Introduction: Colour stability of the material is an essential factor that contributes to the selection of a flexible den- ture material. Staining effects on the denture may result in unattractive appearance, the dissatisfaction of the wearer, and limited usage period of the denture. This study aims to identify the colour stability of the Valplast flexible denture material using three types of beverages. Methods: A total of 32 disc-shaped flexible resin materials (20 +/- 0.1 mm in diameter and thickness 3 +/- 0.1 mm) were prepared based on the manufacturer instructions. The samples were randomly divided into 4 groups of immersion: distilled water (control), coffee solution, tea solution and red wine. The samples were left in the specified immersion solution for up to 60 days. Colour changes were measured after 1, 3, 7, 14, 21, 30, 45 and 60 days of immersion. Results: The samples immersed in coffee solution changed from S1 to S2, whereas samples in tea solution changed from S1 to S3. Additionally, samples in red wine changed from S1 to S4, as observed using the Valplast colour tone guide. The highest reflection measured using spectroscopy was 65.146 at 385.19 nm of the control samples, whereas the lowest reflection was 1.974 at the wavelength of 385.97 nm on the samples immersed in the red wine. Conclusion: The most significant difference can be observed in the red wine group, followed by samples in tea and coffee solutions. Moreover, increasing the immersion period causes increasing staining effect of the denture.
Introduction: Thalassemia prevalence in Malaysia is highest in Sabah. This study was conducted to characterize the demographic and socioeconomic profile of transfusion-dependent β-thalassemia major patients in Sabah, to explore their parents’ background, and to identify factors associated with having more than one thalassemia major children in the family. Methods: This was a cross-sectional study conducted in Hospital Wanita dan Kanak-Kanak Sabah and Hospital Kota Belud Sabah between February 2018 and Jun 2019. A total of 108 patients attending Thalassemia Treatment Clinic at both centers were selected randomly for interview. Results: β-thalassemia major patients in this study were majority from the indigenous ethnic groups (Kadazan, Dusun, Murut, Rungus, Sungai). Parents of the thalassemia patients mostly had low education level and socioeconomic status. Thirty-seven percent of parents have more than one children with thalassemia major and a high proportion (41.7%) have intermarriages. Parents with a higher number of children in the family are associated with having more than one thalassemia major children (OR: 2.1, 95% CI: 1.5, 2.9, P-value = 1.3 x 10-5). Conclusions: A considerably high proportion of parents have more than one children with thalassemia major in this study. Knowledge and counselling are important to parents with low educational level and socioeconomic status to ensure their understanding of thalassemia risk and prevention.
Introduction: Breast cancer is globally known to be the commonest cause of cancer-related deaths among women. Screening tools which include breast-self examination (BSE), clinical breast examination and mammography are well-recognised to aid the detection of breast cancer among high risk women. Thus, our study was aimed to evaluate breast cancer and BSE awareness and practice, and to perform mammography screening among women who attend- ed community breast cancer awareness and screening programme. Methods: Data was collected cross-sectionally from 2,021 women during community services from September 2013 until December 2015. Results: Majority of the women had previously attended health education on breast cancer (56.3%) and had been taught BSE techniques (61.4%), but only 38.6% performed BSE monthly. Factors associated with regular BSE practice were older age, higher education level, being married, higher monthly income, had attended health education on breast cancer, and had been taught BSE techniques. A subset of 130 high-risk women had underwent mammography screening and major- ity had heterogenously dense and normal mammogram findings. Conclusion: These findings suggested that regular breast cancer awareness campaigns is needed to strengthen breast cancer knowledge and to emphasise BSE tech- niques. Such campaigns should target younger women and those with low socioeconomic status.
Introduction: The National Blood Center, Kuala Lumpur interprets laboratory results for the von Willebrand factor (VWF) profile based on guidelines which were established based on the Caucasian population. The VWF profiles among the Malay population has not yet been established. The current study aims to determine the VWF profiles of the different ABO blood types among Malays and to evaluate their association with demographic characteristics and smoking habits. Methods: One hundred and forty Malay donors were involved. Factor VIII (FVIII:C), VWF antigen (VWF:Ag), and ristocetin cofactor (VWF:RiCof) levels and collagen binding activity (VWF:CBA) were measured by coagulometric clot detection, latex agglutination, and enzyme-linked immunosorbent assay. Results: The majority of donors (59.3%) were 30–49 years old, male (81.43%), non-smokers (74.3%), and overweight (71.4%). The Malay VWF:Ag were slightly higher than those of Caucasians, Indians, Thais, and Chinese, but the average ratios of VWF activity (i.e., VWF:RiCof level and VWF:CBA) to VWF:Ag were slightly lower than those of the other populations. The highest level of VWF:Ag was found among those with the B blood group, followed by types A and O. Conclusion: Malays with type O blood had lower values of the components of the VWF profile compared to subjects with non-O blood. The higher levels of these elements and lower VWF activity to VWF:Ag ratio in Malays compared to other populations suggest that ethnicity impacts the plasma VWF levels and their interaction with collagen and platelets.
Introduction: Chrysanthemum rubellum (durian) flower (CR) is well-known for its usefulness in conventional and advanced medicine. Bioactive glass properties are geared towards hard tissue regeneration. Hence, this study aims to investigate the response of dental pulp stem cells (DPSC) when exposed to bioactive glass-chrysanthemum flower ex- tract-conditioned medium. Methods: Chrysanthemum rubellum (durian) yellow coloured petals freeze dried extracts (CRE) was prepared by separation and agitation in distilled water where the final powdery compound was being investigated and combined with melt-derived BG 45S5 powder to produce BGCRE-conditioned medium. The CRE extracts in various concentrations and BGCRE-conditioned medium were exposed to DPSC and the cells responses were assessed using AB and MTT assays. The CRE and BGCRE-conditioned media were also assessed using ICP-OES to check for ionic release profile from both medium. Results: The CRE-conditioned medium (7.81, 15.63, 31.25 and
62.5 μg/ml) showed a dose-dependent effect towards DPSC from Days 1 until 14. The BGCRE-conditioned medium containing BG powders (1 mg/ml) with the lower amount of CRE extracts (0.02 and 0.1 mg/ml) promoted DPSC via- bility and proliferation rate from Days 1 until 14 based on AB and MTT assays. The BGCRE-conditioned medium has potentially affected the DPSC viability and proliferation. Conclusion: The presence of CRE in BGCRE-conditioned medium enhanced the DPSC viability and proliferation possibly through the combined effect of CRE and BG. This BGCRE combination showed potential as natural medicament for dental tissue regeneration.
Introduction: Coated archwires improve aesthetics because of the tooth-coloured appearance. However, colour change of the coated archwires have been reported in vitro. Nonetheless, little is known to what extent this colour change occurs clinically. This second part of the multi-centre, double-blind, randomised controlled trial evaluated the colour change (E*) of three coated archwires with their controls and patient perception. Methods: 84 patients who received treatment with upper and lower fixed appliance treatment were invited. Consented patients were randomised to receive one of four treatment interventions using 0.014” superelastic nickel-titanium archwires from
(1) Orthocare (2) RMO (3) G&H, and (4) 3M Unitek® uncoated. These archwires were ligated during bonding and collected after 8th week. After removal, the digital images of the archwires were assessed for colour change using Adobe® Photoshop® software, and the CIE L*a*b* system was used to calculate the ΔE* values. Patient perception was measured using oral aesthetic subjective impact scale (OASIS) questionnaire. Results: For colour change and patient perception assessment, 132 archwires from 66 participants who had been treated with aesthetic archwires were collected. Two fractured archwires were excluded. Initial analysis revealed all three aesthetic archwires groups showed significant mean of E* with the highest was found in Orthocare (23.9), and the lowest is G&H (16.8). Post hoc comparison revealed statistically significant mean of E* in Orthocare when compared with other groups (p
Introduction: Goniothalamin (GTN) is a plant styryl-lactone that has been reported to possess anticancer properties. However, limited studies were conducted to explore the selectivity of GTN to human cancer cells in comparison with normal human cells counterpart. The aim of this study was to evaluate the cytotoxicity and selectivity of GTN in human cancer cells lines of osteosarcoma cells (Saos-2), adenocarcinoma alveolar basal epithelial cells (A549), breast carcinoma cells (UACC-732), breast adenocarcinoma cells (MCF-7), colorectal adenocarcinoma cells (HT29). Human bone marrow-derived mesenchymal stem cells (HMSC) served as a control normal cell. Methods: The an- tiproliferative activity of GTN was assessed by MTT assay for 24, 48 and 72 h of incubation time. Then, the half maximal inhibitory concentration (IC50) and the selectivity index (S.I) were calculated to examine the potency and selectivity of GTN. Results: GTN has significantly exhibited concentration and time-dependent inhibitory effect to all tested cancer cells with the lowest IC50 values ranging from 0.62±0.06 to 2.01±0.28 µg/ml after 72 h. Besides, GTN was found to be better than doxorubicin, DOX (positive control) in term of its selectivity towards cancer cells as higher S.I values between 2.86±0.31 to 10.02±1.49 were determined for all the cancer cells. Conclusion: This study concludes that GTN fulfills the basic criteria as an effective therapeutic agent by possessing a very strong range of anticancer activity with less toxicity towards the normal cells. Thus, GTN could be considered as a promising alternative to present chemotherapeutic drug in cancer treatment.
Introduction: Inadequate mixing during the blood collection process might affect the quality of platelet concentrates (PCs). Currently, two different mixing methods are used during whole blood collection: manual mixing and mixing using an automated blood collection mixer. However, the cost between manual and automated blood collection mixer differed largely and pose a dilemma for a blood transfusion service. The objective of this study was to evaluate PCs quality using either manual mixing or automated procedure. Methods: This was a cross-sectional study conduct- ed at the Advanced Medical and Dental Institute, Universiti Sains Malaysia. Thirty eligible participants aged 20 to 45 were included in this study, and a unit of 450 mL whole blood was collected from each participant. Fifteen units of whole blood were mixed by an automated blood collection mixer and the other 15 units were mixed using the man- ual mixing. All PCs were produced from platelet-rich plasma and stored at 20–24°C for 5 days. Quality parameters such as platelet count, leucocyte count, and pH were measured for each PCs on day 1 and day 5. Results: Platelet count on day 1 was significantly higher than on day 5 (p = 0.01) for both mixing methods. There was no statistically significant difference in any of the PCs quality parameters between the two types of mixing methods at either day 1 or day 5 of storage (p > 0.05). Conclusion: Comparable PCs quality is achieved from both manual mixing and auto- mated procedures.
Introduction: The alkaloids present in Catharanthus roseus (C. roseus), vinblastine and vincristine are important an- ticancer agents that cause cell cycle arrest and apoptosis in various types of cell lines. However, there is no previous reports that emphasized the clear mechanisms of anticancer exerted by a crude aquoeus extract of C. roseus although it has been historically used to treat various diseases. Methods: The cytotoxicity effects of C. roseus aqueous extract on Jurkat cells were evaluated by annexin/PI staining, caspase 3/7 assay, JC-1 assay and cell cycle assay. Gene ex- pression profiling was performed by using SmartChip Real-Time PCR system to evaluate the expression profiles of on- cology-related genes of Jurkat cells treated with C. roseus aqueous extract. Results: Flow cytometry analysis revealed that the extract has caused S-phase arrest and associated with apoptosis through the externalization of phosphati- dylserine and depletion of mitochondrial membrane potential in time-dependent manner. The apoptosis mechanism was mediated through the activation of caspase 3/7. From the gene expression analysis, 8 differentially regulated genes were associated with apoptosis which were CDKN1C, CHI3L2, BIRC8, GFER, ID3-1, BBC3-2, TRAF4 and VCAN. Meanwhile, 7 differentially regulated genes were associated with cell cycle progression which were PIMI-1, CDKN1C, SKP1A, CDC25C, LTBP1, CCNG2 and RBL1. Conclusion: The recent data may facilitate the identification of specific targeting pathways induced by the extract. The information obtained may be used as diagnostic tools, prognostic markers, and predictors of response to C. roseus treatment especially for this particular type of cancer.
Introduction: Arterial and venous thromboses contribute to significant morbidity and mortality rate, thus an anti- thrombotic agent is needed for prevention and treatment of thrombosis. Ajwa dates (Phoenix dactylifera L.) report- edly contain a high level of salicylic acid which is a compound responsible for anticoagulation via antagonism of vitamin K. The present study was designed to assess coagulation activities in human plasma treated with Ajwa date extracts in vitro. Methods: Platelet-poor plasma samples from 27 donors were treated with ethanol crude date extract (ET) or aqueous crude date extract (AQ) of Ajwa dates at different concentrations to generate the following seven test groups from each donor: control (normal saline), ET-I (0.1 g/mL), ET-II (0.5 g/mL), ET-III (1.0 g/mL), AQ-I (0.1 g/ mL), AQ-II (0.5 g/mL) and AQ-III (1.0 g/mL). In vitro coagulation activities of Ajwa dates were assessed based on prothrombin time (PT, an assessment of extrinsic coagulation pathway), activated partial thromboplastin time (APTT, an assessment of the intrinsic coagulation pathway), and thrombin time (TT, an evaluation of level and function of fibrinogen). Results: A very significant prolongation of PT, APTT and TT were observed for the ET-II and ET-III groups and very significant prolongation of PT and TT was observed for the AQ-II and AQ-III groups. Significant prolonga- tion of TT was observed in the AQ-I group. Conclusion: In conclusion, Ajwa date extracts had an anticoagulation effect on human plasma.
Knee osteoarthritis (OA) is a common musculoskeletal condition that leads to decreased physical performance and falls among older adults. However, there is limited information comparing physical performances among older adult fallers with and without knee OA. The aim of this study was to compare physical performance between older adult fallers with and without knee OA. Participants were divided into two groups using the clinical diagnosis of knee OA based on National Institute of Health and Care Excellence guidelines; with and without knee OA. Participants performed a battery of physical performance tests that included chair sit and reach, back scratch, dominant handgrip strength, timed up and go (TUG), 30 seconds chair stand and 2 min walk. Independent t-test was used to compare physical performance between groups. Thirteen older adult fallers with knee OA (mean age = 70.92 ± 8.83 years) and 20 older adult fallers without knee OA (76.4 ± 7.92 years) participated in this study. There were no significant differences in sociodemographic profiles and physical performance among older adult fallers with and without knee OA (p > 0.05). However, hamstring flexibility was not within norm in 64% of older adult fallers with knee OA compared to 42% without OA. Physical performance between fallers with and without clinically diagnosed knee OA was similar. Hence, physiotherapy interventions for both groups may be similar and focused on strength and endurance training. In addition, to minimize falls risk and further improve physical performance, hamstring stretching must be emphasized among older adults with knee OA.
Children with dyslexia are commonly associated with gross motor difficulties. However, this non-literacy symptom is often overlooked as an important feature of dyslexia. Therefore, the aims of this study were to determine gross motor skills status among children with dyslexia and to compare the gross motor skills between younger and older children. A cross-sectional study was conducted on children with dyslexia from government schools and Dyslexia Association Malaysia. The participants were divided into two groups, namely younger (4 to 10 years) and older children (11 to 17 years old), and were recruited randomly. The gross motor skills were measured using the Bruininks-Oseretsky Test of Motor Proficiency, Second Edition (BOT-2) (subtests: Body Coordination, and, Strength and Agility) and the Movement Assessment Battery for Children, Second Edition (MABC-2) (subtest: Aiming and Catching). Standard scores and percentile rankings were used for statistical analysis. A total of 82 children with dyslexia were recruited for this study, consisted of younger (n = 57) and older groups of children (n = 25). The younger children were found to have a significantly lower performance in gross motor skills in Body Coordination (Mdn = 48, IQR = 26-63), compared to the older children, (Mdn = 54, IQR = 30-77), with a small effect size (r = -.25). There were no significant different for other findings. The older children were found to demonstrate the highest level of gross motor skills. This study may suggest the need for an early intervention program for young age children with dyslexia. Future studies that assess gross motor skills using longitudinal design are recommended to investigate the changes in gross motor performance over time.
Despite being becoming popular and heavily used in rehabilitation, the evidence on the effectiveness of Virtual reality-based therapy (VRBT) among persons with knee osteoarthritis (OA) remains limited. The aim of this study was to determine the effects of VRBT on walking endurance and quality of life of older persons with knee OA in comparison to conventional exercise therapy. This was a randomised controlled trial involving 44 participants (mean age and standard deviation = 66.32 ± 5.73 years) who received either a VRBT (experimental group, n = 22) or a conventional exercise therapy (control group, n = 22) for 45 minutes, twice per week for 12 weeks. The outcome of both therapies on walking endurance was measured using Six-Minute Walk Test (6 MWT), while quality of life was assessed using the Malay-version of Short Form-36 (SF-36). Analysis was done by ‘intention-to-treat’ approach, using Mixed Model ANOVA. A significant time-effect (p < 0.05), with large effect size between 0.43 and 0.54 were found for all outcomes following the therapies. An increase of 17% and 19% of 6 MWT scores were obtained in experimental and control group, respectively. Meanwhile, the scores for the physical and mental components of the SF-36 increased by 37.8% and 19.4% in the experimental group, and 45.7% and 20.6% in the control group. However, no significant group and interaction effects were found for all outcomes (p > 0.05). In conclusion, VRBT is comparable with conventional exercise therapy in improving walking endurance and quality of life of persons with knee OA, and can be used as a therapy option for this population.
Older adults who fall recurrently have sub-optimal physical performance especially muscle strength, mobility and balance. Recurrent falls lead to a heightened fear of falls. However, there is limited information regarding other domains of physical performance, namely flexibility and endurance. In addition, there is still limited knowledge pertaining fear of falls and physical activity levels among Malaysian community-dwelling older adults who are recurrent fallers. The aim of our study was to compare fear of falls, physical performance and physical activity levels among older adults with falls and recurrent falls. This cross- sectional study was carried out in two Malaysian urban districts. Physical performance was examined using Functional Fitness MOT (FFMOT). Modified Baecke Questionnaire and Activities-Specific Balance Confidence (ABC-6) Scale were administered to evaluate the level of daily physical activity and fear of falls respectively. A total of 35 older adults participated in this study, 20 fallers (mean age: 71.95±8.22) and 15 recurrent fallers (mean age: 76.73±8.82) respectively. Our study results showed that older adults with recurrent falls had significantly reduced lower extremity strength, dynamic balance, endurance, balance confidence in activities of daily living and physical activity levels compared to faller group (p < 0.05). Although flexibility and upper limb strength were not significantly different among fallers and recurrent fallers, overall improvement in physical performance should be targetted in falls prevention and management. This is vital to prevent further deterioration in physical performance among older adults with recurrent falls.
The development of writing ability is not only important in building a child’s self-esteem, but also essential for academic success in school. Handwriting is an important skill that could affect students’ performance in most academic areas. This study aimed to review the effect of occupational therapy intervention in improving handwriting skills among preschool children. Relevant studies were systematically searched by using standardized keywords across three databases. The initial search identified 1,386 references. Of these studies, there were five quantitative studies that met the inclusion criteria and were methodologically appraised using the McMaster Critical Review Form–Quantitative Studies. All studies reported that preschool children with or without disabilities showed significant improvements in handwriting skills after receiving occupational therapy intervention. One study involved collaboration between teachers and occupational therapists in implementing intervention program to preschool students. Collaboration between teachers and occupational therapists was able to provide teachers with tools and skills that they can use to help students, with or without direct presence of occupational therapists. Overall, findings of this review indicated that preschool children could gain improvement in handwriting skills regardless of their conditions after receiving occupational therapy intervention programs.
Evaluation of pre-qualifying interprofessional education (IPE) programmes is important in exploring its effectiveness. The University of the West of England Interprofessional Questionnaire (UWE IPQ) has been rigorously validated, yet few studies using this tool have been published. We aimed to explore the attitudes of final year allied health profession (AHP), midwifery and nursing students towards IPE in this study. Differences between students’ attitudes towards IPE according to gender, undergraduate programmes, academic background and experience were also explored. A total of 103 participants, AHP (n = 10), midwifery (n = 19) and nursing (n = 74) students took part in the study. This study design used a cross-sectional questionnaire survey, and was conducted within the Faculty of Health Sciences, University of Southampton. The validated UWE IPQ was administered to a volunteer sample of final year AHP, nursing and midwifery students in June 2010. Findings revealed that majority of the students rated their communication and teamwork skills (76.7%), interprofessional learning (74.8%) and interprofessional relationships (92.2%) positively, but they held negative attitudes towards interprofessional interactions (69.9%). Significant differences were found between participants’ attitudes towards interprofessional learning in relation to undergraduate programmes (p = 0.01) and prior working experience in health and social care (p = 0.03). IPE has shaped the students’ views regarding their collaborative learning and teamwork. Therefore, introducing IPE initiative from the beginning of the pre-qualifying stage at universities would motivate them to become interprofessional collaborative-ready professionals.
Breast cancer survival prediction can have an extreme effect on selection of best treatment protocols. Many approaches such as statistical or machine learning models have been employed to predict the survival prospects of patients, but newer algorithms such as deep learning can be tested with the aim of improving the models and prediction accuracy. In this study, we used machine learning and deep learning approaches to predict breast cancer survival in 4,902 patient records from the University of Malaya Medical Centre Breast Cancer Registry. The results indicated that the multilayer perceptron (MLP), random forest (RF) and decision tree (DT) classifiers could predict survivorship, respectively, with 88.2 %, 83.3 % and 82.5 % accuracy in the tested samples. Support vector machine (SVM) came out to be lower with 80.5 %. In this study, tumour size turned out to be the most important feature for breast cancer survivability prediction. Both deep learning and machine learning methods produce desirable prediction accuracy, but other factors such as parameter configurations and data transformations affect the accuracy of the predictive model.
MeSH terms: Adult; Aged; Aged, 80 and over; Breast Neoplasms/mortality*; Calibration; Decision Trees; Demography; Female; Humans; Middle Aged; Survival Analysis; Neural Networks (Computer); Young Adult; Support Vector Machine
Citation: Malaysian National Cancer Registry Report 2012-2016. Putrajaya: National Cancer Institute, Ministry of Health, Malaysia; 2019.
Older versions:
1. Lim GCC, Yahaya H, Lim TO. The first report of the National Cancer Registry-cancer incidence in Malaysia 2002. Kuala Lumpur: Clinical Research Centre; Ministry of Health Malaysia; 2003.
http://www.crc.gov.my/documents/report/1st%20National%20Cancer%20Registry.pdf
2. Lim GCC, Yahaya H. Second report of the National Cancer Registry-cancer incidence in Malaysia 2003. Kuala Lumpur: Clinical Research Centre; Ministry of Health Malaysia; 2004.
http://www.crc.gov.my/documents/report/2nd%20National%20Cancer%20Registry.pdf
3. Zainal Ariffin O, Nor Saleha IT. National Cancer Registry Report 2007. Putrajaya: National Cancer Registry, Ministry of Health, Malaysia; 2011.
https://www.dropbox.com/s/0pnx95wpi96rz51/MOH2011_National%20Cancer%20Registry%202007.pdf?dl=0
4. Malaysian National Cancer Registry Report 2007-2011. Putrajaya: National Cancer Institute, Ministry of Health, Malaysia; 2016.
https://drive.google.com/file/d/1pCsJIw0ysFccCMFA0XqzfbYu1OFDRbO_/view
Pomelo pomace (PP) from Malaysia Tambun White (PO52) variety, dried at different temperature under vacuum condition were evaluated. PP was obtained after extraction process and contained high moisture content (MC). Dried PP can be used as an alternative to livestock feed, hence, dried concentrated form was preferable. Nevertheless, drying process significantly affects the physico-chemical properties of a dried product. Therefore, the present study was carried out to discover the effects of drying temperature on the physico-chemical properties (MC, pH, vitamin C, browning index (BI) and total color change) of PP. The PP was dried at different drying temperature (50, 60, 70, 80 and 90°C) using a vacuum drying (VD) oven for 24 hr. The freeze drying (FD) process was used as a control. The result showed the MC gradually decreased with increasing drying temperature, of which similar trend was also observed for the water activity and total soluble solids (TSS). In contrast, pH showed increment in value with elevated drying temperature. Interestingly, at 90°C, vitamin C of VD (30.38 mg / 100 g DW) was higher than FD pomelo pomace (21.10 mg / 100 g DW). BI significantly increased as temperature increased. However, no significant changes were observed for total color change after VD. In conclusion, VD at 90°C showed the most recommended temperature because the composition of quality properties at this temperature was not significantly varied with control. These fundamentals information of pomelo pomace could be the guideline for postharvest technologist and food processing manufacturers for various applications.
In the present research, microalgae protein hydrolysate of Nannochloropsis gaditana (MPH) was extracted via enzymatic hydrolysis using alcalase enzyme. Hydrolysis conditions like (pH, temperature, enzyme concentration and substrate concentration) were optimized by Response Surface Methodology (RSM) using Central Composite Design (CCD). Four range of independent variables namely; pH (7-9), temperature (45-55°C), substrate concentration (2-6 g/L) and enzyme concentration (0.2-0.4 g/L) were used to study the influence of these parameters on the degree of hydrolysis. The CCD consisted of twenty-four experimental points and six replicates of central points with the optimum conditions obtained from this experiment were at pH 8.14, a temperature 51.4°C, a substrate concentration 5.48 g/L and an enzyme concentration 0.26 g/L with maximum degree hydrolysis of 55.76%. All experiments were fixed at 24 hours reaction time. The degree of hydrolysis of MPH was analysed using O-phthaldehyde (OPA) method to quantify the cleavage of peptide bond. The optimized sample was evaluated for its antioxidant activity using 1,1-Diphenyl-2-picrylhydrazyl (DPPH) assay with 52.19% and 2, 2'- azino-bis (ethylbenzthiazoline-6-sulfonic acid (ABTS) assay with 14.13%. The bioactive peptides contained in Nannochloropsis gaditana have the ability to scavenge free radicals and act as good antioxidants.