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Fulltext Hypertension genes among orang asli in peninsular Malaysia and ethno-medicinal plant

Sholehah, A. R., Ramle, A., Mohd Tajuddin, A., Wan Rohani, W. T., Jamilah, M. S., Razifah, M.

Asian Journal of Medicine and Biomedicine, 2018;2(1):0-0.
MyJurnal

The prevalence and incidence rate of hypertension among Orang Asli had been increasing due to
modernization and assimilation of outside practices such as intake of high cholesterol food, alcohol, and
smoking. Orang Asli utilize their herbs or plants to treat some illness due to the factor of logistics and the
knowledge on these nature resources since decades ago that is carried out from their ancestors. In this review
paper, hypertension genes of Orang Asli in Peninsular Malaysia and the utilization of ethno-medicinal plants
in reducing the clinical manifestation in hypertension were deliberated. There are quite a number of related
hypertensive genes particularly in renin-angiotensin-aldosterone system (RAAS) playing a pivotal role in
pathogenesis of hypertension. A genome-wide association studies showed potential candidate genes in
hypertension among Orang Asli in Peninsular Malaysia. However there is yet molecular study on these genes
among Orang Asli with their unique genetic profile. Noteworthy information on mechanism of ethnomedicine in treating hypertension are scarce, even the efficacy of modern medicine in treating hypertension
on Orang Asli are rare. Therefore, study on efficacy of ethno-medicine plant by Orang Asli and the
regulation effect on hypertension genes are needed to be further explored and elucidated.
Fulltext Study of association ofTGF-ß1 polymorphism with breast density in a tertiary medical center of Malaysia

Wan Rohani, W. T., Amiratul Athirah S., Nasriah A., Mohaslida, M., Tengku Fatimah Murniwati T. M., Norhasiza M. J., et al.

Medicine & Health, 2017;12(2):312-320.
MyJurnal

Pengesanan awal risiko kanser payudara menggunakan mammografi dapat
mengurangkan kos pengurusan rawatan dan prognosis penyakit ini. Paras
ketumpatan payudara yang dikesan menggunakan mammogram memberi
potensi faktor penyebab kepada risiko kanser payudara. Penghasilan sel epitelial
pada mamari dapat ditentukan oleh faktor genetik. Gen transforming growth
factor-beta (TGF-ß) telah dikenalpasti terlibat di dalam regulasi proliferasi sel dan
pembahagian sel. Kajian ini bertujuan untuk mengukur hubung kait polimorfisme
TGF-ß1 dengan ketumpatan payudara di kalangan wanita yang menjalani saringan
mamografi. Pengesanan genotip bagi tiga polimorfisme TGF-ß1 yang dikenali
sebagai rs1800469, rs1800470 dan rs4803455 dilakukan dengan teknik PCR-RFLP.
Frekuensi alel dan genotip dikira bagi kumpulan kawalan yang terdiri dari kalangan
wanita yang telah dikelaskan BIRADS 1 dan BIRADS 2, manakala kumpulan kes
terdiri dari kalangan wanita yang telah dikelaskan BIRADS 3 dan BIRADS 4.Dua
polimorfisme (rs1800469 dan rs1800470) telah menunjukkan hubung kait yang
signifikan dengan ketumpatan payudara dengan nilai P= 0.004 dan 0.003. Namun
begitu polimorfisme yang ketiga iaitu rs4803455 tidak menunjukkan hubung kait
(nilai P=0.090). Analisa hubung kait haplotaip mencadangkan haplotaip GAA
berupaya memberi kecenderungan ketumpatan payudara (nilai P=0.02, OR=2.21
[1.07-4.55]) berbanding haplotaip AAC yang berupaya untuk memberi kesan
perlindungan dari penghasilan ketumpatan payudara (nilai P=0.004, OR=0.40 [0.21-0.77]). Data asas hubung kait dan haplotaip ini memberi penjelasan kaitan
polimorfisme TGF-ß1 dengan ketumpatan payudara dan memberi maklumat fungsi
polimorfisme di dalam mempengaruhi pembentukan ketumpatan payudara.
Haplotype analysis of leptin gene polymorphisms in obesity among Malays in Terengganu, Malaysia population

Wan Rohani WT, Aryati A, Amiratul Athirah S

Med J Malaysia, 2018 10;73(5):281-285.
PMID: 30350805 MyJurnal

INTRODUCTION: The prevalence of overweight and obesity has developed the critical global threat which leads to metabolic risks and mortality. A Leptin hormone that regulates the food intake as well as food expenditure is encoded by Leptin gene. The gene has shown a pivotal role in obesity pathogenesis. This study was sought to determine the SNPs and haplotype association of the Leptin gene that were assigned as G2548A, H1328080, and A19G with obesity among Malays in Terengganu, Malaysia.
METHODOLOGY: This study comprised of 249 participants (148 overweight/ obese as a case group and 101 lean participants as controls). The PCR-RFLP technique was performed to distinguish the genotype distribution of Leptin gene polymorphisms. The allele and genotype frequencies were assessed for single and haplotype analyses.
RESULT: Single association analysis of G2548A (P=0.74), A19G (P=0.38), and H1328080 (P=0.56) polymorphisms yielded no statistically significant association. However, haplotype association analysis showed a suggestive indication of AAG haplotype (G2548A, H1328080, and A19G sequence) with susceptibility effect towards obesity predisposition [P=0.002, OR=8.897 (1.59-9.78)].
CONCLUSION: This data on single and haplotype might disclose the preliminary exposure and pave the way for the obesity development with an evidence of revealed susceptibility to obesity.
Association of solute carrier family 2, member 9 (SLC2A9) genetic variant rs3733591 with gout in a Malay sample set

Wan Rohani WT, Mahfudzah A, Nazihah MY, Tan HL, Wan Syamimee WG, Amanda Jane PG, et al.

Med J Malaysia, 2018 10;73(5):307-310.
PMID: 30350810 MyJurnal

INTRODUCTION: Gout is one of the most common inflammatory arthritis in Malaysia. It is due to persistent hyperuricemia that leads to the formation and deposition of intra- and periarticular monosodium urate crystals either due to excessive production or insufficient excretion of uric acid. Incidence and prevalence of gout is increasing worldwide, with a higher rate among men compared to women. Malay is the largest ethnic group in Malaysia, followed by Chinese and Indian. SLC2A9 is a renal urate transporter that controls renal uric acid excretion and genetic variants in SLC2A9 are associated with the risk of gout in several populations. This study aimed to test if the SLC2A9 variant (R265H, rs3733591) is also associated with gout among Malays in Malaysia.
METHODOLOGY: A total of 89 patients with gouty arthritis and 100 normal subjects who consented and were recruited in this study. The serum urate and creatinine were measured. The SNP genotyping was performed using PCR-RFLP method for rs3733591 and BST 1236 was used as a restriction enzyme to cut the targeted amplicons.
RESULT: SLC2A9 variant was associated with gout, p-value of 0.007, OR=4.713 [95%CI 1.530-14.513], however this association was not significant after adjustment for age and gender with p=0.465 (OR=1.950; 95%CI[0.325-11.718]).
CONCLUSION: Our data suggest that the genetic variant of SLC2A9 may contribute to the susceptibility of gout among Malays in Malaysia.