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Could choline chloride-citric acid monohydrate molar ratio in deep eutectic solvent affect structural, functional and antioxidant properties of pectin?

Shafie MH, Gan CY

Int J Biol Macromol, 2020 Apr 15;149:835-843.
PMID: 32027904 DOI: 10.1016/j.ijbiomac.2020.02.013

The deep eutectic solvents (DESs), which were made from different molar ratios (3:1, 2:1, 1:1, 1:2, 1:3) of choline chloride and citric acid monohydrate, were used as media for the pectic polysaccharide extraction from Averrhoa bilmbi (ABP). The physico-chemical, structural, functional and antioxidant properties of ABP were subsequently determined. The ABP was found to be xylogalacturonan. Moreover, results showed that different structures (i.e. linearity of pectin and branch size) of ABP were obtained, hence, affecting the solubility and functional properties due to the surface availability and steric effect. In addition, when increasing the molar ratio of citric acid monohydrate in DES, lower pH and higher TPC values were observed. These values were correlated with antioxidant activities (i.e. free radical scavenging activity and ferric reducing antioxidant power) of ABP. In conclusion, the molar ratio of the DES components plays an important role in extracting ABP with the aforementioned properties.
Microwave-assisted extraction of polysaccharides from Micromelum minutum leaves using citric acid monohydrate-glycerol based deep eutectic solvents and evaluation of biological activities

Md Yusoff MH, Shafie MH

Anal Chim Acta, 2024 Dec 01;1331:343351.
PMID: 39532430 DOI: 10.1016/j.aca.2024.343351

BACKGROUND: The extraction of polysaccharides using an acidic extraction media has been extensively reported, highlighting its effectiveness in yielding high-quality polysaccharides. A higher concentration of acidic solution could hydrolyze the structure of polysaccharide, while a low concentration reduces the extraction efficiency. Despite this challenges, deep eutectic solvents (DES) were introduced as an alternative extraction medium due to additional interactions such as inter and intra-molecular interactions, Van de Waals, hydrogen bond, and electrostatic interactions, which could improve the polysaccharide extraction efficiency and biological activities. Furthermore, the extraction conditions such as extraction medium and extraction parameters could affect the properties of polysaccharides as well as influence their structure-activity relationship for biological activities.
RESULTS: The result showed that the microwave-assisted extraction of Micromelum minutum leaf polysaccharide (MMLP) using DES as an extraction media (MMLP-DES) gave a higher yield (improvement of 101.20 %) than citric acid monohydrate (CAM) (MMLP-CAM) and required a lower percentage of microwave power (19.83 % less) and time (0.78 min less). The properties of MMLPs significantly differ based on their pH, molecular weight, viscosity, degree of esterification and monosaccharide molar ratio which influenced the biological activities. Compared to MMLP-CAM, MMLP-DES had a more branched and less linear structure. The bioactivities study revealed that MMLP-DES exhibited higher antioxidant and anti-α-amylase activities (i.e.
, DPPH: 74.52 %, FRAP: 2.87 mM FeSO4 and α-amylase inhibition: 86.23 %) compared to MMLP-CAM (i.e.
, DPPH: 49.33 %, FRAP: 1.49 mM FeSO4, and α-amylase inhibition: 81.76 %). The mechanism and structure-activity relationship of MMLPs on bioactivities were also hypothesized.
SIGNIFICANCE: Based on our previous study, the citric acid monohydrate-glycerol based DES as an extraction medium has enhanced the extraction yield of polysaccharides from M. minutum. This study highlights the DES combined with microwave-assisted extraction to improve the yield of MMLP and evaluate the biological activities compared to CAM as a classical solvent. In conclusion, the DES showed the advantages for extraction of polysaccharides with desired biological activities.
Star anise (Illicium verum Hook. F.) polysaccharides: Potential therapeutic management for obesity, hypertension, and diabetes

Alias AHD, Shafie MH

Food Chem, 2024 Dec 01;460(Pt 1):140533.
PMID: 39053285 DOI: 10.1016/j.foodchem.2024.140533

This study explores the extraction of polysaccharides from star anise (Illicium verum Hook. f.) with its anti-obesity, antihypertensive, antidiabetic, and antioxidant properties. The aim is to optimize the extraction conditions of star anise polysaccharides (SAP) utilizing propane alcohols-based deep eutectic solvents and microwave-assisted methods. The optimized conditions resulted in an extraction yield of 5.14%. The characteristics of acidic pectin-like SAP, including high viscosity (44.86 mPa s), high oil-holding capacity (14.39%), a high degree of esterification (72.53%), gel-like properties, highly amorphous, a high galacturonic acid concentration, and a highly branching size polysaccharide structure, significantly contribute to their potent inhibition of pancreatic lipase (86.67%), angiotensin-converting enzyme (73.47%), and α-glucosidase (82.33%) activities as well as to their antioxidant properties of azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS, 34.94%) and ferric ion reducing antioxidant power (FRAP, 0.56 mM FeSO4). Therefore, SAP could be used as a potential therapeutic agent for obesity, hypertension, and diabetes mellitus management.
Deep eutectic solvents (DES) mediated extraction of pectin from Averrhoa bilimbi: Optimization and characterization studies

Shafie MH, Yusof R, Gan CY

Carbohydr Polym, 2019 Jul 15;216:303-311.
PMID: 31047070 DOI: 10.1016/j.carbpol.2019.04.007

The Box-Behnken design was applied to optimize the extraction of pectin from Averrhoa bilimbi (ABP) using deep eutectic solvents (DES). The four variables of extraction were percentage of DES (X1), extraction time (X2), temperature (X3), and molar ratio of DES components (X4). The quadratic regression equation was established as a predicted model with R2 value of 0.9375. The optimal condition was X1 = 3.74% (w/v), X2 = 2.5 h, X3 = 80 °C, and X4 = 1:1. No significant difference between the predicted (14.70%) and experimental (14.44%) maximum yield of sample was noted. Characterization of physico-chemical properties characterization of ABP was performed. The main components of ABP were galacturonic acids, arabinoses, and xyloses. ABP also showed good functional properties such as water holding capacity (3.70 g/g), oil holding capacity (2.40 g/g), and foaming capacity (133.33%). The results also showed that ABP exhibited free radical scavenging activity (41.46%) and ferric reducing antioxidant power (1.15 mM).
Averrhoa bilimbi pectin-based edible films: Effects of the linearity and branching of the pectin on the physicochemical, mechanical, and barrier properties of the films

Shafie MH, Yusof R, Samsudin D, Gan CY

Int J Biol Macromol, 2020 Nov 15;163:1276-1282.
PMID: 32673725 DOI: 10.1016/j.ijbiomac.2020.07.109

The potential of Averrhoa bilimbi pectin (ABP) as a source of biopolymer for edible film (EF) production was explored, and deep eutectic solvent (DES) (1% w/w) containing choline chloride-citric acid monohydrate at a molar ratio of 1:1 was used as the plasticizer. The EF-ABP3:1, which was produced from ABP with large branch size, showed a higher value of melting temperature (175.30 °C), tensile stress (7.32 MPa) and modulus (33.64 MPa). The EF-ABP3:1 also showed better barrier properties by obtaining the lowest water vapor transmission rates (1.10-1.18 mg/m2.s) and moisture absorption values (2.61-32.13%) depending on the relative humidity compared to other EF-ABPs (1.39-1.83 mg/m2.s and 3.48-51.50%, respectively) that have linear structure with smaller branch size. From these results, it was suggested that the galacturonic acid content, molecular weight, degree of esterification and pectin structure of ABP significantly influenced the properties of EFs. The interaction of highly branched pectin chains was stronger than the linear chains, thus reduced the effect of plasticizer and produced a mechanically stronger EF with better barrier properties. Hence, it was suggested that these EFs could be used as alternative degradable packaging/coating materials.
Characterization of bio-based plastic made from a mixture of Momordica charantia bioactive polysaccharide and choline chloride/glycerol based deep eutectic solvent

Shafie MH, Samsudin D, Yusof R, Gan CY

Int J Biol Macromol, 2018 Oct 15;118(Pt A):1183-1192.
PMID: 29944943 DOI: 10.1016/j.ijbiomac.2018.06.103

Momordica charantia bioactive polysaccharide (MCBP) was used as an alternative source for the production of bio-based plastics (BPs) with choline chloride/glycerol-based deep eutectic solvent (DES) as a plasticizer. In this study, MCBP was initially extracted using 0.1 M citric acid at temperature 80 °C for 2 h, precipitated using ethanol, and then lyophilized. Subsequently, seven BPs were prepared: MCBP without plasticizer (MCBP), with 1% (w/w) of glycerol (MCBP-G), or with 1% (w/w) of DES at different choline chloride/glycerol molar ratios (i.e. 1.5:1, 1:1, 1:1.5, 1:2, and 1:3). The properties of these BPs were then investigated. Results showed that the tensile strains, stresses and moduli were in the range of 1.3-13.3%, 4.8-19.1 MPa and 132-2487 MPa, respectively. The melting temperatures were found in the range of 92.6-111.4 °C whereas the moisture absorptions and water vapour transmission rates (WVTR) of BPs were 1.4-6.5% and 3.6-5.4 mg/m2·s, respectively. The results also showed that these BPs exhibited bioactivities, such as microbial inhibitory activity (19.5-32.3 mm), free radical scavenging activity (10.3-18.3%) and ferric reducing antioxidant power (FRAP, 16.1-20.0 mM). In addition, it was observed that using DES as a plasticizer had improved the properties of BP, such as tensile strain (354.7-937.5%), melting temperature (4.6-20.3%), radical scavenging activity (0.6-88.6%), FRAP (0.9-18.7%) and antimicrobial activity (12.3-33.6%) compared to MCBP, due to the fact DES has caused different degrees of plasticization via hydrogen bonds and ionic bonds with the polymer chains, and induced a lower pH condition. Therefore, it was suggested that these BPs with DES could contribute to food preservation properties.
Screening and confirmation tests for SARS-CoV-2: benefits and drawbacks

Shafie MH, Antony Dass M, Ahmad Shaberi HS, Zafarina Z

Beni Suef Univ J Basic Appl Sci, 2023;12(1):6.
PMID: 36647397 DOI: 10.1186/s43088-023-00342-3

BACKGROUND: Coronavirus disease 2019 is a pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection that emerged in late 2019 and has activated an ongoing international public health emergency. SARS-CoV-2 was discovered in Wuhan, China, in December 2019 and rapidly spread to other cities and countries. Currently, SARS-CoV-2 diagnostic tests have relied heavily on detecting viral genes, antigens, and human antibodies. Hence, this review discusses and analyses the existing screening and confirmation tests for SARS-CoV-2, including the real-time reverse transcriptase polymerase chain reaction (RT-PCR), lateral flow immunoassay (LFIA), and enzyme-linked immunosorbent assay (ELISA).
MAIN BODY: The illustrations of each testing were presented to provide the readers with an understanding of the scientific principles behind the testing methods. The comparison was made by highlighting the advantages and disadvantages of each testing. ELISA is ideal for performing the maximum population screening to determine immunological capacity, although its inability to provide reliable results on the status of the infection. Recently, LFIA has been approved as a quicker way of determining whether a patient is infected at the analysis time without using particular instruments and non-laboratory settings. RT-PCR is the gold-standard approach in terms of sensitivity and specificity.
CONCLUSION: However, the combination of LFIA or ELISA with RT-PCR is also proposed in this review to obtain an adequate level of sensitivity and specificity.
Fulltext Knowledge and beliefs about breastfeeding are not determinants for successful breastfeeding

Ishak S, Adzan NA, Quan LK, Shafie MH, Rani NA, Ramli KG

Breastfeed Med, 2014;9(6):308-12.
PMID: 24893127 DOI: 10.1089/bfm.2013.0124

A cross-sectional prospective study was performed to assess knowledge and attitude toward breastfeeding among mothers in a tertiary hospital in Malaysia and its influence on their breastfeeding practices. Two hundred thirteen women who had delivered healthy babies at term were enrolled. A structured questionnaire containing demographic data and the Iowa Infant Feeding Attitude Score were used, followed by a telephone interview after 8 weeks to determine the feeding outcome. Women of Malay ethnicity with higher education level who had received breastfeeding counseling had a significantly more favorable attitude toward breastfeeding. Ethnicity was found to be a significant determinant in the success of breastfeeding, whereas returning to work was a major reason for discontinuing breastfeeding. In ensuring a successful breastfeeding practice, apart from knowledge and attitude, issues surrounding culture and traditions as well as improving deliverance of readily available support should be addressed.
Microwave-assisted extraction of polysaccharide from Cinnamomum cassia with anti-hyperpigmentation properties: Optimization and characterization studies

Al-Ajalein AAS, Shafie MH, Yap PG, Kassim MA, Naharudin I, Wong TW, et al.

Int J Biol Macromol, 2023 Jan 31;226:321-335.
PMID: 36502951 DOI: 10.1016/j.ijbiomac.2022.12.023

The anti-hyperpigmentation effect and tyrosinase inhibitory mechanism of cinnamon polysaccharides have not been reported. The current study focused on the extraction of polysaccharides from Cinnamomum cassia bark using microwave-assisted approach and optimization of the extraction process (i.e., microwave power, irradiation time and buffer-to-sample ratio) by Box-Behnken design to obtain a high yield of polysaccharides with high sun protection factor (SPF), anti-hyperpigmentation and antioxidant activities. The extracted pectic-polysaccharides had low molecular weight and degree of esterification. The optimal extraction process had polysaccharides characterized by (a) monophenolase inhibitory activity = 97.5 %; (b) diphenolase inhibitory activity = 99.4 %; (c) ferric reducing antioxidant power = 4.4 mM; (d) SPF = 6.1; (e) yield = 13.7 %. The SPF, tyrosinase inhibitory and antioxidant activities were primarily contributed by the polysaccharides. In conclusion, the polysaccharides from C. cassia could be an alternative therapeutic source for skin hyperpigmentation treatment.