Browse publications by year: 2017

  1. Majeed S, Iqbal M, Asi MR, Iqbal SZ, Selamat J
    J Food Prot, 2017 Dec;80(12):1993-1998.
    PMID: 29131682 DOI: 10.4315/0362-028X.JFP-17-117
    Recently, cultivation of high-yielding hybrid maize varieties has revolutionized maize production in Pakistan. Analyses of nutritional traits and aflatoxin (AF) contamination in these varieties can aid in the identification of susceptible and resistant varieties, particularly for cultivation in the Pakistani agro-climatic environment. Five spring maize varieties-Pioneer, Neelam, DK-919, Desi, and Hi-maize-were selected for analyses of their nutritional, tocopherol, and AF contents. Protein, carbohydrate, oil, ash, fiber, and moisture contents ranged between 8.7 and 10.8%, 68 and 71%, 3.72 and 5.56%, 1.09 and 1.81%, 1.1 and 3.1%, and 11.7 and 14.2%, respectively. Tocopherol levels in selected varieties were in the range of 461 to 1,430 μg/g. Hi-maize exhibited significantly higher protein and tocopherol contents than the other varieties, indicating its better suitability for feed and silage applications. The highest mean level of total AFs, 14.5 ± 0.12 μg/kg, was found in Desi, and results showed that the most dominant AF found in the maize varieties was AFB1. Furthermore, the results showed that the higher the level of tocopherol, the lower the concentration of total AFs and vice versa in maize varieties. The results can be used to investigate additional susceptible maize varieties that are resistant to fungal attack.
    MeSH terms: Food Contamination/analysis; Pakistan; Phenotype
  2. Ng YP, Rashid A, O'Brien F
    PLoS One, 2017;12(11):e0187861.
    PMID: 29131841 DOI: 10.1371/journal.pone.0187861
    BACKGROUND: Mental illness-related stigma is common, and is associated with poorer outcomes in people with mental illness. This study evaluated the attitudes of primary care nurses towards people with mental illness and its associated factors; and the effectiveness of a short video-based contact intervention (VBCI) in improving these attitudes using a Malay version of the 15-item Opening Minds Stigma Scale for Healthcare Providers (OMS-HC-15-M).

    METHODS: A 5-minute VBCI was developed comprising elements of psychoeducation and interviews of people with mental illness and the people they interact with, relating to experience of mental illness and recovery. A pre-post cross-sectional study was conducted on 206 randomly selected primary care nurses in Penang, Malaysia. The OMS-HC-15-M questionnaire was administered before and immediately after participants viewed the VBCI. The difference in mean pre-post VBCI scores using paired t-tests, effect size and standardised response mean (SRM) were obtained. Factors correlating to attitudes were obtained using univariate and multivariate regression analyses.

    RESULTS: Differences in pre-post VBCI score were statistically significant (p<0.001) with a 14% score reduction, a moderate effect size and SRM at 0.97 (0.85-0.11) and 1.1 (0.97-1.2) respectively. By factoring in the Minimal Detectable Change statistic of 7.76, the VBCI produced a significant improvement of attitudes in 30% of the participants. Factors associated with less stigmatising attitudes at baseline were previous psychiatry-related training, desiring psychiatric training, and positive contact with people with mental illness.

    CONCLUSIONS: This is the first study in Malaysia to show that a brief VBCI is effective in improving attitudes of primary care nurses towards people with mental illness in the immediate term. Further studies are needed to determine if these results can be sustained in the longer term and generalizable to other health care professionals. Qualitative studies are warranted to provide insight to the factors correlating to these attitudes. (300 words).

    MeSH terms: Adult; Attitude of Health Personnel*; Mental Disorders/nursing*; Female; Humans; Malaysia; Male; Middle Aged; Nurse-Patient Relations*; Nurses/psychology*; Surveys and Questionnaires; Program Evaluation; Telemedicine*; Videoconferencing; Young Adult; Social Stigma; Primary Care Nursing*
  3. Wassermann M, Raisch L, Lyons JA, Natusch DJD, Richter S, Wirth M, et al.
    PLoS One, 2017;12(11):e0187984.
    PMID: 29131856 DOI: 10.1371/journal.pone.0187984
    We examined Sarcocystis spp. in giant snakes from the Indo-Australian Archipelago and Australia using a combination of morphological (size of sporocyst) and molecular analyses. We amplified by PCR nuclear 18S rDNA from single sporocysts in order to detect mixed infections and unequivocally assign the retrieved sequences to the corresponding parasite stage. Sarcocystis infection was generally high across the study area, with 78 (68%) of 115 examined pythons being infected by one or more Sarcocystis spp. Among 18 randomly chosen, sporocyst-positive samples (11 from Southeast Asia, 7 from Northern Australia) the only Sarcocystis species detected in Southeast Asian snakes was S. singaporensis (in reticulated pythons), which was absent from all Australian samples. We distinguished three different Sarcocystis spp. in the Australian sample set; two were excreted by scrub pythons and one by the spotted python. The sequence of the latter is an undescribed species phylogenetically related to S. lacertae. Of the two Sarcocystis species found in scrub pythons, one showed an 18S rRNA gene sequence similar to S. zamani, which is described from Australia for the first time. The second sequence was identical/similar to that of S. nesbitti, a known human pathogen that was held responsible for outbreaks of disease among tourists in Malaysia. The potential presence of S. nesbitti in Australia challenges the current hypothesis of a snake-primate life cycle, and would have implications for human health in the region. Further molecular and biological characterizations are required to confirm species identity and determine whether or not the Australian isolate has the same zoonotic potential as its Malaysian counterpart. Finally, the absence of S. nesbitti in samples from reticulated pythons (which were reported to be definitive hosts), coupled with our phylogenetic analyses, suggest that alternative snake hosts may be responsible for transmitting this parasite in Malaysia.
    MeSH terms: Animals; Asia, Southeastern; Australia; Phylogeny; RNA, Ribosomal, 18S/genetics; Sarcocystis/classification; Sarcocystis/genetics; Sarcocystis/isolation & purification*; Snakes/parasitology*; DNA, Protozoan/genetics; Polymerase Chain Reaction
  4. Kim TH, Lee K, Shin SD, Ro YS, Tanaka H, Yap S, et al.
    J Emerg Med, 2017 Nov;53(5):688-696.e1.
    PMID: 29128033 DOI: 10.1016/j.jemermed.2017.08.076
    BACKGROUND: Response time interval (RTI) and scene time interval (STI) are key time variables in the out-of-hospital cardiac arrest (OHCA) cases treated and transported via emergency medical services (EMS).

    OBJECTIVE: We evaluated distribution and interactive association of RTI and STI with survival outcomes of OHCA in four Asian metropolitan cities.

    METHODS: An OHCA cohort from Pan-Asian Resuscitation Outcome Study (PAROS) conducted between January 2009 and December 2011 was analyzed. Adult EMS-treated cardiac arrests with presumed cardiac origin were included. A multivariable logistic regression model with an interaction term was used to evaluate the effect of STI according to different RTI categories on survival outcomes. Risk-adjusted predicted rates of survival outcomes were calculated and compared with observed rate.

    RESULTS: A total of 16,974 OHCA cases were analyzed after serial exclusion. Median RTI was 6.0 min (interquartile range [IQR] 5.0-8.0 min) and median STI was 12.0 min (IQR 8.0-16.1). The prolonged STI in the longest RTI group was associated with a lower rate of survival to discharge or of survival 30 days after arrest (adjusted odds ratio [aOR] 0.59; 95% confidence interval [CI] 0.42-0.81), as well as a poorer neurologic outcome (aOR 0.63; 95% CI 0.41-0.97) without an increasing chance of prehospital return of spontaneous circulation (aOR 1.12; 95% CI 0.88-1.45).

    CONCLUSIONS: Prolonged STI in OHCA with a delayed response time had a negative association with survival outcomes in four Asian metropolitan cities using the scoop-and-run EMS model. Establishing an optimal STI based on the response time could be considered.

    MeSH terms: Adult; Aged; Emergency Medical Services/standards*; Emergency Medical Services/statistics & numerical data; Female; Humans; Male; Middle Aged; Time Factors*; Cohort Studies; Logistic Models; Odds Ratio; Survival Analysis; Cardiopulmonary Resuscitation/methods; Cardiopulmonary Resuscitation/standards; Outcome Assessment (Health Care); Out-of-Hospital Cardiac Arrest/mortality*; Out-of-Hospital Cardiac Arrest/epidemiology; Out-of-Hospital Cardiac Arrest/therapy*
  5. Liew KY, Zulkiflee AB
    Braz J Otorhinolaryngol, 2017 10 19;84(6):764-771.
    PMID: 29128472 DOI: 10.1016/j.bjorl.2017.09.004
    INTRODUCTION: Nasopharyngeal carcinoma is a geographically and racially variable disease which has a high incidence in Malaysia. Based on current concepts in tumour related inflammation the inflammatory marker, neutrophil-lymphocyte ratio was tested to find its relationship with prognosis in nasopharyngeal carcinoma.

    OBJECTIVE: To investigate the effect of the neutrophil-lymphocyte ratio on prognosis in non-metastatic primary nasopharyngeal carcinoma patients and to further refine the cut off between high and low neutrophil-lymphocyte ratio values.

    METHODS: The medical charts of patients with histologically confirmed nasopharyngeal carcinoma from 1st January 2005 until 31st December 2009 were reviewed retrospectively and theneutrophil-lymphocyte ratio was calculated to see if there was any association between their higher values with higher failure rates.

    RESULTS: Records of 98 patients (n=98) were retrieved and reviewed. Only neutrophil-lymphocyte ratio (p=0.004) and tumor node metastasis staging (p=0.002) were significantly different between recurrent and non-recurrent groups, with the neutrophil-lymphocyte ratio being independent of tumor node metastasis staging (p=0.007). Treatment failure was significantly higher in the high neutrophil-lymphocyte ratio group (p=0.001). Disease free survival was also significantly higher in this group (p=0.000077).

    CONCLUSION: High neutrophil-lymphocyte ratio values are associated with higher rates of recurrence and worse disease free survival in non-metastatic nasopharyngeal carcinoma patients undergoing primary curative treatment.

    MeSH terms: Adult; Aged; Female; Humans; Lymphocytes*; Malaysia/ethnology; Male; Middle Aged; Neoplasm Recurrence, Local/blood*; Neoplasm Staging; Neutrophils*; Prognosis; Retrospective Studies; Biomarkers, Tumor/blood; Survival Analysis; Lymphocyte Count; Young Adult
  6. Hossen MS, Ali MY, Jahurul MHA, Abdel-Daim MM, Gan SH, Khalil MI
    Pharmacol Rep, 2017 Dec;69(6):1194-1205.
    PMID: 29128800 DOI: 10.1016/j.pharep.2017.07.002
    Honey contains many active constituents and antioxidants such as polyphenols. Polyphenols are phytochemicals, a generic term for the several thousand plant-based molecules with antioxidant properties. Many in vitro studies in human cell cultures as well as many animal studies confirm the protective effect of polyphenols on a number of diseases such as cardiovascular diseases (CVD), diabetes, cancer, neurodegenerative diseases, pulmonary diseases, liver diseases and so on. Nevertheless, it is challenging to identify the specific biological mechanism underlying individual polyphenols and to determine how polyphenols impact human health. To date, several studies have attempted to elucidate the molecular pathway for specific polyphenols acting against particular diseases. In this review, we report on the various polyphenols present in different types of honey according to their classification, source, and specific functions and discuss several of the honey polyphenols with the most therapeutic potential to exert an effect on the various pathologies of some major diseases including CVD, diabetes, cancer, and neurodegenerative diseases.
    MeSH terms: Animals; Antioxidants/isolation & purification; Antioxidants/pharmacology*; Cardiovascular Diseases/prevention & control; Diabetes Mellitus/prevention & control; Honey*; Humans; Neoplasms/prevention & control; Neurodegenerative Diseases/prevention & control; Polyphenols/isolation & purification; Polyphenols/pharmacology*
  7. Chan SC, Mok SY, Ng DW, Goh SY
    Biol Cybern, 2017 Dec;111(5-6):459-472.
    PMID: 29128889 DOI: 10.1007/s00422-017-0740-z
    Ultra-slow cortical oscillatory activity of 1-100 mHz has been recorded in human by electroencephalography and in dissociated cultures of cortical rat neurons, but the underlying mechanisms remain to be elucidated. This study presents a computational model of ultra-slow oscillatory activity based on the interaction between neurons and astrocytes. We predict that the frequency of these oscillations closely depends on activation of astrocytes in the network, which is reflected by oscillations of their intracellular calcium concentrations with periods between tens of seconds and minutes. An increase of intracellular calcium in astrocytes triggers the release of adenosine triphosphate from these cells which may alter transmission at nearby synapses by increasing or decreasing neurotransmitter release. These results provide theoretical support for the emerging awareness of astrocytes as active players in the regulation of neural activity and identify neuron-astrocyte interactions as a potential primary mechanism for the emergence of ultra-slow cortical oscillations.
    MeSH terms: Action Potentials/physiology*; Algorithms; Animals; Cell Communication/physiology*; Cells, Cultured; Cerebral Cortex/cytology; Embryo, Mammalian; Models, Biological; Nerve Net/physiology; Neuroglia/physiology*; Neurons/physiology*; Synapses/physiology*; Time Factors; Neural Networks (Computer)*; Rats, Wistar; Rats
  8. Abu ML, Nooh HM, Oslan SN, Salleh AB
    BMC Biotechnol, 2017 Nov 10;17(1):78.
    PMID: 29126403 DOI: 10.1186/s12896-017-0397-7
    BACKGROUND: Pichia guilliermondii was found capable of expressing the recombinant thermostable lipase without methanol under the control of methanol dependent alcohol oxidase 1 promoter (AOXp 1). In this study, statistical approaches were employed for the screening and optimisation of physical conditions for T1 lipase production in P. guilliermondii.

    RESULT: The screening of six physical conditions by Plackett-Burman Design has identified pH, inoculum size and incubation time as exerting significant effects on lipase production. These three conditions were further optimised using, Box-Behnken Design of Response Surface Methodology, which predicted an optimum medium comprising pH 6, 24 h incubation time and 2% inoculum size. T1 lipase activity of 2.0 U/mL was produced with a biomass of OD600 23.0.

    CONCLUSION: The process of using RSM for optimisation yielded a 3-fold increase of T1 lipase over medium before optimisation. Therefore, this result has proven that T1 lipase can be produced at a higher yield in P. guilliermondii.

    MeSH terms: Bacterial Proteins/genetics; Bacterial Proteins/metabolism*; Bacterial Proteins/chemistry; Lipase/genetics; Lipase/metabolism*; Lipase/chemistry; Pichia/genetics*; Pichia/metabolism*; Recombinant Proteins/genetics; Recombinant Proteins/metabolism*; Recombinant Proteins/chemistry; Research Design; Reproducibility of Results; Models, Statistical
  9. Azhari Z, Ismail MD, Zuhdi ASM, Md Sari N, Zainal Abidin I, Wan Ahmad WA
    BMJ Open, 2017 Nov 09;7(11):e017794.
    PMID: 29127228 DOI: 10.1136/bmjopen-2017-017794
    OBJECTIVE: To examine the relationship between body mass index (BMI) and outcomes after percutaneous coronary intervention (PCI) in a multiethnic South East Asian population.

    SETTING: Fifteen participating cardiology centres contributed to the Malaysian National Cardiovascular Disease Database-Percutaneous Coronary Intervention (NCVD-PCI) registry.

    PARTICIPANTS: 28 742 patients from the NCVD-PCI registry who had their first PCI between January 2007 and December 2014 were included. Those without their BMI recorded or BMI <11 kg/m2 or >70 kg/m2 were excluded.

    MAIN OUTCOME MEASURES: In-hospital death, major adverse cardiovascular events (MACEs), vascular complications between different BMI groups were examined. Multivariable-adjusted HRs for 1-year mortality after PCI among the BMI groups were also calculated.

    RESULTS: The patients were divided into four groups; underweight (BMI <18.5 kg/m2), normal BMI (BMI 18.5 to <23 kg/m2), overweight (BMI 23 to <27.5 kg/m2) and obese (BMI ≥27.5 kg/m2). Comparison of their baseline characteristics showed that the obese group was younger, had lower prevalence of smoking but higher prevalence of diabetes, hypertension and dyslipidemia. There was no difference found in terms of in-hospital death, MACE and vascular complications after PCI. Multivariable Cox proportional hazard regression analysis showed that compared with normal BMI group the underweight group had a non-significant difference (HR 1.02, p=0.952), while the overweight group had significantly lower risk of 1-year mortality (HR 0.71, p=0.005). The obese group also showed lower HR but this was non-significant (HR 0.78, p=0.056).

    CONCLUSIONS: Using Asian-specific BMI cut-off points, the overweight group in our study population was independently associated with lower risk of 1-year mortality after PCI compared with the normal BMI group.

    MeSH terms: Diabetes Mellitus; Humans; Hypertension; Malaysia; Registries; Retrospective Studies; Risk Factors; Body Mass Index*; Treatment Outcome; Dyslipidemias; Percutaneous Coronary Intervention*
  10. Rietschel L, Streit F, Zhu G, McAloney K, Frank J, Couvy-Duchesne B, et al.
    Sci Rep, 2017 Nov 10;7(1):15351.
    PMID: 29127340 DOI: 10.1038/s41598-017-11852-3
    Hair cortisol concentration (HCC) is a promising measure of long-term hypothalamus-pituitary-adrenal (HPA) axis activity. Previous research has suggested an association between HCC and psychological variables, and initial studies of inter-individual variance in HCC have implicated genetic factors. However, whether HCC and psychological variables share genetic risk factors remains unclear. The aims of the present twin study were to: (i) assess the heritability of HCC; (ii) estimate the phenotypic and genetic correlation between HPA axis activity and the psychological variables perceived stress, depressive symptoms, and neuroticism; using formal genetic twin models and molecular genetic methods, i.e. polygenic risk scores (PRS). HCC was measured in 671 adolescents and young adults. These included 115 monozygotic and 183 dizygotic twin-pairs. For 432 subjects PRS scores for plasma cortisol, major depression, and neuroticism were calculated using data from large genome wide association studies. The twin model revealed a heritability for HCC of 72%. No significant phenotypic or genetic correlation was found between HCC and the three psychological variables of interest. PRS did not explain variance in HCC. The present data suggest that HCC is highly heritable. However, the data do not support a strong biological link between HCC and any of the investigated psychological variables.
    MeSH terms: Adolescent; Adult; Child; Female; Hair/metabolism*; Humans; Male; Models, Genetic*; Twins, Dizygotic; Twins, Monozygotic; Multifactorial Inheritance*
  11. Halim SZ, Zakaria ZA, Omar MH, Mohtarrudin N, Wahab IRA, Abdullah MNH
    BMC Complement Altern Med, 2017 Nov 09;17(1):488.
    PMID: 29121900 DOI: 10.1186/s12906-017-1992-9
    BACKGROUND: Melastoma malabathricum L. (family Melastomaceae; MM) and Muntingia calabura L. (family Elaeocarpaceae; MC) have been separately reported to possess gastroprotective activity. In an attempt to develop a pharmaceutical product with antiulcer potential, the synergistic gastroprotective activity of methanolic extract of a mixture of MM and MC (MMMC) at various ratios was evaluated in rat models.

    METHODS: Rats were pre-treated orally with 2% Tween 80 (vehicle), 100 mg/kg ranitidine (reference drug) or MMMC (ratios of 1:1, 1:3 and 3:1 (v/v); doses of 15, 150 or 300 mg/kg) and then subjected to the ethanol-induced gastric ulcer or pyloric ligation assays. Stomach of rats from the former assay was collected and subjected to the macroscopic and microscopic observations, and enzymatic and non-enzymatic antioxidant studies while the gastric juice content and tissue from the latter assay were subjected to the antisecretory activity study. The UHPLC analysis of MMMC was also performed.

    RESULT: MMMC, in the ratio 1:1, demonstrated the most effective (P 

    MeSH terms: Animals; Anti-Ulcer Agents/pharmacology*; Anti-Ulcer Agents/chemistry; Antioxidants/pharmacology*; Antioxidants/chemistry; Gastric Juice/chemistry; Male; Plant Extracts/pharmacology*; Plant Extracts/chemistry; Stomach/drug effects*; Stomach/pathology; Stomach/chemistry; Stomach Ulcer/pathology; Rats, Sprague-Dawley; Plant Leaves/chemistry; Melastomataceae/chemistry*; Elaeocarpaceae/chemistry*; Rats
  12. Zhang J, Ming C, Zhang W, Okechukwu PN, Morak-Młodawska B, Pluta K, et al.
    Drug Des Devel Ther, 2017;11:3045-3063.
    PMID: 29123378 DOI: 10.2147/DDDT.S144415
    The asymptomatic properties and high treatment resistance of ovarian cancer result in poor treatment outcomes and high mortality rates. Although the fundamental chemotherapy provides promising anticancer activities, it is associated with severe side effects. The derivative of phenothiazine, namely, 10H-3,6-diazaphenothiazine (PTZ), was synthesized and reported with ideal anticancer effects in a previous paper. In this study, detailed anticancer properties of PTZ was examined on A2780 ovarian cancer cells by investigating the cytotoxicity profiles, mechanism of apoptosis, and cell invasion. Research outcomes revealed PTZ-induced dose-dependent inhibition on A2780 cancer cells (IC50 =0.62 µM), with significant less cytotoxicity toward HEK293 normal kidney cells and H9C2 normal heart cells. Generation of reactive oxygen species (ROS) and polarization of mitochondrial membrane potential (ΔΨm) suggests PTZ-induced cell death through oxidative damage. The RT2 Profiler PCR Array on apoptosis pathway demonstrated PTZ-induced apoptosis via intrinsic (mitochondria-dependent) and extrinsic (cell death receptor-dependent) pathway. Inhibition of NF-κB and subsequent inhibition of (BIRC6-XIAP) complex activities reduced the invasion rate of A2780 cancer cells penetrating through the Matrigel™ Invasion Chamber. Lastly, the cell cycle analysis hypothesizes that the compound is cytostatic and significantly arrests cell proliferation at G2/M phase. Hence, the exploration of the underlying anticancer mechanism of PTZ suggested its usage as promising chemotherapeutic agent.
    MeSH terms: Antineoplastic Agents/administration & dosage; Antineoplastic Agents/pharmacology*; Antineoplastic Agents/toxicity; Dose-Response Relationship, Drug; Female; Humans; Neoplasm Invasiveness; Ovarian Neoplasms/drug therapy*; Phenothiazines/administration & dosage; Phenothiazines/pharmacology*; Phenothiazines/toxicity; NF-kappa B/metabolism; Apoptosis/drug effects*; Reactive Oxygen Species/metabolism; Inhibitory Concentration 50; Caspases/metabolism; Cell Line, Tumor; Cell Proliferation/drug effects; Inhibitor of Apoptosis Proteins/metabolism; X-Linked Inhibitor of Apoptosis Protein/metabolism; Membrane Potential, Mitochondrial/drug effects; HEK293 Cells; G2 Phase Cell Cycle Checkpoints/drug effects; M Phase Cell Cycle Checkpoints/drug effects
  13. Mat Nuri TH, Hong YH, Ming LC, Mohd Joffry S, Othman MF, Neoh CF
    Front Pharmacol, 2017;8:739.
    PMID: 29123479 DOI: 10.3389/fphar.2017.00739
    The prevalence of Alzheimer's disease (AD) has increased with the fast growing of aging population, thereby posing great challenges to provision of care for AD patients. Pharmacists play a vital role in the management of AD; this includes recognizing early symptoms of AD, providing medication counseling to AD patients and their caretakers, and identifying potential adverse drug reactions. A comprehensive understanding of the disease progression, as well as the pharmacological therapy, is essential to provide effective care to AD patients. The level of knowledge about AD among the pharmacists, however, remains unknown. Hence, this study aimed to assess the knowledge on AD among the pharmacists in public hospitals and health clinics and its correlates. A clear picture of the characteristics associated with different levels of knowledge could facilitate the targeted re-training of pharmacists. The 30-item validated Alzheimer disease knowledge scale (ADKS) tool was pilot-tested and used in this cross-sectional study. All pharmacists, from nine public hospitals and seven public health clinics in the State of Selangor, Malaysia, were invited to participate in this cross-sectional survey. The ADKS score was computed and compared across demographics characteristics. A total of 445 pharmacists responded to the survey. These pharmacists had a moderate overall score in ADKS; nevertheless, high scores were recorded in the domains of treatment management and care giving. No difference in AD knowledge was found among pharmacists worked in public hospitals and health clinics, except for the domain of care giving (p = 0.033). Ethnicity and age group were independent predictors of ADKS score in the current study. The pharmacists in the current study had moderate AD knowledge. On-going education and training programme on AD, in particular the domains other than treatment management and care giving, should be provided to the pharmacists to ensure delivery of quality care to AD patients.
    Study site: Klinik Kesihatan, Hospitals, Selangor, Malaysia
    MeSH terms: Alzheimer Disease; Ambulatory Care Facilities; Counseling; Cross-Sectional Studies; Ethnic Groups; Hospitals, Public; Humans; Malaysia; Pharmacists; Public Health; Surveys and Questionnaires; Prevalence; Caregivers; Disease Progression; Drug-Related Side Effects and Adverse Reactions; Pilots
  14. Wu YS, Ngai SC, Goh BH, Chan KG, Lee LH, Chuah LH
    Front Pharmacol, 2017;8:761.
    PMID: 29123482 DOI: 10.3389/fphar.2017.00761
    Surfactin, a cyclic lipopeptide biosurfactant produced by various strains of Bacillus genus, has been shown to induce cytotoxicity against many cancer types, such as Ehrlich ascites, breast and colon cancers, leukemia and hepatoma. Surfactin treatment can inhibit cancer progression by growth inhibition, cell cycle arrest, apoptosis, and metastasis arrest. Owing to the potent effect of surfactin on cancer cells, numerous studies have recently investigated the mechanisms that underlie its anticancer activity. The amphiphilic nature of surfactin allows its easy incorporation nano-formulations, such as polymeric nanoparticles, micelles, microemulsions, liposomes, to name a few. The use of nano-formulations offers the advantage of optimizing surfactin delivery for an improved anticancer therapy. This review focuses on the current knowledge of surfactin properties and biosynthesis; anticancer activity against different cancer models and the underlying mechanisms involved; as well as the potential application of nano-formulations for optimal surfactin delivery.
  15. Ahmad SJ, Abdul Rahim MBH, Baharum SN, Baba MS, Zin NM
    J Trop Med, 2017;2017:2189814.
    PMID: 29123551 DOI: 10.1155/2017/2189814
    Natural products continue to play an important role as a source of biologically active substances for the development of new drug. Streptomyces, Gram-positive bacteria which are widely distributed in nature, are one of the most popular sources of natural antibiotics. Recently, by using a bioassay-guided fractionation, an antimalarial compound, Gancidin-W, has been discovered from these bacteria. However, this classical method in identifying potentially novel bioactive compounds from the natural products requires considerable effort and is a time-consuming process. Metabolomics is an emerging "omics" technology in systems biology study which integrated in process of discovering drug from natural products. Metabolomics approach in finding novel therapeutics agent for malaria offers dereplication step in screening phase to shorten the process. The highly sensitive instruments, such as Liquid Chromatography-Mass Spectrophotometry (LC-MS), Gas Chromatography-Mass Spectrophotometry (GC-MS), and Nuclear Magnetic Resonance ((1)H-NMR) spectroscopy, provide a wide range of information in the identification of potentially bioactive compounds. The current paper reviews concepts of metabolomics and its application in drug discovery of malaria treatment as well as assessing the antimalarial activity from natural products. Metabolomics approach in malaria drug discovery is still new and needs to be initiated, especially for drug research in Malaysia.
    MeSH terms: Anti-Bacterial Agents; Antimalarials; Biological Assay; Biological Products; Chromatography, Liquid; Magnetic Resonance Imaging; Malaria; Malaysia; Gas Chromatography-Mass Spectrometry; Magnetic Resonance Spectroscopy; Spectrophotometry; Streptomyces; Systems Biology; Tandem Mass Spectrometry; Metabolomics; Drug Discovery
  16. Kumar Singh A, Sulugodu Ramachandra S, Arora S, Dicksit DD, Kalyan CG, Singh P
    J Oral Biol Craniofac Res, 2017 Sep-Dec;7(3):158-160.
    PMID: 29123992 DOI: 10.1016/j.jobcr.2017.08.008
    Introduction: Oral tori and exostosis are non-pathological bony protuberances seen on the alveolar surfaces of the jaw bones. These are commonly seen on the palatal surfaces of the maxilla [torus palatinus (TP)] and around the premolars in the lingual surface of the mandible [torus mandibularis (TM)]. The aim of this cross-sectional study was to determine the prevalence of tori/exostosis in the Malaysian population.

    Methodology: A total of 2666 patients were examined for the presence of tori and exostosis in the maxilla and mandible and were categorized into TP, TM, and exostosis (facial/labial). Collected data was analysed for obtaining descriptive statistics.

    Results: 882 subjects were noticed with oral tori/exostosis among the population studied with a prevalence rate of 33%. TP was seen more in females (35%), compared to males (20%), and this difference was statistically significant (p value 
    MeSH terms: Bicuspid; Bone Neoplasms; Cross-Sectional Studies; Denture Design; Dentists; Exostoses; Female; Humans; Malaysia; Male; Mandible; Maxilla; Palate; Osteochondroma; Prevalence; Prostheses and Implants
  17. Salman M, Khan AH, Syed Sulaiman SA, Khan JH, Hussain K, Shehzadi N
    Biomed Res Int, 2017;2017:4269875.
    PMID: 29124064 DOI: 10.1155/2017/4269875
    Background: Numerous medications are known to be associated with the development of lower urinary tract symptoms (LUTS). One such medication group is calcium channel blockers (CCB).

    Objective: To critically examine the literature regarding the involvement of CCB in manifestation of LUTS in humans.

    Methods: A systematic literature search was conducted on PubMed, SciELO, Scopus, and OpenGrey databases to find all potentially relevant research studies before August 2016.

    Results: Five studies met the inclusion criteria and were included in this review. Three out of five studies stated that CCB were involved in either precipitation or exacerbation of LUTS. As for the remaining two studies, one study found out that only the monotherapy of CCB was associated with increased prevalence of nocturia and voiding symptoms in young females, whereas the other study reported an inverse association of CCB with LUTS. The methodological quality of studies was considered high for four studies and low for one study.

    Conclusion: Healthcare providers should make efforts for an earlier identification of the individuals at risk of LUTS prior to the commencement of CCB therapy. Moreover, patients should be counselled to notify their healthcare provider if they notice urinary symptoms after the initiation of CCB.

    MeSH terms: Adult; Aged; Calcium Channel Blockers/pharmacology; Calcium Channel Blockers/therapeutic use*; Female; Humans; Male; Middle Aged; Quality Assurance, Health Care; Quality of Life; Lower Urinary Tract Symptoms/drug therapy*; Lower Urinary Tract Symptoms/pathology
  18. Roslan MA, Ngui R, Vythilingam I, Sulaiman WYW
    J Vector Ecol, 2017 12;42(2):298-307.
    PMID: 29125255 DOI: 10.1111/jvec.12270
    The present study compared the performance of sticky traps in order to identify the most effective and practical trap for capturing Aedes aegypti and Aedes albopictus mosquitoes. Three phases were conducted in the study, with Phase 1 evaluating the five prototypes (Models A, B, C, D, and E) of sticky trap release-and-recapture using two groups of mosquito release numbers (five and 50) that were released in each replicate. Similarly, Phase 2 compared the performance between Model E and the classical ovitrap that had been modified (sticky ovitrap), using five and 50 mosquito release numbers. Further assessment of both traps was carried out in Phase 3, in which both traps were installed in nine sampling grids. Results from Phase 1 showed that Model E was the trap that recaptured higher numbers of mosquitoes when compared to Models A, B, C, and D. Further assessment between Model E and the modified sticky ovitrap (known as Model F) found that Model F outperformed Model E in both Phases 2 and 3. Thus, Model F was selected as the most effective and practical sticky trap, which could serve as an alternative tool for monitoring and controlling dengue vectors in Malaysia.
    MeSH terms: Aedes/physiology*; Aedes/virology; Animals; Insect Vectors/virology; Malaysia; Mosquito Control/instrumentation; Mosquito Control/methods*; Population Dynamics; Ecological Parameter Monitoring/instrumentation; Ecological Parameter Monitoring/methods
  19. Norhayati MN, Azman Yacob M
    Int J Psychiatry Med, 2017 11 08;52(4-6):328-344.
    PMID: 29117756 DOI: 10.1177/0091217417738933
    There was limited knowledge on the sexual function in women who have survived severe obstetric complications. The aim of this study was to compare the level of sexual functioning among women with and without severe morbidity at six months postpartum and to identify the factors associated with sexual function scores of women. A prospective double-cohort study design was applied at two tertiary hospitals over a six-month period. Postpartum women with and without severe maternal morbidity were selected as the exposed and the non-exposed group, respectively. The study included 145 exposed and 187 non-exposed women. Those with severe maternal morbidity were significantly ( P 
    MeSH terms: Adult; Age Factors; Female; Humans; Obstetric Labor Complications/psychology*; Length of Stay; Pregnancy; Prospective Studies; Quality of Life/psychology*; Sexual Dysfunctions, Psychological/etiology; Sexual Dysfunctions, Psychological/psychology*; Postpartum Period/psychology*; Young Adult
  20. Abdullah S, Mohtar F, Abdul Shukor N, Sapuan J
    J Hand Surg Asian Pac Vol, 2017 Dec;22(4):429-434.
    PMID: 29117830 DOI: 10.1142/S0218810417500459
    BACKGROUND: Synthetic scaffold has been used for tissue approximation and reconstructing damaged and torn ligaments. This study explores the ability of tendon ingrowth into a synthetic scaffold in vitro, evaluate growth characteristics, morphology and deposition of collagen matrix into a synthetic scaffold.

    METHODS: Upper limb tendons were harvested with consent from patients with crush injuries and non-replantable amputations. These tendons (both extensor and flexor) measuring 1 cm are sutured to either side of a 0.5 cm synthetic tendon strip and cultured in growth medium. At 2, 4, 6 and 8 weeks, samples were fixed into paraffin blocks, cut and stained with haematoxylin-eosin (H&E) and Masson's trichrome.

    RESULTS: Minimal tendon ingrowth were seen in the first 2 weeks of incubation. However at 4 weeks, the cell ingrowth were seen migrating towards the junction between the tendon and the synthetic scaffold. This ingrowth continued to expand at 6 weeks and up to 8 weeks. At this point, the demarcation between human tendon and synthetic scaffold was indistinct.

    CONCLUSIONS: We conclude that tendon ingrowth composed of collagen matrix were able to proliferate into a synthetic scaffold in vitro.

    MeSH terms: Collagen/analysis; Hand; Humans; Regeneration/physiology*; Tendons/physiology*; Tissue Scaffolds*; In Vitro Techniques
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