Displaying publications 1 - 20 of 148 in total

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  1. Growth, sex reversal pattern, and reproductive characteristics of Barramundi (Lates calcarifer) broodstock candidates reared in floating cages
    Che-Zulkifli CI, Akil MAMM, Amin-Safwan A, Mahsol HH, Al-Ghadi MQ, Swelum AA, et al.
    Anim Biotechnol, 2023 Dec;34(8):4126-4134.
    PMID: 37830156 DOI: 10.1080/10495398.2023.2267621
    Sex reversal of male to female is a characteristic of barramundi (Lates calcarifer), which is affected by several factors, thereby changing the broodstock population. A study was conducted in floating cages in Langkawi, Malaysia, to determine the weight point at the onset of the sex reversal phenomena. A total of 75 female and 55 male adult individuals (3-4 weeks of age) were sampled from the fish cultured in cages to ascertain their sex at different weights. The water temperature and salinity values were 29.82 °C and 33.12 ppt, respectively. The specimens were classified into twelve bodyweight classes (2.00-8.00 ± 0.5 kg intervals). Female specimen body weight distribution was highest in the 6.01-6.50 kg class (22.6%), followed by the 5.51-6.00 kg and 4.51-5.00 class (13.3%), while male specimen body weight distribution was highest in the 4.51-5.00 kg class (32.1%), followed by the 4.01-4.50 kg class (30.3%). Length-to-weight relationships for females and males of Asian Seabass indicated positive allometric growth. The correlation between body weight and GSI, using Pearson's correlation, for both sexes, for the male and female barramundi, there was a weak correlation between body weight and GSI, which was 37 and 30%, respectively. Based on the present study's findings, it can be concluded that sex reversal from male to female in Barramundi largely occurred at 4.57 kg body weight and 66.8 cm total length.
    Matched MeSH terms: Perciformes*
  2. Microbiota composition of captive bluestreak cleaner wrasse Labroides dimidiatus (Valenciennes, 1839)
    Nurul AAN, Danish-Daniel AM, Okomoda VT, Asma NA
    Appl Microbiol Biotechnol, 2020 Sep;104(17):7391-7407.
    PMID: 32676710 DOI: 10.1007/s00253-020-10781-y
    The Labroides dimidiatus is one of the most traded marine ornamental fishes worldwide, yet not much is known about the microflora associated with this fish. This study is designed to investigate the bacteria composition associated with captive L. dimidiatus and its surrounding aquarium water. The fish and carriage water were obtained from well-known ornamental fish suppliers in Terengganu Malaysia. Bacteria present on the skin and in the stomach and the aquarium water were enumerated using culture-independent approaches and next-generation sequencing (NGS) technology. A total of 3,238,564 valid reads and 828 total operational taxonomic units (OTUs) were obtained from the three metagenomic libraries using NGS analysis. Of all the 15 phyla identified in this study, Proteobacteria, Bacteroidetes, Firmicutes, and Actinobacteria were the most prevalent in all samples. Also, 170 families belonging to 36 bacteria classes were identified. Although many of the bacteria families were common in the skin, gut, and aquarium water (39%), about 26% of the families were exclusive to the aquarium water alone. Therefore, any substantial change in the structure and abundance of microbiota (especially pathogenic bacteria) reported in this study may serve as an early sign for disease infection in the species under captivity. KEY POINTS: • Proteobacteria was the most dominant. • The microbiota was either shared or exclusively in samples.
    Matched MeSH terms: Perciformes*
  3. Identification of the major allergens of Indian scad (Decapterus russelli)
    Misnan R, Murad S, Jones M, Taylor G, Rahman D, Arip M, et al.
    Asian Pac J Allergy Immunol, 2008 Dec;26(4):191-8.
    PMID: 19317337
    The purpose of this study was to characterize major allergens of Indian scad (Decapterus russelli) which is among the most commonly consumed fish in Malaysia. Raw and cooked extracts of the fish were prepared. Protein profiles and IgE binding patterns were produced by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting using sera from subjects with fish allergy. The major allergens of the fish were then identified by two-dimensional electrophoresis (2-DE), followed by mass spectrometry of the peptide digests. The SDS-PAGE of the raw extract revealed 27 protein fractions over a wide molecular weight range, while the cooked extract demonstrated only six protein fractions. The 1-DE immunoblotting detected 14 IgE-binding proteins, with a molecular weight range from 90 to < 6.5 kDa. Three protein fractions with molecular weights of approximately 51, 46 and 12 kDa were identified as the major allergens of this fish. The approximately 12 kDa band was a heat-resistant protein while the approximately 51 and 46 kDa proteins were sensitive to heat. The 2-DE gel profile of the raw extract demonstrated > 100 distinct protein spots and immunoblotting detected at least 10 different major IgE reactive spots with molecular masses as expected and isoelectric point (pI) values ranging from 4.0 to 7.0. A comparison of the major allergenic spot sequences of the 12 kDa proteins with known protein sequences in databases revealed extensive similarity with fish parvalbumin. In conclusion, this study demonstrated that a parvalbumin which is similar to Gad c 1 is the major allergen of Indian scad. Interestingly, we also detected heat-sensitive proteins as major allergenic components in our fish allergy patients.
    Matched MeSH terms: Perciformes/immunology*; Perciformes/metabolism
  4. Fulltext Prevalence, antimicrobial susceptibility and plasmid profiling of Vibrio spp. isolated from cultured groupers in Peninsular Malaysia
    Amalina NZ, Santha S, Zulperi D, Amal MNA, Yusof MT, Zamri-Saad M, et al.
    BMC Microbiol, 2019 11 11;19(1):251.
    PMID: 31711432 DOI: 10.1186/s12866-019-1624-2
    BACKGROUND: Numerous prevalence studies of Vibrio spp. infection in fish have been extensively reported worldwide, including Malaysia. Unfortunately, information on the prevalence of Vibrio spp. in groupers (Epinephelus spp.) is limited. In this study, groupers obtained from nine farms located at different geographical regions in Malaysia were sampled for the presence of pathogenic Vibrio spp. and their susceptibility profiles against seven antibiotics.

    RESULTS: Out of 270 grouper samples, 195 (72%) were detected with the presence of Vibrio spp. Vibrio communis showed highest prevalence in grouper (28%), followed by V. parahaemolyticus (25%), V. alginolyticus (19%), V. vulnificus (14%), V. rotiferianus (3%), Vibrio sp. (3%), V. campbellii (2%), V. mytili (2%), V. furnissii (2%), V. harveyi (1%), V. tubiashii (1%), V. fluvialis (0.3%) and V. diabolicus (0.3%). Assessment on the antibiotic susceptibility profiles of the Vibrio spp. revealed that majority of the isolates were susceptible to tetracycline, streptomycin, erythromycin and bacitracin, but resistance to ampicillin, penicillin G and vancomycin. The mean MAR index of the Vibrio isolates was 0.51, with 85% of the isolates showed MAR index value of higher than 0.2. Results indicate that the Vibrio spp. were continuously exposed to antibiotics. Furthermore, the plasmid profiles of Vibrio spp. showed that 38.7% of the isolates harbored plasmid with molecular weight of more than 10 kb, while 61.3% were without plasmid. During curing process, Vibrio spp. lost their plasmid, but remained resistant to ampicillin, penicillin G, bacitracin and vancomycin while a few isolates remained resistant to erythromycin, streptomycin and tetracycline. The results suggested that the resistance to antibiotics in isolated Vibrio spp. might be due to chromosomal and plasmid borne.

    CONCLUSIONS: This study demonstrates the prevalence of Vibrio spp. in groupers and the distribution of multidrug resistance strains that could be of concern to the farmers in Malaysia. In addition, data from this study can be further used in fish disease management plan.

    Matched MeSH terms: Perciformes/microbiology*
  5. The capacity for long-chain polyunsaturated fatty acid synthesis in a carnivorous vertebrate: Functional characterisation and nutritional regulation of a Fads2 fatty acyl desaturase with Δ4 activity and an Elovl5 elongase in striped snakehead (Channa striata)
    Kuah MK, Jaya-Ram A, Shu-Chien AC
    Biochim. Biophys. Acta, 2015 Mar;1851(3):248-60.
    PMID: 25542509 DOI: 10.1016/j.bbalip.2014.12.012
    The endogenous production of long-chain polyunsaturated fatty acids (LC-PUFA) in carnivorous teleost species inhabiting freshwater environments is poorly understood. Although a predatory lifestyle could potentially supply sufficient LC-PUFA to satisfy the requirements of these species, the nutrient-poor characteristics of the freshwater food web could impede this advantage. In this study, we report the cloning and functional characterisation of an elongase enzyme in the LC-PUFA biosynthesis pathway from striped snakehead (Channa striata), which is a strict freshwater piscivore that shows high deposition of LC-PUFA in its flesh. We also functionally characterised a previously isolated fatty acyl desaturase cDNA from this species. Results showed that the striped snakehead desaturase is capable of Δ4 and Δ5 desaturation activities, while the elongase showed the characteristics of Elovl5 elongases. Collectively, these findings reveal that striped snakehead exhibits the genetic resources to synthesise docosahexaenoic acid (DHA; 22:6n-3) from eicosapentaenoic acid (EPA; 20:5n-3). Both genes are expressed at considerable levels in the brain and the liver. In liver, both genes were up-regulated by dietary C18 PUFA, although this increase did not correspond to a significant rise in the deposition of muscle LC-PUFA. Brain tissue of fish fed with plant oil diets showed higher expression of fads2 gene compared to fish fed with fish oil-based diet, which could ensure DHA levels remain constant under limited dietary DHA intake. This suggests the importance of DHA production from EPA via the ∆4 desaturation step in order to maintain an optimal reserve of DHA in the neuronal tissues of carnivores.
    Matched MeSH terms: Perciformes/classification; Perciformes/genetics; Perciformes/metabolism*
  6. Fulltext Checklist of the coral fish fauna of Xisha Islands, China
    Qiu S, Chen B, Du J, Loh KH, Liao J, Liu X, et al.
    Biodivers Data J, 2021;9:e63945.
    PMID: 33732033 DOI: 10.3897/BDJ.9.e63945
    Background: The Xisha Islands are composed of the Yongle Islands and the Xuande Islands in Hainan Province, China. It has one of the highest species diversity in the world and is also a typical oceanic distribution area of coral reefs globally. The ichthyofauna of the Xisha Islands were recorded by underwater visual census in May 2019 and July 2020. The survey data were combined with previous records of species into the checklist of the Xisha Islands presented herein. A total of 691 species, belonging to 24 orders and 97 families, was recorded. The major families were Labridae, Pomacentridae, Serranidae, Chaetodontidae, Hexanchidae, Lutjanidae, Scaridae, Gobiidae, Scorpaenidae and Carangidae. In this study, the Coral Fish iversity Index (CFDI) of six families (Chaetodontidae, Pomacanthidae, Pomacentridae, Labridae, Scaridae and Acanthuridae) was 229, indicating 756 coral fishes. In terms of the IUCN Red List, one species is Critically Endangered (Glyphis gangeticus), six species are Endangered (Stegostoma fasciatum, Aetomylaeus maculatus, Aetomylaeus vespertilio, Epinephelus akaara, Cheilinusundulatus sp. and Xiphias gladius), 16 species are Vulnerable, and 13 species are Near Threatened in the Xisha Archipelago, so conservation should be strengthened in this area in the future.

    New information: One species is a new record for China (Dischistodus pseudochrysopoecilus) and 23 species are newly found in the Xisha Islands.

    Matched MeSH terms: Perciformes
  7. Potentially Toxic Elements (PTEs) in the Fillet of Narrow-Barred Spanish Mackerel (Scomberomorus commerson): a Global Systematic Review, Meta-analysis and Risk Assessment
    Thai VN, Dehbandi R, Fakhri Y, Sarafraz M, Nematolahi A, Dehghani SS, et al.
    Biol Trace Elem Res, 2021 Sep;199(9):3497-3509.
    PMID: 33180263 DOI: 10.1007/s12011-020-02476-2
    The contamination of seafood like narrow-barred Spanish mackerel (Scomberomorus commerson) fillets by potentially toxic elements (PTEs) has converted to worldwide health concerns. In this regard, the related citations regarding the concentration of PTEs in fillets of narrow-barred Spanish mackerel were collected through some of the international databases such as Scopus, Cochrane, PubMed, and Scientific Information Database (SID) up to 10 March 2020. The concentration of PTEs in fillets of narrow-barred Spanish mackerel fish was meta-analyzed and the health risk (non-carcinogenic risk) was estimated by the total target hazard quotient (TTHQ). The meta-analysis of data indicated that the rank order of PTEs in fillet of narrow-barred Spanish mackerel was Fe (10,853.29 μg/kg-ww) > Zn (4007.00 μg/kg-ww) > Cu (1005.66 μg/kg-ww) > total Cr (544.14 μg/kg-ww) > Mn (515.93 μg/kg-ww) > Ni (409.90 μg/kg-ww) > Pb (180.99 μg/kg-ww) > As (93.11 μg/kg-ww) > methyl Hg (66.60 μg/kg-ww) > Cd (66.03 μg/kg-ww). The rank order of health risk assessment based on the country by the aid of TTHQ for adult consumers was Malaysia (0.22251) > Philippines (0.21912) > Egypt (0.08684) > Taiwan (0.07430) > Bahrain (0.04893) > Iran (0.03528) > China (0.00620) > Pakistan (0.00316) > Yemen (0.00157) > India (0.00073). In addition, the rank order of health risk assessment based on the country by the aid of TTHQ for child consumers was Malaysia (1.03838) > Philippines (1.02257) > Egypt (0.40523) > Taiwan (0.34674) > Bahrain (0.22832) > Iran (0.16466) > China (0.02892) > Pakistan (0.01474) > Yemen (0.00731) > India (0.00340). Therefore, the children in Malaysia and the Philippines were at considerable non-carcinogenic risk. Hence, approaching the recommended control plans in order to decrease the non-carcinogenic risk associated with the ingestion of PTEs via the consumption of narrow-barred Spanish mackerel fish fillets is crucial.
    Matched MeSH terms: Perciformes*
  8. Mercury Bioaccumulation in Tropical Mangrove Wetland Fishes: Evaluating Potential Risk to Coastal Wildlife
    Le DQ, Satyanarayana B, Fui SY, Shirai K
    Biol Trace Elem Res, 2018 Dec;186(2):538-545.
    PMID: 29577182 DOI: 10.1007/s12011-018-1313-2
    The present study, aimed at observing the total concentration of mercury (Hg) in edible finfish species with an implication to human health risk, was carried out from the Setiu mangrove wetlands on the east coast of Peninsular Malaysia. Out of 20 species observed, the highest Hg concentrations were found among carnivores-fish/invertebrate-feeders, followed by omnivores and carnivores-invertebrate-feeders, while the lowest concentrations in herbivores. The Hg concentrations varied widely with fish species and body size, from 0.12 to 2.10 mg/kg dry weight. A positive relationship between body weight and Hg concentration was observed in particular for Toxotes jaculatrix and Tetraodon nigroviridis. Besides the permissible range of Hg concentration up to 0.3 mg/kg (cf. United States Environmental Protection Agency (USEPA)) in majority of species, the carnivore feeders such as Acanthopagrus pacificus, Gerres filamentosus, and Caranx ignobilis have shown excess amounts (> 0.40 mg/kg flesh weight) that raising concerns over the consumption by local people. However, the weekly intake of mercury-estimated through the fish consumption in all three trophic levels-suggests that the present Hg concentrations are still within the range of Provisional Tolerable Weekly Intake (PTWI) reported by the Joint FAO/WHO Expert Committee on Food Additives (JECFA). Perhaps, a multi-species design for Hg monitoring at Setiu wetlands would be able to provide further insights into the level of toxicity transfer among other aquatic organisms and thereby a strong health risk assessment for the local communities.
    Matched MeSH terms: Perciformes/classification
  9. The Presence of Microplastics in the Gills and Gastrointestinal Tract of Mackerel (Rastrelliger Kanagurta Cuvier, 1816) from Jakarta Bay, Indonesia
    Irnidayanti Y, Soegianto A, Brabo AH, Abdilla FM, Putranto TWC, Payus CM
    Bull Environ Contam Toxicol, 2023 Sep 13;111(3):37.
    PMID: 37704797 DOI: 10.1007/s00128-023-03786-6
    This study examined the characteristics of microplastics (MPs) in the gills and gastrointestinal tract (GIT) of mackerel (Rastrelliger kanagurta) captured in Jakarta Bay, Indonesia. All 120 fish contained MPs, with fragment > fiber > film being the most prevalent types, in that order. The total abundances of fragments, fibers, and films in the gills were as follows: 4.8 ± 1.6, 1.0 ± 0.7, and 0.3 ± 0.3, respectively. The total abundances of fragments, fibers, and films in the GIT were 5.9 ± 2.3, 1.3 ± 0.8, and 0.4 ± 0.4, respectively. Statistical analysis showed that the abundance of fragments, fibers and films in both the gills and GIT of mackerel did not differ significantly between sampling locations. In the gills and GIT, MPs with sizes less than 0.1 mm and MPs that were black in color were most prevalent. Fourier transform infrared (FTIR) spectroscopy tests on MPs from the tissues of mackerel showed that there were 8 different types of MP polymers, namely, latex, nylon, polymethyl methacrylate (PMMA), cellulose acetate (CA), polyurethane (PU), polycarbonate (PC), polystyrene (PS), and polytetrafluoroethylene (PTFE). Latex and polycarbonate were detected in fish samples from Jakarta Bay.
    Matched MeSH terms: Perciformes*
  10. Effect of various antagonists on the Channa striatus fillet extract antinociception in mice
    Zakaria ZA, Sulaiman MR, Mat Jais AM, Somchit MN
    Can J Physiol Pharmacol, 2005 Jul;83(7):635-42.
    PMID: 16091789
    The effects of an aqueous supernatant of haruan (ASH) (Channa striatus) fillet extract on various antinociception receptor system activities were examined using a mouse abdominal-constriction model. Mice that were pretreated with distilled water, s.c., followed 10 min later by administration of 25%, 50%, and 100% concentration ASH, s.c., produced a significant concentration-dependent antinociceptive activity (p < 0.001). Pretreatment with naloxone (0.3, 1.0, and 3.0 mg/kg body mass), 10 min before ASH administration, failed to block the extract antinociception. Pretreatment of the 100% concentration ASH with mecamylamine (5 mg/kg), pindolol (10 mg/kg), and haloperidol (1 mg/kg) also did not cause any significant change in its antinociception. However, pretreatment with atropine (5 mg/kg), bicuculline (10 mg/kg), phenoxybenzamine (10 mg/kg), and methysergide (5 mg/kg) were found to reverse ASH antinociception. Based on the above findings, the ASH is suggested to contain different types of bioactive compounds that act synergistically on muscarinic, GABAA, alpha-adrenergic, and serotonergic receptor systems to produce the observed antinociception.
    Matched MeSH terms: Perciformes/physiology*
  11. Bioaccumulation of heavy metals in maricultured fish, Lates calcarifer (Barramudi), Lutjanus campechanus (red snapper) and Lutjanus griseus (grey snapper)
    Nasyitah Sobihah N, Ahmad Zaharin A, Khairul Nizam M, Ley Juen L, Kyoung-Woong K
    Chemosphere, 2018 Apr;197:318-324.
    PMID: 29360594 DOI: 10.1016/j.chemosphere.2017.12.187
    Mariculture fish contains a rich source of protein, but some species may bioaccumulate high levels of heavy metals, making them unsafe for consumption. This study aims to identify heavy metal concentration in Lates calcarifer (Barramudi), Lutjanus campechanus (Red snapper) and Lutjanus griseus (Grey snapper). Three species of mariculture fish, namely, L. calcarifer, L. campechanus and L. griseus were collected for analyses of heavy metals. The concentration of heavy metal (As, Cd, Cu, Cr, Fe, Pb, Mn, Ni, Se, and Zn) was determined using inductive coupled plasma mass spectrometry (ICP-MS). The distribution of heavy metals mean concentration in muscle is Zn > Fe > As > Se > Cr > Cu > Mn > Pb > Ni > Cd for L. calcarifer, Fe > Zn > Cr > As > Ni > Mn > Se > Cu > Pb > Cd for L. campechanus and Fe > Zn > Cr > Ni > Se > Cu > As > Mn > Pb > Cd for L. griseus. Among all of the species under investigation, the highest concentration of Fe was found in the muscle tissue of L. campechanus (19.985 ± 1.773 mg kg-1) and liver tissue of L. griseus (58.248 ± 8.736 mg kg-1). Meanwhile, L. calcarifer has the lowest concentration of Cd in both muscle (0.007 ± 0.004 mg kg-1) and liver tissue (0.027 ± 0.016 mg kg-1). The heavy metal concentration in muscle tissue is below the permissible limit guidelines stipulated by the Food & Agriculture Organization, 1983 and Malaysia Food Act, 1983. The concentration of heavy metals varies significantly among fish species and tissues. L. campechanus was found to have a higher ability to accumulate heavy metals as compared to the other two species (p 
    Matched MeSH terms: Perciformes/metabolism*
  12. Molecular cloning and ontogenic mRNA expression of fatty acid desaturase in the carnivorous striped snakehead fish (Channa striata)
    Jaya-Ram A, Ishak SD, Enyu YL, Kuah MK, Wong KL, Shu-Chien AC
    Comp Biochem Physiol A Mol Integr Physiol, 2011 Apr;158(4):415-22.
    PMID: 21130179 DOI: 10.1016/j.cbpa.2010.11.018
    There is very little information on the capacity of freshwater carnivorous fish to biosynthesize highly unsaturated fatty acids (HUFA). The striped snakehead fish (Channa striata) is a carnivorous species cultured inland of several Southeast Asian countries due to its pharmaceutical properties in wound healing enhancement. We described here the full-length cDNA cloning of a striped snakehead fatty acid desaturase (fads), which is responsible for desaturation of unsaturated fatty acids in the HUFA biosynthesis. Bioinformatics analysis reveals a protein coding region with length of 445 amino acids containing all characteristic features of desaturase enzyme, including a cytochrome b5-domain with the heme-binding motif, two transmembrane domains and three histidine-rich regions. The striped snakehead fads amino acid sequence shares high similarity with known fads of other teleosts. The mRNA expression of striped snakehead fads also showed an ontogenic-related increase in expression in 0-20 days after hatch larva. Using ISH, we localized the presence of fads in larva brain, liver and intestinal tissues.
    Matched MeSH terms: Perciformes/genetics*; Perciformes/growth & development; Perciformes/metabolism
  13. A fatty acyl desaturase (fads2) with dual Δ6 and Δ5 activities from the freshwater carnivorous striped snakehead Channa striata
    Kuah MK, Jaya-Ram A, Shu-Chien AC
    Comp Biochem Physiol A Mol Integr Physiol, 2016 11;201:146-155.
    PMID: 27421235 DOI: 10.1016/j.cbpa.2016.07.007
    There is a lack of understanding on how the environment and trophic niche affect the capability of long-chain polyunsaturated fatty acids (LC-PUFA) in freshwater carnivorous teleost. In this present study, we isolated and functionally characterised a fatty acyl desaturase (Fads) from the striped snakehead Channa striata. Sequence comparison and phylogenetic analysis suggested a Fads2 protein that is closely related to previously characterised Fads2 proteins from freshwater carnivorous and marine herbivorous fish species. We further demonstrated the capacity of Δ6 and Δ5 desaturation activities for this particular desaturase, with highest activities towards the conversion of omega-3 (n-3) polyunsaturated fatty acids (PUFA). Low Δ4 desaturation activity was also detected, although the significance of this at a physiological level remains to be studied. The expression of this striped snakehead Δ6/Δ5 fads2 gene was highest in brain, followed by liver and intestine. In liver, diet fortified with high LC-PUFA concentration impeded the expression of Δ6/Δ5 fads2 gene compared to vegetable oil (VO) based diets. The discovery of Δ6/Δ5 Fads2 desaturase here complements the previous discovery of a Δ4 Fads2 desaturase and an Elovl5 elongase, lending proof to the existence of all the required enzymatic machinery to biosynthesise LC-PUFA from C18 PUFA in a freshwater carnivorous species.
    Matched MeSH terms: Perciformes/genetics*; Perciformes/metabolism*
  14. Iridovirus disease in two ornamental tropical freshwater fishes: African lampeye and dwarf gourami
    Sudthongkong C, Miyata M, Miyazaki T
    Dis Aquat Organ, 2002 Apr 5;48(3):163-73.
    PMID: 12033703
    Many species of ornamental freshwater fishes are imported into Japan from all over the world. We found African lampeye Aplocheilichthys normani and dwarf gourami Colisa lalia suffering from an iridovirus infection just after being imported by tropical fish wholesalers from Singapore. African lampeye were cultured on the Indonesian Island of Sumatra and dwarf gourami were cultured in Malaysia before export. Diseased fishes displayed distinct histopathological signs of iridovirus infection: systemic appearance of inclusion body-bearing cells, and necrosis of splenocytes and hematopoietic cells. Electron microscopy revealed viral particles (African lampeye:180 to 200 nm in edge to edge diameter; dwarf gourami: 140 to 150 nm in diameter) in an inclusion body within the cytoplasm of inclusion body-bearing cells as well as in the cytoplasm of necrotized cells. Experimental infection with an iridovirus isolate from African lampeye (ALIV) revealed pathogenicity of ALIV to African lampeye and pearl gourami Trichogaster leeri. Polymerase chain reaction (PCR) products from ALIV and an iridovirus isolate from dwarf gourami (DGIV) using iridovirus-specific primers were indistinguishable. The nucleotide sequence of PCR products derived from ALIV (696 base pairs) and DGIV (701 base pairs) had 95.3% identity. These results indicate that ALIV and DGIV have a single origin.
    Matched MeSH terms: Perciformes*
  15. Effects of skin abrasion in immersion challenge with Vibrio harveyi in Asian seabass Lates calcarifer fingerlings
    Chin YK, Ina-Salwany MY, Zamri-Saad M, Amal MNA, Mohamad A, Lee JY, et al.
    Dis Aquat Organ, 2020 Jan 16;137(3):167-173.
    PMID: 31942862 DOI: 10.3354/dao03435
    Skin abrasions often occur in farmed fish following handling by labourers, injury by farm facilities, cannibalism and ectoparasites. Vibrio spp. are opportunistic pathogens that can invade host fish through damaged tissues and cause outbreaks of vibriosis. This study describes the effect of skin abrasions on the infectivity of V. harveyi using Asian seabass Lates calcarifer (Bloch, 1790) fingerlings as a case example and compares bacterial load and fish survival following immersion challenge with different doses. In total, 315 fish (6.67 ± 1.8 g) were divided into 3 treatments: skin abrasion followed by immersion infection, immersion infection only and an uninfected, uninjured control. Fish in the infection treatments were divided into 3 subgroups and exposed in triplicate to a 7 d immersion challenge with 106, 107 and 108 CFU ml-1 of live V. harveyi. No mortalities were observed in the control and immersion infection groups. However, fish in the skin abrasion treatment group that were infected with 108 CFU ml-1 of live V. harveyi showed signs of progressing disease throughout the experiment, which resulted in mortalities. Significantly higher bacterial loads (p < 0.05) were recorded in the intestine, liver and gills of the fish in this group. Fish in the skin abrasion treatment that were exposed to 107 and 108 CFU ml-1 of V. harveyi showed 100% mortality by Days 5 and 4, respectively. These findings confirm that skin injuries increase the susceptibility of seabass fingerlings to V. harveyi infection.
    Matched MeSH terms: Perciformes*
  16. Biomagnifications of mercury and methylmercury in tuna and mackerel
    Hajeb P, Jinap S, Ahmad I
    Environ Monit Assess, 2010 Dec;171(1-4):205-17.
    PMID: 20041345 DOI: 10.1007/s10661-009-1272-3
    Seawater may be contaminated by harmful substances, including toxic elements released by human activities. The present study evaluates the total mercury and methylmercury concentrations and their correlations to fish body size in longtail tuna and short-bodied mackerel from Chendring, Kuantan, at east coast and Kuala Perlis at west costs of Peninsular Malaysia during May to November 2007. Total mercury and methylmercury in muscle tissue of 69 samples of longtail tuna and short-bodied mackerel, ranged from 0.180 to 1.460 μg/g and 0.0.169-0.973 μg/g and 0.251-1.470 μg/g and 0.202-1.352, whereas the methylmercury to total mercury ratio ranged from 70% to 83%, respectively. Samples of both species from the east coast showed higher levels of mercury compared to those from west coast. In all of the locations, significant positive correlations were found between fish body weight and mercury content (R(2) > 0.470). The estimated weekly intake of total mercury and methylmercury from the consumption 66.33 g/week of short-bodied mackerel and 18.34 g/week of longtail tuna (based on local dietry survey) was found to be lower than the maximum limit of 5 and 1.5 μg/kg bodyweight established by FAO/WHO and codex, respectively.
    Matched MeSH terms: Perciformes*
  17. Antidepressant-like effect of aqueous extract of Channa striatus fillet in mice models of depression
    Saleem AM, Taufik Hidayat M, Mat Jais AM, Fakurazi S, Moklas M, Sulaiman MR, et al.
    Eur Rev Med Pharmacol Sci, 2011 Jul;15(7):795-802.
    PMID: 21780549
    Channa (C.) striatus (Malay-Haruan), is a fresh water snakehead fish, consumed as a rejuvenating diet in post-parturition period in local Malay population. The aqueous extract of C. striatus fillet (AECSF) was reported to act through serotonergic receptor system in a previous study. There is no scientific report on neuropharmacological effects of C. striatus. Based on these data, the antidepressant-like effect of C. striatus was evaluated in mice models of depression.
    Matched MeSH terms: Perciformes*
  18. Parabrachiella jarai sp. nov. (Crustacea: Copepoda: Siphonostomatoida) parasitic on Sillago sihama (Actinopterygii: Perciformes: Sillaginidae)
    Piasecki W, Młynarczyk M, Hayward CJ
    Exp Parasitol, 2010 May;125(1):55-62.
    PMID: 19850037 DOI: 10.1016/j.exppara.2009.10.001
    The presently reported study provides a detailed morphological description of the female and the male of a new species of the genus Parabrachiella-Parabrachiella jarai sp. nov. The parasites were sampled from marine fish, silver sillago, Sillago sihama (Perciformes: Sillaginidae), captured in Malaysia in 1994 and Hong Kong in 1995. The new species bears some resemblance to Parabrachiella lata (Song et Chen, 1976) but differs from it in details of second antenna, mandible, and maxilliped. The genus Parabrachiella currently covers 67 species including those recently transferred from Neobrachiella Kabata, 1979. An amended generic diagnosis is proposed for Parabrachiella and Thysanote. Some members of Parabrachiella are herewith transferred to Thysanote and some Thysanote are now placed in Parabrachiella.
    Matched MeSH terms: Perciformes/parasitology*
  19. Use of cestodes as indicator of heavy-metal pollution
    Yen Nhi TT, Mohd Shazili NA, Shaharom-Harrison F
    Exp Parasitol, 2013 Jan;133(1):75-9.
    PMID: 23146722 DOI: 10.1016/j.exppara.2012.10.014
    Thirty snakehead fish, Channa micropeltes (Cuvier, 1831) were collected at Lake Kenyir, Malaysia. Muscle, liver, intestine and kidney tissues were removed from each fish and the intestine was opened to reveal cestodes. In order to assess the concentration of heavy metal in the environment, samples of water in the surface layer and sediment were also collected. Tissues were digested and the concentrations of manganese (Mn), zinc (Zn), copper (Cu), cadmium (Cd) and lead (Pb) were analysed by using inductively-coupled plasma mass-spectrometry (ICP-MS) equipment. The results demonstrated that the cestode Senga parva (Fernando and Furtado, 1964) from fish hosts accumulated some heavy metals to a greater extent than the water and some fish tissues, but less than the sediment. In three (Pb, Zn and Mn) of the five elements measured, cestodes accumulated the highest metal concentrations, and in remaining two (Cu and Cd), the second highest metal accumulation was recorded in the cestodes when compared to host tissues. Therefore, the present study indicated that Senga parva accumulated metals and might have potential as a bioindicator of heavy-metal pollution.
    Matched MeSH terms: Perciformes/metabolism; Perciformes/parasitology*
  20. Fulltext Phenotypic and genetic characterizations of Streptococcus dysgalactiae strains isolated from fish collected in Japan and other Asian countries
    Abdelsalam M, Chen SC, Yoshida T
    FEMS Microbiol Lett, 2010 Jan;302(1):32-8.
    PMID: 19895643 DOI: 10.1111/j.1574-6968.2009.01828.x
    Lancefield group C Streptococcus dysgalactiae is an emerging fish pathogen, which was first isolated in 2002 in Japan. Streptococcus dysgalactiae isolates collected from diseased fish in Japan (n=12), Taiwan (n=12), China (n=2), Malaysia (n=3), and Indonesia (n=1) were characterized using biased sinusoidal field gel electrophoresis (BSFGE), sodA gene sequence analysis, and antimicrobial susceptibility. These isolates exhibited high phenotypic homogeneity irrespective of the countries from where the strains were collected. Seventeen isolates were found to be resistant to oxytetracycline and carried the tet(M) gene, except for the strains collected in Taiwan and the PP1564 strain collected in China. The sodA gene sequence analysis revealed that 23 isolates were identical, except for one Japanese isolate (KNH07902), in which a single nucleotide differed from that of the other isolates. Based on BSFGE typing by ApaI macrorestriction, the isolates - including the Japanese, Taiwanese, and Chinese isolates - could be grouped into one main cluster at a 70% similarity level. However, the macrorestriction genotypes of some isolates were apparently distinct from those of the main cluster.
    Matched MeSH terms: Perciformes/microbiology*
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