheses (RBFPDPs) with the Dahl concept remains controversial because of the lack of clinical studies.

    PURPOSE: The purpose of this clinical study was to investigate the occlusal force and occlusal contact reestablishment of RBFPDPs cemented at an increased occlusal vertical dimension (the Dahl concept) and to evaluate the factors affecting them.

    MATERIAL AND METHODS: A prospective clinical study was carried out on 28 participants receiving cantilevered RBFPDPs at an increased occlusal vertical dimension at the Faculty of Dentistry, University Teknologi MARA. Maximum occlusal forces were recorded at precementation, postcementation, and 12-week review visit by using pressure indicating film, while occlusal contact reestablishment was assessed at the precementation stage and 12-week review visit by using shim stock foils. The Wilcoxon signed-rank and chi-squared tests were used for statistical analysis (α=.05).

    RESULTS: Significant differences were found for maximum occlusal force between the precementation and the postcementation and between the postcementation and the 12-week review of RBFPDPs (P.05).

    CONCLUSIONS: Overall, occlusal force was reestablished after 12 weeks, and occlusal contact was completely reestablished in most participants after placement of RBFPDPs at an increased occlusal vertical dimension.

  8. Ab Ghani SM, Abdul Hamid NF, Lim TW
    Eur J Dent Educ, 2021 Aug 17.
    PMID: 34403561 DOI: 10.1111/eje.12712
    INTRODUCTION: Blended learning utilizes technology with reduced face-to-face time and promotes a student-centred learning environment that excites the learning process. However, studies on blended learning in fixed prosthodontics is still lacking. This study aimed to compare students' performance in easy and difficult level of fixed prosthodontic preclinical projects given by either blended learning or conventional teaching.

    METHODS: A cross-sectional study was conducted on 72 s-year dental students, who attended preclinical fixed prosthodontic training. Participants were randomly segregated into conventional teaching (n = 36) and blended learning (n = 36). All participants were evaluated for learning preferences using Visual-Aural-Read/Write-Kinesthetic (VARK) questionnaire and performed a project as their baseline skill assessment. They performed another two preclinical projects (easy and difficult level) after the allocated teaching approach. Learning preferences were analysed using Fisher's exact test and performance in preclinical projects were analysed with an independent t test (significant at p  .05) between groups. No significant differences found between both teaching approaches for easy (p = .319) and difficult projects (p = .339). In the blended learning group, no significant difference was found in both difficulty level of projects (p = .064).

    CONCLUSION: The participants performed equally on both teaching approaches. However, blended learning for preclinical fixed prosthodontics is anticipated as the new norm of learning, especially in the current pandemic with reduced face-to-face time.

  9. Steinberg AG, Lai LYC, Vos GH, Singh RB, Lim TW
    Am J Hum Genet, 1961 Dec;13:355-71.
    PMID: 13916666
    The ABO, MN and Rh blood types, and the Hp, Tf, and Gm [Gm (a), Gm (x), Gm(b), and Gm-like] factors were determined for 128 unrelated Indians (parents of families, 63 with two parents tested and two with one parent tested), and 90 unrelated Chinese (parents of 46 families, 44 with two parents tested and two with one parent tested), and for the offspring from these families. The frequencies of the several blood types are presented. They were done primarily to aid in paternity testing. They compare favorably with the findings of previous studies. The allele Hp1 is rare in the Indian population (.09) and relatively infrequent in the Chinese (.29). Unfortunately, the data shed no light on the problem of the inheritance of the phenotype Hp O. Only Tf C was found among the Indians. About four per cent of the Chinese were heterozygous for Tf CD,, all other were Tf CC. The Indians have a high frequency of Gm(a) and of Gm (x), and a low frequency of Gm (b). They appear to have alleles Gma, Gmax, and Gmb in the following frequencies: .535, .234(5), and .230(5), respectively. Three families appear to have a GMxb allele, providing the offspring are not extra-marital. The Chinese appear to have the alleles Gm^ab, Gm^a, and Gm^ax in the following frequencies: .741, .231, and .028, respectively.
  10. Ong TK, Harun N, Lim TW
    Eur Endod J, 2019;4(2):90-95.
    PMID: 32161894 DOI: 10.14744/eej.2019.13007
    In this case report, three teeth with complete or incomplete cemental tear in two patients were presented. Even though periapical radiograph could detect cemental tear in these three teeth, the cone-beam computed tomography scanning clearly revealed the pattern of the cemental tear, which was later confirmed by histopathological examination. Therefore, this case report shows the benefits of incorporating both cone-beam computed tomography and histopathological examination to diagnose cemental tear.
  11. Rahimi SN, Kassim MZ, Shamsul Anuar SA, Ab Ghani SM, Baharuddin IH, Lim TW
    J Indian Prosthodont Soc, 2018 Oct;18(Suppl 1):S11-S12.
    PMID: 30532402 DOI: 10.4103/0972-4052.244600
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