Browse publications by year: 2000

  1. Lee WS, Davidson GP, Moore DJ, Butler RN
    J Paediatr Child Health, 2000 Aug;36(4):340-2.
    PMID: 10940167
    OBJECTIVE: To assess the validity and clinical application of a hand-held breath hydrogen (H2) analyzer (BreatH2, Europa Scientific, Crewe, UK).

    METHODOLOGY: Breath samples of patients referred to the Gastroenterology Unit, Women's and Children's Hospital, North Adelaide, South Australia, for confirmation of the diagnosis of carbohydrate malabsorption were analysed with the Quintron microlyzer (Quintron Instrument Co., Milwaukee, USA) and the BreatH2 analyser, using the Quintron microlyzer as the gold standard.

    RESULTS: Twenty-nine breath H2 tests (BHT) were performed in 29 patients aged 2 months to 61 years. The sensitivity and specificity of the BreatH2 analyser in detecting a positive BHT using the Quintron microlyser as the gold standard were 0.90 and 0.95 with positive and negative predictive values of 0.90 and 0.95, respectively. There was one false positive and one false negative reading. Bland-Altman plots showed a high degree of agreement between the values obtained with two different methods.

    CONCLUSIONS: The diagnosis of carbohydrate malabsorption, using a portable breath H2 analyser (BreatH2), achieved an acceptable degree of sensitivity and specificity, enabling it to be used where no alternative is available.

    MeSH terms: Adolescent; Adult; Breath Tests/methods; Carbohydrate Metabolism, Inborn Errors/diagnosis*; Child; Child, Preschool; Female; Humans; Hydrogen/analysis*; Infant; Male; Middle Aged; Sensitivity and Specificity; Reproducibility of Results
  2. Boo NY, Chandran V, Zulfiqar MA, Zamratol SM, Nyein MK, Haliza MS, et al.
    J Paediatr Child Health, 2000 Aug;36(4):363-9.
    PMID: 10940172
    OBJECTIVES: To identify the types of early cranial ultrasound changes that were significant predictors of adverse outcome during the first year of life in asphyxiated term infants.

    METHODOLOGY: This was a prospective cohort study. Shortly after birth, cranial ultrasonography was carried out via the anterior fontanelles of 70 normal control infants and 104 asphyxiated infants with a history of fetal distress and Apgar scores of less than 6 at 1 and 5 min of life, or requiring endotracheal intubation and manual intermittent positive pressure ventilation for at least 5 min after birth. Neurodevelopmental assessment was carried out on the survivors at 1 year of age.

    RESULTS: Abnormal cranial ultrasound changes were detected in a significantly higher proportion (79.8%, or n = 83) of asphyxiated infants than controls (39.5%, or n = 30) (P < 0.0001). However, logistic regression analysis showed that only three factors were significantly associated with adverse outcome at 1 year of life among the asphyxiated infants. These were: (i) decreasing birthweight (for every additional gram of increase in birthweight, adjusted odds ratio (OR) = 0.999, 95% confidence interval (CI) 0.998, 1.000; P = 0.047); (ii) a history of receiving ventilatory support during the neonatal period (adjusted OR = 8.3; 95%CI 2.4, 28.9; P = 0.0009); and (iii) hypoxic-ischaemic encephalopathy stage 2 or 3 (adjusted OR = 5.8; 95%CI 1.8, 18.6; P = 0.003). None of the early cranial ultrasound changes was a significant predictor.

    CONCLUSIONS: Early cranial ultrasound findings, although common in asphyxiated infants, were not significant predictors of adverse outcome during the first year of life in asphyxiated term infants.

    MeSH terms: Apgar Score; Asphyxia Neonatorum/mortality*; Asphyxia Neonatorum/physiopathology*; Brain/physiopathology*; Echoencephalography*; Female; Fetal Distress/diagnosis; Humans; Infant, Newborn; Male; Prospective Studies; Time Factors; Cohort Studies; Prevalence; Survival Rate
  3. Chua KB, Lam SK, AbuBakar S, Lim ST, Paranjothy M, Koh MT, et al.
    J Clin Virol, 2000 Aug;17(2):83-90.
    PMID: 10942088
    BACKGROUND: The clinical sign of uvulo-palatoglossal junctional (UPJ) ulcers was first noted in 1983 in a 5.5-month-old baby with exanthem subitum (ES). An earlier prospective clinical study showed that there was a strong association of UPJ ulcers and occurrence of ES with a positive predictive value of 95.3% and negative predictive value of 100%.

    OBJECTIVE: To determine the value of uvulo-palatoglossal junctional (UPJ) ulcers as an early clinical sign of exanthem subitum (ES) due to human herpesvirus 6 (HHV 6) infection.

    STUDY DESIGN: A case-control study of 20 febrile children with UPJ ulcers versus 26 febrile children without UPJ ulcers. These children were followed up for any development of ES and investigated for human herpesvirus 6 (HHV 6) as the causative agents of the febrile episodes.

    RESULTS: In this study, 20 out of 46 febrile children aged 3 months to 3 years with UPJ ulcers were virologically and/or serologically confirmed to be due to primary HHV 6 infection. The rest of the 26 children without ulcers did not have HHV 6 infection. Of the 20 children with UPJ ulcers, only 17 of the 19 children with adequate follow-up till subsidence of fever developed ES. None of the 26 children without UPJ ulcers developed ES.

    CONCLUSION: Statistically, there was a significant association of UPJ ulcers as an early sign of ES with a positive predictive value of 89.5% and negative predictive value of 100%. This finding also suggests that the presence of UPJ ulcers is a useful pathognomic clinical sign of symptomatic primary HHV 6 infection.

    MeSH terms: Antibodies, Viral/blood; Child, Preschool; DNA, Viral/genetics; Exanthema Subitum/diagnosis*; Exanthema Subitum/immunology; Exanthema Subitum/virology; Female; Humans; Infant; Leukocytes, Mononuclear/virology; Male; Palate*; Predictive Value of Tests; Serologic Tests; Tongue Diseases/virology*; Uvula*; Herpesvirus 6, Human/genetics; Herpesvirus 6, Human/immunology; Herpesvirus 6, Human/isolation & purification*; Case-Control Studies; Polymerase Chain Reaction; Oral Ulcer/virology*
  4. Shanmuga Sundara Raj S, Yamin BM, Boshaala AM, Tarafder MT, Crouse KA, Fun HK
    Acta Crystallogr C, 2000 Aug;56 (Pt 8):1011-2.
    PMID: 10944308
    In the crystal structure of the title compound, C(14)H(12)N(2)O(2), the molecule lies about a twofold axis; two carbonyl groups and the H atoms of the N-N bond are in a trans orientation with respect to each other. In the crystal, each molecule is linked to the other and vice versa by intermolecular N-H.O hydrogen bonds between the amide hydrogen and the O atoms of neighbouring molecules to form two ten-membered rings, each of which has the graph-set motif C4R(2)(2)(10). This extends as a polymeric chain along the c axis.
    MeSH terms: Amides; Hydrogen; Hydrogen Bonding; Polymers; Orientation, Spatial
  5. Shanmuga Sundara Raj S, Fun HK, Lu ZL, Xiao W, Gong XY, Gen CM
    Acta Crystallogr C, 2000 Aug;56 (Pt 8):1013-4.
    PMID: 10944309
    The crystal structure of the title compound, C(15)H(14)N(2)O(2). H(2)O, is in the keto tautomeric form and the configuration at the azomethine C=N double bond is E. The molecule is non-planar, with a dihedral angle of 27.3 (1) degrees between the aromatic rings. The crystal structure is stabilized by extensive hydrogen bonding involving the water molecule and hydrazone moiety.
    MeSH terms: Azo Compounds; Hydrazones; Hydrogen Bonding; Names; Thiosemicarbazones; Water
  6. Shanmuga Sundara Raj S, Fun HK, Lu ZL, Xiao W, Gong XY, Gen CM
    Acta Crystallogr C, 2000 Aug;56 (Pt 8):1015-6.
    PMID: 10944310
    The whole molecule of the title compound, C(19)H(14)N(4)O(2), is essentially planar, with a highly conjugated pi system. In the crystal, the molecules are packed as chains along the [011] direction connected by O-H.N intermolecular hydrogen bonds.
    MeSH terms: Hydrogen; Hydrogen Bonding; Names; Molecular Structure
  7. Jänisch T, Junghanss T
    Med. Klin. (Munich), 2000 Jul 15;95(7):392-9.
    PMID: 10943100
    Viruses have become more mobile alongside with increasing human mobility and speed of travel. At the same time we get access to information on viral outbreaks and epidemics from large parts of the world faster than ever before. Two recent epidemics will be presented to explore the value and the consequences of communicating epidemiological information through the Internet. The epidemiology, clinical features, diagnostic procedures and prophylaxis of imported viral infections are presented. Risk factors for the emergence and resurgence of viral diseases are being discussed.
    MeSH terms: Animals; Communicable Disease Control/methods*; Disease Outbreaks/prevention & control*; Disease Outbreaks/statistics & numerical data; Disease Reservoirs; Disease Vectors; Germany/epidemiology; Humans; Malaysia/epidemiology; New York City/epidemiology; Prognosis; Travel*; Virus Diseases/diagnosis; Virus Diseases/epidemiology*; Virus Diseases/transmission; Virus Diseases/virology*; Zoonoses/epidemiology; Internet
  8. Ng SW, Shanmuga Sundara Raj S, Fun HK, Razak IA, Hook JM
    Acta Crystallogr C, 2000 Aug;56 ( Pt 8):966-8.
    PMID: 10944291
    catena-Poly[dicyclohexylammonium [tributyltin-mu-(4-oxo-4H-pyran-2,6-dicarboxylato-O(2):O( 6))]], (C(12)H(24)N)[Sn(C(7)H(2)O(6))(C(4)H(9))(3)], consists of 4-oxo-4H-pyran-2,6-dicarboxylato groups that axially link adjacent tributyltin units into a linear polyanionic chain. The ammonium counter-ions surround the chain, and each cation forms a pair of hydrogen bonds to the double-bond carbonyl O atoms of the same dianionic group. The chain propagates in a zigzag manner along the c axis of the monoclinic cell. In catena-poly[methyl(phenyl)ammonium [tributyltin-mu-(pyridine-2,6-dicarboxylato-O(2):O(6))]], (C(7)H(10)N)[Sn(C(7)H(3)NO(4))(C(4)H(9))(3)], the pyridine-2, 6-dicarboxylato groups also link the tributyltin groups into a chain, but the hydrogen-bonded chain propagates linearly on the ac face of the monoclinic cell.
    MeSH terms: Models, Molecular; Molecular Conformation; Magnetic Resonance Spectroscopy; Organotin Compounds/chemistry*; Polyesters/chemistry*; Crystallography, X-Ray
  9. Lee WS, Puthucheary SD, Parasakthi N
    Ann Trop Paediatr, 2000 Jun;20(2):125-9.
    PMID: 10945063
    Extra-intestinal non-typhoidal Salmonella (NTS) infections are uncommon in developed countries but common in developing ones. The risk factors, clinical features and outcome of children admitted to the Department of Paediatrics, University of Malaya Medical Center, Kuala Lumpur from 1978 to 1998 with extra-intestinal NTS infections were reviewed. All positive cultures of NTS, blood, cerebrospinal fluid, urine, synovial, pericardial and other body secretions (except stools), were included. Of the 98 cases reviewed, 56 were boys and 42 girls. The mean age was 2.1 years (range: newborn to 14 years). Twenty-seven children were severely immunocompromised and 21 had underlying chronic medical disorders. Bacteraemia was the most commonly detected type of infection and meningitis the commonest focal infection. The overall mortality rate was 15%. An immunocompromised state or underlying chronic medical disorder was associated with increased mortality. The three serotypes most commonly isolated were S. enteritidis, S. paratyphi B and S. typhimurium. Most isolates were sensitive to antibiotics commonly used in salmonellosis.
    MeSH terms: Adolescent; Aminoglycosides/therapeutic use; Anti-Bacterial Agents/therapeutic use; Cephalosporins/therapeutic use; Child; Child, Preschool; Female; Humans; Infant; Infant, Newborn; Male; Retrospective Studies; Salmonella enteritidis; Salmonella Infections/drug therapy; Salmonella Infections/microbiology*; Salmonella Infections/mortality; Salmonella paratyphi B; Salmonella typhimurium; Serotyping; Bacteremia/drug therapy; Bacteremia/microbiology*; Immunocompromised Host; Treatment Outcome; Meningitis, Bacterial/microbiology
  10. Chan YM, Agamuthu P, Mahalingam R
    J Hazard Mater, 2000 Oct 02;77(1-3):209-26.
    PMID: 10946129
    Currently, the generated brake lining waste dust, which contains asbestos as its major component, is disposed of into a secure landfill without any additional treatment. As an alternative to this, solidification/stabilization (S/S) disposal of the dust was investigated using Portland cement alone and Portland cement mixed with activated carbon (AC), as the binders. Toxicity Characteristics Leaching Procedure (TCLP) results on the solidified matrix showed that cement was able to immobilize the heavy metals, Ba, Zn, Cr, Pb, Cu and Fe, to within the limits set by the US EPA for TCLP. Addition of AC to the cement reduced the leaching of heavy metals by an additional 4-24% compared to cement alone. The pH of the TCLP leachate extracted from virgin cement, and from dust treated with cement with or without AC was found to increase to 10.9-12.5 as opposed to an initial value of 4.93 for the TCLP extract for the untreated dust. Results of ANS 16.1 (modified) leach protocol revealed that Ba in cement-treated samples showed the highest leach rate, followed by Zn, Pb, Cr, Cu and Fe. The leach rate of heavy metals decreased with progress in time. Cement mixed with AC exhibited similar leach characteristics, however, the leach rate was lower. The linear relationship between the cumulative fraction leached (CFL) and the square root of leaching time in all cement-based samples indicate that a diffusional process is the controlling transport mechanism for the leaching of the heavy metals. The obtained Leachability Indices (L(i)) of 7.6-9.1 and 8.3-9.5 for cement and cement with AC, respectively, were low but exceeded the guidance value of 6, which clearly indicates that all the heavy metals studied are retained well within solid matrices. Cement-based S/S hardening times increased from 30 to 96 h as the dust content increased from 40 to 70 wt.%. The resulting solid matrices exhibited a compressive strength ranging from 1 to 12 MPa, which was well above the specified limit of 414 kPa for such matrices. An economic analysis indicates that the disposal costs for the dust in the only available secure landfill would increase by 40.3% if one were to go for the cement S/S option. Addition of AC to the cement would escalate this by an additional 43.8%. Although the S/S of brake lining dust using cement effectively immobilized the heavy metals of concern, cost considerations may hinder the commercial adaptation of this technique for waste disposal unless new regulatory demands are implemented.
    MeSH terms: Asbestos/metabolism*; Carcinogens/metabolism*; Manufactured Materials; Refuse Disposal*; Motor Vehicles; Metals, Heavy/analysis
  11. Loh KC, Koay ES, Khaw MC, Emmanuel SC, Young WF
    J Clin Endocrinol Metab, 2000 Aug;85(8):2854-9.
    PMID: 10946893
    Recent studies using the ratio of plasma aldosterone concentration (PAC) to PRA as the screening test for primary aldosteronism in hypertensive populations suggested that the prevalence may be as high as 5-15%, with well over half of the subjects having normal serum potassium concentrations. Despite an increasing clinical awareness of this entity, many clinicians are reluctant to consider routine screening for primary aldosteronism in essential hypertensive patients because there are few community-based prevalence studies of primary aldosteronism in different populations. Furthermore, genetic and environmental differences may affect the prevalence and presentation of primary aldosteronism in distinct populations. This study was designed to determine the prevalence of primary aldosteronism in the predominantly Chinese population in Singapore. Three hundred and fifty unselected adult hypertensive patients attending two primary care clinics had random ambulatory measurements for PAC (nanograms per dL) and PRA (nanograms per mL/h). Serum urea, creatinine, and electrolyte measurements were obtained simultaneously. Subjects with renal insufficiency (serum creatinine, >140 micromol/L) and those treated with glucocorticoids or spironolactone were excluded. Screening was considered positive if the PAC: PRA ratio was more than 20 and the PAC was more than 15 ng/dL (>416 pmol/L). Primary aldosteronism was confirmed with the determination of PAC after 2 L saline administered iv over 4 h. Adrenal computed tomographic (CT) scans were performed in biochemically confirmed cases of primary aldosteronism. Further localization with adrenal vein sampling was carried out in selected patients with equivocal findings on adrenal CT scan. Sixty-three (18%) of the 350 hypertensive patients (215 women and 135 men; age range, 23-75 yr) were screened positive for primary aldosteronism. Only 13 of these 63 subjects (21%) were hypokalemic (serum potassium, <3.5 mmol/L). Confirmatory studies were carried out in 56 (89%) of the subjects with a positive PAC:PRA ratio. Using a PAC above 10 ng/dL (>277 pmol/L) after saline infusion as the diagnostic cut-off, 16 of the 56 patients had biochemically confirmed primary aldosteronism. Hypokalemia was found in 6 of the 16 patients (37.5%) with primary aldosteronism. Subtype evaluation with adrenal CT scan and adrenal vein sampling indicated that half of the patients with primary aldosteronism may have had potentially curable unilateral adrenal adenoma. Our data suggest that primary aldosteronism occurs in at least 5% of the adult Asian hypertensive population, and approximately half of these individuals may have potentially curable, unilateral, aldosterone-producing adrenal adenoma. Our findings also confirm the poor predictive value of hypokalemia in both the diagnosis and the exclusion of primary aldosteronism.
    MeSH terms: Adult; Aged; Aldosterone/blood; Ambulatory Care Facilities; Antihypertensive Agents/therapeutic use; Blood Pressure; China/ethnology; Cross-Sectional Studies; Female; Humans; Hyperaldosteronism/complications*; Hyperaldosteronism/diagnosis; Hyperaldosteronism/epidemiology*; Hypertension/blood; Hypertension/complications*; Hypertension/epidemiology; India/ethnology; Male; Middle Aged; Potassium/blood; Renin/blood; Singapore/epidemiology; Prevalence
  12. Yumoto T
    Am J Bot, 2000 Aug;87(8):1181-8.
    PMID: 10948003
    Pollination ecology of three Durio species, D. grandiflorus, D. oblongus, and D. kutejensis (Bombacaceae), was studied in a lowland dipterocarp forest in Sarawak, Malaysia, during a peak flowering period when at least 305 species of plants bloomed in 1996. Durio has been reported to be pollinated by bats in Peninsular Malaysia. However, my observations of flower visitors and pollination experiments indicated that two species, D. grandiflorus and D. oblongus, were pollinated by spiderhunters (Nectariniidae) and that the other species, D. kutejensis, was pollinated by giant honey bees and bats as well as birds. Hand-pollination experiments showed that all three species were obligate outbreeders. A resource limitation in fruit production was suggested. The former two species were visited only by spiderhunters, and the bagged flowers that were opened for animal visitors only at night bore no fruit, while those that were opened only during the day bore fruits, at comparable fruiting ratios to open pollination. Durio kutejensis was observed to be visited by giant honey bees, birds, and bats at different times of day, and three series of bagged experiments that exposed the flowers to animal visitors at different times of day bore fruits at a comparable ratio to open-pollination.
    MeSH terms: Animals; Bees; Birds; Chiroptera; Fruit; Malaysia; Plants; Bombacaceae; Flowers; Pollination; Forests
  13. Edman JL, Koon TY
    Int J Soc Psychiatry, 2000;46(2):101-9.
    PMID: 10950358
    Two groups of college students in Malaysia, ethnic Malay and ethnic Chinese, completed a mental illness attribution and help seeking questionnaire, and these responses were also compared with the responses of their mothers. As expected, ethnic Malays rated religious items, such as God and prayer, higher than the Chinese. However, both groups rated the social and psychological causes higher than religious, supernatural or physical causes. Contrary to our predictions, there were no intergenerational differences among either ethnic group. Medical pluralism was demonstrated, as a variety of apparently contradictory help seeking behaviors received quite high ratings including doctor/pharmacy, prayer, herbal medicine and traditional healers.
    MeSH terms: Adult; Attitude to Health*; Mental Disorders/psychology*; China/ethnology; Cross-Cultural Comparison*; Ethnic Groups/psychology*; Female; Humans; Malaysia; Male; Medicine, Traditional; Middle Aged; Mothers/psychology; Patient Acceptance of Health Care; Students/psychology
  14. Deurenberg-Yap M, Schmidt G, van Staveren WA, Deurenberg P
    Int. J. Obes. Relat. Metab. Disord., 2000 Aug;24(8):1011-7.
    PMID: 10951540
    OBJECTIVE: To study the relationship between body fat percentage and body mass index (BMI) in three different ethnic groups in Singapore (Chinese, Malays and Indians) in order to evaluate the validity of the BMI cut-off points for obesity.
    DESIGN: Cross-sectional study.
    SUBJECTS: Two-hundred and ninety-one subjects, purposively selected to ensure adequate representation of range of age and BMI of the general adult population, with almost equal numbers from each ethnic and gender group.
    MEASUREMENTS: Body weight, body height, sitting height, wrist and femoral widths, skinfold thicknesses, total body water by deuterium oxide dilution, densitometry with Bodpod(R) and bone mineral content with Hologic(R) QDR-4500. Body fat percentage was calculated using a four-compartment model.
    RESULTS: Compared with body fat percentage (BF%) obtained using the reference method, BF% for the Singaporean Chinese, Malays and Indians were under-predicted by BMI, sex and age when an equation developed in a Caucasian population was used. The mean prediction error ranged from 2.7% to 5.6% body fat. The BMI/BF% relationship was also different among the three Singaporean groups, with Indians having the highest BF% and Chinese the lowest for the same BMI. These differences could be ascribed to differences in body build. It was also found that for the same amount of body fat as Caucasians who have a body mass index (BMI) of 30 kg/m2 (cut-off for obesity as defined by WHO), the BMI cut-off points for obesity would have to be about 27 kg/m2 for Chinese and Malays and 26 kg/m2 for Indians.
    CONCLUSIONS: The results show that the relationship between BF% and BMI is different between Singaporeans and Caucasians and also among the three ethnic groups in Singapore. If obesity is regarded as an excess of body fat and not as an excess of weight (increased BMI), the cut-off points for obesity in Singapore based on the BMI would need to be lowered. This would have immense public health implications in terms of policy related to obesity prevention and management.
    MeSH terms: Adipose Tissue*; Adolescent; Adult; Aged; Body Composition*; China/ethnology; Cross-Sectional Studies; Female; Humans; India/ethnology; Malaysia/ethnology; Male; Middle Aged; Obesity/ethnology*; Obesity/genetics; Singapore/epidemiology; Absorptiometry, Photon; Body Mass Index*; Deuterium Oxide; European Continental Ancestry Group/genetics; Asian Continental Ancestry Group/genetics
  15. Mason KO, Smith HL
    Demography, 2000 Aug;37(3):299-311.
    PMID: 10953805 DOI: 10.2307/2648043
    Using data from Pakistan, India, Malaysia, Thailand, and the Philippines, we explore how gender context influences (1) husband-wife concordance in the demand for children and (2) the impact of each spouse's fertility preferences on contraceptive use. We also explore whether the husband's pronatalism can explain the wife's unmet need for contraception. The results suggest that gender context has little net effect on couples' concordance, but influences the relative weight of husbands' and wives' preferences in determining contraceptive use. Analysis of women's unmet need for contraception suggests that the husbands' pronatalism contributes to wives' unmet need, but only to a relatively small degree, especially in settings where unmet need is high. This is the case because the proportion of couples with differing fertility goals is small in most communities.
    MeSH terms: Asia; Birth Rate; Contraception/utilization*; Cross-Cultural Comparison; Decision Making; Developing Countries; Family Planning Services/utilization*; Female; Freedom; Gender Identity*; Goals; Humans; India; Malaysia; Male; Pakistan; Philippines; Thailand; Spouses/psychology*; Needs Assessment
  16. Tan PH, Lui WO, Ong P, Lau LC, Tao M, Chong Y
    Cancer Genet. Cytogenet., 2000 Aug;121(1):61-6.
    PMID: 10958943
    Tumor cytogenetic analysis from 27 patients with breast cancer diagnosed at the Singapore General Hospital revealed complex karyotypic aberrations in 12 cases. The study group comprised 25 women and 2 men, ranging in age from 33 to 78 years (median 52 years). Ethnic distribution consisted of 22 Chinese, 3 Malaysian, and 2 Indian patients. Pathologic assessment disclosed 24 invasive ductal, 2 invasive mucinous, and 1 mixed invasive mucinous and ductal carcinomas. Histologic grading showed 3 grade 1, 10 grade 2, and 12 grade 3 tumors; 2 cancers were not graded, because they had been subjected to prior chemotherapy. Tumor sizes ranged from 1.5 to 10 cm (median 3 cm). Eleven cases were axillary node negative, whereas the remaining 16 node-positive cancers affected as many as 3 nodes in 8 cases and 4 or more nodes in another 8. Twenty cases demonstrated estrogen-receptor positivity, and 8 cases progesterone-receptor positivity. The spectrum of cytogenetic abnormalities involved chromosomes 1, 3, 6, 7, 8, 11, 16, and 17 and ranged from gains and deletions of both long and short arms, trisomy, monosomy, and other rearrangements. There was a trend toward the presence of karyotypic abnormalities in tumors of higher grade.
    MeSH terms: Adenocarcinoma/genetics; Adenocarcinoma/pathology; Adult; Aged; Breast Neoplasms/genetics*; Breast Neoplasms/pathology*; Adenocarcinoma, Mucinous/genetics; Adenocarcinoma, Mucinous/pathology; Chromosome Aberrations; Female; Humans; Karyotyping; Lymphatic Metastasis; Male; Middle Aged; Neoplasm Invasiveness; Carcinoma, Ductal, Breast/genetics; Carcinoma, Ductal, Breast/pathology; Breast Neoplasms, Male/genetics*; Breast Neoplasms, Male/pathology*
  17. Ismail BS, Quirinus L
    Bull Environ Contam Toxicol, 2000 Oct;65(4):530-6.
    PMID: 10960146
    MeSH terms: Acetamides/analysis*; Biodegradation, Environmental; Biological Assay; Chemistry, Physical; Half-Life; Herbicides/analysis*; Malaysia; Soil Pollutants/analysis*; Cucumis sativus; Chemical Phenomena
  18. Bidawid S, Farber JM, Sattar SA
    J Virol Methods, 2000 Aug;88(2):175-85.
    PMID: 10960705
    Immunomagnetic beads-PCR (IM-PCR), positively-charged virosorb filters (F), or a combination of both methods (F-IM-PCR) were used to capture, concentrate and rapidly detect hepatitis A virus (HAV) in samples of lettuce and strawberries experimentally contaminated. Direct reverse transcriptase-polymerase chain reaction (RT-PCR) amplification of the collected HAV-beads complex showed a detection limit of 0.5 plaque forming units (PFU) of the virus present in 1-ml of wash solution from the produce, which was several hundred-fold more sensitive than that demonstrated by RT-PCR. In separate trials, virus-containing wash solutions from the produce were passed through the filters and the captured virus was eluted with 10 ml volumes of 1% beef extract. Of the 62% filter-captured HAV, an average of 34.8% was eluted by the 1% beef extract. PCR amplification of 2 microl from this eluate failed to produce a clear positive band signal. As little as 10 PFU, present on each piece of the lettuce or strawberry, was detectable by the F-IM-PCR, which was almost 20 times less sensitive than the detection limit of 0.5 PFU by the IM-PCR. However, considering the large volumes (< or =50 ml) used in the F-IM-PCR, the sensitivity of detection could be much greater than that of the IM-PCR, which was restricted to < or =20 ml volumes. These data indicate that the F-IM-PCR method provides the potential for a greater sensitivity of detection than the IM-PCR, since low levels of virus could be detected from large volumes of sample than possible by the IM-PCR method. Although positively-charged filters captured a greater amount of virus than both the IM-PCR and F-IM-PCR methods, direct PCR amplification from beef extract eluates was not successful in detecting HAV from produce.
    MeSH terms: Antibodies, Monoclonal; Cells, Cultured; Fruit/virology*; Hepatovirus/genetics; Hepatovirus/growth & development; Hepatovirus/isolation & purification*; RNA, Viral/analysis; RNA, Viral/isolation & purification; Sensitivity and Specificity; Immunomagnetic Separation/methods; Lettuce/virology*; Reverse Transcriptase Polymerase Chain Reaction/methods
  19. Singh S, Chow VT, Chan KP, Ling AE, Poh CL
    J Virol Methods, 2000 Aug;88(2):193-204.
    PMID: 10960707
    A specific and sensitive method based on RT-PCR was developed to detect enterovirus 71 (EV71) from patients with hand, foot and mouth disease, myocarditis, aseptic meningitis and acute flaccid paralysis. RT-PCR primers from conserved parts of the VP1 capsid gene were designed on the basis of good correlation with sequences of EV71 strains. These primers successfully amplified 44 strains of EV71 including 34 strains isolated from Singapore in 1997 and 1998, eight strains from Malaysia isolated in 1997 and 1998, one Japanese strain and the neurovirulent strain EV71/7423/MS/87. RT-PCR of 30 strains of other enteroviruses including coxsackievirus A and B, and echoviruses failed to give any positive amplicons. Hence, RT-PCR with these primers showed 100% correlation with serotyping. Direct sequencing of the RT-PCR products of 20 EV71 strains revealed a distinct cluster with two major subgroups, thus enabling genetic typing of the viruses. The genetic heterogeneity of these strains culminated in amino acid substitutions within the VP1, VP2 and VP3 regions. The sequencing of a 2.9 kb fragment comprising the capsid region and the major part of 5' UTR of two Singapore strains revealed that they belonged to a group distinct from the prototype EV71/BrCr strain and the EV71/7423/MS/87 strain. The dendrogram generated from 341 bp fragments within the VP1 region revealed that the strains of Singapore, Malaysia and Taiwan belong to two entirely different EV71 genogroups, distinct from the three genogroups identified in another recent study.
    MeSH terms: Amino Acid Sequence; Asia; Base Sequence; Cell Line; Enterovirus/genetics; Enterovirus/isolation & purification*; Hand, Foot and Mouth Disease/virology*; HeLa Cells; Humans; Molecular Sequence Data; Phylogeny; RNA, Viral/analysis; RNA, Viral/isolation & purification; Sensitivity and Specificity; Sequence Alignment; Sequence Analysis, DNA; DNA Primers; Reverse Transcriptase Polymerase Chain Reaction; Sequence Analysis, Protein; Enterovirus B, Human/genetics; Enterovirus B, Human/isolation & purification; Capsid Proteins
  20. Norazlina M, Ima-Nirwana S, Gapor MT, Khalid BA
    Exp. Clin. Endocrinol. Diabetes, 2000;108(4):305-10.
    PMID: 10961363
    Vitamin E has been shown to affect bone metabolism. In this study we determined the effects of palm vitamin E and alpha-tocopherol on bone metabolism. Sprague-Dawley female rats fed with normal rat chow were divided into 4 groups and supplemented with either palm vitamin E 30 mg/kg rat weight, palm vitamin E 60 mg/kg rat weight or alpha-tocopherol 30 mg/kg rat weight. One group was not supplemented. Half of these rats were ovariectomised before supplementation was given for 10 months. As expected, bone mineral density of the ovariectomised rats fed on normal rat chow diet was lower compared to the intact rats. However, these changes were not seen in the supplemented group of rats. Both intact and ovariectomised rats supplemented with palm vitamin E 30 mg/kg rat weight had a lower bone calcium content in both femoral and vertebral bones whilst rats fed palm vitamin E 60 mg/kg rat weight or alpha-tocopherol 30 mg/kg rat weight were able to maintain bone calcium content. Alkaline phosphatase activity was elevated in ovariectomised rats supplemented with palm vitamin E 30 mg/kg rat weight and alpha-tocopherol 30 mg/kg rat weight compared to the intact rats. Alpha-tocopherol also reduced the activity of tartrate-resistant acid phosphatase post-ovariectomy. These findings indicate that both palm vitamin E and alpha-tocopherol maintained bone mineral density in ovariectomised rats but caused conflicting effects on bone calcium content. Further study is needed in order to determine the mechanisms involved.
    MeSH terms: Acid Phosphatase/metabolism; Alkaline Phosphatase/metabolism; Animals; Bone and Bones/enzymology; Calcium/metabolism; Female; Isoenzymes/metabolism; Ovariectomy*; Plant Oils/chemistry*; Vitamin E/isolation & purification*; Vitamin E/pharmacology*; Bone Density/drug effects*; Rats, Sprague-Dawley; Rats
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