Five new limonoids, swieteliacates A-E (1-5) and a tirucallane-type triterpenoid, swietesenin (6), together with four known compounds (7-10) were isolated from fruit of Swietenia macrophylla. Their structures were determined by spectroscopic analyses. The new compounds were tested in vitro for their cytotoxic effects against five human cancer cell lines. Compound 2 exhibited moderate cytotoxic activities against SW480 and HL-60 cancer cell lines with IC50 values of 30.6 and 32.9μM, respectively.
A new limonoid, pentandricine (1), along with three known limonoids, ceramicine B (2), 6-de(acetyloxy)-23-oxochisocheton (3), 6-de(acetyloxy)-23-oxo-7-O-deacetylchisocheton (4), have been isolated from the stembark of Chisocheton pentandrus. The chemical structures of the new compound were elucidated on the basis of spectroscopic evidence. All of the compounds were tested for their cytotoxic effects against MCF-7 breast cancer cells. Compounds 1-4 showed weak and no cytotoxicity against MCF-7 breast cancer cells with IC50 values of 369.84, 150.86, 208.93 and 120.09 μM, respectively.
This study aims to improve the larvicidal activity of crude Chisocheton erythrocarpus extract (CCEE) against Aedes aegypti larvae via nano-emulsion formulation method. The fruits of C. erythrocarpus possesses limonoids with good larvicidal activity against Aedes mosquitoes. Using ternary phase diagrams, four selected formulations were prepared using non-ionic surfactants, methylester and water. The optimized nano-emulsion (S5) was discovered to possess submicron particle size (36.32 nm), low polydispersity index (0.348) and higher zeta potential value (-18.2 mV). After 48-hours, the CCEE and S5 demonstrated respective LC50 values of 139.44 ppm and 75.83 ppm against Ae. aegypti larvae. Higher larvicidal activity of S5 signified improved cuticular penetration of the active ingredient. Moreover, morphological deformities of treated larvae indicated possible interference with the larvae's breathing mechanism, contributing to their death. Thus, the nano-emulsion formulation of C. erythrocarpus extract has the potential to be used as a vector control agent to prevent the spread of dengue infections.
Three new limonoids, chisomicines A-C (1-3), have been isolated from the bark of Chisocheton ceramicus. Their structures were determined by 2D NMR, CD spectroscopic methods, and X-ray analysis. Chisomicine A (1) exhibited NO production inhibitory activity in J774.1 cells stimulated by LPS dose-dependently at high cell viability.