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Analysis of 30 Biallelic INDEL Markers Using the Investigator DIPplex(®) Kit

Bashir M, Hassan NH

Methods Mol Biol, 2016;1420:135-42.
PMID: 27259737 DOI: 10.1007/978-1-4939-3597-0_11

Insertion/deletion polymorphisms (INDELs) are a relatively new class of a DNA marker to be used in forensic casework; used most commonly as a supplementary method to STR-based typing. INDELs, like SNPs, are particularly useful for the analysis of highly degraded DNA as the amplicon sizes are typically below 160 bp; they can also be valuable as an additional tool to help resolve kinship cases, with the advantage over STRs that they do not have high mutation rates. INDELs have an advantage over SNPs in that they are length polymorphisms and so can be analyzed by simply measuring the length of the allele(s). The Qiagen Investigator(®) DIPplex Kit is currently only one of two commercially available kits for the amplification of INDEL polymorphisms; it amplifies 30 biallelic INDEL loci and the amelogenin locus. The primers used are fluorescence labeled with 6-FAM, BTG, BTY, and BTR. This technique is robust, relatively simple, and the results are analyzed using the same capillary electrophoresis equipment and software as used for STR typing.
Fulltext Effects of high-intensity interval training on selected indicators of physical fitness among male team-sport athletes: A systematic review and meta-analysis

Yuan Y, Soh KG, Qi F, Bashir M, Zhao N

PLoS One, 2024;19(11):e0310955.
PMID: 39536016 DOI: 10.1371/journal.pone.0310955

BACKGROUND: Superior physical fitness and performance are essential in male team sports. Among a myriad of training methodologies, high-intensity interval training (HIIT) has gained popularity owing to its unparalleled efficiency and effectiveness. Previous studies have established that HIIT is a proven and effective approach for enhancing various physiological performance outcomes, particularly oxygen consumption capacity, in individual sports. Despite potential differences in training practices between male and female athletes, HIIT is recognized as an anaerobic training approach for team-sport athletes. This systematic review aimed to comprehensively and innovatively analyze the existing literature to examine the effectiveness of HIIT on oxygen consumption performance among male team-sport athletes.
METHODS: A comprehensive literature search was conducted in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines across the PubMed, SCOPUS, Web of Science, and SPORTDiscus databases until December 31, 2023. The inclusion criteria for this review encompassed research articles published in peer-reviewed journals that specifically focused on the impact of HIIT on the oxygen consumption performance of male players engaged in team sports. The study population exclusively consisted of male participants. The collected data included study characteristics, participant demographics, intervention details, and outcomes. Methodological quality assessment was performed using standardized criteria. The effect sizes (ESs) were calculated, and a meta-analysis was conducted using a random-effects model.
RESULTS: The literature search yielded 13 eligible studies encompassing 286 athletes aged 14-26 years. The meta-analysis showed statistically significant enhancements in maximal oxygen uptake (VO2max) in six studies (ES, 0.19-0.74; p < 0.005), Yo-Yo Intermittent Recovery Test (YYIRT) performance in six studies (ES, 0.20-2.07; p = 0.009), repeated-sprint ability total time (RSAtotal) in five studies (ES, 0.18-1.33; p < 0.001), and the best and average times for repeated-sprint ability (RSAbest and RSAmean, respectively) in four studies (ES, 0.47-1.50; p < 0.001). However, two studies did not report any significant differences in the outcomes of the Velocity in 30-15 Intermittent Fitness Test (VIFT) between the experimental and control groups (ES, -0.08 and -0.27; p = 0.87 and 0.443, respectively). Moreover, one study did not report any significant differences in the maximal aerobic speed (MAS) (ES, 0.41, p = 0.403).
CONCLUSIONS: HIIT significantly improved VO2max, YYIRT, and RSA; however, it did not appear to enhance VIFT and MAS performance, irrespective of age or competition level. These findings indicate that HIIT could serve as a valuable method for improving oxygen consumption performance (VO2max, YYIRT, and RSA) in male team-sport athletes, offering a time-efficient alternative to the traditional training methods. Further research is warranted to investigate its impact on performance outcomes in competitive settings and identify optimal HIIT protocols tailored to specific team sports.
Enhancing Physical Fitness in Elite Field Hockey Players with A Twelve-Week Functional Training Program: A Cluster Randomized Control Trial

Bashir M, Hucheng M, Geok SK, Akbar S, Zhang D

J Sports Sci Med, 2024 Sep;23(1):628-637.
PMID: 39228773 DOI: 10.52082/jssm.2024.628

This study aimed to explore the impact of functional training on the physical fitness of young elite field hockey players. The study comprised 40 young elite male field hockey players with the following characteristics (mean ± SD age: 21.5 ± 0.8 years; height: 176.9 ± 2.6 cm; weight: 68.4 ± 5.1 kg; BMI: 21.8 ± 1.3; training experience: 51.2 ± 5.4 months). Twenty participants were allocated to two groups: the functional training group (FTG) and the control training group (CG). Each group received 60-minute training sessions three times per week for 12 weeks. Generalized estimating equation analysis and a Bonferroni test for pairwise comparisons were used to assess the intervention's efficacy. Before the start of the exercise program, no statistically significant differences were observed in physical fitness measures between the FTG and CG (p > 0.05). However, by the sixth week, a significant difference appeared in both the T-Agility test (p < 0.001) and endurance (p = 0.024) between the two groups, while no notable distinctions were detected in other fitness parameters (p > 0.05). After a 12-week training program, the FTG demonstrated improvements in all physical fitness measures [flexibility (p < 0.001); Illinois agility test (p < 0.001); T-agility test (p = 0.020); endurance (p < 0.001)] except speed, which exhibited no significant impact (p = 0.175). Notable enhancements in T-agility and endurance were evident after just six weeks of functional training, and a 12-week functional training regimen showed superiority over standard training approaches in young elite male field hockey players. These findings encourage the efficacy of functional training exercises over traditional methods in enhancing athletes' fitness parameters.
Current status in decision making to treat acute type A dissection: limited versus extended repair

Velayudhan BV, Idhrees M, Matalanis G, Park KH, Tang D, Sfeir PM, et al.

J Cardiovasc Surg (Torino), 2020 Jun;61(3):285-291.
PMID: 32337940 DOI: 10.23736/S0021-9509.20.11397-1

Acute type A aortic dissection remains one of the most challenging conditions in aortic surgery. Despite the advancements in the field, the mortality rate still remains high. Though there is a general consensus that the ascending aorta should be replaced, the distal extension of the surgery still remains a controversy. Few surgeons argue for a conservative approach to reduce operative and postoperative morbidity while others considering the problems associated with "downstream problems" support an aggressive approach including a frozen elephant trunk. The cohort in the Indian subcontinent and APAC is far different from the western world. Many factors determine the decision for surgery apart from the pathology of the disease. Economy, availability of the suitable prosthesis, the experience of the surgeon, ease of access to the medical facility all contribute to the decision making to treat acute type A dissection.
Corrigendum to 'Unpacking the environmental quality through the effects of natural resources, renewable energy consumption, banking development and industrial value addition: An empirical evidence from BRICS countries' [J. Environ. Manage. 367, September 2024, 122058]

Adnan Bashir M, Qing L, Dewil R, Xi Z, Razi U, Jingting L

J Environ Manage, 2024 Aug 16.
PMID: 39153948 DOI: 10.1016/j.jenvman.2024.122221
Effects of Different Exercise Training on Physical Fitness and Technical Skills in Handball Players. A Systematic Review

Akbar S, Kim Geok S, Bashir M, Jazaily Bin Mohd NN, Luo S, He S

J Strength Cond Res, 2024 Aug 14.
PMID: 39172757 DOI: 10.1519/JSC.0000000000004908

Akbar, S, Kim Geok, S, Bashir, M, Jazaily Bin Mohd, NN, Luo, S, and He, S. Effects of different exercise training on physical fitness and technical skills in handball players. A systematic review. J Strength Cond Res XX(X): 000-000, 2024-This review aimed to assess the effects of exercise training on handball players' performance. A thorough search was conducted in 5 online databases (ProQuest, PubMed, Science Direct, Web of Science, and EBSCOhost (SPORTDiscus), as well as on Google Scholar and other gray literature references starting on April 11, 2022. The methodological quality of the included research was evaluated using the Physiotherapy Evidence Database scale. This systematic review includes 16 studies. Eight studies received "excellent" ratings (≥6 points), 5 received "good" ratings (5 points), and 3 received "moderate" ratings (4 points). The findings showed that the most frequently examined performance factors in exercise training intervention strategies with handball players were balance (n = 5), agility (n = 8), speed (n = 9), and jumping performance (n = 10). Muscular strength (n = 4), shooting and dribbling skill (n = 3), and muscular endurance (n = 4) were the second most frequently investigated performance factors. Regarding speed and agility, exercise training considerably improved the physical fitness of handball players. However, evidence related to muscular strength, shooting ability, and dribbling skills was limited. To achieve the optimum standard, handball players must have strong physical and physiological qualities. Meanwhile, no evidence was found related to the impacts of exercise training on power, endurance, flexibility, body composition, cardiovascular fitness, and cardiorespiratory fitness. Regarding limitations, there is a need for more research with solid evidence to determine the impacts of exercise training interventions on athletes' performance in handball.
Fulltext Effects of neuromuscular training on athletes physical fitness in sports: A systematic review

Akbar S, Soh KG, Jazaily Mohd Nasiruddin N, Bashir M, Cao S, Soh KL

Front Physiol, 2022;13:939042.
PMID: 36213248 DOI: 10.3389/fphys.2022.939042

Objective: This review study aimed to assess the impact of neuromuscular training (NT) on athletes' physical fitness in sports. Methods: Three independent reviewers conducted a literature search in various databases: EBSCOHOST, PubMed, WOS, Mendeley, Scopus, ProQuest, Science Direct, additional references, and Google Scholar. The methodological quality was examined using Lubans' predetermined methods, and data that included trials were excluded. Results: This review included 18 well-conducted systematic studies from 144 relevant publications. These studies were reviewed and have been given a score of 6. Medium-risk studies were scored 3 or 4, while low-risk studies were scored 5 or 6. None of the studies had a high-risk bias. The NT intervention revealed that balance (n = 10) was the main characteristic of physical fitness that was evaluated, followed by agility (n = 6), muscular strength (n = 4), speed (n = 5), endurance and muscular power (n = 2). Subsequently, most studies used an intervention such as plyometric and strength training exercises to improve agility, balance, and muscular strength among athletes. Conclusion: This review implicated that (NT) focuses on exercises that enhance motor skills which aid athletes in moving their bodies according to their situational needs. The athletes' slower and faster directions influence their agility, muscular strength, and balance, essential for player performance. It is recommended for future research to investigate the effects of neuromuscular training (length of 12-weeks, frequency of 3 days per week and 90-min duration) on physical fitness components (coordination, reaction-time, flexibility, cardiovascular fitness, cardiorespiratory fitness and body composition) that are essential for all ages of male and female athletes in all sports.
Fulltext Effects of functional training on sprinting, jumping, and functional movement in athletes: A systematic review

Bashir M, Soh KG, Samsudin S, Akbar S, Luo S, Sunardi J

Front Physiol, 2022;13:1045870.
PMID: 36531163 DOI: 10.3389/fphys.2022.1045870

This systematic review aims to illuminate the effects of functional training on sprinting, jumping, and functional movements in athletes. A systematic search of electronic databases-that include PubMed, EBSCOhost (Sport Discus), SCOPUS, ProQuest, Google Scholar, and additional references-was carried out using keywords associated with functional training, jumping, sprinting, functional movement skills, and athletes, in compliance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) statement criteria. The Physiotherapy Evidence Database (PEDro) scale was used to measure the methodological quality of the studies included in the systematic review. Results: From a total of 220 studies, 15 included ones met all eligibility criteria and were scored between 4-5 points-considered as" moderate quality"-by the PEDro scale. Most studies recorded positive effects of functional training on athletes' sprinting, jumping, and functional movement. In contrast, a small number of studies did not find any positive effects of functional training on sprinting, squat jump, vertical jump, and countermovement jump due to the short duration and frequency of the training, as well as the lack of additional exercises that come with the interventions. Furthermore, the reviewed studies reveal that there is limited research within the literature on 5, 15, 25, and 50 m sprinting, squat jump, quadrant jump, and functional movement in athletes. Conclusion: Although the length of training interventions varied across studies in this systematic review, functional training interventions were found to help improve athletes' performance. The review reveals that training duration, intensity, and frequency are some critical variables that need to be taken into account when developing a successful functional training intervention for athletes. More studies are required to evaluate the influence of different accessible functional training durations on athletes' sprinting, jumping performance, and functional movement. Finally, further research needs to be done to investigate the impacts of functional training on performance and movement skills of male and female athletes at all levels in other sports. Systematic Review Registration: https://inplasy.com/inplasy-2022-5-0130/, identifier INPLASY202250130.
Mitigation of bio-corrosion characteristics of coronary artery stent by optimising fs-laser micromachining parameters

Chenrayan V, Palanisamy D, Mani K, Shahapurkar K, Elahi M Soudagar M, Fouad Y, et al.

Heliyon, 2024 Mar 30;10(6):e28057.
PMID: 38545133 DOI: 10.1016/j.heliyon.2024.e28057

Cardiovascular diseases, particularly coronary artery disease, pose big challenges to human life. Deployment of the stent is a preferable treatment for the above-mentioned disease. However, stents are usually made up of shape memory alloy called Nitinol. The poorer surface finish on the machined nitinol stents accelerates the migration of Nickel ions from the implanted nitinol stent, which is considered toxic and can lead to stenosis. The current study deals with controlling surface quality by minimising surface roughness and improving corrosion resistance. Femtosecond laser (fs-laser 10-15 s) micromachining was employed to machine the Nitinol surface to achieve sub-micron surface roughness. The Grey relational analysis (GRA)-coupled design of the experimental technique was implemented to determine optimal levels of four micromachining parameters (laser power, pulse frequency, scanning speed, and scanning pattern) varied at three levels to achieve minimum surface roughness and to maximise the volume ablation. The results show that to yield minimum surface roughness and maximum volume ablation, laser power and scanning speed are in a higher range. In contrast, the pulse frequency is lower, and the scanning pattern is in a zig-zag manner. ANOVA results manifest that scanning speed is the predominant factor in minimising surface roughness, followed by pulse frequency. Furthermore, the corrosion behaviour of the machined nitinol specimens was evaluated, and the results show that specimens with lower surface roughness had lower corrosion rates.
Fulltext Effect of Hydrophilic Polymers on Complexation Efficiency of Cyclodextrins in Enhancing Solubility and Release of Diflunisal

Bashir M, Syed HK, Asghar S, Irfan M, Almalki WH, Menshawi SA, et al.

Polymers (Basel), 2020 Jul 15;12(7).
PMID: 32679660 DOI: 10.3390/polym12071564

The effects of three hydrophilic polymers, namely, carboxymethyl cellulose sodium (CMC-Na), polyvinyl alcohol (PVA) and poloxamer-188 (PXM-188) on the solubility and dissolution of diflunisal (DIF) in complexation with β-cyclodextrin (βCD) or hydroxypropyl β-cyclodextrin (HPβCD), were investigated. The kneading method was used at different drug to cyclodextrin weight ratios. Increases in solubility and drug release were observed with the DIF/βCD and DIF/HPβCD complexes. The addition of hydrophilic polymers at 2.5, 5.0 and 10.0% w/w markedly improved the complexation and solubilizing efficiency of βCD and HPβCD. Fourier-transform infrared (FTIR) showed that DIF was successfully included into the cyclodextrin cavity. Differential scanning calorimetry (DSC) and X-ray diffractometry (XRD) confirmed stronger drug amorphization and entrapment in the molecular cage of cyclodextrins. The addition of PVA, CMC-Na or PXM-188 reduced further the intensity of the DIF endothermic peak. Most of the sharp and intense peaks of DIF disappeared with the addition of hydrophilic polymers. In conclusion, PXM-188 at a weight ratio of 10.0% w/w was the best candidate in enhancing the solubility, stability and release of DIF.

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