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  1. Marchette NJ, Garcia R, Rudnick A
    Am J Trop Med Hyg, 1969 May;18(3):411-5.
    PMID: 4976739
    Matched MeSH terms: Zika Virus/isolation & purification*
  2. Van Hong N, van den Eede P, Van Overmeir C, Vythilingham I, Rosanas-Urgell A, Vinh Thanh P, et al.
    Am J Trop Med Hyg, 2013 Oct;89(4):721-3.
    PMID: 23980132 DOI: 10.4269/ajtmh.13-0027
    We have modified an existing semi-nested multiplex polymerase chain reaction (PCR) by adding one Plasmodium knowlesi-specific nested PCR, and validated the latter against laboratory and clinical samples. This new method has the advantage of being relatively affordable in low resource settings while identifying the five human Plasmodium species with a three-step PCR.
    Matched MeSH terms: Plasmodium/isolation & purification*
  3. Lambros C, Davis DR, Lewis GE
    Am J Trop Med Hyg, 1989 Jul;41(1):3-8.
    PMID: 2669543 DOI: 10.4269/ajtmh.1989.41.1.TM0410010003
    The drug susceptibility of 70 isolates of Plasmodium falciparum to standard and experimental antimalarials was evaluated using a radioisotope microdilution method. All isolates were from forest fringe dwelling Orang Asli, the aborigines of Peninsular Malaysia. The geometric mean IC50 values were: chloroquine, 10 ng/ml; amodiaquine, 4.7 ng/ml; mefloquine, 2.8 ng/ml; quinine, 40.5 ng/ml; halofantrine, 1.5 ng/ml; enpiroline, 3 ng/ml; and pyrimethamine, 21 ng/ml. Four isolates exhibited decreased susceptibility to chloroquine (IC50 greater than 60 ng/ml), and one exhibited decreased susceptibility to quinine (IC50 = 161 ng/ml). Three isolates showed decreased susceptibility to mefloquine (IC50 = 10-11 ng/ml). The lack of drug pressure may account for the high prevalence of P. falciparum isolates susceptible to chloroquine.
    Matched MeSH terms: Plasmodium falciparum/isolation & purification
  4. Singh B, Bobogare A, Cox-Singh J, Snounou G, Abdullah MS, Rahman HA
    Am J Trop Med Hyg, 1999 Apr;60(4):687-92.
    PMID: 10348249
    A nested polymerase chain reaction (PCR) assay that uses Plasmodium genus-specific primers for the initial PCR (nest 1) amplification and either genus- or species-specific primers for the nest 2 amplifications was tested on laboratory and field samples. With in vitro cultured Plasmodium falciparum-infected blood samples, it was capable of detecting six parasites/microl of blood using DNA prepared from 25-microl blood spots on filter paper. The assay was evaluated on fingerprick blood samples collected on filter paper from 129 individuals living in a malaria-endemic area in Malaysia. Malaria prevalence by genus-specific nested PCR was 35.6% (46 of 129) compared with 28.7% (37 of 129) by microscopy. The nested PCR detected seven more malaria samples than microscopy in the first round of microscopic examination, malaria in three microscopically negative samples, six double infections identified as single infections by microscopy and one triple infection identified as a double infection by microscopy. The nested PCR assay described is a sensitive technique for collecting accurate malaria epidemiologic data. When coupled with simple blood spot sampling, it is particularly useful for screening communities in remote regions of the world.
    Matched MeSH terms: Plasmodium falciparum/isolation & purification*
  5. Ayu SM, Lai LR, Chan YF, Hatim A, Hairi NN, Ayob A, et al.
    Am J Trop Med Hyg, 2010 Dec;83(6):1245-8.
    PMID: 21118929 DOI: 10.4269/ajtmh.2010.10-0279
    In 2006, an outbreak of Chikungunya virus (CHIKV) of the Asian genotype affected over 200 people in Bagan Panchor village in Malaysia. One year later, a post-outbreak survey was performed to determine attack rate, asymptomatic rate, and post-infection sequelae. Findings were compared with recent CHIKV outbreaks of the Central/East African genotype. A total of 180 residents were interviewed for acute symptoms and post-infection physical quality of life and depressive symptoms. Sera from 72 residents were tested for CHIKV neutralizing antibodies. The estimated attack rate was 55.6%, and 17.5% of infected residents were asymptomatic. Arthralgia was reported up to 3 months after infection, but there were no reports of long-term functional dependence or depression. Symptomatic and seropositive residents were significantly more likely to live in the area with the most dense housing and commercial activities. CHIKV had a high attack rate and considerable clinical impact during the Bagan Panchor outbreak.
    Matched MeSH terms: Chikungunya virus/isolation & purification*
  6. Chong Sue Kheng, Teoh Kim Chee, Marchette NJ, Garcia R, Rudnick A, Coughlan RF
    Aust Vet J, 1968 Jan;44(1):23-5.
    PMID: 5689238
    Matched MeSH terms: Encephalitis Viruses/isolation & purification
  7. Hussain A, Gorsi FI, Ali MQ, Yaqub S, Asif A, Bibi B, et al.
    Food Chem, 2025 Feb 15;465(Pt 2):142129.
    PMID: 39579399 DOI: 10.1016/j.foodchem.2024.142129
    Valorizing neglected crop byproducts through eco-friendly techniques has gained attention as underutilized crops offer new sources of bioactive components. This study examined the chemical composition, phytochemicals, and antioxidant activities in six fractions of the underutilized chayote (Sechium edule). Each fraction was microwave-dried and extracted with ultrasound assistance. Shoots were high in ash and fiber, while pulp had high moisture and carbohydrates, and seeds were rich in fat and protein. Shoots, leaves, roots, and peels had high mineral levels, particularly Ca, K, Mg, P, Fe, and Zn. Shoots were also high in phenolics, flavonoids, and ascorbic acid, while leaves had a high carotenoid content. Polyphenol compounds such as myricetin and catechin were abundant in shoots, which exhibited the highest antioxidant activity. Chayote shoots, roots, and leaves are abundant in bioactive compounds that can be utilized in food and, therefore, offers an opportunity for utilization of a currently neglected plant byproduct.
    Matched MeSH terms: Phytochemicals/isolation & purification
  8. Li X, Tiang MF, Cui X, Li Y, Wang Z, Zhao L, et al.
    Int J Biol Macromol, 2024 Dec;283(Pt 4):137989.
    PMID: 39581417 DOI: 10.1016/j.ijbiomac.2024.137989
    In this pioneering study, electrostatic spraying (ES) technology with high voltages is proposed to reduce the size of hydrogel microbeads further, aiming to enhance the adsorption rate of cationic methylene blue (MB) dye. The increased voltages, ranging from 0.0 to 13.0 kV, further decreased the size of electrostatically sprayed hydrogel microbeads crosslinked by hydrogen bonds between sodium alginate (SA) and carboxymethyl chitosan (CMCS) in hydrochloric acid. The size of SA/CMCS hydrogel microbeads was successfully reduced from 2000 ± 121 μm (SC-2000) to 400 ± 15 μm (SC-400). Notably, SC-400 exhibits the highest maximum adsorption capacity (qm) and rate constant (k2) at 840.3 mg/g and 0.0598 g/mg/min, respectively, at pH 9.0 and a temperature of 25 °C in the absence of ionic compounds, which is three times higher than that of SC-2000, due to their high specific surface area and pore volume. Through a series of adsorption studies and characterization analyses, SA/CMCS hydrogel microbeads displayed heterogeneous adsorption behaviors towards MB dye through electrostatic interactions between the deprotonated carboxylic groups and cationic MB molecules, where MB adsorption efficiency could be significantly influenced by pH and ionic strength. These findings suggest that ES technology is effective in synthesizing smaller SA/CMCS hydrogel microbeads with enhanced MB removal rates and stable adsorption capacities and their applications could be further explored for removing other organic dyes and toxic metals in subsequent research studies.
    Matched MeSH terms: Water Pollutants, Chemical/isolation & purification
  9. Murdiyarso LS, Rajahram GS, Tan AF, Piera KA, William T, Oyong DA, et al.
    Am J Trop Med Hyg, 2025 Jan 08;112(1):85-88.
    PMID: 39531730 DOI: 10.4269/ajtmh.24-0264
    Zoonotic malaria presents a major public health challenge in Southeast Asia. Plasmodium cynomolgi coinfects the same macaque hosts and mosquito vectors as the most common cause of zoonotic malaria, Plasmodium knowlesi. Plasmodium cynomolgi appears morphologically similar to Plasmodium vivax on microscopy and can amplify P. vivax polymerase chain reaction (PCR) assays, confounding transmission estimates. We screened 2,103 samples for P. cynomolgi across all 26 districts in Sabah, Malaysia, from 2010 to 2021. Samples comprised 1,425 P. knowlesi, 256 P. vivax, 293 P. falciparum, and 31 Plasmodium malariae PCR-confirmed malaria cases and 100 malaria microscopy-positive and species-specific PCR-negative samples. A nested PCR assay targeting P. cynomolgi-specific 18S small subunit ribosomal ribonucleic acid with a detection limit of ∼2 parasites/µL was conducted on whole blood samples. No P. cynomolgi infections were detected. Symptomatic P. cynomolgi co-infections appear rare in Malaysia, although prevalence may be underestimated owing to the absence of routine molecular screening and the sensitivity of available assays.
    Matched MeSH terms: Plasmodium knowlesi/isolation & purification
  10. Jawad AH, Maharani RA, Hapiz A, Khadiran T, Jani NA, ALOthman ZA, et al.
    Int J Biol Macromol, 2025 Jan;286:138373.
    PMID: 39643197 DOI: 10.1016/j.ijbiomac.2024.138373
    Herein, a mesoporous magnetic chitosan-salicylaldehyde/calcium oxide nanoparticle (CS-SL/CaO/Fe3O4) biocomposite adsorbent that was prepared via freeze-drying. The CS-SL/CaO/Fe3O4 was utilized for the adsorption of ramazol brilliant blue (RBB) dye from aqueous solution. The physicochemical properties of the CS-SL/CaO/Fe3O4 were evaluated using diverse characterization techniques, including BET, XRD, FTIR, FESEM-EDX, CHNS, and pHpzc. The three main factors for adsorption included the following A: CS-SL/CaO/Fe3O4 dosage (0.02-0.1 g/100 mL), B: pH (4-10), and C: Time (60-540 min). These factors were improved using statistical methods, specifically the Box-Behnken design (BBD). The optimal conditions for achieving maximum RBB removal (62.5 %) are listed: CS-SL/CaO/Fe3O4 dosage of 0.1 g/100 mL, a solution pH of 7, and a contact time of 540 min. The adsorption kinetics and equilibrium isotherms were well described by the pseudo first order (PFO) kinetic and Langmuir isotherm models, respectively. Thus, the CS-SL/CaO/Fe3O4 material has a maximum adsorption capacity (qmax) of 63.3 mg/g for RBB at 25 °C. The adsorption mechanism of RBB onto the CS-SL/CaO/Fe3O4 surface was attributed to electrostatic forces, n-π stacking, H-bonding, and Pi-Pi interactions. Thus, CS-SL/CaO/Fe3O4 represents a recoverable magnetic adsorbent with potential for capture of organic dyes from wastewater.
    Matched MeSH terms: Coloring Agents/isolation & purification
  11. Saha S, Chadha M, Al Mamun A, Rahman M, Sturm-Ramirez K, Chittaganpitch M, et al.
    Bull World Health Organ, 2014 May 01;92(5):318-30.
    PMID: 24839321 DOI: 10.2471/BLT.13.124412
    OBJECTIVE: To characterize influenza seasonality and identify the best time of the year for vaccination against influenza in tropical and subtropical countries of southern and south-eastern Asia that lie north of the equator.

    METHODS: Weekly influenza surveillance data for 2006 to 2011 were obtained from Bangladesh, Cambodia, India, Indonesia, the Lao People's Democratic Republic, Malaysia, the Philippines, Singapore, Thailand and Viet Nam. Weekly rates of influenza activity were based on the percentage of all nasopharyngeal samples collected during the year that tested positive for influenza virus or viral nucleic acid on any given week. Monthly positivity rates were then calculated to define annual peaks of influenza activity in each country and across countries.

    FINDINGS: Influenza activity peaked between June/July and October in seven countries, three of which showed a second peak in December to February. Countries closer to the equator had year-round circulation without discrete peaks. Viral types and subtypes varied from year to year but not across countries in a given year. The cumulative proportion of specimens that tested positive from June to November was > 60% in Bangladesh, Cambodia, India, the Lao People's Democratic Republic, the Philippines, Thailand and Viet Nam. Thus, these tropical and subtropical countries exhibited earlier influenza activity peaks than temperate climate countries north of the equator.

    CONCLUSION: Most southern and south-eastern Asian countries lying north of the equator should consider vaccinating against influenza from April to June; countries near the equator without a distinct peak in influenza activity can base vaccination timing on local factors.

    Matched MeSH terms: Orthomyxoviridae/isolation & purification*
  12. Mirkovic RR, Kono R, Yin-Murphy M, Sohier R, Schmidt NJ, Melnick JL
    Bull World Health Organ, 1973;49(4):341-6.
    PMID: 4368683
    A new enterovirus, now classified as enterovirus type 70, was isolated from the conjunctiva of patients with acute haemorrhagic conjunctivitis during the 1971 epidemics that occurred in Japan, Singapore, and Morocco. These epidemics were parts of a pandemic involving Africa (Algeria, Ghana, Morocco, Nigeria, and Tunisia), Asia (Cambodia, China (Province of Taiwan), Hong Kong, India, Indonesia, Japan, Malaysia, the Philippines, Singapore, and Thailand), and England during 1969-71. A representative strain from each of the three epidemic areas was studied cooperatively. The strains exhibited the physicochemical characteristics of enteroviruses. Cross-neutralization tests showed that these viruses were distinct from all known human enterovirus immunotypes, but that they were antigenically closely related. The human origin of the viruses was demonstrated by the appearance of homologous neutralizing antibodies during convalescence in patients with acute haemorrhagic conjunctivitis.
    Matched MeSH terms: Enterovirus/isolation & purification*
  13. Collins WE, Warren M, Skinner JC, Fredericks HJ
    Bull World Health Organ, 1968;39(3):451-63.
    PMID: 4882987
    The fluorescent antibody (FA) technique was used to detect the presence of malarial antibody in populations living in 3 different ecological areas of Malaysia. Serum samples were tested using Plasmodium falciparum, P. vivax, P. malariae and P. fieldi antigens. An area of hyperendemic malaria had a good correlation between the antibody responses and active parasitaemias. The percentage and intensity of responses increased with the age of the individuals. In an area of hypoendemic malaria, each of 17 sites had ecological conditions which would favour or discourage the transmission of malaria. The reasons for high FA responses in some villages and low responses in others were readily apparent. The effect of even limited control programmes on the malarial ecology could be measured by an examination of the antibody responses. An aboriginal population receiving suppressive drugs had FA responses indicating both past experience and the effect of the drug programme.
    Matched MeSH terms: Plasmodium/isolation & purification*
  14. Bermawi B, Kurniasari DW, Imaniar A, Hidayatih N, Lisnawati DA, Rofananda IF, et al.
    Med J Malaysia, 2024 Sep;79(5):604-607.
    PMID: 39352164
    INTRODUCTION: Tuberculosis (TB) is a serious global health problem in Indonesia, which is the country with the secondhighest TB burden after India. Accuracy in TB diagnosis is the key to effective treatment and decreased transmission rate. One of the latest diagnostic methods is interferon gamma release assay (IGRA), which measures the interferon-γ release associated with Mycobacterium tuberculosis (MTB) infection. This study aims to determine the diagnostic value of IGRA-TB using IchromaTM IGRA-TB diagnostic kit (sensitivity, specificity, positive predictive value [PPV], and negative predictive value [NPV]), compared to Ziehl-Neelsen (AFB) staining, nucleic acid amplificationbased test (Xpert-MTB) and chest-X Ray as the gold standard in TB diagnosis.

    MATERIALS AND METHODS: A cross-sectional observational study design was used. Patients were recruited through purposive sampling from pulmonology outpatient clinic and inpatient ward at Jemursari Islamic Hospital (RSI Jemursari), Surabaya from July 2023 to December 2023. All enrolled patients should have been previously tested positive or negative for pulmonary TB using AFB staining, Xpert MTB and chest x-ray. Blood samples of the patients were collected and processed using the IchromaTM IGRA-TB diagnostic kit. The results were then compared with gold standard methods for calculating the IGRA-TB diagnostic value.

    RESULTS: A total of 56 adult patients were enrolled in this study. The sensitivity, specificity, PPV, NPV and accuracy rate of IGRA-TB using IchromaTM IGRA-TB diagnostic kit were 80.56%, 85%, 90.62%, 70.83% and 82.14%, respectively.

    CONCLUSION: IchromaTM IGRA-TB showed reasonably high diagnostic sensitivity and specificity, indicating that this method can be further utilised as a diagnostic and screening tool for pulmonary TB.

    Matched MeSH terms: Mycobacterium tuberculosis/isolation & purification
  15. Amer MM, Soliman AM, Do T, Hang L, El-Sayed SAE, Jaroszewski J, et al.
    Trop Biomed, 2024 Dec 01;41(4):412-421.
    PMID: 39876497 DOI: 10.47665/tb.41.S.005
    In Egypt, knowledge about vector-borne bacterial pathogens in camels remains limited. To address this gap, 181 blood samples from adult one-humped camels (Camelus dromedarius) in the greater Cairo metropolitan area were collected from October 2021 to March 2022. Through PCR assays, four pathogens were detected, where Anaplasmataceae being the most common (54.7%), followed by hemotropic Mycoplasma spp. (29.3%), Rickettsia spp. (12.2%), and Coxiella burnetii (1.7%). Comparative sequence analysis revealed novel findings, including: 1) the identification of two distinct hemotropic Mycoplasma spp., one closely related to bovine Mycoplasma sp. (Mycoplasma wenyonii), and the other closely related to porcine Mycoplasma sp. (Candidatus Mycoplasma haemosuis); and 2) the detection of Anaplasma bovis and Anaplasma phagocytophilum. Additionally, Anaplasma platys, Rickettsia africae, and Coxiella burnetii were identified as well. It's worth noting that these vector-borne pathogens possess zoonotic potential, emphasizing the need for adopting a "One Health" approach in Egypt to safeguard the wellbeing of both humans and animals.
    Matched MeSH terms: Anaplasmataceae/isolation & purification
  16. Onukak AE, Nwagboso CI, Akpu BB, Etim AJ, Benjamin OE, Ereh SE, et al.
    J Mycol Med, 2024 Dec;34(4):101511.
    PMID: 39500230 DOI: 10.1016/j.mycmed.2024.101511
    Although classified as an AIDS-defining illness, several reports show histoplasmosis also affects patients living with cancers including haematological malignancies and solid tumours. However, reviews describing cases of histoplasmosis in malignancies are lacking in the literature. We identified a total of thirty-four cases with twenty (58.8 %) cases reported from the USA, four from Brazil (11.8 %), three from India (8.8 %), and one each from Singapore (2.9 %), France (2.9 %), Netherlands (2.9 %), Colombia (2.9 %), Canada (2.9 %), Morocco (2.9 %), and Malaysia (2.9 %). 82.4 % (n = 28) of the cases were adults. Presenting symptoms were majorly fever (61.7 %), lymphadenopathy (50.0 %) and weight loss (29.4 %). Essential haematologic findings were pancytopaenia (n = 7, 20.6 %), neutropenia (n = 2, 5.9 %) and anaemia (n = 5, 14.7 %). The associated cancers were predominantly haematological and comprised 73.5 % (n = 25) of all cases. The diagnosis of histoplasmosis was via histopathology (n = 23, 67.6%), culture (n = 13, 38.2%), Histoplasma antigen assay (n = 13, 38.2%), anti-Histoplasma antibody assay (n = 5, 14.7%), PCR and sequencing (n = 2, 5.9%), peripheral blood film/direct microscopy (n = 4, 11.8%) and cytology (n = 1, 2.9%). Of the thirty-four cases, twenty-four (70.6%) had favourable outcomes, eight (23.5%) died, one (2.9%) was lost to follow-up and in one (2.9%) case, the outcome was not stated. Histoplasmosis is not an uncommon opportunistic disease complicating malignancies but is paradoxically underdiagnosed in Africa given the huge burden of cancers in that region. Besides following chemotherapy and the use of steroids, tumour necrosis factor-α antagonists therapy, hematopoietic stem cell transplantation and environmental exposure were factors associated with Histoplasma infection in patients with malignancies. A resolution to promptly screen suspected or confirmed cases of malignancies for histoplasmosis will improve diagnosis and clinical outcomes.
    Matched MeSH terms: Histoplasma/isolation & purification
  17. Ngeow YF
    Ann Acad Med Singap, 1996 Mar;25(2):300-4.
    PMID: 8799029
    Infection with Chlamydia trachomatis results in the formation of a variety of antibodies with group, species, subspecies and serovarspecificity. Sera from patients with genital tract infections often show broad reactivity in serological tests. This may be due to the presence of cross-reacting antibodies, repeated infections by different serotypes or concurrent genital and respiratory infections by different chlamydial species. Other factors contributing to difficulties in interpretation include how antibody titres in acute mucosal infections, the occurrence of latent infections and reactivations, and the persistence of IgG which does not allow the differentiation of past from current infections. For these reasons, serology alone is inadequate for the diagnosis of uncomplicated lower genital tract infections. In upper genital tract infections, however, because of difficulties with sampling from the infected site, a positive serology may be the only indications of chlamydial involvement. This paper discusses the principles of chlamydial antibody assays, difficulties with their interpretation and their role in the diagnosis of upper and lower genital tract infections.
    Matched MeSH terms: Chlamydia trachomatis/isolation & purification
  18. Choo KE, Wan Ariffin WA, Chua SP
    Ann Acad Med Singap, 1988 Jul;17(3):438-42.
    PMID: 3218934
    A retrospective study of 84 cases of neonatal septicaemia admitted into a neonatal unit in a rural area of Malaysia for 1 year between 1st September 1985 to 31st August 1986 was carried out to determine the spectrum of micro-organisms and predisposing factors in relation to early and late onset septicaemia. The incidence of neonatal septicaemia was 2.13 per 1,000 live-births per year and the case fatality was 41.7% with higher case fatality in those who were premature, those who presented as early onset and those who had gram negative septicaemia. The mean age of onset of septicaemia was 7.8 days (range from 2 hours to 27 days). Forty four (52%) neonates had early onset septicaemia with mean age of onset at 2.7 days; forty (48%) neonates had late onset septicaemia presenting at 13.6 days of life. Gram negative organisms such as Klebsiella, Pseudomonas, sp., E. coli and Streptococcus, especially group B Streptococcus were the major organisms in the early onset septicaemia. Staphylococcus aureus and Staphylococcus epidermidis were the major organisms responsible for the late onset septicaemia. Obstetrical factors played an important role in early onset septicaemia. Prematurity was the most common predisposing factor. Invasive diagnostic and therapeutic procedures including surgery highlighted once again the importance of these procedures in predisposing the newborn to infection.
    Matched MeSH terms: Bacteria/isolation & purification
  19. Chan YC, Wong TW, Yap EH
    Ann Acad Med Singap, 1987 Oct;16(4):696-701.
    PMID: 2895603
    Haemorrhagic fever with renal syndrome (HFRS) is caused by a group of RNA viruses within the family of Bunyaviridae known as hantaviruses. The classical, severe form of HFRS is characterized by fever, headache, abdominal and lumbar pain, proteinuria, haemorrhagic phenomena, shock and renal failure. The disease is associated with the prototype Hantaan virus and occurs in rural areas of Korea and China with Apodemus mice as reservoir hosts. A clinically less severe form of HFRS, which is caused by Seoul virus, occurs in urban areas with the house rat Rattus novegicus as the main reservoir host. The disease in nonendemic areas may be atypical and patients with symptoms the hepatitis and minimal renal involvement have been observed in Malaysia. Outbreaks of HFRS in humans involving infected laboratory rat colonies have occurred in several medical centres in various countries. Hantaviruses cause a chronic, asymptomatic infection in rodents which excrete the virus in their lungs, saliva and urine. Man becomes infected mainly by inhalation of infected droplets from healthy rodent carriers. Seroepidemiological studies using mainly the indirect immunoflourescent antibody test of sera from humans and rats showed that hantaviruses have a worldwide distribution.
    Matched MeSH terms: Hantavirus/isolation & purification
  20. Suresh K, Smith HV, Tan TC
    Appl Environ Microbiol, 2005 Sep;71(9):5619-20.
    PMID: 16151162
    Blastocystis cysts were detected in 38% (47/123) (37 Scottish, 17 Malaysian) of sewage treatment works. Fifty percent of influents (29% Scottish, 76% Malaysian) and 28% of effluents (9% Scottish, 60% Malaysian) contained viable cysts. Viable cysts, discharged in effluent, provide further evidence for the potential for waterborne transmission of Blastocystis.
    Matched MeSH terms: Blastocystis/isolation & purification
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