Displaying publications 221 - 240 of 1467 in total

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  1. Amoudi MA
    J. Protozool., 1988 Feb;35(1):116-8.
    PMID: 3367316
    The following species are described from Indonesian birds: Isospora paddae n. sp. with oocysts 41.5-45.5 x 40.3-41.5 (44 +/- 1.15 x 41.2 +/- 0.38) and sporocysts 22.8-24.5 x 14.7-17 (24 +/- 0.55 x 16.2 +/- 0.81) from the Java sparrow, Padda oryzivora, and Isospora indonesianensis n. sp. with oocysts 39.3-43.6 x 37-40.8 (41.8 +/- 1.3 x 39.6 +/- 1.25) and sporocysts 25.6-28.4 x 15.2-18.5 (27.1 +/- 1.05 x 16.8 +/- 1.22) from the chestnut Munia, Lonchura malacca (L). The host birds belong to the order Passerorida.
    Matched MeSH terms: Bird Diseases/parasitology*; Coccidiosis/parasitology
  2. Prakash BK, Low VL, Vinnie-Siow WY, Tan TK, Lim YA, Morvarid AR, et al.
    J Med Entomol, 2018 Aug 29;55(5):1337-1340.
    PMID: 29762747 DOI: 10.1093/jme/tjy072
    Canine babesiosis is an emerging tick-borne disease with a worldwide distribution, including Malaysia. While the prevalence of Babesia has been documented from dogs in Malaysia, occurrence of Babesia has been relatively little studied in their tick vectors. Accordingly, a total of 240 dogs and 140 Rhipicephalus sanguineus sensu lato (s.l.) (Acari: Ixodidae) ticks from Malaysia were molecularly screened for the presence of Babesia protozoa in the present study. Babesia gibsoni was only detected in ticks (1.4%), whereas Babesia vogeli was detected in both ticks (1.4%) and dogs (2.1%). This study highlights the detection of B. gibsoni and B. vogeli for the first time, in both adult and nymphal stages of R. sanguineus s.l. in Malaysia, suggesting the potential role of this tick species in transmitting canine babesiosis.
    Matched MeSH terms: Dogs/parasitology*; Rhipicephalus sanguineus/parasitology*
  3. Vythilingam I, Boaz L, Wa N
    J Am Mosq Control Assoc, 1998 Sep;14(3):243-7.
    PMID: 9813819
    Accurate identification of filarial parasites in mosquitoes poses a major problem for the coordination of filariasis control programs. Traditional methods are tedious, and some are not specific enough to give satisfactory results. Amplification of specific gene sequences by primer-directed polymerase chain reaction (PCR) has been increasingly utilized as a diagnostic tool. However, current protocols for the extraction of parasite DNA from mosquito samples are tedious and could lead to failure of PCR amplification. We demonstrate that the use of Chelex is an efficient method for DNA extraction from mosquitoes and the parasite and that PCR amplification with primers specific for Brugia malayi yields a band of the expected size. The PCR products were transferred to a nylon membrane with Southern blotting, and a B. malayi-specific digoxigenin-labeled probe confirmed the sequence similarity of the PCR-amplified fragment and increased the sensitivity of the PCR assay. Use of this probe enabled us to detect PCR-amplified product from B. malayi even when a product was not visible on an ethidium bromide-stained agarose gel. This increased sensitivity allowed us to detect the parasite in the heads of mosquitoes.
    Matched MeSH terms: Aedes/parasitology*; Insect Vectors/parasitology
  4. Ola-Fadunsin SD, Sharma RSK, Abdullah DA, Gimba FI, Jesse FFA, Sani RA
    Prev Vet Med, 2020 Jul;180:105027.
    PMID: 32442824 DOI: 10.1016/j.prevetmed.2020.105027
    There is need to confirm the presence of Theileria orientalis among the cattle population in Peninsular Malaysia and to evaluate the risk factors associated with the infection. To this effect, blood samples were collected from 1045 cattle from 43 farms throughout the entire States of Peninsular Malaysia. The collected blood samples were subjected to DNA extraction and subsequent PCR amplification of the major piroplasm surface protein (MPSP) gene of the haemoprotozoan. Representative positive amplicons were purified, sequenced and compared with other sequences of the MPSP gene of T. orientalis curated from the GenBank. A well-structured questionnaire was used to get information about each cattle, it's demography, the bio-security, environmental and management factors. Univariate and multivariate analysis were used for the statistical evaluation, with significance set at p < 0.05. A total prevalence of 49.76% (520/1045; 95% CI: 46.73 - 52.79) was obtained. Types of breeds, age, production type, herd size, level of farm biosecurity, farm size, presence of other animal species in the farm, management systems and prophylaxis were significantly (p < 0.05) associated with the prevalence of T. orientalis. This study confirmed the presence of T. orientalis and establish that the haemoprotozoan is endemic among cattle in Peninsular Malaysia.
    Matched MeSH terms: Cattle Diseases/parasitology; Theileriasis/parasitology
  5. Ahmad Loti NN, Mohd Noor MR, Chang SW
    J Sci Food Agric, 2021 Jul;101(9):3582-3594.
    PMID: 33275806 DOI: 10.1002/jsfa.10987
    BACKGROUND: Chili is one of the most important and high-value vegetable crops worldwide. However, pest and disease infections are among the main limiting factors in chili cultivation. These diseases cannot be eradicated but can be handled and monitored to mitigate the damage. Hence, the use of an automated identification system based on images will promote quick identification of chili disease. The features extracted from the images are of utmost importance to develop such an accurate identification system.

    RESULTS: In this research, chili pest and disease features extracted using the traditional approach were compared with features extracted using a deep-learning-based approach. A total of 974 chili leaf images were collected, which consisted of five types of diseases, two types of pest infestations, and a healthy type. Six traditional feature-based approaches and six deep-learning feature-based approaches were used to extract significant pests and disease features from the chili leaf images. The extracted features were fed into three machine learning classifiers, namely a support vector machine (SVM), a random forest (RF), and an artificial neural network (ANN) for the identification task. The results showed that deep learning feature-based approaches performed better than the traditional feature-based approaches. The best accuracy of 92.10% was obtained with the SVM classifier.

    CONCLUSION: A deep-learning feature-based approach could capture the details and characteristics between different types of chili pests and diseases even though they possessed similar visual patterns and symptoms. © 2020 Society of Chemical Industry.

    Matched MeSH terms: Plant Diseases/parasitology*; Plant Leaves/parasitology
  6. Lee HL, Krishnasamy M, Jeffery J
    Trop Biomed, 2005 Jun;22(1):87-8.
    PMID: 16880761
    Matched MeSH terms: Myiasis/parasitology; Nasopharyngeal Diseases/parasitology
  7. Morales-Figueroa GG, Castro-García M, Esparza-Romero J, López-Mata MA, Quihui-Cota L
    Trop Biomed, 2019 Sep 01;36(3):718-725.
    PMID: 33597494
    Caborca is one of the most productive asparagus-growing regions in the state of Sonora in northwest Mexico, an area where some fresh fruits and vegetables are sold at unregulated open-air street markets. This is a cross-sectional study in which fifty bundles of asparagus for exportation, 50 bundles of sub-standard asparagus, and 50 bundles of asparagus from open-air markets were selected randomly and then subjected to Faust, Kinyoun and ELISA testing to detect intestinal parasites. Pearson's chi-square (χ2) and Student-NewmanKeuls tests were used to estimate differences among the sampling site groups (P < 0.05). The pathogens Cryptosporidium spp. (29%) G. intestinalis (5%) and Cyclospora spp. (3%) were found in the asparagus sold in the region. The prevalence of Cryptosporidium spp. was higher in both the sub-standard asparagus and the product sampled from the open-air markets than in the samples for exportation (P < 0.05). This is the first study to demonstrate contamination by intestinal parasites in asparagus sold in different markets in northwest Mexico.
    Matched MeSH terms: Vegetables/parasitology*; Asparagus Plant/parasitology*
  8. Azrizal-Wahid N, Sofian-Azirun M, Low VL
    Trop Biomed, 2019 Dec 01;36(4):810-821.
    PMID: 33597453
    Fleas are the common cause of skin disorders in cats. They are well-known for transmitting various pathogens to both cats and humans. Accordingly, this study was conducted to gain insights on the risk factors associated with flea infestation on cats. Flea combing conducted on 426 cats from four distinct regions in Peninsular Malaysia revealed a relatively high rate of flea infestation on 306 cats (71.83%). A total of 651 fleas were collected, all of them were identified as Ctenocephalides felis with the total intensity of 2.13 and abundance of 1.53. The sex ratio of fleas was female-biased at 2.5:1 (♀=464, ♂=187). Statistical analysis of the data revealed that flea infestation was significantly (P<0.05) associated with several risk factors including region, age, weight, status (stray, sheltered, pet), body condition, and hair length. Higher flea prevalence was also observed in female cats (77.99%), big-sized cats (91.76%), stray cats (84.94%), cats with clean body condition (73.35%), and cats with long hairs (78.38%) as compared to their contemporaries within the same comparison variables. The high infestation of fleas in this study is indicative of cats as a flea reservoir particularly C. felis. Thus the findings of this study and the knowledge gained on the risk factors can be used to develop and improve control measures and management of flea infestations.
    Matched MeSH terms: Cat Diseases/parasitology*; Cats/parasitology*
  9. Quentin JC, Krishnasamy M, Tcheprakoff R
    Ann Parasitol Hum Comp, 1977 3 1;52(2):159-70.
    PMID: 907289
    Tarsubulura perarmata (Ratzel, 1868) is described from a primate Tarsius bancanus and from Tupaidae: Tupaia glis and T. minor in Malaysia (Kuala Lumpur). Its biological cycle is done by the experimental infestation of crickets belonging to the genera Valanga and Oxya. The infective larvae are obtained after three weeks of development of 28 degrees C in the intermediate host. They differ from third stage larvae obtained from Subulurinae by the development of cuticular pharyngeal lobes. The early apparition of this ontogenetic character confirms the isolation of the genus Tarsubulura as compared to the general evolution of the Subuluridae.
    Matched MeSH terms: Orthoptera/parasitology; Primates/parasitology*
  10. Petrányi G, Mieth H, Leitner I
    PMID: 1221502
    Infective larvae of Brugia malayi subperiodic obtained by dissection of infected Aedes togoi were injected subcutaneously into the scrotal region of Mastomys natalensis. From altogether 58 infected male M. natalensis 81% showed consistently or intermittently detectable microfilaraemia, whereas in 19% of the animals no microfilaraemia could be detected at any stage. The mean prepatent period was 136 days; the microfilarial density varied from 1 to 535 per 20 c. mm blood. In those animlas with consistently detectable and in general higher microfilaraemia an average of 13.1 live adult worms were found, against an average of 6.4 adult worms in animals with intermittent detectable and in general lower microfilaraemia. An average of 1.5 worms was found in animals which at no stage showed detectable microfilaraemia. A correlation between worm burden and prepatent period could be observed in the individual groups. From the total of 520 live adult worms recovered at necropsy, 37% were found in the lungs, 29% in the parenchyma of the testes and 34% in the lymphatic system. 47% of live fertile female worms were found in the lymphatic system, whereas the majority, i.e; 52% of infertile female worms were detected in the lungs. In addition, 380 encapsulated dead worms were found, most of them (98%) in the lymphatic system. 61% of a total of 900 live and dead worms were found in the region of the lymphatic system.
    Matched MeSH terms: Blood/parasitology; Lymphatic System/parasitology
  11. Chabaud AG, Krishnasamy M
    Ann Parasitol Hum Comp, 1975 Nov-Dec;50(6):813-20.
    PMID: 1227382
    Description of Trichospirura willmottae n. sp. parasite of the salivary ducts of Tupaia glis and T. sp. (single virgin female) parasite of the intestine of Myotis mystacinus in Malaysia. The two species are very closely related to the type species, a parasite of the pancreatic ducts of brasilian Primates, and can be differentiated mainly by the mensurations of the posterior extremities of the bodies. While the genus Rhabdochoma, parasite of the intestine of fresh-water fishes, underwent a very similar, but more or less pronounced, morphological evolution, it became adapted to many different hosts: Sea-fishes, Saurians, Mammals and to many locations. This evolutionary line includes six genera; Trichospirura, the only parasite in Mammals, is one of the more evolved. Some remarks are made on the host-distribution of Trichospirura, on the relationships between Rabdochonidae and Cystidicolidae and on the osmo-excretory apparatus of Trichospirura. The hypertrophy of this apparatus, which could be the consequence of the passage during the course of evolution from aquatic to terrestrial life, is comparable to that of the Pneumospirurinae.
    Matched MeSH terms: Chiroptera/parasitology; Tupaiidae/parasitology
  12. Quentin JC, Krishnasamy M
    Ann Parasitol Hum Comp, 1975 Nov-Dec;50(6):795-812.
    PMID: 818936
    Morphological study of two Spirura parasites of the oesophageal and the gastric wall of Tupaia and Nycticebus in Malaysia. -- Spirura malayensis n. sp. is found both in Tupaia in the District of Selangor (West Malaysia) and in Nycticebus coucang in Borneo. Its very primitive characteristics relate it to S. diplocyphos Chabaud, Brygoo and Petter, 1965, parasite of lemurs from Madagascar. Its larval development was obtained experimentally in Blatella germanica. -- Spirura aurangabadensis (Ali and Lovekar, 1966) described from a microchiroptera in India is found in west Malaysia in a Nycticebus coucang, and in a Tupaia glis. -- The distribution of the different species and the comparative study of the larval and adult cephalic structures show that the genus Spirura arose and became diversified in the old world in very primitive hosts according to two main evolutive lines.
    Matched MeSH terms: Strepsirhini/parasitology*; Tupaiidae/parasitology*
  13. Else JG, Colley FC
    J. Protozool., 1975 Nov;22(4):455-7.
    PMID: 1195155
    Oocysts and endogenous stages of new species of Eimeria and Isospora from the house lizard, Gehyra mutilata, are described. The ellipsoid to subspherical 2-layered oocysts of E. cicaki averaged 24.0 X 21.0 mum. Polar granules are present. Micropyle and oocyst residuum are absent. Ellipsoid sporocysts average 12.2 X 9.0 mum. A sporocyst residuum is present, but the Stieda body is absent. Endogenous stages are in epithelial cells of the small intestine. The subspherical single-layered oocysts of I. thavari average 23.8 X 22.8 mum. The polar granule is present; micropyle and oocyst residuum are absent. Ellipsoid sporocysts average 12.8 X 9.4 mum. Stieda body and sporocyst residuum are present. There are endogenous stages in epithelial cells of the small intestine.
    Matched MeSH terms: Feces/parasitology; Lizards/parasitology*
  14. Chong CH, Dissanaike AS, Fernando MA
    PMID: 4215148
    Matched MeSH terms: Birds/parasitology*; Blood/parasitology
  15. Dissanaike AS, Ong HT, Kan SP
    Trans R Soc Trop Med Hyg, 1974;68(6):494-5.
    PMID: 4460313
    Matched MeSH terms: Blood/parasitology; Lymphocytes/parasitology
  16. Quentin JC
    Ann Parasitol Hum Comp, 1977 9 1;52(5):559-67.
    PMID: 564154
    Description of a new species of Oxyurid: Syphacia (Syphatineria) callosciuri n. sp. parasite of a Sciurid rodent Callosciuris caniceps in Malaysia. In rodents belonging to the genus Callosciurus in Malaysia two species: S callosciuri n. sp. and S. owyangi Quentin, 1975, show morphological characteristics which are intermediate between the primitive Syphacia and two different lines of species parasite of recent rodents. These observations appear to indicate that the adaptation of Syphacia of Sciurids to the modern rodents has occured in South-East Asia.
    Matched MeSH terms: Sciuridae/parasitology*; Rats/parasitology*
  17. Ekawasti F, Kitagawa K, Domae H, Wardhana AH, Shibahara T, Uni S, et al.
    Parasitol Res, 2020 Apr;119(4):1271-1279.
    PMID: 32072327 DOI: 10.1007/s00436-020-06618-2
    To date, more than 50 Eimeria spp. have been isolated from marsupials of the family Macropodidae. Although 18 species of Eimeria have been previously detected from multiple animal species belonging to the genus Macropus of the family, limited genetic analyses of the parasites are available, and their pathogenicity remains unclear. Here, we report the isolation of Eimeria spp. from a zoo specimen of red-necked wallaby (Macropodidae; Macropus rufogriseus). Specifically, two distinct types of Eimeria oocysts were recovered, one from the feces before treatment with an anthelmintic and the second from the intestinal contents after death of the animal. The oocysts obtained from the two sources were morphologically identified as E. hestermani and E. prionotemni, respectively. We successfully determined partial gene sequences from the two isolates, including segments of the 18S rRNA genes, and for the first time have used phylogenetic analyses of these sequences to assign the species to distinct clades. In combination with further genetic data, these results are expected to help elucidate the pathogenicity and host ranges of Eimeria spp. within the respective family and genus.
    Matched MeSH terms: Feces/parasitology; Macropodidae/parasitology*
  18. Islam S, Rahman MK, Ferdous J, Rahman M, Akter S, Faraque MO, et al.
    Trop Biomed, 2020 Dec 01;37(4):842-851.
    PMID: 33612737 DOI: 10.47665/tb.37.4.842
    Hemoprotozoans are important pathogens of animals and humans, among which some species have zoonotic significance. The prevalence of different hemoprotozoa and Anaplasma spp. in larger mammals have been reported from different regions of the world. But, very few studies have been conducted to estimate the prevalence of hemoprotozoa in rodents and shrews of South-East Asia. The study assessed the prevalence of hemoprotozoa and Anaplasma spp. in rodents and shrews of Bangladesh. Blood samples (n=451) were collected from rodents and shrews between June 2011 and June 2013 and July-December 2015 from 4 land gradients of Bangladesh. Giemsa-stained blood smears revealed that 13% of animals were harboring hemoprotozoa (4.7% Babesia spp., 0.67% Plasmodium spp.), and Anaplasma spp. (7.5%). The study may serve as a guide for future hemoparasitic research of rodents and shrews.
    Matched MeSH terms: Rodentia/parasitology*; Shrews/parasitology*
  19. Zamri-Saad M, Subramaniam P, Sheikh-Omar AR, Sani RA, Rasedee A
    Vet Res Commun, 1994;18(2):119-22.
    PMID: 7975196
    Matched MeSH terms: Haemonchiasis/parasitology; Goat Diseases/parasitology*
  20. Sulaiman S, Sohadi AR, Yunus H, Iberahim R
    Med Vet Entomol, 1988 Jan;2(1):1-6.
    PMID: 2980156
    The role of some adult flies (Diptera: Cyclorrhapha) as carriers of helminth parasites of man was studied at four sites in Malaysia: a refuse dump, where no helminth-positive flies were detected, and in three peri-domestic situations where four species of flies carried up to three types of nematodes. The dominant fly species Chrysomya megacephala (Fabricius) carried eggs of the roundworm Ascaris lumbricoides L., the pinworm Trichuris trichiura (L.) and hookworm on the adult external body surface and in the gut lumen, in association with Bukit Lanjan aborigines. Chrysomya rufifacies (Macquart) and Sarcophaga spp. also had Ascaris lumbricoides and Trichurus trichiura eggs in their gut contents. Human helminths were not recovered from Lispe leucospila (Wiedemann), Lucilia cuprina (Wiedemann) or the housefly Musca domestica L. In an urban slum area of Kuala Lumpur city, filariform larvae identified as the hookworm Necator americanus (Stiles) occurred in the intestines of the face-fly Musca sorbens Wiedemann (22 larvae per 100 flies) and of Chrysomya megacephala (4.5 larvae per 100 flies). This concentration of apparently infective N. americanus in M. sorbens, a fly which often breeds in faeces and browses on human skin, could have transmission potential.
    Matched MeSH terms: Diptera/parasitology*; Insect Vectors/parasitology*
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