Browse publications by year: 1999

  1. Suraya Y, Saw KC
    Singapore Med J, 1999 Oct;40(10):656-8.
    PMID: 10741196
    Cases of genital self-mutilation are usually seen in the general hospital setting and can be difficult to manage especially in those patients who have psychiatric illness. A joint effort between the psychiatric and the surgical services will be required right from the beginning of hospital admission to diagnosis and later, to follow-up. Psychiatric consultation strategies at the different phases of intervention will be needed to cater for the special needs of the surgical team, patient and family. We describe three cases of genital self- mutilators and the general management of these patients.
    MeSH terms: Adult; Aged; Mental Disorders/complications*; Family Health; Genitalia, Male/injuries*; Genitalia, Male/surgery; Humans; Interprofessional Relations; Male; Middle Aged; Penis/injuries; Penis/surgery; Self Mutilation/psychology*; Self Mutilation/surgery; Self Mutilation/therapy
  2. Lian PC, Marnoch G
    J Manag Med, 1999;13(2-3):178-89.
    PMID: 10747449
    This paper describes a study of the knowledge and attitudes of a sample of private medical practitioners in Malaysia in relation to questions posed on guidelines-based medicine. The study was conducted through a postal questionnaire sent to a sample of 2,000 private practitioners. Placing the research in context, a description of Malaysia and its health-care system is provided. Issues surrounding guidelines-based medicine in the Malaysian context are discussed. An analysis of the sample population's personal and professional characteristics is presented. Key research findings include identifying a substantial knowledge deficit--less than half of the respondents claimed to understand "guidelines". A majority of doctors (64 per cent) think guidelines are useful. Only 29 per cent of respondents knew where to find guidelines, with 22 per cent claiming to have used guidelines. The paper concludes with a discussion of issues in the implementation of guidelines-based medicine in Malaysia based on the study's findings.
    MeSH terms: Attitude of Health Personnel*; Delivery of Health Care/organization & administration; Delivery of Health Care/standards; Developing Countries; Humans; Information Services; Health Knowledge, Attitudes, Practice*; Malaysia; Physicians/psychology*; Physicians/statistics & numerical data; Private Practice; Surveys and Questionnaires; Cluster Analysis; Practice Guidelines as Topic*; Evidence-Based Medicine/statistics & numerical data*
  3. Ong ST, Ngeow WC
    Dent Update, 1999 May;26(4):163-5.
    PMID: 10765768
    Sinus on the chin can be the result of a chronic apical abscess due to pulp necrosis of a mandibular anterior tooth. The tooth is usually asymptomatic, and a dental cause is therefore not apparent to the patient or the unsuspecting clinician. Not infrequently, the patient may seek treatment from a dermatologist or general surgeon instead of a dentist. Excision and repair of the fistula may be carried out with subsequent breakdown because the dental pathology is not removed. This paper reports the presence of median mental sinus of dental origin in twins. One case healed following root canal therapy while the other required both root canal therapy and surgery to eliminate the infection.
    MeSH terms: Adolescent; Chin*; Dental Fistula/etiology*; Dental Fistula/therapy; Female; Humans; Periodontal Abscess/complications*; Periodontal Abscess/therapy; Root Canal Therapy; Cutaneous Fistula/etiology*; Cutaneous Fistula/therapy
  4. Chai WL, Ngeow WC
    Dent Update, 1999 Sep;26(7):298-302.
    PMID: 10765775
    Hypodontia is the congenital absence of one or more teeth because of agenesis. The most commonly missing teeth are the third molars, the maxillary lateral incisors and the second premolars. Cases are presented of three patients with a missing mandibular incisor.
    MeSH terms: Adolescent; Adult; Anodontia/complications; Anodontia/genetics*; Anodontia/therapy; Family Health; Female; Humans; Incisor/abnormalities*; Male; Mandible; Tooth Eruption, Ectopic/complications; Tooth, Impacted/complications; Orthodontic Space Closure
  5. Lui JL
    Quintessence Int, 1999 Sep;30(9):601-6.
    PMID: 10765865
    The introduction of an intraradicular composite reinforcing technique, in conjunction with the reestablishment of matching post canal spaces, has allowed compromised, root-filled teeth to be restored with functional, esthetic post crowns. This clinical case report suggests that reconstituted post canals, in accurately adapting to passive, parallel-sided, matching, and well-fitting posts, can enhance the retention of post crowns. Other factors of clinical importance relating to the resin-reinforced technique are discussed, including fracture resistance, depth of polymerization, dentin adhesion, polymerization shrinkage, and coronal microleakage.
    MeSH terms: Adult; Composite Resins; Crowns; Glass Ionomer Cements; Humans; Incisor/injuries; Male; Post and Core Technique*; Tooth Fractures/therapy; Dental Prosthesis Retention*; Dental Marginal Adaptation; Retreatment; Tooth, Nonvital/therapy*
  6. Chang MS, Matusop A, Sen FK
    PMID: 10774651
    Anopheles mosquitos were surveyed using three trapping technics in four longhouse settlements and their respectively farming zone in western Sarawak, Malaysia. The study area was mountainous with tropical rain forest. An. leucosphyrus and An. donaldi were predominant in the farm huts. An. tessellatus and An. subpictus were more abundant in the village settlements. In both ecotypes, human baited traps yielded a significantly greater proportion of Anopheles mosquito than CDC light traps and landing biting catches. Circumsporozoite antigen positively rate, mosquito survival rate and parasite rate showed that malaria transmission is more intense in farm huts than in longhouse settlements. The entomological inoculation rate of An. donaldi and An. leucosphyrus in farm huts was 0.035 and 0.023, respectively. No sporozoite infections were observed in the main settlements.
    MeSH terms: Analysis of Variance; Animals; Enzyme-Linked Immunosorbent Assay; Humans; Insect Vectors*; Malaysia/epidemiology; Plasmodium falciparum/isolation & purification; Plasmodium vivax/isolation & purification; Population Density; Rural Health; Incidence; Prevalence; Chi-Square Distribution; Malaria, Falciparum/transmission*; Malaria, Vivax/transmission*
  7. Lee ASC, Yap KL
    PMID: 10774695
    Poliovirus kept on the cut surfaces of fully ripe papaya cubes placed in an ice box showed a sharp and significant reduction in the recovery of infectious virus about 15 minutes after exposure. Thereafter, a very gradual decrease ensued and infectious residual virus was detected up to the end of the 6-hour exposure period. Papaya cubes washed or kept overnight before virus inoculation, and from less ripe fruits produced a similar survival pattern. A very small proportion of the inoculum was recovered from the mashed content of the inoculated papaya cubes thus suggesting that most of the non-recovered virus particles were inactivated. The results suggest that the importance of poliovirus-contaminated cut papayas as a transmission vehicle for the virus is greatly reduced by the rapid decline in the infectivity of a large proportion of the virus soon after contamination. Nevertheless, the potential to transmit remains as a small residual pool of infectious poliovirus is able to survive for a relatively long period.
    MeSH terms: Cells, Cultured; Food Handling*; Food Microbiology*; Fruit/virology*; Humans; Malaysia; Poliomyelitis/prevention & control; Poliomyelitis/transmission*; Refrigeration; Poliovirus/growth & development*
  8. Sheffield J, Hussain A, Coleshill P
    J Manag Med, 1999;13(4-5):263-4.
    PMID: 10787497
    The NHS faces a crisis in terms of staffing and recruitment. Many of the ethnic minority GPs in inner city areas throughout the UK are coming up to retirement age, and there is an insufficient supply of trainees to fill estimated vacancies. Over 2,000 nursing vacancies exist across the UK, and recruitment to the profession and retention within the profession are poor. Nurses have been recruited from overseas for the past 40 years, and are currently being recruited from Finland, Malaysia, and the West Indies, whilst doctors are being sought in India, Pakistan and Africa. Overseas recruitment is not a new phenomenon, and numerous studies have been carried out to examine equal opportunities and racial discrimination within the NHS. The aim of this paper was to examine ethnicity and equal opportunities within the Scottish NHS and record the levels of organisational awareness of ethnicity and equal opportunities' issues. The paper also examines the link between health service delivery to ethnic minorities and internal cultural attitudes to staff.
    MeSH terms: Career Mobility; Health Services Accessibility; Humans; Minority Groups*; Prejudice*; Scotland; State Medicine/organization & administration*
  9. Shimizu H, Utama A, Yoshii K, Yoshida H, Yoneyama T, Sinniah M, et al.
    Jpn J Infect Dis, 1999 Feb;52(1):12-5.
    PMID: 10808253
    Enterovirus 71 (EV71), one of the major causative agents for hand, foot and mouth disease (HFMD), is sometimes associated with severe central nervous system diseases. In 1997, in Malaysia and Japan, and in 1998 in Taiwan, there were HFMD epidemics involving sudden deaths among young children, and EV71 was isolated from the HFMD patients, including the fatal cases. The nucleotide sequences of each EV71 isolate were determined and compared by phylogenetical analysis. EV71 strains from previously reported epidemics belonged to genotype A-1, while those from recent epidemics could be divided into two genotypes, A-2 and B. In Malaysia, genotype A-2 was more prevalent, while in Japan and Taiwan, B genotype was more prevalent. Two isolates from fatal cases in Malaysia and one isolate from a fatal case in Japan were genotype A-2. However, all isolates from three fatal cases in Taiwan belonged to genotype B. The severity of the HFMD did not link directly to certain genotypes of EV71.
    MeSH terms: Disease Outbreaks*; Enterovirus/classification; Enterovirus/genetics; Enterovirus/isolation & purification*; Genotype; Hand, Foot and Mouth Disease/epidemiology*; Hand, Foot and Mouth Disease/virology*; Humans; Infant; Infant, Newborn; Japan/epidemiology; Malaysia/epidemiology; Phylogeny; Severity of Illness Index; Taiwan/epidemiology; DNA Primers; Reverse Transcriptase Polymerase Chain Reaction
  10. Inayat-Hussain SH, Cohen GM, Cain K
    Cell Biol Toxicol, 1999;15(6):381-7.
    PMID: 10811533
    There is now a wealth of information regarding the apoptotic mode of cell death and its importance in toxicological studies in many mammalian organs including the liver. In this study, we investigated the modulatory effects of the heavy metal Zn2+ on transforming growth factor-beta1 (TGF-beta1)-induced apoptosis in primary rat hepatocytes. Apoptosis induced by TGF-beta1 (1 ng/ml) in hepatocytes was accompanied by nuclear condensation as assessed morphologically by staining with Hoechst 33258 and DNA cleavage as detected biochemically by in situ end-labeling, field inversion and conventional gel electrophoresis. Pretreatment with 100 micromol/L Zn2+ abrogated the nuclear condensation, in situ end-labeling, and DNA laddering in TGF-beta1-treated hepatocytes. Surprisingly, Zn2+ did not inhibit the formation of high-molecular-weight DNA fragments (30-50 kbp to 250-300 kbp). These data provide evidence that Zn2+ exerts its effects on the endonucleases that act downstream in the execution phase of TGF-beta1-induced apoptosis in hepatocytes.
    MeSH terms: Animals; Cells, Cultured; Chromatin/drug effects; DNA/drug effects; Liver/cytology; Liver/drug effects*; Male; Rats, Inbred F344; Zinc/toxicity*; Transforming Growth Factor beta/physiology*; Apoptosis/drug effects; Apoptosis/physiology*; Rats
  11. Mafauzy M, Mokhtar N, Mohamad WB, Musalmah M
    Asia Pac J Public Health, 1999;11(1):16-9.
    PMID: 10829822 DOI: 10.1177/101053959901100104
    Two thousand five hundred and eight subjects from the state of Kelantan in North-East Peninsular Malaysia were included in this study to determine the prevalence of diabetes mellitus and impaired glucose tolerance and their association with cardiovascular risk factors. The overall prevalence of diabetes mellitus was 10.5% and impaired glucose tolerance was 16.5%. There was no difference in the prevalence of diabetes mellitus between males and females but the prevalence of impaired glucose tolerance was higher in females (19.0%) than in males (11.5%). Subjects with diabetes mellitus were more obese (38.4%) than normal subjects (24.1%). They also had a higher prevalence of hypertension (12.9%) and hypercholesterolaemia (71.9%) than normal subjects. Subjects with impaired glucose tolerance also had a higher prevalence of obesity (35.5%), hypertension (9.0%) and hypercholesterolaemia (63.0%) than normal subjects. In conclusion, the prevalence of diabetes mellitus and impaired glucose tolerance was high and they were associated with a high prevalence of obesity, hypertension and hypercholesterolaemia.
    MeSH terms: Adult; Aged; Cardiovascular Diseases/epidemiology*; Diabetes Mellitus/epidemiology*; Female; Health Surveys; Humans; Malaysia; Malaysia/epidemiology; Male; Middle Aged; Prediabetic State; Prevalence; Risk Assessment/statistics & numerical data
  12. Sinoway BA, Baxter DR, Santa Maria R
    J Health Hum Serv Adm, 1999;22(1):33-6.
    PMID: 10848192
    MeSH terms: Adult; Burnout, Professional/epidemiology; Burnout, Professional/psychology*; Humans; Job Satisfaction; Malaysia/epidemiology; Models, Psychological*; Surveys and Questionnaires
  13. Abdullah J, Isa MN
    Stereotact Funct Neurosurg, 1999;73(1-4):19-22.
    PMID: 10853092
    Two hundred primary brain tumours in both adults and children from the year 1990 to 1998 presenting for treatment to the Neurosurgical Division of the Hospital of the University of Sciences Malaysia were studied retrospectively. Volumes of tumours were taken from CT scans with contrast using two formulas and divided into 4 groups: (1) less than 20 cm(3), (2) 20-50 cm(3), (3) 50-100 cm(3) (4) larger than 100 cm(3). The majority of the brain tumours were in the volume range of 50-100 cm(3), and are thus potentially curable with retroviral gene therapy.
    MeSH terms: Adult; Brain Neoplasms/pathology; Brain Neoplasms/surgery; Brain Neoplasms/therapy*; Child; Humans; Malaysia; Retrospective Studies; Genetic Therapy*
  14. Abdullah J, Rushdan A, Hamzah M, Ariff AR, Rani A
    Ann. Transplant., 1999;4(3-4):28-31.
    PMID: 10853777
    Four patients underwent reconstruction of anterior cranial fossa of skull defects between November 1997 and January 1998. All of them had traumatic anterior cranial fossa defect and were reconstructed with lyophilised bovine cortical bone graft. There was no cerebrospinal fluid leakage, meningitis, extradural abscess or other infections. This study demonstrates that the use of bovine bone graft in the reconstruction of anterior base defects is safe.
    MeSH terms: Adolescent; Adult; Animals; Cattle; Humans; Middle Aged; Surgical Wound Infection/prevention & control; Tomography, X-Ray Computed; Transplantation, Heterologous; Bone Transplantation/methods*; Skull Base/injuries; Skull Base/radiography; Skull Base/surgery*
  15. Ng KP, Saw TL, Madasamy M, Soo Hoo T
    Mycopathologia, 1999;147(1):29-32.
    PMID: 10872513
    The common etiological agents of onychomycosis are dermatophytes, molds and yeasts. A mycological nail investigation of onychomycosis using direct microscopy and culture was conducted by the Mycology Unit, Department of Medical Microbiology, University of Malaya from March 1996 to November 1998. The study involved 878 nail clippings or subungal scrapings from subjects with onychomycosis. On direct microscopy examination, 50% of the specimens were negative for fungal elements. On culture, 373 specimens had no growth; bacteria were isolated from 15 nail specimens. Among the 490 specimens with positive fungal cultures, 177 (36.1%) were dermatophytes, 173 (35.5%) were molds and 130 (26.5%) were Candida. There were 2% (10/490) mixed infections of molds, yeasts and dermatophytes. Trichophyton rubrum (115/177) and Trichophyton mentagrophytes (59/177) were the main dermatophytes isolated. The molds isolated were predominantly Aspergillus niger (61/173), Aspergillus nidulans (30/173), Hendersonula toruloidea (26/173) and Fusarium species (16/173). 96.9% of the Candida species identified were Candida albicans.
    MeSH terms: Aspergillosis/microbiology; Aspergillosis/epidemiology; Aspergillus nidulans/growth & development; Aspergillus nidulans/pathogenicity; Aspergillus niger/growth & development; Aspergillus niger/pathogenicity; Candida/growth & development*; Candida/pathogenicity; Candidiasis/microbiology*; Candidiasis/epidemiology; Fungi/growth & development*; Fungi/pathogenicity; Fusarium/growth & development; Fusarium/pathogenicity; Humans; Malaysia/epidemiology; Prospective Studies; Tinea/microbiology; Tinea/epidemiology; Onychomycosis/microbiology*; Onychomycosis/epidemiology*; Trichophyton/growth & development*; Trichophyton/pathogenicity; Colony Count, Microbial
  16. Jernigan DH
    J Public Health Policy, 1999;20(1):56-80.
    PMID: 10874398
    As evidence emerges showing alcohol's significant share of the global burden of disease, alcohol sales have flattened in the developed countries, but sales are rising in developing and post-communist countries. A three-year study sought to assess the growing impact of global alcohol transnationals in the developing and post-communist countries. Case studies in three countries--Malaysia, Zimbabwe, and Estonia--provide concrete examples of current global alcohol marketing policies and procedures. Recommendations stress the need for national and local governments, international bodies, non-governmental organizations, and the global alcohol companies to adopt specific measures designed to achieve improved monitoring of alcohol problems, greater public awareness of alcohol's impact, stronger and more effective regulation of the alcohol trade, and greater restraint on the part of the companies. Alcohol problems are too serious and too preventable for the world to be left thirsting for action.
    MeSH terms: Alcohol Drinking*; Commerce/methods*; Estonia; Malaysia; Public Health; Research; Zimbabwe
  17. Cheah PL, Looi LM
    Malays J Pathol, 1999 Jun;21(1):1-15.
    PMID: 10879274
    Since its recognition about 150 years ago, there has been much progress in the understanding of the pathogenesis, prevention, early detection and management of carcinoma of the uterine cervix. Important historical landmarks include the (1) recognition of pre-invasive and pre-clinical lesions, and the devise of various systems for reporting these lesions, (2) improvements in diagnostic techniques particularly colposcopy, (3) advent of therapeutic procedures (electrocoagulation, cryotherapy, laser therapy and loop electrosurgical excision), and (4) recognition of the aetiological relationship between the human papillomavirus and cervical neoplasia. The susceptibility of the cervical transformation zone to malignant change is now well recognised. The WHO classification system remains the one most commonly utilised for histological reporting of cervical cancers. In the recent 1994 update, cervical carcinoma is divided into 3 main categories: squamous cell carcinoma, adenocarcinoma and other epithelial tumours. Squamous cell carcinoma (60-80%) predominates among invasive cervical carcinoma. Recognised variants include verrucous, warty (condylomatous), papillary squamous (transitional) and lymphoepithelioma-like carcinoma. Adenocarcinoma (5-15% of invasive carcinomas) shows an increasing trend in young females. Like its squamous counterpart, preinvasive and microinvasive versions are known. Variants such as mucinous, endometrioid, clear cell, mesonephric, serous, villoglandular and minimal deviation carcinoma are now defined. Adenosquamous carcinoma (5-25%), adenoid-cystic, adenoid-basal, neuroendocrine and undifferentiated carcinomas constitute other epithelial tumours of the cervix. The management of invasive cervical carcinoma remains heavily dependent on its stage. The FIGO staging system remains the most widely used. The 1995 update provides more definite criteria in subdividing stage IA tumours by delimiting stromal invasion of stage IA1 lesions to a maximum depth of 3 mm and a horizontal axis of 7 mm. In Malaysia, an appreciation of the cervical carcinoma problem has to take into consideration the population at risk, its multi-ethnicity, its socio-economic and geographical diversities and the constraints of the health care system. Females form 48.9% of the Malaysian population. 52.9% of them are in the sexually active age group of 15-50 years, indicating a significant population at risk for cervical carcinoma. Cervical carcinoma was the third most common cause of death due to solid tumours among Malaysian females in 1995 following carcinoma of the breast and respiratory tract. East Malaysia is predominantly rural with many communities having limited modern facilities. Such areas imply a lower educational and socio-economic status, raising the worry of a population at higher risk for developing cervical carcinoma. The population: doctor for Malaysia of 2153:1 compares poorly with nearby Singapore. Besides a shortage of doctors, there is also an uneven distribution of doctors, resulting in a ratio in East Malaysia of > 4000:1. Although Malaysia does not have a national cervical cancer-screening programme, many action plans and cancer awareness campaigns have been launched throughout the years, which appear to have made an impact as evidenced by the decreasing mortality rates from cervical carcinoma. Another interesting feature of cervical carcinoma in Malaysia relates to its multiethnic population. In Malaysian Chinese and Malay females, the prevalence of cervical carcinoma ranks second to breast cancer whereas the pattern is reversed in Malaysian Indian females. Studies into its aetiology and pathogenesis are being undertaken and may shed more light on this matter.
    MeSH terms: Carcinoma/classification; Carcinoma/pathology*; Uterine Cervical Neoplasms/classification; Uterine Cervical Neoplasms/pathology*; Female; Humans; Malaysia; Neoplasm Staging
  18. Jayaram G, Razak A, Gan SK, Alhady SF
    Malays J Pathol, 1999 Jun;21(1):17-27.
    PMID: 10879275
    1853 thyroid lesions subjected to cytological sampling (either by the fine needle aspiration or fine needle capillary sampling technique) from January 1992 to December 1997 at the University Hospital, Kuala Lumpur, were reviewed. Nodular goitre was the most common thyroid lesion needled (67.35%). Among the neoplastic lesions, follicular neoplasms predominated (64%), followed by papillary carcinoma (29.4%). In 325 cases, partial or total thyroidectomy had been done, providing material for histological review and cyto-histological correlation. Cytological diagnosis was found to have high sensitivity and specificity rates of over 75%. Besides, most non-neoplastic thyroid lesions could be diagnosed on cytology. The scope of cytology in the diagnosis of lymphomas, anaplastic and metastatic tumours rendered diagnostic biopsies (or thyroidectomy) unnecessary in these cases. Being a cost-effective technique and having the capacity to provide exact morphological diagnosis in a large variety of thyroid lesions, cytology is obviously the method of choice in the assessment of thyroid nodules.
    MeSH terms: Biopsy, Needle; Goiter, Nodular/pathology; Humans; Sensitivity and Specificity; Thyroid Diseases/pathology; Thyroid Gland/pathology*; Thyroid Neoplasms/pathology
  19. Looi LM
    Malays J Pathol, 1999 Jun;21(1):29-35.
    PMID: 10879276
    A review of routine histopathological samples and autopsies examined at the Department of Pathology, University of Malaya revealed 15 cases of amyloidosis of the lung. Two were localized depositions limited to the lung while in the remainder, lung involvement was part of the picture of systemic amyloidosis. Both cases of localized amyloidosis presented with symptomatic lung/bronchial masses and a clinical diagnosis of tumour. Histology revealed "amyloidomas" associated with heavy plasma cell and lymphocytic infiltration and the presence of multinucleated giant cells. In both cases, the amyloid deposits were immunopositive for lambda light chains and negative for kappa chains and AA protein. One was a known systemic lupus erythematosus patient with polyclonal hypergammaglobulinaemia. The other patient was found to have plasma cell dyscrasia with monoclonal IgG lambda gammopathy. Both patients did not develop systemic amyloidosis. In contrast, lung involvement in systemic AA amyloidosis was not obvious clinically or macroscopically but was histologically evident in 75% of cases subjected to autopsy. Amyloid was detected mainly in the walls of arterioles and small vessels, and along the alveolar septa. It was less frequently detected in the pleura, along the basement membrane of the bronchial epithelium and around bronchial glands. In one case of systemic AL amyloidosis associated with multiple myeloma, an "amyloidoma" occurred in the subpleural region reminiscent of localized amyloidosis. These cases pose questions on (1) whether localized "tumour-like" amyloidosis is a forme fruste of systemic AL amyloidosis and (2) the differing pattern of tissue deposition of different chemical types of amyloid fibrils, with the suggestion that light chain amyloid has a greater tendency to nodular deposition than AA amyloid.
    MeSH terms: Adult; Amyloid/metabolism*; Amyloidosis/metabolism*; Female; Humans; Lung/metabolism*; Male; Middle Aged; Tissue Distribution
  20. Tay SP, Cheong SK, Hamidah NH, Ainoon O
    Malays J Pathol, 1999 Jun;21(1):37-43.
    PMID: 10879277
    A comparative study was conducted to evaluate three different permeabilization methods: FACS Permeabilizing Solution (FPerm), CytoFix/CytoPerm Kit (CFP) and Paraformaldehyde-Tween 20 (PFT) reagents, in cytoplasmic labeling of myeloperoxidase (MPO). Peripheral blood cells from 23 healthy subjects were fixed and permeabilized according to the proposed procedures, prior to direct immunofluorescence staining with CD14, CD45, IgG1, IgG2 and MPO monoclonal antibodies (McAb). Subsequent flow cytometric analysis was performed on FACSCalibur flow cytometer (Becton Dickinson, BD). As far as the antigenic expression of MPO in normal samples is concerned, FPerm and CFP demonstrated better cytoplasmic staining by inducing minor effects on light-scattering properties of the cell populations, whereas PFT-treated samples showed a diminished ability to distinguish the cell types. However, the simple and rapid FPerm method required an earlier processing of samples since the stored whole blood samples (for more than 8 hours) tended to show a significant decrease of fluorescence intensity. We also have demonstrated that P/N ratio possesses added value in evaluation of cell reactivity in immunophenotyping, based upon the apparent nonspecific cytoplasmic staining of MPO in the lymphocyte population.
    MeSH terms: Adult; Cell Separation; Cytoplasm/enzymology*; Flow Cytometry; Humans; Methods; Peroxidase/metabolism*; Permeability
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