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Impact of the Journal of Chemical Information and Modeling Special Issue on Women in Computational Chemistry

Mazzolari A, Nunes-Alves A, Wahab HA, Amaro RE, Cournia Z, Merz KM

J Chem Inf Model, 2020 07 27;60(7):3328-3330.
PMID: 32623887 DOI: 10.1021/acs.jcim.0c00636

In this Viewpoint, we provide a commentary on the impact of the Journal of Chemical Information and Modeling Special Issue on Women in Computational Chemistry published in May 2019 and the feedback we received.

Matched MeSH terms: Restraint, Physical
Alterations in selected serum biochemistry values of free-ranging Bornean leopard cats (Prionailurus bengalensis borneoensis) captured by box traps

Nájera F, Hearn AJ, Ross J, Nathan S, Revuelta L

Vet Res Commun, 2014 Sep;38(3):265-9.
PMID: 24839119 DOI: 10.1007/s11259-014-9605-2

Box-traps for capturing wild cats are widely used by researchers since it is one of the most effective methods for trapping these species. Although they are extensively utilised, the effects on the physiology of trapped felids remain unclear. Researchers frequently make judgements regarding the safety of such capture devices by examination of external injuries but often fail to take into consideration other physiological parameters. To assess the effects of capture events on selected serum biochemistry values of free-ranging Bornean leopard cats (Prionailurus bengalensis borneoensis) six free-ranging leopard cats (four males, two females) were trapped by using box-traps in Sabah, Malaysian Borneo. Blood was collected by jugular venipucture after chemical immobilization with a mixture of tiletamine and zolazepam. Blood was analysed for 17 serum biochemistry parameters. The most consistent and significantly higher value found in both sexes was aspartate aminotransferase (AST), followed by high mean value of alanine aminotransferase (ALT). Both mean values exceeded the upper limit of the reference range for captive leopard cats. These results demonstrate that captured leopard cats by box-traps undergo physical exertion and consequently some type of muscle injury/damage. Researchers and wildlife managers should be aware of the physiological response of trapped felids when using box-traps. Devices that facilitate the prompt removal of leopard cats from the traps would be useful for researchers to avoid further damage while live trapping this species.

Matched MeSH terms: Restraint, Physical/adverse effects; Restraint, Physical/veterinary*
Fulltext Ascorbic acid protects against restraint stress-induced memory deficits in Wistar rats

Kumar RS, Narayanan SN, Nayak S

Clinics (Sao Paulo), 2009;64(12):1211-7.
PMID: 20037710 DOI: 10.1590/S1807-59322009001200012

Chronic stress has been shown to cause oxidative damage in the central nervous system. Although stress-induced impairments in learning and memory have been studied extensively, very few studies have investigated possible ways to prevent their ill effects. The present work was designed to study the protective effects of ascorbic acid in memory loss induced by chronic restraint stress.

Matched MeSH terms: Restraint, Physical/adverse effects; Restraint, Physical/methods
A correlation on ultrasonication with nanocrystalline cellulose characteristics

Mohd Ishak NA, Khalil I, Abdullah FZ, Muhd Julkapli N

Carbohydr Polym, 2020 Oct 15;246:116553.
PMID: 32747237 DOI: 10.1016/j.carbpol.2020.116553

Catalytic ionic liquid hydrolysis of cellulosic material have been considered as a green and highly efficient dissolution process. However, application of a pre-treatment process, i.e; ultrasonication enhances the hydrolysis of cellulose in ionic liquid by providing mechanical force. In this paper, we describe the impact of both chemical and mechanical approaches to produce nanocrytalline cellulose (NCC) with anticipated particle size, and crystallinity with improved yields. The ultrasonication treatment was evaluated in terms of treatment time and vibration amplitude. It was found that the lowest ultrasonication time (5 min) produced the NCC of highest crystallinity (73 %), but the lowest yield (84 %). In contrary, the highest ultrasonication vibration amplitude at 90 % produced NCC with highest crystallinity value (67 %) as well as yields (90 %). It concludes that ultrasonic pre-treatment improves the hydrolysis process of cellulose in ionic liquid with increasing yield and crystallinity of NCC.

Matched MeSH terms: Restraint, Physical
Use of physical restraint: Nurses' knowledge, attitude, intention and practice and influencing factors

Eskandari F, Abdullah KL, Zainal NZ, Wong LP

J Clin Nurs, 2017 Dec;26(23-24):4479-4488.
PMID: 28233363 DOI: 10.1111/jocn.13778

AIMS AND OBJECTIVES: To investigate the knowledge, attitude, intention and practice of nurses towards physical restraint and factors influencing these variables.
BACKGROUND: A literature review showed a lack of studies focused on the intention of nurses regarding physical restraint throughout the world. Considering that very little research on physical restraint use has been carried out in Malaysia, assessment of nurses' knowledge, attitude, intention and practice is necessary before developing a minimising programme in hospitals.
DESIGN: A cross-sectional study was used.
METHODS: A questionnaire to assess the knowledge, attitude, intention and practice was completed by all nurses (n = 309) in twelve wards of a teaching hospital in Kuala Lumpur.
RESULTS: Moderate knowledge and attitude with strong intention to use physical restraint were found among the nurses. Less than half of nurses considered alternatives to physical restraint and most of them did not understand the reasons for the physical restraint. Nurses' academic qualification, read any information source during past year and nurses' work unit showed a significant association with nurses' knowledge. Multiple linear regression analysis found knowledge, attitude and intention were significantly associated with nurses' practice to use physical restraint.
CONCLUSION: This study showed some important misunderstandings of nurses about using physical restraint and strong intention regarding using physical restraint. Findings of this study serve as a supporting reason for importance of educating nurses about the use of physical restraint.
RELEVANCE TO CLINICAL PRACTICE: Exploring the knowledge, attitude, intention and current practice of nurses towards physical restraint is important so that an effective strategy can be formulated to minimise the use of physical restraints in hospitals.

Matched MeSH terms: Restraint, Physical/psychology*
The effect of educational intervention on nurses' knowledge, attitude, intention, practice and incidence rate of physical restraint use

Eskandari F, Abdullah KL, Zainal NZ, Wong LP

Nurse Educ Pract, 2018 Sep;32:52-57.
PMID: 30029085 DOI: 10.1016/j.nepr.2018.07.007

The use of physical restraint exposes patients and staff to negative effects, including death. Therefore, teaching nursing staff to develop the improve knowledge, skills, and attitudes regarding physical restraint has become necessary. A quasi-experimental pre-post design was used to evaluate the effect of educational intervention on nurses' knowledge, attitude, intention, practice and incidence rate of physical restraint in 12 wards of a hospital using a self-reported questionnaire and a restraint order form in Malaysia. The educational intervention, which included a one-day session on minimising physical restraint use in hospital, was presented to 245 nurses. The results showed a significant increase in the mean knowledge, attitude sand practice score and a significant decrease in the mean intention score of nurses to use physical restraint after intervention. There was a statistically significant decrease in the incidence rate of physical restraint use in the wards of the hospital except geriatric-rehabilitation wards after intervention.

Matched MeSH terms: Restraint, Physical/statistics & numerical data*
Awareness, perception and experience on child restraint system (CRS) and its legislation among Malaysian parents with newborns

Ang JY, Lai JM, Hss AS, Ramalingam P, Ramasamy M, Zainuddin NS, et al.

Traffic Inj Prev, 2020;21(4):278-282.
PMID: 32297815 DOI: 10.1080/15389588.2020.1746773

Objective: The aim of this study was to explore parents' awareness and usage of CRS, incentives and challenges in CRS use, as well as their perception about potential CRS legislation in Malaysia.Methods: This was a cross-sectional study conducted in a public tertiary care center in Northern Malaysia. A self-developed researcher-assisted questionnaire was developed in English and translated into Malay language, to explore parents' awareness ad CRS usage, as well as the incentives and barriers in CRS use. Parents of a newborn were conveniently sampled, excluding parents with pre-term newborn or those without a car. Upon obtaining written informed consent, the parents were allowed to answer the questionnaire within 20 minutes, assisted by the researchers. Parents who were found not using CRS for their newborn were given a digital pamphlet with CRS-related information via mobile devices.Results: A total of 200 parents were approached and 193 of them consented to participate in this study. The majority of the parents (87.6%) were aware of CRS, but only half of them (57.0%) provided a CRS for their current newborn. From 100 CRS users, most of them used a CRS for "child's safety" purposes (84.5%). Among 83 CRS nonuser, nearly half of them (45.8%) perceived that it is adequate to carry their child during traveling without using a CRS. When exploring previous experience in using CRS among all parents (n = 193), more than half of them (63.9%) faced difficulty as their child refused to use the CRS. More than half (54.9%) of all parents were not aware about the possible CRS legislation in Malaysia. However, the majority of them (90.7%) agreed with the implementation of CRS law.Conclusions: Although majority of the parents were aware about CRS, but only half had a CRS for their current newborn. Most of the parents supported CRS legislation in Malaysia, but assistance will be required to help them through challenges related to CRS use.

Matched MeSH terms: Restraint, Physical/legislation & jurisprudence*
Incidence Rate and Patterns of Physical Restraint Use Among Adult Patients in Malaysia

Eskandari F, Abdullah KL, Zainal NZ, Wong LP

Clin Nurs Res, 2018 03;27(3):278-295.
PMID: 27856788 DOI: 10.1177/1054773816677807

Incidence rate and patterns of physical restraint use were examined based on a cross-sectional study in 22 wards of a large teaching hospital in Malaysia. Results indicated that the highest rate of physical restraint (19.7%) was reported from neurology-neurosurgery wards. "Un-cooperative for electroconvulsive therapy" and "trying to pull out catheters" were the most commonly reported reasons to use restraint in psychiatric and non-psychiatric wards, respectively. There were some relationships between patterns of physical restraint in this study. Exploring the incidence rate and patterns of physical restraint is important so that effective strategies can be formulated to minimize using restraint in hospitals.

Matched MeSH terms: Restraint, Physical/statistics & numerical data*
Condom-murder

Murty OP

J Forensic Leg Med, 2009 Jan;16(1):35-9.
PMID: 19061848 DOI: 10.1016/j.jflm.2008.05.017

Condom-murder is term used to describe a homicide where a person has been killed due to his recreational sexual behaviour and the killing has strong links with the recovered condom/contraceptive material on the scene or was in personal possession of victim. It can provide immediate clue about perpetrator. We present six cases of a similar nature. Four males and two females had condoms in their vicinity at crime scene. All incidents occurred at different timings and different places but there was striking similarity in method of killing. In four cases hands were tied on the back in three cases; similarly legs were also tied in three cases. In three cases strong electric cords were used. The presence of condoms give clue for investigation, possibility of DNA identification, about type of sexual behaviour as recreational and commercial method in these cases.

Matched MeSH terms: Restraint, Physical
The effect of endogenous opioids on blood pressure during stress

Nordin M, Morat P, Zainora M

Clin Exp Pharmacol Physiol, 1987 Apr;14(4):303-8.
PMID: 3665195

1. A series of experiments were conducted to investigate the effect of endogenous opioids on blood pressure of laboratory rats during stress. 2. Rats subjected to 120 min immobilization showed a significant drop in systolic pressure which could be prevented by pretreatment injections of naloxone. 3. Adrenalectomized rats subjected to the same kind of stress showed a drop in systolic pressure equivalent to only 30% of the systolic pressure drop in the intact animals. This decrease in systolic pressure could also be prevented by pretreatment injections of naloxone. 4. It was concluded that the decrease in systolic pressure in intact rats during immobilization was mostly due to endogenous opioids released from the adrenal glands, whereas opioids of other origins such as the pituitary gland, were also important.

Matched MeSH terms: Restraint, Physical
Fulltext Effect of uv-ozone treatment on physicochemical properties of mesoporous hollow carbon nanoparticles

Muhammad Nur Amir Azman, Yusilawati Ahmad Nor, Nur Husna Samsudin, Ma’an Fahmi Rashid Alkhatib, Yeow, Tshai Kim

Biological and Natural Resources Engineering Journal, 2020;3(2):35-45.
MyJurnal

Carbon nanoparticles have been widely used in various applications. However, they are commonly known to have low dispersibility and chemical inertness which limit their practical ability in medical or biological area. Some studies have been performed to modify carbon nanoparticles such as carbon nanotubes using ultraviolet (UV)-Ozone system. However, little is known on the effects of such system towards other types of carbon nanoparticles such as mesoporous hollow carbon nanoparticles (MHCNs). Thus, in this study, improvement of MHCNs physiochemical properties have been studied using UV-Ozone treatment for the first time. The treatment was conducted in water as dispersant agent at ozone flowrate of 1.0 L/min and exposure time of 45 min. SEM images observed that MHCNs morphology and surface structure remain intact after the treatment. Observations on the dispersibility of MHCNs in phosphate buffered saline (PBS) solution shows that the dispersibility was improved compared to the untreated ones. This was supported by the low Z-average and PDI values of treated MHCNs obtained at ~400 nm and 0.2, respectively when compared to the untreated MHCNs which was obtained at 970 nm and 0.417, respectively. Thermogravimetric analysis (TGA) showed an increased in weight loss of treated MHCNs at the lower temperature compared to untreated MHCNs. Results from Fourier Transform Infrared (FTIR) showed an increase number of new functional groups that includes carboxylic acid group presence at the surface of treated MHCNs which contributes to the improvement of their dispersibility, thermal properties and chemical functionality. These findings opened a new possibility of using UV-Ozone treatment to improve physicochemical properties of MHCNs for medical area such as in drug delivery application in addition to their excellent storage and carrier system.

Matched MeSH terms: Restraint, Physical
Mulberry leaves protect rat tissues from immobilization stress-induced inflammation

Lee CY, Cheng HM, Sim SM

Biofactors, 2007;31(1):25-33.
PMID: 18806306

The ability of the antioxidants in the mulberry leaves to protect Sprague-Dawley rats from injuries caused by immobilization stress was studied as an indicator of the tissue bioavailability of antioxidants. Nitrite level, lipid peroxidation and total antioxidant activity (TAA) in the plasma and tissues were measured. There were hypertrophy of the adrenal glands and kidneys, significant increased levels of nitrite in the plasma and adrenal glands, elevated thiobarbituric acid reactive substances (TBARS) in the plasma, kidneys and spleen, and a reduction of TAA in the plasma, liver, adrenal glands, kidneys and spleen of the immobilized rats. Antioxidants in the mulberry leaf extract suppressed the increase of nitrite and TBARS. Adrenal glands appeared to be the target organ of the antioxidants in the leaf extract. The low dose mulberry antioxidants were more effective than pure rutin (4 mg/day) to protect the cells against inflammation and peroxidation induced by stress.

Matched MeSH terms: Restraint, Physical/adverse effects
Adenosine protects Sprague Dawley rats from high-fat diet and repeated acute restraint stress-induced intestinal inflammation and altered expression of nutrient transporters

Lee CY

J Anim Physiol Anim Nutr (Berl), 2015 Apr;99(2):317-25.
PMID: 25196093 DOI: 10.1111/jpn.12247

This study investigated the effect of repeated acute restraint stress and high-fat diet (HFD) on intestinal expression of nutrient transporters, concomitant to intestinal inflammation. The ability of adenosine to reverse any change was examined. Six-week-old male Sprague Dawley rats were divided into eight groups: control or non-stressed (C), rats exposed to restraint stress for 6 h per day for 14 days (S), control rats fed with HFD (CHF) and restraint-stressed rats fed with HFD (SHF); four additional groups received the same treatments and were also given 50 mg/l adenosine dissolved in drinking water. Fasting blood glucose, plasma insulin, adiponectin and corticosterone were measured. Intestinal expression of SLC5A1, SLC2A2, NPC1L1 and TNF-α was analysed. Histological evaluation was conducted to observe for morphological and anatomical changes in the intestinal tissues. Results showed that HFD feeding increased glucose and insulin levels, and repeated acute restraint stress raised the corticosterone level by 22%. Exposure to both stress and HFD caused a further increase in corticosterone to 41%, while decreasing plasma adiponectin level. Restraint stress altered intestinal expression of SLC5A1, SLC2A2 and NPC1L1. These changes were enhanced in SHF rats. Adenosine was found to alleviate HFD-induced increase in glucose and insulin levels, suppress elevation of corticosterone in S rats and improve the altered nutrient transporters expression profiles. It also prevented upregulation of TNF-α in the intestine of SHF rats. In summary, a combination of stress and HFD exaggerated stress- and HFD-induced pathophysiological changes in the intestine, and biochemical parameters related to obesity. Adenosine attenuated the elevation of corticosterone and altered expression of SLC5A1, NPC1L1 and TNF-α.

Matched MeSH terms: Restraint, Physical/adverse effects*
Effect of honey on the reproductive system of male rat offspring exposed to prenatal restraint stress

Haron MN, Mohamed M

Andrologia, 2016 Jun;48(5):525-31.
PMID: 26289766 DOI: 10.1111/and.12473

Exposure to prenatal stress is associated with impaired reproductive function in male rat offspring. Honey is traditionally used by the Malays for enhancement of fertility. The aim of this study was to determine the effect of honey on reproductive system of male rat offspring exposed to prenatal restraint stress. Dams were divided into four groups (n = 10/group): control, honey, stress and honey + stress groups. Dams from honey and honey + stress groups received oral honey (1.2 g kg(-1) body weight) daily from day 1 of pregnancy, meanwhile dams from stress and honey + stress groups were subjected to restraint stress (three times per day) from day 11 of pregnancy until delivery. At 10 weeks old, each male rat offspring was mated with a regular oestrus cycle female. Male sexual behaviour and reproductive performance were evaluated. Then, male rats were euthanised for assessment on reproductive parameters. Honey supplementation during prenatal restraint stress significantly increased testis and epididymis weights as well as improved the percentages of abnormal spermatozoa and sperm motility in male rat offspring. In conclusion, this study might suggest that supplementation of honey during pregnancy seems to reduce the adverse effects of restraint stress on reproductive organs weight and sperm parameters in male rat offspring.

Matched MeSH terms: Restraint, Physical/adverse effects*
Fulltext Effects of Tualang honey on pain behaviour and oxidative stress in the thalamus of prenatally stressed rat offspring

Hidani Hasim, Che Badariah Abd Aziz, Siti Qusyasyiah Ahmad Suhaimi, Mahaneem Mohamed, Idris Long, Rahimah Zakaria

Malaysian Journal of Medicine and Health Sciences, 2020;16(3):85-92.
MyJurnal

Introduction: Increased nociceptive responses were shown in the offspring of prenatally stressed rats. Reports have demonstrated the anti-nociceptive effects of Tualang honey in the rat offspring. The present study was done to de- termine whether the modulation of nociceptive behaviour by Tualang honey was mediated by modulating changes in the histology, oxidative stress parameters and N-methyl-D-aspartate (NMDA) receptors in the thalamus of the rat offspring. Methods: Eighteen Sprague Dawley pregnant rats were randomly assigned to control (C), stress (S) and stress-treated with Tualang honey (SH) groups. Stress was given in a form of restraint stress.Tualang honey was given to SH group from first day of pregnancy until delivery. Thirty-three adult male offspring were subjected to formalin test before they were sacrificed. Nociceptive behaviour score, number of neurons, level of oxidative stress parameters and NMDA receptors in the thalamus were analysed by using one-way ANOVA. Results: The study demonstrated a significant decrease in mean nociceptive behaviour score (p

Matched MeSH terms: Restraint, Physical
The effect of melatonin on delirium in hospitalised patients: A systematic review and meta-analyses with trial sequential analysis

Ng KT, Teoh WY, Khor AJ

J Clin Anesth, 2020 Feb;59:74-81.
PMID: 31279283 DOI: 10.1016/j.jclinane.2019.06.027

OBJECTIVES: Melatonin is an endogenous hormone, which regulates circadian rhythms and promotes sleep. In recent years, several randomised controlled trials examining the prophylactic use of melatonin to prevent delirium were published with conflicting findings. The primary aim of this review was to determine the effect of melatonin on the incidence of delirium in hospitalised patients.
DATA SOURCES: MEDLINE, EMBASE and CENTRAL were systematically searched from their inception until December 2018.
REVIEW METHODS: All randomised clinical trials were included.
RESULTS: Sixteen trials (1634 patients) were included in this meta-analysis. Incidence of delirium was not significantly lower in patients who received melatonin, with an odd ratio, OR (95%Cl) of 0.55 (0.24-1.26); ρ = 0.16, certainty of evidence = low, trial sequential analysis = inconclusive. However, patients who randomised to melatonin had a significantly shorter length of stay in intensive care units, with a mean difference, MD (95%CI) of -1.84 days (-2.46, -1.21); ρ restraints (OR 95%Cl 0.65; 0.31-1.37; ρ = 0.26) and the requirement of sedative agents (OR 95%Cl 0.86; 0.48-1.55; ρ = 0.62).
CONCLUSIONS: In summary, the results of this meta-analysis of sixteen trials neither support nor oppose the use of melatonin in the prevention of delirium of hospitalised patients. We identified high heterogeneity across all the included trials and low certainty of evidence with potential type II error. Future multi-centre, adequately powered randomised controlled trials are warranted to provide more certainty on the use of melatonin for the prevention of delirium.
PROSPERO: CRD42019123546.

Matched MeSH terms: Restraint, Physical/statistics & numerical data
Conformational properties of serum albumin binding sites in rats with different behaviour in the open field test

Gryzunov YA, Koplik EV, Smolina NV, Kopaeva LB, Dobretsov GE, Sudakov KV

Stress, 2006 Mar;9(1):53-60.
PMID: 16753933

In this study, the hypothesis was tested that behaviour of rats under the open field test condition and effects of subsequent acute stress relate to conformational properties of the main plasma carrier protein, albumin.To evaluate albumin properties, fluorescence intensity of a molecular probe CAPIDAN (N-carboxyphenylimide of dimethylaminonaphthalic acid) at N (at pH 7.4) and F (at pH 4.2) albumin conformations was measured and the N-F signal ratio was calculated. The data obtained showed that CAPIDAN fluoresces selectively from albumin in rat serum and its fluorescence is sensitive to binding of fatty acids and some other ligands to albumin. Behaviour of 78 Wistar male rats was characterized from the fraction of time taken for exploratory and ambulatory activity during the open field test. In rats not subjected to stress (n = 40), a negative correlation was revealed between open field activity and CAPIDAN N-to-F ratio for albumin (r = - 0.55, p < 0.0005). In the group of rats subjected to acute stress (immobilization plus stochastic electrocutaneous stimulation) the correlation between behavioural activity and the albumin conformational properties was significantly positive (r = 0.59, p < 0.0001): the CAPIDAN albumin fluorescence ratio increased in the highly active rats and decreased in the low-activity rats. The mechanisms of the observed effects may involve differences in nonesterified fatty acid production during stress.

Matched MeSH terms: Restraint, Physical
Chronic restraint stress induces intestinal inflammation and alters the expression of hexose and lipid transporters

Lee CY

Clin Exp Pharmacol Physiol, 2013 Jun;40(6):385-91.
PMID: 23586523 DOI: 10.1111/1440-1681.12096

Psychosocial stress is reported to be one of the main causes of obesity. Based on observations in studies that relate stress and gut inflammation to obesity, the present study hypothesized that chronic stress, via inflammation, alters the expression of nutrient transporters and contributes to the development of metabolic syndrome. Rats were exposed to restraint stress for 4 h/day for 5 days/week for eight consecutive weeks. Different segments of rat intestine were then collected and analysed for signs of pathophysiological changes and the expression of Niemann-Pick C1-like-1 (NPC1L1), sodium-dependent glucose transporter-1 (SLC5A1, previously known as SGLT1) and facilitative glucose transporter-2 (SLC2A2, previously known as GLUT2). In a separate experiment, the total anti-oxidant activity (TAA)-time profile of control isolated intestinal segments was measured. Stress decreased the expression of NPC1L1 in the ileum and upregulated SLC5A1 in both the jejunum and ileum and SLC2A2 in the duodenum. Inflammation and morphological changes were observed in the proximal region of the intestine of stressed animals. Compared with jejunal and ileal segments, the rate of increase in TAA was higher in the duodenum, indicating that the segment contained less anti-oxidants; anti-oxidants may function to protect the tissues. In conclusion, stress alters the expression of hexose and lipid transporters in the gut. The site-specific increase in the expression of SLC5A1 and SLC2A2 may be correlated with pathological changes in the intestine. The ileum may be protected, in part, by gut anti-oxidants. Collectively, the data suggest that apart from causing inflammation, chronic stress may promote sugar uptake and contribute to hyperglycaemia.

Matched MeSH terms: Restraint, Physical
Immunohistochemical characteristics of the hypophysis in normal conditions and chronic stress

Kapitonova MY, Kuznetsov SL, Khlebnikov VV, Zagrebin VL, Morozova ZCh, Degtyar YV

Neurosci. Behav. Physiol., 2010 Jan;40(1):97-102.
PMID: 20012496 DOI: 10.1007/s11055-009-9217-4

Quantitative immunohistochemical methods were used to assess activation of the hypothalamo-hypophyseal-adrenocortical system at the level of its central component - the adenohypophysis - in the growing body during chronic exposure to psychoemotional stressors of different strengths. Sprague-Dawley rats aged 30 days were subjected to "mild" or "severe" immobilization stress for 5 h per day for seven days. Animals were decapitated at the end of the last stress session and the endocrine glands (hypophysis, adrenals) were harvested, weighed, and embedded in paraffin; sections were stained with hematoxylin and eosin, and also immunohistochemically using monoclonal antibodies to adrenocorticotropic hormone (ACTH) and proliferating cell nuclear antigen (PCNA) following by automated image analysis. These studies showed that stress-associated hyperplasia of corticotropocytes in rats of pubertal age was due more to the differentiation of existing immature precursor cells than to cell proliferation.

Matched MeSH terms: Restraint, Physical
Fulltext Tocotrienol and alpha-tocopherol reduce corticosterone and noradrenalin levels in rats exposed to restraint stress

Nur Azlina MF, Nafeeza MI

Pharmazie, 2008 Dec;63(12):890-2.
PMID: 19177905

This study investigates the effects of tocotrienol (TT) or alpha-tocopherol (TF) supplementation on corticosterone level, noradrenalin level and gastric lesions in rats exposed to restraint stress. Twenty-four male Sprague Dawley rats were randomly assigned into 4 equally sized groups; two control groups were given olive oil, while the treated group was supplemented with either tocotrienol of tocopherol orally at a dose of 60 mg/kg body weight. After 28 days of treatment, one control group, TT group and TF group were subjected to restraint stress, 2 hours daily for 4 consecutive days. After the last exposure to stress, plasma samples were taken to determine the corticosterone and noradrenalin levels, after which the rats were sacrificed. The stomach was excised for the evaluation of gastric lesions. Our findings showed that TT and TF were able to maintain the corticosterone level to the prestress values, while only TT was able to maintain the noradrenalin level in rats exposed to stress. Tocotrienol was found to be better in preventing formation of gastric lesions compared to TF. As a conclusion, the protective effect of vitamin E was related to the ability to inhibit stress induced elevation of corticosterone and noradrenalin levels.

Matched MeSH terms: Restraint, Physical

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