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Fulltext Knockdown of Stromal Interaction Molecule 1 (STIM1) Suppresses Acute Myeloblastic Leukemia-M5 Cell Line Survival Through Inhibition of Reactive Oxygen Species Activities

Algariri ES, Mydin RBSMN, Moses EJ, Okekpa SI, Rahim NAA, Yusoff NM

Turk J Haematol, 2023 Feb 28;40(1):11-17.
PMID: 36404683 DOI: 10.4274/tjh.galenos.2022.2022.0246

OBJECTIVE: This study aimed to investigate the role of the stromal interaction molecule 1 (STIM1) gene in the survival of the acute myeloblastic leukemia (AML)-M5 cell line (THP-1).
MATERIALS AND METHODS: The STIM1 effect was assessed via dicersubstrate siRNA-mediated STIM1 knockdown. The effect of STIM1 knockdown on the expression of AKT and MAPK pathway-related genes and reactive oxygen species (ROS) generation-related genes was tested using real-time polymerase chain reaction. Cellular functions, including ROS generation, cell proliferation, and colony formation, were also evaluated following STIM1 knockdown.
RESULTS: The findings revealed that STIM1 knockdown reduced intracellular ROS levels via downregulation of NOX2 and PKC. These findings were associated with the downregulation of AKT, KRAS, MAPK, and CMYC. BCL2 was also downregulated, while BAX was upregulated following STIM1 knockdown. Furthermore, STIM1 knockdown reduced THP-1 cell proliferation and colony formation.
CONCLUSION: This study has demonstrated the role of STIM1 in promoting AML cell proliferation and survival through enhanced ROS generation and regulation of AKT/MAPK-related pathways. These findings may help establish STIM1 as a potential therapeutic target for AML treatment.
Fulltext Assessing wellbeing: Profiling and socioeconomic status of Kenyir Lake side community, Malaysia

Ab Manaf N, Sa'at NH, A Rahim NAA, Kamaruddin SNAA, Abdullah SS, Omar K

Heliyon, 2023 Jun;9(6):e16399.
PMID: 37303545 DOI: 10.1016/j.heliyon.2023.e16399

The Kenyir Lake side community offers numerous advantages to the local community. However, the problems of backwardness and poverty have been identified as the government's main challenges in its efforts to develop the community and maximise the advantages. Therefore, this study was conducted to identify the Kenyir Lake community's profile and assess its well-being. The study was conducted in three sub-districts near Tasik Kenyir, namely Kuala Berang, Hulu Telemong, and Jenagor, with a total of 510 respondents, who are heads of households (HOH). This study was carried out using a quantitative approach using questionnaire with a simple random sampling method. The findings of this study offered demographic profiling and discovered nine indicators of well-being, namely 1) Life Achievement, 2) Health Level, 3) Family Relationship, 4) Community Relationship, 5) Spiritual Level, 6) Safety Level and Social Problems, 7) Income or Finance, 8) Basic Facilities, and 9) Communication Technology. The study found that most respondents are satisfied with their lives now compared to 10 years ago. This study will help many parties to develop the Kenyir Lake Side Community, starting from the local authorities to the highest administration in the country.
Fulltext Knowledge and Perceptions of Blood Safety among Blood Donors in Kelantan, Malaysia

Tan PP, Fauzi HM, Bahar R, Chang CT, Rahim NAA

Malays J Med Sci, 2019 Nov;26(6):127-136.
PMID: 31908594 MyJurnal DOI: 10.21315/mjms2019.26.6.13

Background: Unsafe blood products may cause transfusion-transmissible infections. This study aimed to evaluate the knowledge and perceptions of blood donors regarding blood safety.
Methods: This was a cross-sectional study conducted in the Kelantan state of Malaysia. The questionnaire comprised 39 questions that covered areas such as donors' social demographic information, knowledge of transfusion-transmitted diseases, blood screening and donor eligibility and perceptions towards blood safety. The knowledge score was categorised as good or poor.
Results: Of the 450 distributed questionnaires, 389 were suitable for analysis. Only 18.5% of the donors had good knowledge, with 81.5% having poor knowledge. Less than 30% were aware that people with multiple sexual partners, bisexual people and male homosexual people are permanently deferred from blood donation. Only 29.4% agreed that donors are responsible if their blood causes infection. Furthermore, 39.3% assumed that they could check their HIV status through blood donation, and 10.3% and 5.4% of the respondents believed that donors are free from infection if they wear a condom during sex or only have oral sex when involved in prostitution, respectively.
Conclusion: Poor knowledge and notable misperceptions concerning safe blood donation were found among blood donors. The Ministry of Health should incorporate safe blood education in future public awareness programmes.
Fulltext Unveiling the Diversity of Periphytic Cyanobacteria (Cyanophyceae) from Tropical Mangroves in Penang, Malaysia

Rahim NAA, Mohd Sidik Merican FM, Radzi R, Omar WMW, Nor SAM, Broady P, et al.

Trop Life Sci Res, 2023 Sep;34(3):57-94.
PMID: 37860087 DOI: 10.21315/tlsr2023.34.3.4

Cyanobacteria are one of the most important groups of photoautotrophic organisms, contributing to carbon and nitrogen fixation in mangroves worldwide. They also play an important role in soil retention and stabilisation and contribute to high plant productivity through their secretion of plant growth-promoting substances. However, their diversity and distribution in Malaysian mangrove ecosystems have yet to be studied in detail, despite Malaysia hosting a significant element of remaining mangroves globally. In a floristic survey conducted in Penang, peninsular Malaysia, 33 morphospecies of periphytic cyanobacteria were identified and described for the first time from a mangrove ecosystem in Malaysia. Sixteen genera, comprising Aphanocapsa, Chroococcus, Chroococcidiopsis, Cyanobacterium, Desmonostoc, Geitlerinema, Leptolyngbya, Lyngbya, Microcystis, Myxosarcina, Oscillatoria, Phormidium, Pseudanabaena, Spirulina, Trichocoleus and Xenococcus, were obtained from field material growing on diverse natural and artificial substrata. Oscillatoriales was the dominant order with Phormidium the dominant genus at nine of the 15 sampling sites examined. Three of the morphospecies, Aphanocapsa cf. concharum, Xenococcus cf. pallidus and Oscillatoria pseudocurviceps, are rare and poorly known morphospecies worldwide. Chroococcus minutus, Phormidium uncinatum, P. amphigranulata, and some species of Oscillatoriales are considered as pollution indicator species. This study provides important baseline information for further investigation of the cyanobacterial microflora present in other mangrove areas around Malaysia. A complete checklist will enhance understanding of their ecological role and the potential for benefits arising from useful secondary metabolites or threats via toxin production to the ecosystem.
Fulltext Data on ectoparasites infestation on small mammals from different habitats in east-coast Peninsular Malaysia

Ahmad NII, Rahim NAA, Roslan A, Adrus M, Ahamad M, Hassan M, et al.

Data Brief, 2020 Jun;30:105621.
PMID: 32395585 DOI: 10.1016/j.dib.2020.105621

This data article presents on the ectoparasites infestation on small mammals in Peninsular Malaysia. The dataset on ectoparasites infestation is important because it raises a major medical concern regarding the spread of potentially zoonotic disease from wildlife to human. Tick and chigger are the primary ectoparasites as reservoirs of vector-borne diseases found on small mammals in Malaysia. These small mammals that are infested with ectoparasites occupy various types of habitats, including human settlements, could be of community health risks as the carriers of potentially zoonotic diseases. Field samplings were conducted from February 2015 to February 2016 in three different ecological habitats of mixed dipterocarp forest, coastal forest and insular forest, in Terengganu, Malaysia. A total of 35 and 22 species of bats and rodents respectively were captured and examined for ectoparasites. Twenty-three species of bats and 16 species of small mammal were recorded as hosts for at least one species of ectoparasites. These findings show that the highest ectoparasite infestation occurred on bat community.
Recombinant activated factor VII (rFVIIa) in refractory haemorrhage for non-haemophiliacs: an eleven-year single-centre experience

Shah NM, Chong SE, Yusoff SM, Mazlan MZ, Johan KB, Azman N, et al.

BMC Hematol, 2018;18:34.
PMID: 30498571 DOI: 10.1186/s12878-018-0126-z

Background: Massive bleeding is one of the commonest salvageable causes of death. The search for an ideal haemostatic agent during massive bleeding is still ongoing. One of the novel haemostatic medications is recombinant activated factor VII (rFVIIa). To date, the usage of rFVIIa during massive haemorrhage among non-haemophiliac patients remains off-label. The aim of this study is to report our experience in using rFVIIa to treat refractory bleeding.
Methods: Medical records of all patients treated with rFVIIa for massive bleeding over an eleven-year period in a single institution were recorded. Treatment indications, 24-h and 30-day mortality, changes in transfusion needs and coagulation profiles after rFVIIa administration were analysed.
Results: rFVIIa were administered in 76 patients. Of these, 41 (53.9%) were non-surgical bleeding, followed by 22 patients (28.9%) with trauma, other surgery bleedings in 9 patients (11.8%) and 4 patients (5.4%) with peripartum haemorrhage. Total survival rate was 78.9% within 24 h and 44.7% over 30 days. Among all these patients who had received rFVIIa due to life-threatening haemorrhage, blood and blood product requirements were significantly reduced (P