Browse publications by year: 2024

  1. Waras MN, Zabidi MA, Ismail Z, Sangaralingam M, Gazzali AM, Harun MSR, et al.
    Water Environ Res, 2024 Aug;96(8):e11095.
    PMID: 39114880 DOI: 10.1002/wer.11095
    The Kereh River in Penang, Malaysia, has faced severe pollution for over 40 years due to untreated wastewater from swine farms in Kampung Selamat, discharged via stormwater drains. Despite official claims that all 77 swine farms treat their wastewater to meet regulatory standards, local non-governmental organizations and villagers have challenged this, though their concerns lack scientific backing. This study evaluates the river's water quality by analyzing samples from upstream (US), midstream (MS), and downstream (DS), and from Parit Cina-Parit Besar, a conduit for untreated swine wastewater. Fourteen parameters were measured against Malaysia's National Water Quality Standards (NWQS). Significant differences were found in six parameters: ammonium nitrogen (AN), biochemical oxygen demand (BOD), chemical oxygen demand (COD), dissolved oxygen (DO), total suspended solids (TSS), and oil and grease (OG). While Dunn's post hoc pairwise comparison showed no significant differences among river segments, mean values indicated increased pollution downstream, particularly after the convergence with untreated swine wastewater. River classification worsened, with water quality index dropping from 69.88 ± 11.37 score (Class III) US to 38.49 ± 12.74 and 50.44 ± 3.14 scores (Class IV) MS and downstream, respectively. A significant positive correlation between E. coli and AN (r = 0.71, p 
    MeSH terms: Animals; Animal Husbandry; Environmental Monitoring; Malaysia; Swine; Waste Disposal, Fluid; Water Pollutants, Chemical/analysis; Biological Oxygen Demand Analysis; Water Quality*; Farms
  2. Huang HJ, Tian KL, Wong SQ, Lian NX, Wang J, Sun HJ, et al.
    Chemistry, 2024 Aug 07.
    PMID: 39109456 DOI: 10.1002/chem.202402667
    A novel room-temperature liquid crystal of tetraphenylethylene derivative (TPE-DHAB) was synthesized using an ionic self-assembly strategy. The TPE-DHAB complex exhibits typical aggregation-induced emission properties and a unique helical supramolecular structure. Moreover, the generation and handedness inversion of circularly polarized luminescence (CPL) can be achieved through further chiral solvation, providing a facile approach to fabricate room-temperature liquid crystalline materials with controllable supramolecular structures and tunable CPL properties through a synergistic strategy of ionic self-assembly and chiral solvation process.
  3. Sani AR, Zin CS
    J Psychoactive Drugs, 2024 Aug 05.
    PMID: 39101200 DOI: 10.1080/02791072.2024.2387602
    This study examined the factors associated with a high risk of opioid misuse among patients receiving opioid treatment for their non-cancer pain in Malaysian pain clinics. The Screener and Opioid Assessment for Patients with Pain-Revised (SOAPP-R), a validated instrument for predicting the risk of aberrant drug-related behaviors, were used as a proxy to indicate risk of opioid misuse. Data analysis was stratified into high-risk and low-risk patient groups. Patient factors assessed included pain intensity, pain interference with daily activities, and health-related quality of life. Prescription opioid exposure was examined via patient medical and prescription records review. Among the 61 patients recruited, 62.3% scored ≥18 on the SOAPP-R, which indicates a high risk for opioid misuse. Factors associated with a high risk of opioid misuse were found to be high level of pain interference with daily activities, poorer mental health, and younger age. High-risk patients were found to be prescribed a lower mean daily opioid dose of <20 mg/day compared to low-risk patients (20-49 mg/day). This highlights the need for further research to distinguish aberrant drug-related behaviors due to inadequate pain management from that of actual prescription opioid misuse among non-cancer pain patients attending pain clinics.
  4. Tajudin MABA, Madaniyazi L, Seposo X, Sahani M, Tobías A, Latif MT, et al.
    Int J Epidemiol, 2024 Jun 12;53(4).
    PMID: 39096096 DOI: 10.1093/ije/dyae102
    BACKGROUND: Biomass burning (BB) is a major source of air pollution and particulate matter (PM) in Southeast Asia. However, the health effects of PM smaller than 10 µm (PM10) originating from BB may differ from those of other sources. This study aimed to estimate the short-term association of PM10 from BB with respiratory and cardiovascular hospital admissions in Peninsular Malaysia, a region often exposed to BB events.

    METHODS: We obtained and analyzed daily data on hospital admissions, PM10 levels and BB days from five districts from 2005 to 2015. We identified BB days by evaluating the BB hotspots and backward wind trajectories. We estimated PM10 attributable to BB from the excess of the moving average of PM10 during days without BB hotspots. We fitted time-series quasi-Poisson regression models for each district and pooled them using meta-analyses. We adjusted for potential confounders and examined the lagged effects up to 3 days, and potential effect modification by age and sex.

    RESULTS: We analyzed 210 960 respiratory and 178 952 cardiovascular admissions. Almost 50% of days were identified as BB days, with a mean PM10 level of 53.1 µg/m3 during BB days and 40.1 µg/m3 during normal days. A 10 µg/m3 increment in PM10 from BB was associated with a 0.44% (95% CI: 0.06, 0.82%) increase in respiratory admissions at lag 0-1, with a stronger association in adults aged 15-64 years and females. We did not see any significant associations for cardiovascular admissions.

    CONCLUSIONS: Our findings suggest that short-term exposure to PM10 from BB increased the risk of respiratory hospitalizations in Peninsular Malaysia.

    MeSH terms: Adolescent; Adult; Aged; Child; Child, Preschool; Environmental Exposure/adverse effects; Environmental Exposure/statistics & numerical data; Female; Humans; Infant; Infant, Newborn; Malaysia/epidemiology; Male; Middle Aged; Biomass*; Young Adult
  5. Chowdhury K, Sinha S, Ahmad R, Lugova H, Mehta M, Kumar S, et al.
    Cureus, 2024 Jul;16(7):e65808.
    PMID: 39092382 DOI: 10.7759/cureus.65808
    Cardiometabolic syndrome (CMS), type 2 diabetes mellitus (T2DM), and cardiovascular diseases are among the major altruists to the international liability of disease. The lifestyle and dietary changes attributable to economic growth have resulted in an epidemiological transition towards non-communicable diseases (NCDs) as the leading causes of death. Low- and middle-income countries (LMICs) bear a more substantial disease burden due to limited healthcare sector capacities to address the rapidly growing number of chronic disease patients. The purpose of this narrative review paper was to explore the interrelationships between CMS, T2DM, and cardiovascular impairments in the context of NCDs, as well as major preventative and control interventions. The role of insulin resistance, hyperglycemia, and dyslipidemia in the pathogenesis of T2DM and the development of severe cardiovascular impairments was highlighted. This paper elaborated on the pivotal role of lifestyle modifications, such as healthy diets and physical activity, as cornerstones of addressing the epidemics of metabolic diseases. Foods high in calories, refined sugar, red meat, and processed and ready-to-eat meals were associated with an amplified risk of CMS and T2DM. In contrast, diets based on fruits, legumes, vegetables, and whole grain, home-cooked foods demonstrated protective effects against metabolic diseases. Additionally, the role of a psychological and behavioral approach in addressing metabolic diseases was highlighted, especially regarding its impact on patient empowerment and the patient-centered approach to preventative and therapeutic interventions.
  6. Kumbhar PS, Kamble V, Vishwas S, Kumbhar P, Kolekar K, Gupta G, et al.
    Drug Deliv Transl Res, 2024 Sep;14(9):2325-2344.
    PMID: 38758498 DOI: 10.1007/s13346-024-01607-9
    Skin cancer remains one of the most prominent types of cancer. Melanoma and non-melanoma skin cancer are commonly found together, with melanoma being the more deadly type. Skin cancer can be effectively treated with chemotherapy, which mostly uses small molecular medicines, phytoceuticals, and biomacromolecules. Topical delivery of these therapeutics is a non-invasive way that might be useful in effectively managing skin cancer. Different skin barriers, however, presented a major obstacle to topical cargo administration. Transferosomes have demonstrated significant potential in topical delivery by improving cargo penetration through the circumvention of diverse skin barriers. Additionally, the transferosome-based gel can prolong the residence of drug on the skin, lowering the frequency of doses and their associated side effects. However, the choice of appropriate transferosome compositions, such as phospholipids and edge activators, and fabrication technique are crucial for achieving improved entrapment efficiency, penetration, and regulated particle size. The present review discusses skin cancer overview, current treatment strategies for skin cancer and their drawbacks. Topical drug delivery against skin cancer is also covered, along with the difficulties associated with it and the importance of transferosomes in avoiding these difficulties. Additionally, a summary of transferosome compositions and fabrication methods is provided. Furthermore, topical delivery of small molecular drugs, phytoceuticals, and biomacromolecules using transferosomes and transferosomes-based gel in treating skin cancer is discussed. Thus, transferosomes can be a significant option in the topical delivery of drugs to manage skin cancer efficiently.
    MeSH terms: Administration, Cutaneous; Animals; Humans; Liposomes*; Skin Absorption; Drug Delivery Systems
  7. Feng A, O'Neill S, Rostain AL
    J Atten Disord, 2024 Jul 31.
    PMID: 39082427 DOI: 10.1177/10870547241264113
    OBJECTIVE: Rates of ADHD are lowest among Asian American children (1-6.1%) compared to all other major ethnic and racial groups in the US, but there is limited literature on reasons for the disparity in estimated prevalence rates.

    METHOD: We conducted a narrative review to integrate the literature on ADHD in children in Asian countries with that on ADHD among Asian American youth to highlight potential explanations for disparities in ADHD diagnosis and treatment among Asian American children relative to other racial and ethnic groups.

    RESULTS: Factors possibly contributing to the low estimated prevalence rates of ADHD among Asian American children include: a higher proportion of Inattentive ADHD presentation among Chinese, Malaysian, and Indian children; racial bias and the influence of the Model Minority Myth; cultural differences in classroom identification; mental health stigma in Asian American communities; parent perception of ADHD as misbehavior rather than a neurodevelopmental disorder; and parent support for children's academic activities that may mask impairment.

    CONCLUSION: We offer recommendations to inform individual and community-level psychoeducation, and new directions for research to address this health disparity.

  8. Ahip SS, Ting CY, Abdillah MAB, Tan YJ, Sabri SAB, Theou O, et al.
    J Frailty Aging, 2024;13(3):313-318.
    PMID: 39082778 DOI: 10.14283/jfa.2024.42
    BACKGROUND: This study aimed to evaluate the implementation stage of Malaysia's GeKo-Integrated Service Delivery (ISD) model for frailty management in primary care and explore its effectiveness in improving frailty scores.

    METHODS: The implementation stage of Malaysia's first three GeKo- ISD clinics was assessed using the WHO-ICOPE (Integrated Care of the Older Persons) scorecard. This involved evaluating documents related to the GeKo services and conducting in-depth interviews with key informants identified from those documents. The efficacy of GeKo-ISD was assessed by documenting the change in mean frailty scores between baseline and 3 months post intervention, measured by the Pictorial Fit Frail Scale Malay Version (PFFS-M), in patients who received GeKo-ISD care from October 2022 to April 2023.

    RESULTS: All three GeKo clinics achieved the sustaining implementation level, scoring a total of 50 out of 52. The paired t-test reported a significant reduction (p= 0.001) in the PFFS-M scores from baseline to 3 months after the GeKo-ISD intervention. The mean (SD) scores were 8.6 (4.6) at baseline and 7.0 (4.1) at 3 months post-intervention.

    CONCLUSION: GeKo-ISD is a comprehensive approach of integrated care for older people, leveraging existing public funded primary care infrastructure. It shows promise, was impacted by the pandemic but now, with support from the government, exists in 32 centers across one state in Malaysia.

    MeSH terms: Aged; Aged, 80 and over; Female; Health Services for the Aged/organization & administration; Humans; Malaysia; Male; Geriatric Assessment/methods; Frail Elderly*
  9. Anam C, Sutanto H, Amilia R, Marini R, Barokah SN, Osman ND, et al.
    Phys Eng Sci Med, 2024 Dec;47(4):1525-1535.
    PMID: 39083162 DOI: 10.1007/s13246-024-01465-2
    The aim of this study was to evaluate the point doses using a distribution of the size-specific dose estimate (SSDE) from axial CT images of in-house phantoms having diameters from 8 to 40 cm. In-house phantoms made of polyester-resin (PESR) mixed with methyl ethyl ketone peroxide (MEKP) were used. The phantoms were built with different diameter sizes of 8, 16, 24, 32, and 40 cm. The phantoms were scanned by Siemens a SOMATOM Perspective-128 slice CT scanner with constant input parameters. The point doses were interpolated from the central SSDE (SSDEc) and the peripheral SSDE (SSDEp). The SSDEc and SSDEp were calculated from the SSDE with h- and k-factors. The point doses were compared to the direct measurements using the nanoDot™ optically-stimulated luminescence dosimeter (OSLD) in dedicated holes on the phantoms. It was found that the point dose decreases as the phantom diameter increased. The doses obtained using two approaches differed by 11% on average. The highest difference was 40% and the lowest difference was
    MeSH terms: Dose-Response Relationship, Radiation; Humans; Radiation Dosage*; Radiometry; Tomography, X-Ray Computed; Phantoms, Imaging*
  10. Wong ZY, Teo CY, Fiona Wong YQ, Ng KT, Lim SK
    PLoS One, 2024;19(7):e0307981.
    PMID: 39083488 DOI: 10.1371/journal.pone.0307981
    OBJECTIVE: There is limited evidence on which immunosuppressive agents produce the best outcomes for adult patients with steroid-dependent or frequently relapsing nephrotic syndrome (SDNS/FRNS). This review compares the remission rate and adverse effects of various immunosuppressants used.

    METHODS: Studies of adult patients with biopsy-proven SDNS/FRNS, administered any immunosuppressive agents and reported complete remission results as one of the clinical outcomes were included. Articles were independently screened by two researchers. ROBINS-I was used for risk of bias assessment. Random-effects model was used for statistical analysis and corresponding 95% confidence intervals (CIs) were calculated.

    RESULTS: 574 patients across 28 studies were included in the analysis. Patients receiving rituximab have a complete remission rate of 89% (95% CI = 83% to 94%; τ2 = 0.0070; I2 = 62%; overall p < 0.01, low certainty) and adverse event rate of 0.26, cyclosporine (CR 40%; 95% CI = 21% to 59%; τ2 = 0.0205; I2 = 55%; overall p = 0.08, low certainty), tacrolimus (CR 84%; 95% CI = 70% to 98%; τ2 = 0.0060; I2 = 33%; overall p = 0.21, moderate certainty), mycophenolate mofetil (CR 82%; 95% CI = 74% to 90%; τ2 < 0.0001; I2 = 15%; overall p = 0.32, moderate certainty) and cyclophosphamide (CR 79%; 95% CI = 69% to 89%; τ2 = 0; I2 = 0%; overall p = 0.52, moderate certainty).

    CONCLUSION: Among the commonly used immunosuppressive agents, only rituximab has a statistically significant effect in achieving complete remission among patients with SDNS/FRNS and has a relatively good safety profile, but this is limited by low quality of evidence with high degree of heterogeneity causing a lack of statistical power.

    MeSH terms: Rituximab/adverse effects; Rituximab/therapeutic use; Adult; Humans; Immunosuppression; Recurrence; Remission Induction; Steroids/adverse effects; Steroids/therapeutic use; Treatment Outcome
  11. Chai SS, Ting SH, Goh KL, Chang YHR, Wee BL, Novita D, et al.
    PLoS One, 2024;19(7):e0307262.
    PMID: 39083489 DOI: 10.1371/journal.pone.0307262
    Amidst the digital transformation of education, the essence of the human touch in online teaching remains pivotal. Despite growing literature, there remains a significant gap in understanding how the human element in online teaching directly influences student engagement and learning outcomes, especially in diverse educational contexts. This study develops a quantifiable index capturing the essence of humanized online teaching and investigates the determinants influencing this humanization. Additionally, an index encapsulating students' online learning experiences, as perceived by their instructors, has been constructed. Bridging these indices, the research unravels the intricate relationship between the humanization of online teaching and the resulting student experiences in the virtual realm. Sourced from a self-constructed questionnaire and encompassing responses from 152 instructors across 22 Malaysian institutions, the data revealed an average incorporation of 81.38% humanized online teaching elements. Key determinants, such as subject matter, teaching experience, Internet quality, and platform choices, emerged as significant influences. A regression model showed approximately 31.7% (R-squared = 0.317, p<0.001) of the variation in the dependent variable. A significant moderate positive correlation (r = 0.423, p<0.001) between the Humanized Online Teaching Index and the Students' Online Learning Experiences Index highlights the intertwined nature of humanized instructional methodologies and enhanced student engagement in online settings. Though contextualised during the coronavirus disease-2019 pandemic, the study's implications transcend the immediate circumstances, offering transformative insights for future online teaching methodologies and enhancing student experiences in the evolving digital age.
    MeSH terms: Adult; Female; Humans; Learning; Malaysia; Male; Surveys and Questionnaires; Students/psychology; Teaching; Internet
  12. Vo DH, Vo AT, Ho CM
    PLoS One, 2024;19(7):e0304678.
    PMID: 39083555 DOI: 10.1371/journal.pone.0304678
    Human capital is a nation's primary source of inner strength to achieve sustainable economic growth and development. Meanwhile, income inequality is a critical issue preventing sustainable economic growth and social transformation, especially in developing countries. This paper investigates the effect of human capital on income inequality in both the short and long term using the mean group, pooled mean group, and threshold regressions for the ASEAN-7 (including Indonesia, Laos, Malaysia, the Philippines, Singapore, Thailand, and Vietnam) from 1992 to 2018. The paper develops a theoretical linkage between human capital and income inequality by combining the learning theory and the Kuznets hypothesis. This linkage is then tested using data from the ASEAN countries. Findings from the paper indicate that human capital reduces income inequality in the short run in the ASEAN countries. However, the effect is reverted in the long run, suggesting that human capital may increase the income gap in these countries. Particularly, the inverted U-shaped relationship between human capital and income inequality is established for the ASEAN countries whose GDP per capita is lower than USD 8.2 thousand per year. In contrast, the U-shaped relationship is found for the countries with income per capital of more than USD 8.2 thousand. All these findings suggest that social policies targeting reducing income inequality should be prioritized and stay at the centre of any economic policies to achieve sustainable economic growth and development in the ASEAN countries.
    MeSH terms: Developing Countries/economics; Humans; Income*; Socioeconomic Factors*; Economic Development
  13. Modrzejewska J, Todd J, Czepczor-Bernat K, Modrzejewska A, Wyszomirska J, Swami V
    Body Image, 2024 Dec;51:101771.
    PMID: 39083985 DOI: 10.1016/j.bodyim.2024.101771
    The 13-item Body Acceptance by Others Scale-2 (BAOS-2) measures generalised perceptions of body acceptance by others. Here, we examined the psychometric properties of a novel Polish translation of the BAOS-2. A sample of 1183 adults (898 women, 285 men; age M = 28.98) from Poland completed a Polish version of the BAOS-2, alongside measures of body appreciation, pressure to conform to appearance ideals, self-compassion, self-esteem, and impression management. Exploratory and confirmatory factor analysis identified a stable unidimensional model of the Polish BAOS-2, with all 13 items retained. Additionally, the Polish BAOS-2 evidenced complete invariance (i.e., through to latent mean invariance) across gender identity. Scores on the BAOS-2 evidenced adequate composite reliability and, in a retest subsample (n = 260), showed complete invariance across four weeks. Evidence of convergent, criterion-related, discriminant, and incremental validity was very strong when based on observed BAOS-2 scores, but weaker when based on latent BAOS-2 scores. Whether examined at the observed or latent level, greater body acceptance by others was consistently and strongly associated with higher body appreciation. These results suggest that the psychometric properties of the Polish BAOS-2 are robust and that this instrument can be confidently utilised in future research with Polish adults.
    MeSH terms: Adolescent; Adult; Female; Humans; Male; Middle Aged; Poland; Psychometrics*; Surveys and Questionnaires; Self Concept*; Translations; Reproducibility of Results; Young Adult
  14. Aravindan D, Alagan AA, Heidelberg T, Cheng SF, Duali Hussen RS
    Carbohydr Res, 2024 Jul 29;543:109224.
    PMID: 39084163 DOI: 10.1016/j.carres.2024.109224
    Glycolipids incorporating positive charges, mediated by an imidazolium cation, have shown potential for effective formulation of vesicular drug carriers, reflecting repulsive electrostatic forces, promoting the formation of nanosized assemblies and preventing unwanted Oswald ripening (Goh et al. (2019), ACS Omega 4, 17,039). Our continuous development of an assembly-based drug delivery system prompted us to investigate a pH-sensitive analogue, leading to the synthesis of a 6-amino-Guerbet glycoside. However, in contrast to the imidazolium counterpart, the amine-mediated charge increased the intermolecular cohesions, furnishing bigger assemblies instead, which further increased upon introduction of acid. Moreover, assemblies exhibited a significantly reduced positive charge density. It is concluded that strong proton-initiated hydrogen bonding between amino groups provide cohesive head group interactions overcompensating possible repulsive charge interactions. While this behavior invalidates the application of the amino-glucoside as dispersing agent for the formulation of small vesicles, it potentially paves a route towards enhanced vesicle stability.
  15. Xie W, Yao Z, Yuan Y, Too J, Li F, Wang H, et al.
    Genomics, 2024 Jul 29.
    PMID: 39084477 DOI: 10.1016/j.ygeno.2024.110906
    Enhancers are crucial in gene expression regulation, dictating the specificity and timing of transcriptional activity, which highlights the importance of their identification for unravelling the intricacies of genetic regulation. Therefore, it is critical to identify enhancers and their strengths. Repeated sequences in the genome are repeats of the same or symmetrical fragments. There has been a great deal of evidence that repetitive sequences contain enormous amounts of genetic information. Thus, We introduce the W2V-Repeated Index, designed to identify enhancer sequence fragments and evaluates their strength through the analysis of repeated K-mer sequences in enhancer regions. Utilizing the word2vector algorithm for numerical conversion and Manta Ray Foraging Optimization for feature selection, this method effectively captures the frequency and distribution of K-mer sequences. By concentrating on repeated K-mer sequences, it minimizes computational complexity and facilitates the analysis of larger K values. Experiments indicate that our method performs better than all other advanced methods on almost all indicators.
  16. Kamath AP, Nayak PG, John J, Mutalik S, Balaraman AK, Krishnadas N
    Neuropharmacology, 2024 Jul 29.
    PMID: 39084596 DOI: 10.1016/j.neuropharm.2024.110096
    Neurological disorders pose a huge worldwide challenge to the healthcare system, necessitating innovative strategies for targeted drug delivery to the central nervous system. Alzheimer's disease (AD) is an untreatable neurodegenerative condition characterized by dementia and alterations in a patient's physiological and mental states. Since ancient times, medicinal plants have been an important source of bioactive phytochemicals with immense therapeutic potential. This review investigates new and safer alternatives for prevention and treatment of disease related to inevitable side effects associated with synthetic compounds. This review examines how nanotechnology can help in enhancing the delivery of neuroprotective phytochemicals in AD. Nevertheless, despite their remarkable neuroprotective properties, these natural products often have poor therapeutic efficacy due to low bioavailability, limited solubility and imperfect blood brain barrier (BBB) penetration. Nanotechnology produces personalized drug delivery systems which are necessary for solving such problems. In overcoming these challenges, nanotechnology might be employed as a way forward whereby customized medication delivery systems would be established as a result. The use of nanocarriers in the design and application of important phytochemicals is highlighted by this review, which indicate potential for revolutionizing neuroprotective drug delivery. We also explore the complications and possibilities of using nanocarriers to supply nutraceuticals and improve patients' standard of living, and preclinical as well as clinical investigations displaying that these techniques are effective in mitigating neurodegenerative diseases. In order to fight brain diseases and improve patient's health, scientists and doctors can employ nanotechnology with its possible therapeutic interventions.
  17. Peng TL, Syazwan SA, Hamdan RH, Najwa NS, Ramli MF, Harshiny N, et al.
    Pestic Biochem Physiol, 2024 Aug;203:105982.
    PMID: 39084787 DOI: 10.1016/j.pestbp.2024.105982
    The tropical climate in Malaysia provides an ideal environment for the rapid proliferation of Aedes mosquitoes, notably Aedes aegypti and Aedes albopictus, prominent vectors of dengue fever. Alarmingly, these species are increasingly developing resistance to conventional pesticides. This study aimed to evaluate the efficacy of Metarhizium anisopliae isolate HSAH5 spores, specifically on conidia (CO) and blastospores (BL), against Ae. albopictus larvae. The study centered on evaluating their pathogenic effects and the resultant changes in protein expression. Spore suspensions with varying concentrations were prepared for larvicidal bioassays, and protein expressions were analysed using liquid chromatography-mass spectrometry. Subsequently, protein annotation and network analysis were conducted to elucidate infection mechanisms and the proteomic response. Based on the lethal concentrations and time frames, CO exhibited faster larval mortality than BL at lower concentrations. Despite this, both spore types demonstrated comparable overall pathogenic effects. Results from the proteomic profiling revealed 150 proteins with varied expressions following exposure to Ae. albopictus extract, shedding light on distinct infection strategies between the spores. Gene Ontology enrichment and network analysis illustrated the diverse metabolic adaptations of M. anisopliae and interactions with mosquito larvae. This highlighted the complexity of host-pathogen dynamics and the significance of biosynthetic processes, energy storage, and cellular interaction pathways in disease progression. The BL network, consisting 80 proteins and 74 connections, demonstrates the intricate fungal mechanisms triggered by host stimuli. Conversely, the CO network, though smaller, displayed notable interconnectivity and concentrated involvement at the cell periphery, suggesting a deliberate strategy for initial host contact. This study offers valuable insights into proteome dynamics of M. anisopliae's BL and CO for managing mosquito populations and combating disease transmission, thereby significantly advancing public health and environmental conservation efforts.
    MeSH terms: Animals; Fungal Proteins/genetics; Fungal Proteins/metabolism; Mosquito Control/methods; Pest Control, Biological; Spores, Fungal*; Virulence
  18. Xu X, Deng S, Essawy H, Lee SH, Lum WC, Zhou X, et al.
    Int J Biol Macromol, 2024 Oct;277(Pt 1):133784.
    PMID: 39084972 DOI: 10.1016/j.ijbiomac.2024.133784
    In this study, various chitosan-based films such as chitosan (C), chitosan-condensed tannin (CT), chitosan-casein (CC), and chitosan-casein-condensed tannin (CCT) films were prepared for the purpose of food packaging. In order to improve the hydrophobicity of these films, carnauba wax was blended into CCT to produce CCTW film. Properties such as morphology, UV resistance, water solubility, barrier performance, tensile strength, antioxidant, antibacterial and its performance as food packaging were evaluated. Compared with other chitosan-based films, CCTW films exhibited higher UV resistance, tensile strength, thermal stability and hydrophobicity. The addition of both condensed tannin and carnauba wax has significantly decreased the water vapor and oxygen permeability of the CCTW films. The CCTW films were proved capable of repelling most daily consuming liquids. Besides, CCTW films displayed outstanding free radical scavenging rate and antibacterial properties. Meanwhile, bananas wrapped with CCTW films remained fresh for seven days without any mold growth and outperformed other types of films. Apart from that, the CCTW films also showed biodegradable characteristics after exposure to Penicillium sp. These distinguished characteristics made the CCTW films a promising packaging material for long-term food storage.
    MeSH terms: Permeability; Solubility; Steam; Tannins/chemistry; Tensile Strength; Hydrophobic and Hydrophilic Interactions
  19. Potter RE, Ertel M, Dollard MF, Leka S, Jain A, Lerouge L, et al.
    Ind Health, 2024 Nov 26;62(6):353-366.
    PMID: 39085135 DOI: 10.2486/indhealth.2024-0092
    Worker psychological health is a significant global imperative that requires national policy action and stakeholder engagement. While national policy is a critical lever for improving worker psychological health, some countries are more progressive than others in relation to policy development and/or implementation. At the Joint Congress of the International Commission on Occupational Health, Scientific Committee on Work Organization and Psychosocial Factors and the Asia Pacific Academy for Psychosocial Factors at Work in Tokyo (September 2023), a Global Roundtable was held that to initiate international dialogue and knowledge exchange about national policy approaches for work-related psychological health. The Global Roundtable involved experts from diverse regions alongside an engaged audience of congress attendees and facilitators. Qualitative data were analysed against the five components of the National Policy Index tool comprising, policy priority, specific laws, nation-wide initiatives, sector-oriented initiatives, national survey and/or studies. Analysis revealed that while work-related psychological health is a policy priority across many countries, at the same time, there are global gaps in both legislation specificity and active regulation across different countries. For future policy development across countries, it will be beneficial to continue and deepen international discourse and for countries to share their approaches with others.
    MeSH terms: Health Policy*; Humans; Mental Health*; Policy Making; Global Health; Occupational Health*; Workplace/psychology
  20. Mohd Kasim VNK, Lee YZ, Bakrin IH, Hussain MK, Israf DA, Shaari K, et al.
    Sci Rep, 2024 Jul 31;14(1):17623.
    PMID: 39085287 DOI: 10.1038/s41598-024-66601-0
    Atopic dermatitis (AD) is a chronic, allergic inflammatory skin disorder that lacks a definite cure. Using a mouse DNCB-induced AD-like skin lesions model, this study evaluated the potential therapeutic utility of tHGA as an oral and topical treatment for AD. Male BALB/c mice were sensitised and challenged with 1% and 0.5% DNCB on their shaved dorsal skin. Mice in the treatment group were administered tHGA (20, 40, and 80 mg/kg) orally three times per week for 2 weeks, or tHGA (0.2%, 1%, and 5%) topically once daily for 12 days. On day 34, the mice were euthanized, and blood and dorsal skin samples were obtained for analysis. All doses of orally and topically administered tHGA significantly improved scratching, epidermal thickness, blood eosinophilia and mast cell infiltration. There was a minor discrepancy between the two routes of administration, with orally treated tHGA showing significant reductions in Scoring of Atopic Dermatitis (SCORAD), tissue eosinophil infiltration, serum IgE and skin IL-4 levels with treatment of 40 and 80 mg/kg tHGA, whereas topically applied tHGA showed significant reductions in all dosages. These findings suggest that tHGA exhibited therapeutic potential for AD as both oral and topical treatment ameliorates AD-like symptoms in the murine model.
    MeSH terms: Acetophenones/administration & dosage; Acetophenones/pharmacology; Acetophenones/therapeutic use; Administration, Oral; Administration, Topical*; Animals; Dinitrochlorobenzene*; Disease Models, Animal; Eosinophils/drug effects; Male; Mast Cells/drug effects; Mice, Inbred BALB C*; Interleukin-4/metabolism; Mice
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