Browse publications by year: 2015

  1. Mohd Zulkifly MF, Ghazali SE, Che Din N, Desa A, Raymond AA
    Malays J Med Sci, 2015 Sep;22(5):31-41.
    PMID: 28239266 MyJurnal
    BACKGROUND: The aim of this study was to address the ability of the recovery locus of control scale (RLOC) and post-traumatic stress symptoms (PTSS) to predict physical functioning among stroke patients. In addition, the best predictors within the subdomains of the RLOC and PTSS were also investigated.

    METHODS: A total of 147 stroke patients aged 33-85 years who had intact cognitive functioning were involved in the study. The Recovery Locus of Control Scale (RLOC), the Impact of Event Scale-Revised (IES-R), and the Barthel Index (ADL) were administered to respondents six weeks after stroke.

    RESULTS: The results showed that the RLOC and PTSS were significant predictors and were capable of predicting 31% of the physical functioning of stroke patients (adjusted R(2) = 0.31; P < 0.001). Furthermore, with respect to clinical factors, the affected lesion side contributed to predicting 7% of the physical functioning (R(2)= 0.07; P < 0.001). A hierarchical regression analysis found that the internal recovery locus of control (IRLOC) was a predictor capable of explaining 18% of the predicted physical functioning (adjusted R(2)= 0.18; P < 0.001). Meanwhile, avoidance was the most influential significant predictor among PTSS, contributing to 24% of the predicting physical functioning (adjusted R(2)= 0.24; P < 0.001).

    CONCLUSION: In conclusion, the RLOC and PTSS were capable of predicting physical functioning among stroke patients.

    MeSH terms: Activities of Daily Living; Cognition; Humans; Internal-External Control; Surveys and Questionnaires; Regression Analysis; Stress Disorders, Post-Traumatic; Stroke
  2. Chidambaram R
    Malays J Med Sci, 2015 Sep;22(5):78-84.
    PMID: 28239272 MyJurnal
    Complexity in the health status of patients with kidney disease forces to seek the aid of medical devices such as the central venous catheter (CVC) that is essential in order to perform hemodialysis. Elementary information about the CVC, as required for the oral healthcare professionals, has been documented so as to serve as a medical manual. This communication is the first of its kind that conjointly delineates vital considerations, which precede dental maneuvers in patients implanted with a CVC.
    MeSH terms: Health Status; Renal Dialysis; Humans; Kidney Diseases; Central Venous Catheters
  3. Fazly Ann, Z., Muhamad Syamsul Naim, N.A., Niny Fariza, J., Wan Normaziah, W.O.B., Geethamalar, S., Iswadi, M.I.
    Trop Biomed, 2015;33(1):197-202.
    MyJurnal
    Sarcocystis infection, sarcocystosis or sarcosporidiosis is the disease caused by
    zoonotic intracellular protozoan parasite with an obligatory two-host life cycle namely
    Sarcocystis spp. The affected animals are mostly asymptomatic and the parasite is discovered
    only at slaughter. The aim of this study is to determine the status of sarcocystis infection in
    large ruminants slaughtered in four abattoirs in the state of Perak. A total of eighty-six fresh
    heart muscle and oesophagus samples were collected between February 2013 to October
    2013. The samples were examined macroscopically to look for – sarcocyst. Digestion technique
    was also used to detect the sarcocyst bradyzoites. Part of the samples were preserved in
    formalin for histological examination. Out of the 86 animals, 19 (22.0%) animals were infected
    with Sarcocystis spp. 22.5% (16 of 71) of cattle and 20.0% (3 of 15) of buffalo were diagnosed
    with sarcocystis infection. Four animals were detected positive from Ipoh abattoir, followed
    by 9, 4 and 2 animals from Taiping, Teluk Intan and Tapah abattoir respectively. It is strongly
    recommended to cook meat thoroughly to reduce the incidence of the disease in humans.
    MeSH terms: Abattoirs; Animals; Buffaloes; Cattle; Digestion; Esophagus; Formaldehyde; Humans; Meat; Myocardium; Parasites; Sarcocystis; Sarcocystosis; Incidence; Bison
  4. Goh EG, Noborio K
    Int Sch Res Notices, 2015;2015:824721.
    PMID: 27347550 DOI: 10.1155/2015/824721
    A FORTRAN code for liquid water flow in unsaturated soil under the isothermal condition was developed to simulate water infiltration into Yolo light clay. The governing equation, that is, Richards' equation, was approximated by the finite-difference method. A normalized sensitivity coefficient was used in the sensitivity analysis of Richards' equation. Normalized sensitivity coefficient was calculated using one-at-a-time (OAT) method and elementary effects (EE) method based on hydraulic functions for matric suction and hydraulic conductivity. Results from EE method provided additional insight into model input parameters, such as input parameter linearity and oscillating sign effect. Boundary volumetric water content (θ L (upper bound)) and saturated volumetric water content (θ s ) were consistently found to be the most sensitive parameters corresponding to positive and negative relations, as given by the hydraulic functions. In addition, although initial volumetric water content (θ L (initial cond)) and time-step size (Δt), respectively, possessed a great amount of sensitivity coefficient and uncertainty value, they did not exhibit significant influence on model output as demonstrated by spatial discretization size (Δz). The input multiplication of parameters sensitivity coefficient and uncertainty value was found to affect the outcome of model simulation, in which parameter with the highest value was found to be Δz.
  5. Iftkhar A, Hasan IJ, Sarfraz M, Jafri L, Ashraf MA
    West Indian Med J, 2015 12;64(5):462-467.
    PMID: 27398602 DOI: 10.7727/wimj.2016.052
    Background: The present study was designed to evaluate the nephroprotective effect of the leaves of Aloe barbadensis against toxicity induced by diclofenac sodium in albino rabbits.

    Subjects and Method: Thirty-six healthy albino rabbits were randomly divided into six groups each with six animals. Group 1 served as the untreated control, group 2 was treated only with diclofenac sodium, group 3 with the nephroprotective drug silymarin and groups 4, 5, and 6 were treated with different doses of Aloe barbadensis,ie 200 mg/kg, 400 mg/kg and 600 mg/kg, respectively after being treated with diclofenac sodium. Blood samples were collected after every five days up to fifteen days. Haematological and histopathological parameters were determined by using diagnostic kits.

    Results: Results of haematological studies showed that use of the powder of Aloe barbadensis normalized the level of different factors eg, white blood cells (WBCs), red blood cells (RBCs), platelet count, packed cell volume (PCV), mean cell volume (MCV) and haemoglobin (Hb) values. Histopathological studies showed that Aloe barbadensis ameliorated pyknotic nuclei in the renal epithelial cells and reduced oxidative stress by increasing the level of catalase and decreasing malondialdehyde (MDA) level.

    Conclusion: These results have shown that Aloe barbadensis can normalize oxidative stress and can be used as an effective nephroprotective agent against drug-induced nephrotoxicity.

  6. Khan AH, Israr M, Mateen O, Hadi MA, Aftab RA
    West Indian Med J, 2015 Dec 15.
    PMID: 27399217 DOI: 10.7727/wimj.2014.234
    Gender differences among tuberculosis (TB) patients are notable; however, studies evaluating the impact of gender on treatment outcomes have reported inconsistent results.
  7. Luo J, Wang X, Yang Y, Lan T, Ashraf MA, Mao Q
    West Indian Med J, 2015 12;64(5):540-542.
    PMID: 27399315 DOI: 10.7727/wimj.2016.059
    We report a case of a patient with AIDS and a brain abscess caused by aspergillus, who underwent neurosurgical excision of the lesion and received subsequent therapy with voriconazole. The patient suffered from intracranial hypertension and visual disorders.
  8. Sarfraz M, Ashraf Y, Sajid S, Ashraf MA
    West Indian Med J, 2015 12;64(5):487-494.
    PMID: 27399795 DOI: 10.7727/wimj.2016.060
    Background: The use of chemotherapy for the treatment of cancer began at the start of the 20(th) century in an attempt to narrow the universe of chemicals that affect the disease. Metastatic testicular cancer has always been sensitive to chemotherapy.

    Subjects and Method: A retrospective and prospective study was performed of patients who had undergone testicular cancer from 2011-2013. The overall age of the testicular cancer patients at the time of diagnosis, their marital status, stage of disease and treatment strategies, testosterone level etc were analysed using linear regression and t-test.

    Results: Most of the patients had seminoma tumour. A greater number of patients were diagnosed in the later stages of the disease. Before chemotherapy, testosterone level was normal and decreased during chemotherapy but after completion, it returned to normal level.

    Conclusion: There is an early onset of testicular cancer in the Pakistani population. There is no effect of chemotherapy on testosterone production in late survivors.

  9. Khaliq T, Sarfraz M, Ashraf MA
    West Indian Med J, 2015 Dec;64(5):527-532.
    PMID: 27399905 DOI: 10.7727/wimj.2016.176
    BACKGROUND: Diabetes mellitus is an important human disease afflicting many from various walks of life in different countries. Even though modern medicines contribute a variety of effective treatment options, they can have several unfavourable effects. The intention of this review is to organize and discuss various studies that have been previously conducted on the effectiveness of these herbal plants in diabetes.

    METHOD: By using various electronic search databases, a comprehensive English literature search was conducted. Different search terms were used by combining all the search fields in titles, abstracts and keywords.

    RESULTS: Curcuma longa,a spice, is commonly known as turmeric and belongs to the family Zingiberaceae. Piper nigrumis also a spice, commonly called black pepper, and belongs to the family Piperaceae. Phoenix dactylifera, commonly known as date fruit, belongs to the family Arecaceae. From ancient times, they have been traditionally used for the treatment of various diseases. Among various activities, regulation of hyperglycaemia is considered one of their important effects. One of the aetiological factors implicated in the development of diabetes and its complications is the damage induced by free radicals. Antioxidant properties of antidiabetic compounds would be more beneficial. Extracts of these plants have shown hypoglycaemic and hypolipidaemic effects by the involvement of several mechanisms. In the future, further studies are needed to investigate the mechanisms involved in their hypoglycaemic potential and their active constituents as synthetic analogues.

    CONCLUSIONS: This review focusses on some medicinal plants that have antidiabetic effect, thus contributing to the reduction of risk factors associated with diabetes, and related beneficial effects are compiled.

  10. Zhao L, Ashraf MA
    West Indian Med J, 2015 12;64(5):506-513.
    PMID: 27400164 DOI: 10.7727/wimj.2016.179
    Background: The main reason for biomaterial related refractory infections is biofilm formation caused by bacterial adhesion on the surface of materials. Silver-hydroxyapatite (Ag/HA) nanocomposite coating can inhibit the formation of biofilm, but its mechanism is not clear.

    Material and method: In order to clarify the mechanism, the amounts of biofilm on the Ag/HA composite coating and HA coating were determined, the release rates of silver nanoparticles in simulated body fluid (SBF) were detected by atomic absorption spectrometry, and the expression values of atlE, fbe, sap, iapB genes of Staphylococcus aureus were studied when they grew on Ag/HA composite coating and HA coating.

    Results: The amount of the biofilm on the Ag/HA composite coating was significantly less than that on the HA coating, and the bacterial adhesion was decreased. The silver nanoparticles were released continuously in SBF and the release rate decreased gradually with time. The expression values of atlE, fbe and sap were high in the initial stage of adhesion and the expression value of iapB was high in the colonies-gathering stage in the control group, but they were all significantly inhibited in the presence of Ag.

    Conclusion: These results indicated that the main antibacterial effect of Ag/HA composite coating was achieved by the release of silver nanoparticles. The addition of Ag inhibited the expression of genes related to biofilm formation, which in turn inhibited the formation of biofilms. This provided theoretical support for the clinical application of Ag/HA composite coating.

  11. Kohilavani, Zzaman, W., Abdullah, W.W.N., Tajul, A.Y.
    MyJurnal
    Since early 2000, Malaysian food-export industries have recognised the demand for food
    compliant with Islamic dietary law (halal), with primary consumer choices based on quality
    and safety. The lack of documented monitoring for health hazards and haram substances led
    to the withdrawal of certification. The HACCP-based halal quality-assurance standards were
    developed as a result, using the HACCP criteria for safety, religious dietary requirement and
    quality. Halal critical control points (HlCCP) are identified using HACCP criteria and a question
    tree on HALAL processing and storage. This approach harmonizes and unifies halal processing
    with the specific food industry via an HACCP–based halal quality-assurance system.
    MeSH terms: Certification; Food; Food-Processing Industry; Nutritional Requirements; Safety; Trees; Food Industry; Hazard Analysis and Critical Control Points
  12. Hadijah, H., Norazlanshah, H., Muhammad, I., Roowi, S.
    MyJurnal
    The interest in dietary antioxidants which are mainly found in fruits, has prompted research in
    the field of commercial high antioxidant juice for healthy purposes. Fruits also are rich with antioxidants that help in reducing of degenerative diseases such as cancer, arthritis, cardiovascular
    disease and inflammation. Based on the health claims from the natural antioxidants, a new healthy juice called Mixed Fruit Juice (MFJ) has been developed by using three combinations of local fruits (soursop, mango and kasturi lime). In order to promote the commercial use of this product, the safety evaluation is needed to be carried out. The 28-days repeated toxicity test has been conducted in female and male rats for pre-clinical safety assessment prior to human study. There was no mortality observed when varying doses of the MFJ (5, 10 and 20%) administered to all rats. Hematological analysis showed no significant differences in most parameters examined. There were no significant changes observed in the liver and kidney functions tests of all treated-rats as compared to control normal rats. Furthermore, lipid profiles and blood glucose level were also within the normal range as noted in control rats. The present data demonstrate that the supplementation of MFJ did not produce adverse effects on the body system of experimental rats. This is the first documented report on the safety assessment of
    MFJ in rats.
    MeSH terms: Fruit and Vegetable Juices; Animals; Antioxidants; Arthritis; Blood Glucose; Cardiovascular Diseases; Diet; Female; Fruit; Humans; Inflammation; Lipids; Liver; Male; Neoplasms; Oxides; Reference Values; Calcium Compounds; Toxicity Tests; Dietary Supplements; Mangifera; Annona; Rats
  13. Sharifah Azizah, T.N., Nik Shanita, S., Hasnah, H.
    MyJurnal
    The aim of this study was to determine the specific content and type of sugars in selected commercial and traditional kuih in Klang Valley. The selection of the kuih was based on the validated Food Frequency Questionnaire (FFQ) for sugar. The selected commercial kuih was doughnut coated with sugar (Big Apple) while the ten traditional kuih samples consisted of kuih bingka ubi, kuih kasui, kuih keria, kuih koci, kuih lapis, kuih lopes, kuih onde-onde, kuih sagu, kuih seri muka and kuih talam. The doughnut coated with sugar (Big Apple) was purchased from Big Apple Donuts and Coffee franchise at two different locations, while the traditional kuih were randomly bought from stalls, cafeterias and restaurants around Kuala Lumpur and Rawang. The types and amount of sugar were determined using High Performance High Chromatography (HPLC) with a refractive index (RI) detector. Results showed that doughnut coated with sugar (Big Apple) has the highest starch content (22.6±0.3 g/100g) and kuih keria contained the highest available carbohydrate (41.5±1.7 g/100g), comprising of 24.2±2.4 g/100g total sugar and 17.3±0.7 g/100g of starch. The least available carbohydrate content was found in kuih talam (20.0±0.5 g/100g), which was 50% lower than the one in kuih keria. Major individual sugars detected in all kuih samples were consisted of sucrose (60.0%), glucose (16.2%), fructose (14.0%), maltose (9.5%) and lactose (0.3%). Majority of the kuih samples (90.9%) in this study can be categorized as medium sugar while only kuih keria was categorized as high sugar. Based on the two main ingredients (sugar and flour) used in the preparation of kuih, results showed that all kuih samples can be categorized as medium sugarmedium starch. In conclusion, this study served as a guideline by locals in selecting kuih of
    different sugar levels.
    MeSH terms: Chromatography, High Pressure Liquid; Coffee; Dietary Carbohydrates; Flour; Food Additives; Fructose; Glucose; Lactose; Maltose; Surveys and Questionnaires; Refractometry; Restaurants; Starch; Sucrose; Malus
  14. Suria, M.S., Mohd Afendy, A.T, Noor Azlina, M., Zamri, I.
    MyJurnal
    The use of polyclonal antibody (IgG) has recently been applied to the detection of bacteria. We developed a lateral flow assay (LFA) strip using a specific IgG in combination with colloidal gold on a nitrocellulose membrane. A conjugate, gold-anti Escherichia coli (E. coli) O157:H7 IgG was developed in this study for the detection of E. coli O157:H7 in food. The 40 nm in size of colloidal gold nanoparticles was used to conjugate the anti-E. coli O157:H7 IgG. The optimal concentration, 12.0 µg/ml of the anti-E. coli O157:H7 IgG was determined by standard curve generated in titration method. The serially diluted of E. coli O157:H7 was detected and clearly visualized on the LFA strip as low as 106 CFU/ml (result not shown). The IgG raised in rabbit have shown specific binding capacity against E. coli O157:H7. No other genus of bacteria, including Salmonella typhimurium, Listeria monocytogenes and Campylobacter jejuni reacted to the IgG. The LFA strip could also detect E. coli O157:H7 in different food samples matrices after 18 h-enrichment and this result were in accordance with the results of the polymerase chain reaction (PCR) and colony count.
    MeSH terms: Animals; Collodion; Gold; Immunoglobulin G; Listeria monocytogenes; Rabbits; Salmonella typhimurium; Campylobacter jejuni; Polymerase Chain Reaction; Gold Colloid; Escherichia coli O157; Nanoparticles
  15. Mohd Afendy, A.T., Son, R.
    MyJurnal
    Salmonella remains to be a major foodborne pathogen for animals and humans and is the
    leading cause of foodborne infections and outbreaks in various countries. Salmonella Enteritidis
    is one of the most frequently isolated serotypes in poultry and poultry products from human
    food poisoning cases. It can cause mild to acute gastroenterititis as well as other common
    food poisoning symptoms when infection takes place in human. Nucleic acid amplification
    technologies such as Polymerase Chain Reaction (PCR) is a tool that is rapid and sensitive
    for detection of bacterial pathogen. We report the successful detection of S. Enteritidis by
    PCR in raw chicken meat artificially-contaminated with serial concentration of S. Enteritidis
    using crude DNA extracts as DNA template. PCR primers, ENT-F and ENT-R targeted on sdfI
    gene were used to amplify DNA region unique to S. Enteritidis with crude DNA extract of the
    samples, yielded product with the size of 303 bp. These primers were specific to S. Enteritidis
    when tested by in-silico simulation against genome database of targeted bacterial species and
    confirmed in PCR as amplification bands were observed with S. Typhimurium, S. Polarum and
    S. Gallinarum. The established PCR can detect as few as 9.4 X 101
    CFU/ml of inoculated S.
    Enteritidis concentration and proved that pre-enrichment effect have significant effect on PCR
    detection by increasing 1000-fold of the sensitivity limit compared to the non pre-enriched
    samples. The PCR technique indicated that it can be successfully coupled with pre-enrichment
    step to offer advantage in routine screening and surveillance of bacterial contamination in food
    samples.
    MeSH terms: Animals; Chickens; Disease Outbreaks; DNA; Foodborne Diseases; Humans; Nucleic Acids; Poultry; Poultry Products; Salmonella enteritidis; Polymerase Chain Reaction; DNA Primers; Serogroup
  16. Lew, K.F., Goh, G.L, Son, R., Rukayadi, Y.
    MyJurnal
    The effects of methanolic extract of Javanese turmeric (Curcuma xanthorrhiza Roxb.) at different level of concentrations on the inactivation of Bacillus cereus, Escherichia coli, Pseudomonas spp. and Staphylococcus aureus in oyster mushroom (Pleurotus sajor-caju) were investigated. This study was conducted principally for the achievement on the best combination between the
    susceptibility of C. xanthorrhiza extract on natural microflora and foodborne pathogenic bacteria with the sensory acceptability of the soaked oyster mushroom. Three different concentrations (g/ml), 0.05%, 0.50% and 5.00%, of C. xanthorrhiza extract prepared with dilution method were designed as sanitizing agent in treating the oyster mushroom at 5 minutes and 10 minutes.
    There was significance reduction in the survival of microbial load between the untreated fresh oyster mushroom and those soaked with 0.05%, 0.50% and 5.00% rhizome extract (P
    MeSH terms: Achievement; Methanol; Bacillus cereus; Disease Susceptibility; Escherichia coli; Pseudomonas; Staphylococcal Infections; Staphylococcus aureus; Pleurotus; Rhizome; Curcuma
  17. Ismail, N.A, Noranizan, M.A., Shamsudin, R., Karim, R
    MyJurnal
    Cassava chips that exist in the current market have no standardisation and cannot be stacked
    nicely into cylindrical container. The objectives of this work are to determine the different dimension of cassava chips produced with different thickness and to develop stackable chips during mass production. Fresh cassava tubers were harvested, washed, peeled and sliced. The thickness measurements used were 1.0 mm, 1.5 mm, 1.75 mm and 2.0 mm and 1.27 mm thickness was measured from commercial potato chips as a controlled sample. Then, it was fried in deep fat fryer with the temperature of 170°C. For each thickness studied, different
    numbers of slice (10, 20, 30 and 40 slices) were fried simultaneously. Results showed that there
    are 6 shapes of fried chips produced during the frying. To conclude, thickness of the slice and
    number of slices fried simultaneously give impact towards the shape of fried chip.
    MeSH terms: Manihot; Molecular Weight; Solanum tuberosum; Temperature; Vegetables; Plant Tubers
  18. Normah, I., Nur-Hani Suryati, M.Z.
    MyJurnal
    Collagen was isolated from threadfin bream (Nemipterus japonicas) waste (mixture of scale and fin) by using 0.5 M citric acid or calamansi juice (Citrofortunella microcarpa) for 12 and 24 hrs at 4°C. The physico-chemical characteristics of the collagens were then compared with the commercial collagen. Shorter extraction time (12 hrs) and extraction using calamansi juice resulted in higher yield. The yield was 22% (12 hrs) and 20.37% (24 hrs) for collagen extracted using calamansi juice and 8.3% (12 hrs) and 6.9% (24 hrs) for collagen extracted using citric acid. Collagen extracted using calamansi juice were light yellow (L = 93.70, a = -1.84, b = 13.44) while citric acid collagens were white (L = 94.82, a = 0.31, b = 0.20). Sensory evaluation on odor recognition test showed that collagen extracted with calamansi juice has a pleasant
    natural fragrance which is sweet citrus. Electrophoresis profile indicated that the collagen were of type I comprising of α1 and α2 chains. Threadfin bream collagen contained higher amount of imino acids proline (254.72 to 275.50/1000 residues) and hydroxyproline (7.56 to 13.50/1000 residues) than commercial collagen which is 21.25 and 5.16/1000 residues, respectively. Maximum transition temperature (Tmax) falls within a close range for all the collagens ranging from 24.81 to 25.91°C. Calamansi juice collagens were more viscous compared to others. The extraction of threadfin bream collagen for 12 hrs using calamansi juice generally leads to collagen characterised by pleasant odor, reasonably high yield and more viscous. Therefore, natural source such as calamansi juice could be an alternative medium for collagen extraction.
    MeSH terms: Accidental Falls; Animals; Citrus; Collagen; Electrophoresis; Hydroxyproline; Imino Acids; Odors; Perciformes; Perfume; Proline; Taste; Citric Acid; Transition Temperature
  19. Mohd Nor Azman, A., Samsur, M., Mohammed, M., Fasihuddin, B.A.
    MyJurnal
    This study is to report the proximate compositions as well as tetrodotoxin (TTX) content in the muscles of yellow puffer fish Xenopterus naritus that collected from Kg. Manggut and Kabong, Sarawak. The internal organs of 26 and 20 specimens from Kg. Manggut and Kabong respectively were removed by the local people that had skills and experiences with the preparation of yellow puffer fish. In general, the moisture contents were ranging between 75.2% and 80.6%. X. naritus from Kabong showed higher crude protein contents (88.2% dry weight) than the same species from Kg. Manggut (87.9% dry weight) and not significantly different (p>0.05). X. naritus from Kg. Manggut demonstrated a significantly higher (p
    MeSH terms: Animals; Body Weight; Malaysia; Muscles; Tetrodotoxin; Tetraodontiformes
  20. Normah, I., Nur Anati, J.
    MyJurnal
    Threadfin bream (Nemipterus japonicas) muscle was hydrolysed using protease extracted from
    bilimbi (Averrhoa bilimbi L.) fruit. This study was performed in order to compare the efficiency of bilimbi protease in producing threadfin bream protein hydrolysate with the commercial protease; alcalase 2.4 L. Initially, protease was extracted and then purified using 40% ammonium sulfate precipitation method. The proteolytic activity of the crude extract and purified protease was determined. Precipitation using 40% ammonium sulfate resulted in bilimbi protease specific activity of 2.36 U/mg and 23.13% recovery. Threadfin bream hydrolysate was prepared based on the pH-stat method by hydrolysis for 2 hrs. Hydrolysis using bilimbi protease produced 34.76% degree of hydrolysis (DH) and 3.75% yield while hydrolysis using alcalase resulted in 86.6% DH with 22.78% yield. Alcalase hydrolysate showed higher solubility than bilimbi protease hydrolysate at pH 7 with 70.87 and 32.16% solubility, respectively. Results also showed that protein content of threadfin bream hydrolysate produced using alcalase was higher (86.86%) than those produced using bilimbi protease (22.12%). However, both hydrolysates showed low moisture content between 3.93 to 7.00%. The molecular weight distribution analysis using SDS–PAGE indicated the distribution of smaller peptides especially in alcalase hydrolysate. Overall, the results showed that alcalase is more efficient enzyme choice than bilimbi protease for preparing threadfin bream hydrolysates. However, both hydrolysates could play an important role thus contribute to the food industry.
    MeSH terms: Ammonium Sulfate; Electrophoresis, Polyacrylamide Gel; Fruit; Hydrolysis; Molecular Weight; Endopeptidases; Peptides; Protein Hydrolysates; Proteins; Solubility; Subtilisins; Food Industry; Complex Mixtures; Averrhoa
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