Studies of genetic mutations that have been used in predicting glioma prognosis have revealed a complex relationship between clinical and genetic factors. Epidermal growth factor (EGF) and the NAT2 gene play a central role in carcinogenesis. An adenine (A) to guanine (G) single nucleotide polymorphism at position 61 in the 5'-untranslated region (5'-UTR) of the EGF gene has been found to be associated with levels of EGF production, and the mutations in the NAT2 gene have been postulated as a risk factor for cancer. We investigated EGF and the NAT2 gene in 13 glioma tissue samples and 12 normal controls. In the EGF 5'-UTR 61G polymorphism, the heterozygote GA was the most common genotype in the glioma patients. In the NAT2 polymorphism at nucleotide position 857G/A, the G allele and the GG genotype were the most prevalent forms in both the glioma and normal samples. We did not find any homozygous AA genotypes in the glioma patients. Based on this preliminary evidence, the EGF 5'-UTR at position 61 and the NAT2 SNP at position 857 polymorphisms are associated with increased risk for glioma.
Bilateral presentation of multiple evanescent white dot syndrome (MEWDS) is a rare occurrence. We report a case of bilateral multiple evanescent white dot syndrome in a young female patient with asymmetrical manifestation. She presented with sudden onset of right eye central blurring of vision and dyschromatopsia. Fundus examination however showed bilateral multiple grey-white intra-retinal punctate lesions with an asymmetrical manifestation of the swollen optic disc and foveal granularity over the right. Spectral Domain Optical Coherence Tomography (SD-OCT) showed the presence of juxta foveal subretinal fluid and disrupted inner segment-outer segment (IS-OS) junction over the right eye. The patient had a spontaneous complete recovery within six weeks' time.
This study showed that bio-functional silver nanoparticles (AgNPs) and zinc oxide nanoparticles (ZnONPs) were synthesized in aqueous extracts of Gymnema sylvestre leaves and tested for toxicity assessment against triple-negative breast cancer cells (TNBC). Biofunctional nanoparticle (NPs) samples were characterized using UV-Vis spectroscopy, FT-IR, XRD, SEM, and TEM. The results showed that the phytofabrication of AgNPs resulted in a dark brown, UV-vis maximum absorbance peak at 413 nm. The AgNPs were crystalline and spherical, with sizes ranging from 20 to 60 nm, as confirmed by the XRD pattern and TEM images. Another phytofabrication of ZnONPs exhibited a white precipitate corresponding to a UV-Vis maximum absorption peak at 377 nm and a fine micro flower morphology with a particle-sized tribution between 100 and 200 nm. In addition, FT-IR spectra showed that bioorganic compounds are associated with NPs that respond to reduced Ag+ ions and AgNPs tabilizers. Invitro cytotoxicity studies revealed the potent anti-cancer effects of phytofabricated AgNPs and ZnONPs on TNBC cells. Furthermore, the AO/EB double staining assay results proved that apoptotic cells are distinguished by greenish-yellow fluorescence of the cell nuclei with IC50 concentrations of 44 ± 0.8 µg/mL for AgNPs and 26.2 ± 0.5 µg/mL for ZnONPs, respectively. Based on our results, we expect that the anticancer function of the biofunctional NPs is due to the apoptotic activation of TNBC cells by increased ROS. Therefore, the presented study demonstrated that biofunctional AgNPs and ZnONPs have excellent prospects for the anti-cancer activity that can be used in pharmaceutical and medical fields.
In neurosurgery and ear, nose and throat surgery the application of computerised navigation systems for guiding operations has been expanding rapidly. However, suitable models to train surgeons in using navigation systems are not yet available. We have developed a technique using an industrial, rapid prototyping process from which accurate spatial models of the cranium, its contents and pathology can be reproduced for teaching. We were able to register, validate and navigate using these models with common available navigation systems such as the Medtronic StealthStation S7®.
This study aims to obtain the relationship between density and compressive strength of foamed concrete. Foamed concrete is a preferred building material due to the low density of its concrete. In foamed concrete, the compressive strength reduces with decreasing density. Generally, a denser foamed concrete produces higher compressive strength and lower volume of voids. In the present study, the tests were carried out in stages in order to investigate the effect of sand-cement ratio, water to cement ratio, foam dosage, and dilution ratio on workability, density, and compressive strength of the control foamed concrete specimen. Next, the test obtained the optimum content of processed spent bleaching earth (PSBE) as partial cement replacement in the foamed concrete. Based on the experimental results, the use of 1:1.5 cement to sand ratio for the mortar mix specified the best performance for density, workability, and 28-day compressive strength. Increasing the sand to cement ratio increased the density and compressive strength of the mortar specimen. In addition, in the production of control foamed concrete, increasing the foam dosage reduced the density and compressive strength of the control specimen. Similarly with the dilution ratio, the compressive strength of the control foamed concrete decreased with an increasing dilution ratio. The employment of PSBE significantly influenced the density and compressive strength of the foamed concrete. An increase in the percentage of PSBE reduced the density of the foamed concrete. The compressive strength of the foamed concrete that incorporated PSBE increased with increasing PSBE content up to 30% PSBE. In conclusion, the compressive strength of foamed concrete depends on its density. It was revealed that the use of 30% PSBE as a replacement for cement meets the desired density of 1600 kg/m3, with stability and consistency in workability, and it increases the compressive strength dramatically from 10 to 23 MPa as compared to the control specimen. Thus, it demonstrated that the positive effect of incorporation of PSBE in foamed concrete is linked to the pozzolanic effect whereby more calcium silicate hydrate (CSH) produces denser foamed concrete, which leads to higher strength, and it is less pore connected. In addition, the regression analysis shows strong correlation between density and compressive strength of the foamed concrete due to the R2 being closer to one. Thus, production of foamed concrete incorporating 30% PSBE might have potential for sustainable building materials.