Browse publications by year: 2017

  1. Hans Prakash Sathasivam, Lau, Shin Hin, Noraida Khalid
    MyJurnal
    Primary intra-osseous squamous cell carcinoma (PIOSCC) is a rare tumour which occurs centrally
    within the jaws. It is believed to arise from odontogenic epithelial remnants or from pre-existing odontogenic
    cysts/tumours. A case of PIOSCC arising from an odontogenic cyst in a 57-year-old female is discussed.
    Initial clinical and radiographic examination was suggestive of an odontogenic cyst / cystic tumour. The
    lesion was enucleated and sent for diagnostic histopathology which revealed the presence of an invasive
    carcinoma arising from the walls of the odontogenic cyst. The patient then underwent right mandibular
    resection and reconstruction as well as right supra-omohyoid neck dissection. Long standing odontogenic
    cysts have the potential to undergo malignant transformation though this may not always be the case.
    Relying only on radiographic findings for the management of cyst-like lesions without obtaining
    histopathological diagnosis is extremely ill-advised.
  2. Wafa' Zahari, Ong, Wei Shen, Tan, Hong Jin, Azlina Ahmad, Khairul Bariah Ahmad Amin Noordin, Saaid Al Shehadat
    MyJurnal
    The present study aimed to determine the best polymerase chain reaction (PCR) conditions for
    amplification of odontoblast markers; alkaline phosphatase (ALP), dentin matrix protein 1 (DMP1), dentin
    sialophosphoprotein (DSPP) and osteopontin (OPN). Informed consent was obtained from the individuals
    prior to tooth extraction. RNA was extracted from odontoblasts obtained from extracted teeth using
    innuPREP RNA Mini kit (Analytik Jena, Germany). Five selected target factors in enhancing PCR: primer
    concentration, extension time, number of cycles, annealing time, and annealing temperature were
    manipulated to yield the correct size of amplicons. One step reverse transcriptase PCR reactions were
    performed using MyTaq One-Step RT-PCR kit (Bioline, USA) with a C1000 Thermal Cycler (Bio-Rad, USA)
    in a 25 µL reaction, keeping the amount of 2 ng/µL RNA, 0.25 µL reverse transcriptase, 0.5 µL RiboSafe
    Rnase inhibitor and 1X MyTaq One-Step Mix, constant. The optimal conditions were determined to be
    400nM of primers for DMP1 and DSPP, 200 nM for ALP and OPN; 30 seconds of extension time and 35
    PCR cycles for all genes; 10 seconds of annealing time for ALP, DMP1 and DSPP, 7 seconds for OPN. The
    annealing temperature were 56.4°C for ALP, 58.6°C for DMP1, 52.7°C for DSPP, and 56.3°C for OPN,
    respectively. The optimized PCR protocols produced the correct size of odontoblast markers.
  3. Ezany Yusoff, Azlina Ahmad, Suharni Mohamad, Nadia Farahana Muhammada
    MyJurnal
    Euphorbia tirucalli Linn. is traditionally used as medicine especially in the treatment of diseases
    caused by bacterial pathogens. The objectives of the present study were to identify the bioactive
    compounds in the stem of Euphorbia tirucalli Linn. using the gas chromatography-mass spectrometry (GCMS)
    analysis, and to investigate their potentials as an alternative for antimicrobial activity. Two-microliters
    of dried powdered of Euphorbia tirucalli Linn. stem were mixed with methanol followed by injection into
    splitless mode of GC-MS. Separation was achieved by Elite-5MS fused capillary column. The mass spectra
    were compared with the spectra of known components stored in the NIST and WILEY databases for
    compound identification. Forty-six chemical constituents were identified. The major constituents were
    lanosta-8,24-dien-3-ol, (3β)- (13.60%), (23S)-ethylcholest-5-en-(3β)-ol (7.02%), linoleic acid (2.96%) and
    viminalol (2.57%). Most of the active compounds present in the stem of Euphorbia tirucalli Linn. have
    previously been shown to exhibit antimicrobial properties.
  4. Prema Sukumaran, Chow, Wei Pin, Ooi, Zi Hong, Abdullah Mariam
    MyJurnal
    A study was done to assess and compare the levels of infection control practices amongst Year
    3, 4 and 5 undergraduate dental students at the Faculty of Dentistry, University of Malaya, Malaysia. This
    study also compared the levels of infection control practices between the operators and the assistants
    performing a similar task. A checklist was designed based on the Infection Control Guidelines published by
    the Faculty of Dentistry. The audit checklist criteria were specific to the operators and assistants or common
    to both. 10 pairs of students (operators and assistants) were randomly selected from Polyclinics A, B and C,
    representing Year 3, 4 and 5 students respectively (n=60). The subjects were audited as soon as they
    entered the clinic, during treatment and up until they exited the polyclinic. The data collected were analysed
    using SPSS and Rasch model. Year 3 students performed better infection control practices (1.43 logit)
    followed by Year 5 (0.96 logit) and Year 4 (0.94 logit) students. The operators in Year 5 and the assistants
    in Year 3 and 4 were more compliant to infection control practices. 100% of students complied with removal
    of gown before leaving the clinic. However, only 1.7% of dental students wore protective goggles or face
    shield during treatment and disinfected prostheses before and after inserting into patient’s mouth.
    Introduction and reinforcement of infection control practices should be incorporated at the beginning of each
    academic year for all students to allow for better practice and delivery of treatment to patients.
  5. Thavamalar Marimuthoo, Nor Atika Md Asfar, Mariati Abd. Rahman, S. Nagarajan M.P. Sockalingam
    MyJurnal
    This in-vitro study aimed to evaluate and compare the marginal leakage and penetration ability of
    a moisture-tolerant (Embrace WetBondTM) and a conventional (ClinproTM) resin-based sealants under three
    different enamel surface preparations (acid etched, acid etched and saliva contaminated and bur
    preparation and acid etched). One hundred and twenty extracted caries free human premolars teeth were
    cleaned and randomly divided into six groups of equal numbers, according to the type of sealants used and
    surface preparations. All the sealed teeth were subjected to thermocycling and immersed in a methylene
    blue dye. Each tooth was then embedded into acrylic resin before it was sectioned into four sections per
    tooth. Marginal leakage and unfilled surface area (indicating penetration depth of resin) were then
    measured using an optical 3D measurement device (Alicona Infinite Focus®). Both sealants exhibited
    comparable proportion of marginal leakage on acid etched only surfaces. Moisture-tolerant sealant showed
    the least proportion of marginal leakage on bur prepared and etched surfaces. Presence of saliva has
    detrimental effect on adhesion of both sealants. Nevertheless, depth of penetration of sealant into the
    fissures is comparable with both sealant types irrespective of the surface preparations.
  6. Mohamad Adam Bujang, Nurakmal Baharum
    MyJurnal
    Intraclass correlation coefficient (ICC) measures the extent of agreement and consistency among
    raters for two or more numerical or quantitative variables. This review paper aimed to present several tables that
    could illustrate the minimum sample sizes required for estimating the desired effect size of ICC, which is a
    measurement of the magnitude of an agreement. Determination of the minimum sample size under such
    circumstances is based on the two fundamentally important parameters, namely the actual value of the ICC and
    the number of observations made by each subject. The sample size calculations are derived from Power Analysis
    and Sample Size (PASS) software where the alpha and minimum required power is fixed at 0.05 and higher than
    0.80 respectively. A discussion on how to use these tables for determining sample sizes required for each of the
    various scenarios and the limitations associated with their use in each of these scenarios is provided.
  7. Crous PW, Wingfield MJ, Burgess TI, Hardy GESJ, Barber PA, Alvarado P, et al.
    Persoonia, 2017 Jun;38:240-384.
    PMID: 29151634 DOI: 10.3767/003158517X698941
    Novel species of fungi described in this study include those from various countries as follows: Australia: Banksiophoma australiensis (incl. Banksiophoma gen. nov.) on Banksia coccinea, Davidiellomycesaustraliensis (incl. Davidiellomyces gen. nov.) on Cyperaceae, Didymocyrtis banksiae on Banksia sessilis var. cygnorum, Disculoides calophyllae on Corymbia calophylla, Harknessia banksiae on Banksia sessilis, Harknessia banksiae-repens on Banksia repens, Harknessia banksiigena on Banksia sessilis var. cygnorum, Harknessia communis on Podocarpus sp., Harknessia platyphyllae on Eucalyptus platyphylla, Myrtacremonium eucalypti (incl. Myrtacremonium gen. nov.) on Eucalyptus globulus, Myrtapenidiella balenae on Eucalyptus sp., Myrtapenidiella eucalyptigena on Eucalyptus sp., Myrtapenidiella pleurocarpae on Eucalyptuspleurocarpa, Paraconiothyrium hakeae on Hakea sp., Paraphaeosphaeria xanthorrhoeae on Xanthorrhoea sp., Parateratosphaeria stirlingiae on Stirlingia sp., Perthomyces podocarpi (incl. Perthomyces gen. nov.) on Podocarpus sp., Readeriella ellipsoidea on Eucalyptus sp., Rosellinia australiensis on Banksia grandis, Tiarosporella corymbiae on Corymbia calophylla, Verrucoconiothyriumeucalyptigenum on Eucalyptus sp., Zasmidium commune on Xanthorrhoea sp., and Zasmidium podocarpi on Podocarpus sp. Brazil: Cyathus aurantogriseocarpus on decaying wood, Perenniporia brasiliensis on decayed wood, Perenniporia paraguyanensis on decayed wood, and Pseudocercospora leandrae-fragilis on Leandrafragilis.Chile: Phialocephala cladophialophoroides on human toe nail. Costa Rica: Psathyrella striatoannulata from soil. Czech Republic: Myotisia cremea (incl. Myotisia gen. nov.) on bat droppings. Ecuador: Humidicutis dictiocephala from soil, Hygrocybe macrosiparia from soil, Hygrocybe sangayensis from soil, and Polycephalomyces onorei on stem of Etlingera sp. France: Westerdykella centenaria from soil. Hungary: Tuber magentipunctatum from soil. India: Ganoderma mizoramense on decaying wood, Hodophilus indicus from soil, Keratinophyton turgidum in soil, and Russula arunii on Pterigota alata.Italy: Rhodocybe matesina from soil. Malaysia: Apoharknessia eucalyptorum, Harknessia malayensis, Harknessia pellitae, and Peyronellaea eucalypti on Eucalyptus pellita, Lectera capsici on Capsicum annuum, and Wallrothiella gmelinae on Gmelina arborea.Morocco: Neocordana musigena on Musa sp. New Zealand: Candida rongomai-pounamu on agaric mushroom surface, Candida vespimorsuum on cup fungus surface, Cylindrocladiella vitis on Vitis vinifera, Foliocryphia eucalyptorum on Eucalyptus sp., Ramularia vacciniicola on Vaccinium sp., and Rhodotorula ngohengohe on bird feather surface. Poland: Tolypocladium fumosum on a caterpillar case of unidentified Lepidoptera.Russia: Pholiotina longistipitata among moss. Spain: Coprinopsis pseudomarcescibilis from soil, Eremiomyces innocentii from soil, Gyroporus pseudocyanescens in humus, Inocybe parvicystis in humus, and Penicillium parvofructum from soil. Unknown origin: Paraphoma rhaphiolepidis on Rhaphiolepsis indica.USA: Acidiella americana from wall of a cooling tower, Neodactylaria obpyriformis (incl. Neodactylaria gen. nov.) from human bronchoalveolar lavage, and Saksenaea loutrophoriformis from human eye. Vietnam: Phytophthora mekongensis from Citrus grandis, and Phytophthora prodigiosa from Citrus grandis. Morphological and culture characteristics along with DNA barcodes are provided.
  8. Baker RJ, Dickins B, Wickliffe JK, Khan FAA, Gaschak S, Makova KD, et al.
    Evol Appl, 2017 09;10(8):784-791.
    PMID: 29151870 DOI: 10.1111/eva.12475
    Currently, the effects of chronic, continuous low dose environmental irradiation on the mitochondrial genome of resident small mammals are unknown. Using the bank vole (Myodes glareolus) as a model system, we tested the hypothesis that approximately 50 generations of exposure to the Chernobyl environment has significantly altered genetic diversity of the mitochondrial genome. Using deep sequencing, we compared mitochondrial genomes from 131 individuals from reference sites with radioactive contamination comparable to that present in northern Ukraine before the 26 April 1986 meltdown, to populations where substantial fallout was deposited following the nuclear accident. Population genetic variables revealed significant differences among populations from contaminated and uncontaminated localities. Therefore, we rejected the null hypothesis of no significant genetic effect from 50 generations of exposure to the environment created by the Chernobyl meltdown. Samples from contaminated localities exhibited significantly higher numbers of haplotypes and polymorphic loci, elevated genetic diversity, and a significantly higher average number of substitutions per site across mitochondrial gene regions. Observed genetic variation was dominated by synonymous mutations, which may indicate a history of purify selection against nonsynonymous or insertion/deletion mutations. These significant differences were not attributable to sample size artifacts. The observed increase in mitochondrial genomic diversity in voles from radioactive sites is consistent with the possibility that chronic, continuous irradiation resulting from the Chernobyl disaster has produced an accelerated mutation rate in this species over the last 25 years. Our results, being the first to demonstrate this phenomenon in a wild mammalian species, are important for understanding genetic consequences of exposure to low-dose radiation sources.
    MeSH terms: Silent Mutation; Animals; Arvicolinae; Disasters; DNA Mutational Analysis; Family Characteristics; Genetics, Population; Haplotypes; Humans; Radiation; Ukraine; Artifacts; Sequence Deletion; Sample Size; Radioactive Hazard Release; Genomics; Chernobyl Nuclear Accident; Genes, Mitochondrial; Genome, Mitochondrial; High-Throughput Nucleotide Sequencing; Mutation Rate
  9. Aziz SA, Clements GR, McConkey KR, Sritongchuay T, Pathil S, Abu Yazid MNH, et al.
    Ecol Evol, 2017 Nov;7(21):8670-8684.
    PMID: 29152168 DOI: 10.1002/ece3.3213
    Fruit bats provide valuable pollination services to humans through a unique coevolutionary relationship with chiropterophilous plants. However, chiropterophily in the Old World and the pollination roles of large bats, such as flying foxes (Pteropus spp., Acerodon spp., Desmalopex spp.), are still poorly understood and require further elucidation. Efforts to protect these bats have been hampered by a lack of basic quantitative information on their role as ecosystem service providers. Here, we investigate the role of the locally endangered island flying fox Pteropus hypomelanus in the pollination ecology of durian (Durio zibethinus), an economically important crop in Southeast Asia. On Tioman Island, Peninsular Malaysia, we deployed 19 stations of paired infrared camera and video traps across varying heights at four individual flowering trees in a durian orchard. We detected at least nine species of animal visitors, but only bats had mutualistic interactions with durian flowers. There was a clear vertical stratification in the feeding niches of flying foxes and nectar bats, with flying foxes feeding at greater heights in the trees. Flying foxes had a positive effect on mature fruit set and therefore serve as important pollinators for durian trees. As such, semi-wild durian trees-particularly tall ones-may be dependent on flying foxes for enhancing reproductive success. Our study is the first to quantify the role of flying foxes in durian pollination, demonstrating that these giant fruit bats may have far more important ecological, evolutionary, and economic roles than previously thought. This has important implications and can aid efforts to promote flying fox conservation, especially in Southeast Asian countries.
  10. Tan MY, Crouse KA, Ravoof TBSA, Jotani MM, Tiekink ERT
    Acta Crystallogr E Crystallogr Commun, 2017 Nov 01;73(Pt 11):1607-1611.
    PMID: 29152334 DOI: 10.1107/S2056989017014128
    The title compound, C23H21N3O2, is constructed about an almost planar disubstituted amino-urea residue (r.m.s. deviation = 0.0201 Å), which features an intra-molecular amine-N-H⋯N(imine) hydrogen bond. In the 'all-trans' chain connecting this to the terminal meth-oxy-benzene residue, the conformation about each of the imine and ethyl-ene double bonds is E. In the crystal, amide-N-H⋯O(carbon-yl) hydrogen bonds connect centrosymmetrically related mol-ecules into dimeric aggregates, which also incorporate ethyl-ene-C-H⋯O(amide) inter-actions. The dimers are linked by amine-phenyl-C-H⋯π(imine-phen-yl) and meth-oxy-benzene-C-H⋯π(amine-phen-yl) inter-actions to generate a three-dimensional network. The importance of C-H⋯π inter-actions in the mol-ecular packing is reflected in the relatively high contributions made by C⋯H/H⋯C contacts to the Hirshfeld surface, i.e. 31.6%.
  11. Tan YS, Tiekink ERT
    Acta Crystallogr E Crystallogr Commun, 2017 Nov 01;73(Pt 11):1642-1646.
    PMID: 29152341 DOI: 10.1107/S2056989017014396
    The title structures, [M(C6H12NOS2)2(C10H8N2)]·0.5C10H8N2, for M = Zn, (I), and Cd, (II), feature terminally bound 4,4'-bipyridyl ligands and non-coordinating 4,4'-bi-pyridyl mol-ecules, with the latter disposed about a centre of inversion. The coordination geometry about the metal atom is defined by two non-symmetrically chelating di-thio-carbamate ligands and a pyridyl N atom. The NS4 donor sets are distorted but, approximate to trigonal bipyramidal in each case. In the crystal, hy-droxy-O-H⋯O(hy-droxy) and hy-droxy-O-H⋯N(pyrid-yl) hydrogen bonds between the zinc-containing mol-ecules lead to a supra-molecular layer parallel to (100). The three-dimensional architecture arises as the layers are linked via methine-C-H⋯S, pyridyl-C-H⋯O(hy-droxy) and π-π [inter-centroid distance between coordinated pyridyl rings = 3.6246 (18) Å] inter-actions. Channels along the c-axis direction are occupied by the non-coordinating 4,4'-bipyridine mol-ecules, which are held in place by C-H⋯π(chelate ring) contacts.
  12. Shawkataly OB, Sirat SS, Jotani MM, Tiekink ERT
    Acta Crystallogr E Crystallogr Commun, 2017 Nov 01;73(Pt 11):1652-1657.
    PMID: 29152343 DOI: 10.1107/S2056989017014517
    In the title cluster complex hexane solvate, [Ru6(C30H32P2)(CO)22]·C6H14, two Ru3(CO)11 fragments are linked by a Ph2P(CH2)6PPh2 bridge with the P atoms equatorially disposed with respect to the Ru3 triangle in each case; the hexane solvent mol-ecule is statistically disordered. The Ru⋯Ru distances span a relatively narrow range, i.e. 2.8378 (4) to 2.8644 (4) Å. The hexyl chain within the bridge has an all-trans conformation. In the mol-ecular packing, C-H⋯O inter-actions between cluster mol-ecules, and between cluster and hexane solvent mol-ecules lead to a three-dimensional architecture. In addition, there are a large number of C≡O⋯π(arene) inter-actions in the crystal. The importance of the carbonyl groups in establishing the packing is emphasized by the contribution of 53.4% to the Hirshfeld surface by O⋯H/H⋯O contacts.
    MeSH terms: Bandages; Heterocyclic Compounds; Hexanes; Molecular Conformation; Solvents
  13. Devika BR, Girija CR, Shalini S, Jotani MM, Tiekink ERT
    Acta Crystallogr E Crystallogr Commun, 2017 Nov 01;73(Pt 11):1674-1678.
    PMID: 29152348 DOI: 10.1107/S205698901701458X
    The title zwitterion, C17H13NO2 (systematic name: 1-{(1E)-[(4-hy-droxy-phen-yl)iminium-yl]meth-yl}naphthalen-2-olate), features an intra-molecular charge-assisted N+-H⋯O- hydrogen bond. A twist in the mol-ecule is evident around the N-C(hy-droxy-benzene) bond [C-N-C-C torsion angle = 39.42 (8)°] and is reflected in the dihedral angle of 39.42 (8)° formed between the aromatic regions of the mol-ecule. In the crystal, zigzag supra-molecular chains along the a axis are formed by charge-assisted hy-droxy-O-H⋯O(phenoxide) hydrogen bonding. These are connected into a layer in the ab plane by charge-assisted hy-droxy-benzene-C-H⋯O(phenoxide) inter-actions and π-π contacts [inter-centroid distance between naphthyl-C6 rings = 3.4905 (12) Å]. Layers stack along the c axis with no specific inter-actions between them. The Hirshfeld surface analysis points to the significance C⋯H contacts between layers.
  14. Zukerman-Schpector J, Dallasta Pedroso S, Sousa Madureira L, Weber Paixão M, Ali A, Tiekink ERT
    Acta Crystallogr E Crystallogr Commun, 2017 Nov 01;73(Pt 11):1716-1720.
    PMID: 29152358 DOI: 10.1107/S2056989017014748
    The mol-ecule in the title compound, C15H12N4O2, has a twisted L-shape with the dihedral angle between the aromatic rings of the N-bound benzene and C-bound benzyl groups being 70.60 (9)°. The nitro group is co-planar with the benzene ring to which it is connected [C-C-N-O torsion angle = 0.4 (3)°]. The three-dimensional packing is stabilized by a combination of methyl-ene-C-H⋯O(nitro), methyl-ene-C-H⋯π(phen-yl), phenyl-C-H⋯π(triazol-yl) and nitro-O⋯π(nitro-benzene) inter-actions, along with weak π(triazol-yl)-π(nitrobenzene) contacts [inter-centroid distance = 3.8386 (10) Å]. The importance of the specified inter-molecular contacts has been verified by an analysis of the calculated Hirshfeld surface.
  15. Huggins JE, Guger C, Ziat M, Zander TO, Taylor D, Tangermann M, et al.
    PMID: 29152523 DOI: 10.1080/2326263X.2016.1275488
    The Sixth International Brain-Computer Interface (BCI) Meeting was held 30 May-3 June 2016 at the Asilomar Conference Grounds, Pacific Grove, California, USA. The conference included 28 workshops covering topics in BCI and brain-machine interface research. Topics included BCI for specific populations or applications, advancing BCI research through use of specific signals or technological advances, and translational and commercial issues to bring both implanted and non-invasive BCIs to market. BCI research is growing and expanding in the breadth of its applications, the depth of knowledge it can produce, and the practical benefit it can provide both for those with physical impairments and the general public. Here we provide summaries of each workshop, illustrating the breadth and depth of BCI research and highlighting important issues and calls for action to support future research and development.
    MeSH terms: California; Electroencephalography; Forecasting; Man-Machine Systems; Research; Brain-Computer Interfaces
  16. Chongmelaxme B, Sruamsiri R, Dilokthornsakul P, Dhippayom T, Kongkaew C, Saokaew S, et al.
    Complement Ther Med, 2017 Dec;35:70-77.
    PMID: 29154071 DOI: 10.1016/j.ctim.2017.09.009
    Zingiber cassumunar Roxb. known locally as "Plai" in Thai, has been used for treating bruise, sprain and musculoskeletal pain. Several pre-clinical studies demonstrated the anti-inflammatory effect of Plai. However, current evidence of clinical effects of Plai is still unclear. This study aimed to determine the clinical efficacy and safety of Plai among all identified indications. Of the 808 articles identified by a systematic review, six studies were included. Four studies were randomized controlled trials, while two studies were quasi-experimental studies involving 178 patients in intervention group and 177 patients in control group. Duration of treatment ranged from 7days to 2 months. Our findings showed that 14% Plai cream had a strong trend of benefits in pain reduction for muscle pain and ankle sprain. However, evidence supporting the effects of Plai on acne vulgaris treatment and anti-histamine effect are still unclear.
    MeSH terms: Analgesics/administration & dosage; Analgesics/pharmacology; Analgesics/therapeutic use*; Ankle; Anti-Inflammatory Agents/administration & dosage; Anti-Inflammatory Agents/pharmacology; Anti-Inflammatory Agents/therapeutic use*; Humans; Phytotherapy*; Ointments; Plant Extracts/administration & dosage; Plant Extracts/pharmacology; Plant Extracts/therapeutic use*; Sprains and Strains/drug therapy*; Thailand; Zingiberaceae*; Musculoskeletal Pain/drug therapy*
  17. Yeong SW, Choong YC
    Complement Ther Med, 2017 Dec;35:92-108.
    PMID: 29154074 DOI: 10.1016/j.ctim.2017.09.005
    OBJECTIVES: We investigated the knowledge and characteristics of herbal supplement usage of the customers of community pharmacies in a Malaysian population.

    DESIGN AND SETTING: Self-administered questionnaires (in English, Malay, or Chinese) were provided to customers at three community pharmacies in Malaysia (Ipoh, Perak). Questionnaire validation and translation validation were performed. A pilot study was conducted before actual questionnaire distribution. Informed consent was obtained from all participants.

    RESULTS: Total number of participants was 270 (99 males and 171 females) with majority from the 31-50 age group (41.5%). Among the participants, 45.6% were herbal users. The most commonly used herbal supplements were evening primrose oil (17.9%), ginkgo biloba (13.0%), and milk thistle (8.5%). The participants seemed to have sufficient knowledge regarding herbal supplements including safety, quality, and indication of use from medical literature. Participants obtained information about herbal supplements from pharmacists (26.9%), package inserts (25.2%), friends (20.5%), and the Internet (13.3%) more often than from their doctors (9.8%). Most herbal users did not inform their doctors about their usage of herbal supplements (68.3%) or the side effects (61.5%). Herbal supplement users also tended to be women, >50-year-old, and those with higher monthly household incomes.

    CONCLUSIONS: Community pharmacists have a vital role in educating their customers about the safe use of herbal supplements. The participants had sufficient knowledge about herbal supplement usage; therefore, customers of these community pharmacies may have benefitted from the advice of the pharmacists. Further studies could be carried out in future on the knowledge, skills and roles of community pharmacists in the safe use of herbal supplements.

    MeSH terms: Adolescent; Adult; Aged; Female; Humans; Health Knowledge, Attitudes, Practice*; Malaysia; Male; Phytotherapy/utilization*; Middle Aged; Patient Acceptance of Health Care; Pharmacies*; Pharmacists; Physician-Patient Relations; Plant Extracts/therapeutic use*; Surveys and Questionnaires; Residence Characteristics; Socioeconomic Factors; Dietary Supplements/utilization*; Disclosure; Information Dissemination; Young Adult; Drug-Related Side Effects and Adverse Reactions
  18. Musa NH, Mani V, Lim SM, Vidyadaran S, Abdul Majeed AB, Ramasamy K
    J Dairy Res, 2017 Nov;84(4):488-495.
    PMID: 29154736 DOI: 10.1017/S0022029917000620
    Nutritional interventions are now recommended as strategies to delay Alzheimer's disease (AD) progression. The present study evaluated the neuroprotective effect (anti-inflammation) of lactic acid bacteria (either Lactobacillus fermentum LAB9 or L. casei LABPC) fermented cow's milk (CM) against lipopolysaccharide (LPS)-activated microglial BV2 cells in vitro. The ability of CM-LAB in attenuating memory deficit in LPS-induced mice was also investigated. ICR mice were orally administered with CM-LAB for 28 d before induction of neuroinflammation by LPS. Learning and memory behaviour were assessed using the Morris Water Maze Test. Brain tissues were homogenised for measurement of acetylcholinesterase (AChE), antioxidative, lipid peroxidation (malondialdehyde (MDA)) and nitrosative stress (NO) parameters. Serum was collected for cytokine analysis. CM-LAB9 and CM-LABPC significantly (P < 0·05) decreased NO level but did not affect CD40 expression in vitro. CM-LAB attenuated LPS-induced memory deficit in mice. This was accompanied by significant (P < 0·05) increment of antioxidants (SOD, GSH, GPx) and reduction of MDA, AChE and also pro-inflammatory cytokines. Unfermented cow's milk (UCM) yielded greater cytokine lowering effect than CM-LAB. The present findings suggest that attenuation of LPS-induced neuroinflamation and memory deficit by CM-LAB could be mediated via anti-inflammation through inhibition of AChE and antioxidative activities.
    MeSH terms: Alzheimer Disease; Animals; Anti-Inflammatory Agents/administration & dosage; Antioxidants/analysis; Cattle; Cholinesterase Inhibitors/administration & dosage; Encephalitis/chemically induced; Encephalitis/prevention & control*; Female; Fermentation*; Lactobacillus/metabolism*; Lipopolysaccharides/pharmacology*; Malondialdehyde/analysis; Memory Disorders/chemically induced; Memory Disorders/prevention & control*; Mice, Inbred ICR; Milk/metabolism*; Milk/microbiology; Cytokines/analysis; Probiotics; Mice
  19. Said FA, Menon PS, Rajendran V, Shaari S, Majlis BY
    IET Nanobiotechnol, 2017 Dec;11(8):981-986.
    PMID: 29155398 DOI: 10.1049/iet-nbt.2017.0051
    In this study, the authors investigated the effects of a single layer graphene as a coating layer on top of metal thin films such as silver, gold, aluminum and copper using finite-difference time domain method. To enhance the resolution of surface plasmon resonance (SPR) sensor, it is necessary to increase the SPR reflectivity and decrease the full-width-half maximum (FWHM) of the SPR curve so that there is minimum uncertainty in the determination of the resonance dip. Numerical data was verified with analytical and experimental data where all the data were in good agreement with resonance angle differing in <10% due to noise present in components such as humidity and temperature. In further analysis, reflectivity and FWHM were compared among four types of metal with various thin film thicknesses where graphene was applied on top of the metal layers, and data was compared against pure conventional metal thin films. A 60 nm-thick Au thin film results in higher performance with reflectivity of 92.4% and FWHM of 0.88° whereas single layer graphene-on-60 nm-thick Au gave reflectivity of 91.7% and FWHM of 1.32°. However, a graphene-on-40 nm-thick Ag also gave good performance with narrower FWHM of 0.88° and reflection spectra of 89.2%.
    MeSH terms: Gold/chemistry*; Graphite/chemistry*; Silver/chemistry*; Substrate Specificity; Biosensing Techniques/instrumentation*; Surface Plasmon Resonance/instrumentation*; Uncertainty
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