A modified semi-nested multiplex malaria PCR (SnM-PCR) for the identification of the five human Plasmodium species occurring in Southeast Asia

Van Hong N; van den Eede P; Van Overmeir C; Vythilingham I; Rosanas-Urgell A; Vinh Thanh P; Thang ND; Hung NM; Hung le X; D'Alessandro U; Erhart A

doi:10.4269/ajtmh.13-0027

Fulltext

A modified semi-nested multiplex malaria PCR (SnM-PCR) for the identification of the five human Plasmodium species occurring in Southeast Asia

Van Hong N ¹ , van den Eede P , Van Overmeir C , Vythilingham I , Rosanas-Urgell A , Vinh Thanh P Show all authors , Thang ND , Hung NM , Hung le X , D'Alessandro U , Erhart A

Affiliations

¹ National Institute of Malariology, Parasitology and Entomology, Hanoi, Vietnam; Malariology Unit, Institute of Tropical Medicine, Antwerp, Belgium; Parasitology Department, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia; Parasitology Unit, Infectious Disease Research Centre, Institute for Medical Research (IMR), Jalan Pahang, Kuala Lumpur, Malaysia; Diseases Control and Elimination Theme, Medical Research Unit, Fajara, The Gambia

Am J Trop Med Hyg, 2013 Oct;89(4):721-3.

PMID: 23980132 DOI: 10.4269/ajtmh.13-0027

Abstract

We have modified an existing semi-nested multiplex polymerase chain reaction (PCR) by adding one Plasmodium knowlesi-specific nested PCR, and validated the latter against laboratory and clinical samples. This new method has the advantage of being relatively affordable in low resource settings while identifying the five human Plasmodium species with a three-step PCR.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.