Browse publications by year: 2024

  1. Gupta B, Parul, Lee Y, Soo JZ, Adhikari S, Cheong Lem OL, et al.
    Small Sci, 2024 Dec;4(12):2400167.
    PMID: 40213485 DOI: 10.1002/smsc.202400167
    III-V semiconductors are favoured photo absorber materials for solar energy conversion due to their ideal bandgap, yet their high-cost hinders widespread adoption. Utilizing thin films of these semiconductors presents a viable way to address the cost-related challenges. Here, a novel mechanical exfoliation technique is demonstrated, also known as controlled spalling, as a cost-effective and facile way to obtain thin films of III-V semiconductors. As a proof of concept, 15 μm thick InP films are successfully exfoliated from their original wafers. Thorough characterization using cathodoluminescence and photoluminescence spectroscopy confirms that the opto-electronic properties of the exfoliated InP films remain unaffected. Utilizing these InP thin films, InP thin-film heterojunction solar cells with efficiencies exceeding 13% are demonstrated. Additionally, InP photoanodes are fabricated by integrating NiFeOOH catalyst onto these InP thin-film solar cells, achieving an impressive photocurrent density of 19.3 mA cm-2 at 1.23 V versus reversible hydrogen electrode, along with an applied bias photon-to-current efficiency of ≈4%. Overall, this study showcases the efficacy of controlled spalling in advancing economically viable and efficient III-V semiconductor-based solar energy conversion devices.
  2. Amin Nordin FD, Omar A, Kamarudin B, Simpson T, Abdul Jalil J, Pung YF
    Mol Genet Metab Rep, 2024 Sep;40:101094.
    PMID: 40206842 DOI: 10.1016/j.ymgmr.2024.101094
    Broad biochemical complexity and frequent overlapping clinical symptoms of inborn errors of metabolism (IEM), especially in energy-deficient patients, make accurate diagnosis difficult. In recent years, whole exome sequencing (WES), a comprehensive protein coding genetic test, has been used to diagnose patients at the molecular level. This study aims to evaluate the potential of WES in diagnosing energy-deficient IEM patients with limited biochemical findings and to identify common symptoms patterns in reported cases. Articles were identified using a combination of search terms in online databases (Science Direct, PubMed Central and Wiley). English-language case reports citing WES in the diagnosis of energy-deficient IEM patients were reviewed. This systematic review was conducted and reported using the 'Preferred Reporting Items for Systematic Reviews and Meta-Analyses' checklist. The quality and risk of bias were assessed using Joanna Briggs Institute critical appraisal tool. A total of 37 studies comprising of 54 case reports were included in this review. The median age of the patients was 0.4 years, with 55.6% being male and 44.4% being female. A total of 33 mutant genes were reported and they related to either metabolism or mitochondrial function. WES was able to identify mutations in 53 of 54 cases reported. The diagnosis of energy-deficient IEM patients is crucial, particularly given the challenging range of diverse clinical symptoms they present. The high accuracy of the WES technique appears to improve the diagnostic process. Further research defining more detailed guidelines is needed to engage with this rare set of genetic diseases.
  3. Shutari H, Ibrahim T, Bin Mohd Nor N, Alharthi YZ, Abdulrab H
    Heliyon, 2024 Jun 30;10(12):e32032.
    PMID: 39183878 DOI: 10.1016/j.heliyon.2024.e32032
    The growing interest in wind power technology is motivating researchers and decision-makers to focus on maximizing wind energy extraction and enhancing the quality of power integrated into the grid. Over the past decades, significant advancements have been made in Wind Energy Conversion Systems (WECS), such as moving to variable speed wind turbines (VSWT), using various generator types, and interfacing with many power electronic converter topologies. Recently, the majority of wind turbine industries have adopted the VSWT, which is based on the permanent magnet synchronous generator (PMSG) and incorporates a fully controlled power electronic converter (FCPEC) topology due to its notable features of full controllability, ultimately enhancing the efficiency and power quality of the WECS. This paper presents a concise overview of the PMSG-VSWT system and comprehensively reviews the most recent control approaches developed for the FCPEC that play a crucial role in the operation and performance of the PMSG-VSWT system. The paper begins with a comprehensive review of the Maximum Power Extraction Algorithms (MPEA) used in the PMSG-VSWT system, as reported in esteemed research articles over recent years. It investigates the fundamental concepts of each MPEA, examining their advantages and disadvantages, providing critical comparisons, highlighting related work, and discussing the advancements achieved in this field. Subsequently, the paper reviews the prevalent control schemes for the Grid-Side Inverter and Machine-Side Rectifier (GSI/MSR) in the FCPEC. It covers common control approaches such as vector control, direct control, sliding mode control, and model productive control, including modern and intelligent techniques. Additionally, the paper details recent improvements and approaches adopted to address challenges in these common schemes, involving optimizing algorithms and adaptive techniques. The paper provides essential insights into trends, improvements, and challenges in the domain and acts as a crucial reference for researchers working with PMSG-VSWT systems.
  4. Isayev K, Rasulov O, Sadigova N
    Open Res Eur, 2024;4:247.
    PMID: 40196757 DOI: 10.12688/openreseurope.18695.2
    BACKGROUND: Currently, a wide variety of silicon photomultipliers (SiPMs) are available, each designed for specific applications in fields such as science, medicine, and industry. Advances in production technology have led to the development of more sensitive and efficient photodiodes, which are critical for applications requiring precision, such as medical imaging.

    METHODS: A research group has been working on designing a highly sensitive photodiode to enhance the capabilities of next-generation of hybrid positron emission tomography (PET) and magnetic resonance imaging (MRI) scanners. This involves integrating micropixel avalanche photodiodes (MAPDs) to improve image resolution. The chosen design features deep-immersion MAPDs with a pixel size of 12 microns and a density of 1000 pixels per mm 2, allowing for high-detail photon detection. The 4x4 mm 2 active area is optimized to balance sensitivity and size for high-resolution medical imaging. To produce these photodiodes, the group has outlined a production plan involving 300 mm silicon wafers grown using multiple techniques to enhance material properties. The Malaysian Institute of Microelectronic Systems (MIMOS), renowned for its expertise in optical microelectronics, was selected as the production center. With MIMOS' state-of-the-art facilities, the project aims to meet stringent medical diagnostics standards.

    RESULTS: The experimental results demonstrated that the MAPD-3NM (MAPD design with 12 microns pixel size) photodiode achieved an amplification factor 1.8 times greater than the MAPD-3NK (MAPD design with 10 microns pixel size) under optimal conditions. The both samples size was 4x4 square mm. Its overvoltage range increased by 100%, reaching 4 V, enhancing photon detection and amplification. The MAPD-3NM also showed a significant reduction in dark current, about 3.5 times lower than the MAPD-3NK, improving performance in low-light environments. Additionally, the MAPD-3NM had a capacitance of 200 pF compared to 176 pF for the MAPD-3NK, contributing to its superior performance. These improvements make the MAPD-3NM more efficient and sensitive for scientific and medical applications.

    CONCLUSIONS: This project represents a major advancement in photodetector technology for medical diagnostics, aiming to develop more accurate and efficient PET-MRI scanners that enhance patient outcomes with improved imaging capabilities.

  5. Al-Mazedi MS, Rajan R, Al-Herz A, Alsaber A, Al-Jarallah M, Dashti R, et al.
    Oman Med J, 2024 Sep;39(5):e671.
    PMID: 40196802 DOI: 10.5001/omj.2024.101
    OBJECTIVES: Hemoglobin (Hb) level and its relation to rheumatoid arthritis (RA) is multifactorial. The primary aim of this study was to examine the association between Hb levels and disease activity in patients with RA.

    METHODS: This retrospective study obtained data from adult RA patients with Hb reports from the Kuwait Registry for Rheumatic Diseases. Patients were recruited from four public hospitals in Kuwait between February 2013 and February 2022. The cohort was stratified into two groups: Hb ≤ 110 g/L and Hb > 110 g/L. Demographic, treatment, clinical, and laboratory characteristics were used to compare the two Hb groups. Multivariate and univariate statistical analyses were used to analyze the data.

    RESULTS: The total number of patients visited (Nv) was 11 393 and consecutive patients with RA diagnoses and Hb data (Np) were 1584. Both Nv and Np were included in the study. Of these, 72.5% (n = 8260) had high Hb levels and 27.5% (n = 3133) had low Hb levels. The average age of the cohort was 55.9 ± 12.5 years. Logistic regression analysis revealed that a greater number of non-Kuwaiti patients had anemia than Kuwaiti patients [adjusted odds ratio (aOR) = 1.34, 95% CI: 1.16-1.56; p < 0.001). Patients who received biologic treatment were more likely to be non-anemic [aOR = 1.33, 95% CI: 1.23-1.45; p < 0.001). Additionally, the study demonstrated that patients with anemia had greater odds of acquiring Disease Activity Score -28 joint count (DAS-28) ≥ 3.2 versus DAS-28 < 3.2 [aOR = 0.74, 95% CI: 0.61-0.90; p = 0.002].

    CONCLUSIONS: Lower Hb levels in RA are an independent predictor of disease activity.

  6. Chong KH, Suesse T, Cross PL, Ryan ST, Aadland E, Aoko O, et al.
    JAMA Pediatr, 2024 Nov 01;178(11):1199-1207.
    PMID: 39348138 DOI: 10.1001/jamapediatrics.2024.3330
    IMPORTANCE: The prevalence estimates of physical activity, sedentary behavior, and sleep (collectively known as movement behaviors) in 3- and 4-year-old children worldwide remains uncertain.

    OBJECTIVE: To report the proportion of 3- and 4-year-old children who met the World Health Organization guidelines for physical activity, sedentary behavior, and sleep across 33 countries.

    DESIGN, SETTING, AND PARTICIPANTS: Pooled analysis of data from 14 cross-sectional studies (July 2008 to September 2022) identified through systematic reviews and personal networks. Thirty-three countries of varying income levels across 6 geographical regions. Each study site needed to have at least 40 children aged 3.0 to 4.9 years with valid accelerometry and parent-/caregiver-reported screen time and sleep duration data. Data were analyzed from October 2022 to February 2023.

    EXPOSURES: Time spent in physical activity was assessed by reanalyzing accelerometry data using a harmonized data-processing protocol. Screen time and sleep duration were proxy reported by parents or caregivers.

    MAIN OUTCOMES AND MEASURES: The proportion of children who met the World Health Organization guidelines for physical activity (≥180 min/d of total physical activity and ≥60 min/d of moderate- to vigorous-intensity physical activity), screen time (≤1 h/d), and sleep duration (10-13 h/d) was estimated across countries and by World Bank income group and geographical region using meta-analysis.

    RESULTS: Of the 7017 children (mean [SD] age, 4.1 [0.5] years; 3585 [51.1%] boys and 3432 [48.9%] girls) in this pooled analysis, 14.3% (95% CI, 9.7-20.7) met the overall guidelines for physical activity, screen time, and sleep duration. There was no clear pattern according to income group: the proportion meeting the guidelines was 16.6% (95% CI, 10.4-25.3) in low- and lower-middle-income countries, 11.9% (95% CI, 5.9-22.5) in upper-middle-income countries, and 14.4% (95% CI, 9.6-21.1) in high-income countries. The region with the highest proportion meeting the guidelines was Africa (23.9%; 95% CI, 11.6-43.0), while the lowest proportion was in North and South America (7.7%; 95% CI, 3.6-15.8).

    CONCLUSIONS AND RELEVANCE: Most 3- and 4-year-old children in this pooled analysis did not meet the current World Health Organization guidelines for physical activity, sedentary behavior, and sleep. Priority must be given to understanding factors that influence these behaviors in this age group and to implementing contextually appropriate programs and policies proven to be effective in promoting healthy levels of movement behaviors.

    MeSH terms: Child, Preschool; Cross-Sectional Studies; Female; Humans; Male; Global Health; Exercise*; Sedentary Lifestyle*; Accelerometry
  7. Kolbasov GA, Savchenko AS
    Zootaxa, 2024 Dec 18;5555(2):182-200.
    PMID: 40173587 DOI: 10.11646/zootaxa.5555.2.2
    More than 30 specimens of both sexes of a new endoparasitic ascothoracidan, described herein as Sessilogoga perfuga sp. nov. in the family Synagogidae, were found infecting the colonies of the antipatharian Antipathes cf. curvata at 25-29 m depth in the vicinity of Bidong Island, Kuala Nerus District, Malaysia. Several individuals were observed alive and photographs and videos were taken of parasites both inside the host and after they have left the coral. Scanning electron microscopy (SEM) was used supplementing light microscopy examinations to study external morphology of both sexes at the ultrastructural level. Carapace ornamentation, armature of antennules, telson and furcal rami, as well as fine details of the mouthparts were studied with SEM. All three known species of the genus Sessilogoga share several synapomorphies, probably connected with their endoparasitic way of life, that distinguish them from closely related Synagoga and Waginella: (i) a smaller frontal filament complex; (ii) the absence of epaulets on thoracomere 6; (iii) fewer setae on the proximal antennular segments; and (iv) more seminal receptacles in any given pair of thoracopods. Here we discuss characters that can be used for identification of Sessilogoga species: (i) the armament of antennules; (ii) the form of the tips of maxillules; (iii) setation of thoracopods; (iv) number of seminal receptacles; (v) structure of telsonic spines; and (vi) length/height ratio of furcal rami. Molecular gene sequences of CO1 marker of the new species are provided for future phylogenetic analysis of the Ascothoracida.
  8. Loh KH, Poong SW, DU J, Ong JJL, Zheng X, Li Y, et al.
    Zootaxa, 2024 Dec 10;5550(1):133-144.
    PMID: 40173657 DOI: 10.11646/zootaxa.5550.1.14
    Groupers of the family Epinephelidae constitute a diverse and commercially valuable group of reef fishes globally. They comprise an assemblage of carnivorous marine fishes, comprising more than 177 species across 16 genera. The epinephelid genus Epinephelus, which consists of over 90 species, is found worldwide in the tropics and subtropics. To date, the ichthyofauna of Malaysia has documented a total of 43 epinephelid species. Apart from these, Epinephelus flavocaeruleus (Lacepède, 1802), commonly known as the blue-and-yellow grouper, is rarely reported in the Indo-Pacific Ocean. The present study extends the documented distribution range of E. flavocaeruleus eastwards from the Andaman Sea to the Borneo waters of Sabah, Malaysia. Five specimens of the blue-and-yellow grouper were collected from a local fish market. Species identification was confirmed by the color patterns and DNA barcoding of 630 base pairs of the cytochrome C oxidase I gene for all E. flavocaeruleus specimens, Epinephelus cyanopodus (Richardson, 1846), and 10 closely related Epinephelus species. The interspecies genetic distance ranged from 0.002-0.168. Results from the Templeton, Crandall, and Sing (TCS) haplotype network analysis and maximum likelihood phylogeny based on the COI marker indicate a close genetic relationship between E. flavocaeruleus and E. cyanopodus. However, we refrain from proposing any taxonomic revisions given that more in-depth studies using multiple molecular markers or phylogenomic analysis on a larger sample size are necessary to confirm the taxonomic status of both species. This study significantly contributes to a better understanding of the taxonomy, phylogenetic relationship, and genetic diversity of E. flavocaeruleus.
  9. Hanafi N, Chen MH, Chang CW, Chao NL, Seah YG
    Zootaxa, 2024 Dec 10;5550(1):85-117.
    PMID: 40173660 DOI: 10.11646/zootaxa.5550.1.11
    In terms of sheer abundance, taxonomic diversity, and species richness, Malaysia has one of the most diverse Sciaenidae in the Indo-West Pacific. Up to ten valid species of the sciaenid fish genus Johnius (e.g., J. amblycephalus (Bleeker, 1855), J. belangerii (Cuvier, 1830), J. borneensis (Bleeker, 1850), J. carouna (Cuvier, 1830), J. coitor (Hamilton, 1822), J. heterolepis Bleeker, 1873, J. macrorhynus (Lal Mohan, 1976), J. plagiostoma (Bleeker, 1849), J. sasakii Hanafi et al., 2022 and J. weberi Hardenberg, 1936), and with additional newly recorded species as J. trewavasae Sasaki, 1992, have been recorded to occur in East Malaysia (Borneo Island) coastal waters. The newly recorded species is a close congener to J. heterolepis Sasaki, 1992 and can be well distinguished by several characters and molecular evidence. The goal of this study is to review the taxonomic status of Johnius species in East Malaysia, Borneo Island, by using a literature review, morphological inference and molecular characters. Principal Component Analysis (PCA) successfully discriminates based on second anal spine length and gill raker length within sampled specimens. A phylogenetic tree based on cytochrome oxidase subunit I (COI) showed intraspecific levels of genetic differentiation within Johnius of East Malaysia, which forms a monophyletic group. Moreover, the new record of Johnius trewavasae in East Malaysia represented that the distribution of the subtropical genus Johnius extended to the southernmost part of the tropical region.
  10. Hasan MY, Roslan AHM, Azmi N, Ibrahim NM, Arulsamy A, Lee VLL, et al.
    J Mol Neurosci, 2024 Dec 24;75(1):2.
    PMID: 39718716 DOI: 10.1007/s12031-024-02300-9
    Elevated inflammatory reactions are a significant component in cerebral ischemia-reperfusion injury (CIRI). Activation of α7-Nicotinic Acetylcholine Receptor (α7nAChR) reduces stroke-induced inflammation in rats, but the anti-inflammatory pathway in microglia under CIRI condition remains unclear. This study employed qRT-PCR, protein assays, NanoString analysis, and bioinformatics to examine the effects of PNU282987 treatment (α7nAChR agonist) on BV2 microglial functional differentiation in oxygen-glucose deprivation/reoxygenation (OGDR) condition. OGDR significantly increased the gene expression of pro-inflammatory markers such as TNF-α, IL-6, and IL1β, while α7nAChR agonists reduced these markers. The anti-inflammatory gene marker IL-10 was upregulated by α7nAChR agonist treatment. Downstream pathway marker analysis showed that both gene and protein expression of NFκB was associated with anti-inflammatory effects. Blocking microRNA-21 with antagomir reversed the anti-inflammatory effects. NanoString analysis revealed that microRNA-21 inhibition significantly affected inflammation-related genes, including AL1RAP, TLR9, FLT1, PTGIR, NFκB, TREM2, TNF, SMAD7, FOS, CCL5, IFIT1, CFB, CXCL10, IFI44, DDIT3, IRF7, OASL1, IL1A, IFIT2, C3, CD40, STAT2, IFIT3, IL1RN, OAS1A, CSF1, CCL4, CCL2, CCL3, BCL2L1, and ITGB2. Enrichment analysis of upregulated genes identified Gene Ontology Biological Processes related to cytokine responses and TNF-associated pathways. This study highlights α7nAChR activation as a key regulator of anti-inflammatory responses in BV2 microglia under OGDR conditions, with micro-RNA21 identified as a crucial mediator of receptor-driven neuroprotection via the TNF-α/NFκB signalling pathway.
    MeSH terms: Animals; Benzamides/pharmacology; Bicyclo Compounds/pharmacology; Cell Line; Oxygen/metabolism; Signal Transduction; Cell Hypoxia; Nicotinic Agonists/pharmacology; Mice
  11. Mustafa NF, Cheng KK, Nadri MH, Razali SA, Zakaria II, Salin NH, et al.
    J Biomol Struct Dyn, 2024 Jun 17.
    PMID: 38881303 DOI: 10.1080/07391102.2024.2335296
    The rise in dengue cases in tropical and sub-tropical areas has become a significant health concern. At present, there is no definitive cure for dengue fever, which underscores the importance of identifying potent inhibitors. Dengue NS2B-NS3 protease is the prime drug target due to its vital function for replication. Quercetin, a flavone, has anti-dengue virus properties but is limited by low bioavailability. Previous studies have shown that methoxy substitution in flavones improves bioavailability and metabolic stability. Azaleatin is a derivative of quercetin with a methoxy substitution at the C5 position, however its ability to inhibit dengue is unknown. In this study, azaleatin was investigated for its inhibition against dengue NS2B-NS3 protease using in vitro and in silico techniques. The fluorescence assay was used to determine the IC50 value and inhibition kinetics. The molecular interaction between azaleatin and NS2B-NS3 was studied using CB-Dock2 and AutoDock Vina. The complex's stability was then analysed using GROMACS. Besides, the ADMETlab 2.0 was utilized to predict pharmacokinetic of the azaleatin. Results showed that azaleatin inhibits dengue NS2B-NS3 protease non-competitively with a Ki of 26.82 µg/ml and an IC50 of 38 µg/ml. Molecular docking indicated binding of the azaleatin to the allosteric pocket of NS2B-NS3 with a docking score of -8.2 kcal/mol. Azaleatin was found stable in the pocket along 100 ns, supporting its inhibitory mode. The compound has favourable pharmacokinetic profiles and conformed to Lipinski's Rule of Five. Taken together, azaleatin inhibits NS2B-NS3 protease in a non-competitive mode, suggesting its potential as safer anti-dengue compound.
  12. Aliyu AB, Jalila A, Saleha AA, Zunita Z
    Zoonoses Public Health, 2024 Dec;71(8):962-971.
    PMID: 39289890 DOI: 10.1111/zph.13179
    INTRODUCTION: Extended-spectrum β-lactamase-producing Escherichia coli (ESBL-EC) are widespread multidrug-resistant zoonotic bacteria that threaten animal production, food safety and antimicrobial therapy worldwide including Malaysia. Poultry has been reported as one of the pathways for human exposure to ESBL-EC. There has been little research on the occurrence of ESBL-EC within the Malaysian poultry food chain. Hence, the objectives of the study were to determine the occurrence of ESBL-EC in chickens and to identify the potential risk factors associated with their occurrence in poultry farms.

    METHODS: A cross-sectional study was conducted on 400 samples, consisting of 240 cloacal swabs from chickens and 160 from poultry farms environments in eight districts in Selangor, Malaysia using culture and disk combination methods and multiple polymerase chain reaction assays. In the determination of possible factors associated with the presence of ESBL-EC at poultry farms, a questionnaire was used to obtain the information and data.

    RESULTS: The findings demonstrated the wide distribution of ESBL-EC in all the farms with an overall occurrence of 37.2%. Farms in Gombak, Klang and Hulu Selangor had the highest occurrence rates at 62%, 50% and 50%, respectively, followed by farms in Petaling 38%, Sepang at 34%, Kuala Langat at 26% and Kuala Selangor at 24%, and the lowest was in Hulu Langat 14%. Among the study samples, chickens had the highest occurrence rate at 45.4%, followed by chicken house floors at 40% and flies at 30%, while feed and water samples at 17.5% and 12.5%, respectively. The present study indicated the high occurrence and wide dissemination of ESBL-EC in chickens and poultry farms environment. The ESBL-EC occurrence was associated with several factors including imprudent use of antibiotics, poor husbandry, management and biosecurity practices at the farms.

    CONCLUSIONS: Our study showed the presence and spread of ESBL EC among chickens in the farms and their environment; this may lead to being spread to outside of farm environment by flies, vermins, flying birds, litter and farm wastes and possibly to humans upon contact with the contaminated environment and by poultry meat. Thus, the findings of the study can assist to serve as a piece of useful information to veterinary authority in designing evidence-based mitigation strategies for the control of ESBL-EC in poultry farms.

    MeSH terms: Animals; Animal Husbandry; Cross-Sectional Studies; Humans; Malaysia/epidemiology; Public Health; Risk Factors; Farms
  13. Bala Ganesh KSVA, Panda P, H Makwana A, Gopalakrishna PK, Prameela Rani K, Vishnumukkala T
    Bioinformation, 2024;20(11):1588-1592.
    PMID: 40162442 DOI: 10.6026/9732063002001588
    The amygdala is a complex cellular structure situated centrally in the brain, adjacent to the hippocampus. It is an integral part of the limbic system and is essential for the processing of emotional reactions. The amygdala possesses extensive connections to multiple brain regions, enabling it to acquire sensory information and affect responses. The amygdaloid complex has over ten nuclei located in the mid-temporal lobe. It is quite likely that the primary root of certain anxiety disorders in humans, such as posttraumatic stress disorder, is a dysfunction in the processing of information associated with fear. This article analyzes the anatomical and physiological foundations suggested to support amygdala function.
  14. Jose AT, Kaur T, Sarangi S, Khan S, Tripathi S, Chandrasekaran S, et al.
    Bioinformation, 2024;20(11):1542-1548.
    PMID: 40162456 DOI: 10.6026/9732063002001452
    Denture marking is a vital tool in forensic odontology and clinical practice, aiding in the identification of dental prostheses, especially in disaster victim identification and cases where traditional methods fail. Techniques are categorized into surface methods like engraving and laser etching and inclusion methods such as embedding RFID tags or barcodes. These markers provide reliable identification, assist in recovering lost prostheses and can be implemented at low cost in routine dental practice. Advancements like RFID technology have made denture marking more efficient and reliable. Overall, denture marking enhances patient safety, reduces redundancy and holds significant medico-legal value.
  15. Ali N, Alaagib N, Abdelatif A, Ali O, Elmagbol W, Alhojaily S, et al.
    Niger J Physiol Sci, 2024 Dec 31;39(2):195-200.
    PMID: 40156787 DOI: 10.54548/njps.v39i2.5
    Diabetes problems are more likely to occur in people with low vitamin D levels. In this study, Sudanese individuals diagnosed with type 2 diabetes mellitus (T2DM) had their serum glucose, HbA1c, vitamin D, and inflammatory markers assessed. Thirty men and thirty women who had been diagnosed with T2DM for at least two years were among the 120 participants of both sexes, ages 35 to 69, who were enrolled in this case-control study. As a control group, the second group consisted of sixty healthy individuals (30 men and 30 women). Every participant had a thorough medical history taken, with special attention to the length of their diabetes, its medical history, and any previous problems. During the clinical examination and laboratory tests, the following criteria were evaluated: total WBC, ESR, CRP, HbA1c, and serum vitamin D3. When 25(OH) vitamin D3 levels were utilized to assess T2DM patients, they showed significantly lower mean blood concentrations than controls: 23 (38.3%) had vitamin D insufficiency, 23 (38.3%) had vitamin D deficiency, and 14 (23.3%) had sufficient vitamin D. Vitamin D-deficient patients had significant increases in HbA1c, glucose, CRP, and total WBC. Additionally, when comparing the mean ESR values of diabetes patients to those of the control group, there is a statistically significant rise. ESR did not significantly alter depending on the controlling level. Males also had a numerically higher level of vitamin D3 than females. Compared to healthy normal controls, individuals with type 2 diabetes have noticeably reduced vitamin D3 levels. Furthermore, in T2DM patients, there was a connection between elevated inflammatory markers and HbA1c and insufficiency in vitamin D.
    MeSH terms: Adult; Aged; C-Reactive Protein/analysis; C-Reactive Protein/metabolism; Female; Humans; Inflammation/blood; Inflammation/diagnosis; Male; Middle Aged; Case-Control Studies
  16. Abraham DA, Narasimhan U, Mahalingam VT, Krishnan M, Ganesan RM, Goh KW, et al.
    Front Biosci (Landmark Ed), 2024 Oct 23;29(10):365.
    PMID: 39473402 DOI: 10.31083/j.fbl2910365
    BACKGROUND: Literature indicates that L-carnosine may be deficient in autism spectrum disorder (ASD) children. The aim of the present study was to estimate the level of L-carnosine in plasma and correlate it with the Autism Treatment Evaluation Checklist (ATEC) and Childhood Autism Rating Scale 2nd Edition, Standard Version (CARS2-ST) scores. To measure L-carnosine level, a bio-analytical method was developed using reverse phase high- liquid chromatography and validated as per International Conference on Harmonization guidelines.

    METHOD: Children were supplemented with L-carnosine (10-15 mg/kg) along with standard care therapies for 2 months. Before and after supplementation, scores on the ATEC, CARS2-ST, BEARS sleep screening tool, 6-item Gastrointestinal Severity Index, and Parental Stress Scale were evaluated, and L-carnosine was measured at the end of the trial.

    RESULTS: The calibration curve was linear in the range of 100-600 ng/mL (R2 = 0.998). The level of L-carnosine quantified was 33.7 ± 0.2 ng/mL. There was no significant difference found in any of the outcome measures (p > 0.05).

    CONCLUSIONS: Despite the fact that L-carnosine is detectable in the blood, it was found to be ineffective in the management of ASD in children.

    CLINICAL TRIAL REGISTRATION: The study was registered in the Clinical Trial Registry-India, registration number: CTRI/2019/07/020102.

    MeSH terms: Child; Child, Preschool; Chromatography, High Pressure Liquid/methods; Female; Humans; Male; Pilot Projects; Dietary Supplements
  17. Park JM, Su YH, Fan CS, Chen HH, Qiu YK, Chen LL, et al.
    Exp Mol Med, 2024 Sep;56(9):2065-2081.
    PMID: 39294443 DOI: 10.1038/s12276-024-01300-4
    Ferritin, comprising heavy (FTH1) and light (FTL) chains, is the main iron storage protein, and pancreatic cancer patients exhibit elevated serum ferritin levels. Specifically, higher ferritin levels are correlated with poorer pancreatic ductal adenocarcinoma (PDAC) prognosis; however, the underlying mechanism and metabolic programming of ferritin involved in KRAS-mutant PDAC progression remain unclear. Here, we observed a direct correlation between FTH1 expression and cell viability and clonogenicity in KRAS-mutant PDAC cell lines as well as with in vivo tumor growth through the control of proline metabolism. Our investigation highlights the intricate relationship between FTH1 and pyrroline-5-carboxylate reductase 1 (PYCR1), a crucial mitochondrial enzyme facilitating the glutamate-to-proline conversion, underscoring its impact on proline metabolic imbalance in KRAS-mutant PDAC. This regulation is further reversed by miR-5000-3p, whose dysregulation results in the disruption of proline metabolism, thereby accentuating the progression of KRAS-mutant PDAC. Additionally, our study demonstrated that deferasirox, an oral iron chelator, significantly diminishes cell viability and tumor growth in KRAS-mutant PDAC by targeting FTH1-mediated pathways and altering the PYCR1/PRODH expression ratio. These findings underscore the novel role of FTH1 in proline metabolism and its potential as a target for PDAC therapy development.
    MeSH terms: Animals; Ferritins/metabolism; Humans; Mutation*; Oxidoreductases; Gene Expression Regulation, Neoplastic; Carcinoma, Pancreatic Ductal/genetics; Carcinoma, Pancreatic Ductal/metabolism; Carcinoma, Pancreatic Ductal/pathology; Cell Line, Tumor; Cell Proliferation; Mice
  18. Hakim ML, Islam MT, Alam T, Alenezi AM, Singh MSJ, Islam MS, et al.
    Sci Rep, 2024 Sep 27;14(1):22074.
    PMID: 39333182 DOI: 10.1038/s41598-024-72100-z
    A metamaterial-incorporated high-gain and broadband dipole antenna is proposed for 5G mm-wave applications. The designed high refractive index metamaterial (HRIM) properties are presented in detail with supporting results. The proposed dipole antenna achieved broadband features, and the antenna gain increased significantly by incorporating HRIM in the electromagnetic (EM) wave propagation path. Besides, the EM wave aggregation engineering of utilizing the HRIM on the dipole antenna is analyzed using the electric field, magnetic field, and power flow distributions. Both conventional and HRIM-based dipole antenna (HRIMDA) were fabricated on thin (0.254 mm) Rogers RT5880 substrate material with a low dielectric constant of 2.2, where the noticeable gain enhancement by HRIM is observed. The measured results show the operational bandwidth (BW) from 23 to 38 GHz frequency, and the highest gain of 9.5 dBi is accomplished at 35 GHz frequency. Finally, a four-element MIMO configuration is numerically and experimentally analyzed where the isolation of the two conjugated MIMO elements is  9.9 were also achieved. Hence, the proposed HRIM base dipole antenna is a potential candidate for 5G mm-wave applications.
  19. Salari N, Fattahi N, Abdolmaleki A, Heidarian P, Shohaimi S, Mohammadi M
    J Diabetes Metab Disord, 2024 Jun;23(1):395-403.
    PMID: 38932831 DOI: 10.1007/s40200-024-01408-4
    INTRODUCTION: fluctuation in serum levels of thyroid hormones or thyroid-associated dysfunction can negatively affect the sexual behaviors or performance, and disruption in couples` relationship or satisfaction. Thus, this systematic review and meta-analysis study was aimed to investigate the global prevalence of sexual dysfunction in men with thyroid gland disorders (TGD).

    METHODS: this systematic review and meta-analysis study conducted based on PRISMA statement criteria regarding the available evidences representing the prevalence of sexual dysfunction in men with TGD. The initial searching process was applied on July, 2023. In this era, the main keywords of "Prevalence", "Sexual disorders", "Sexual disorder", "Sexual dysfunction", "Male sexual dysfunction", "Erectile dysfunction", "Males", "Men", "Thyroid disorders", "Thyroid diseases", "Hyperthyroidism", "Thyroid", and "Thyroiditis" were hired. Also, "AND" and "OR" operators were used for keywords combination. All intended studies were searched using the databases of Web of Science, Google Scholar, Scopus, ScienceDirect, PubMed, and Embase. Random effects model was used to perform the analysis and the heterogeneity of the studies was assessed through I2 index. Data analysis was applied with CMA software (v.2).

    RESULTS: following the assessment of 17 eligible studies with a sample size of 501 individuals, the global prevalence of male sexual dysfunction with TGD was found 51.5% (95% CI:38.7-64). Also, the prevalence of male sexual dysfunction in hypothyroidism and hyperthyroidism cases was 59.1% (95% CI:37.2-77.8) and 41.5% (95% CI:25.9-59.1), respectively. The meta-regression analysis showed that following incremental trend in sample size, the global prevalence of male sexual dysfunction with TGD decreases. This assessment also revealed that the prevalence of male sexual dysfunction increases with the year of study conduction, significantly (p 

  20. Gauen AM, Wang Y, Perak AM, Davis MM, Rosenman M, Lloyd-Jones DM, et al.
    J Am Heart Assoc, 2024 Nov 05;13(21):e033589.
    PMID: 39450715 DOI: 10.1161/JAHA.123.033589
    BACKGROUND: Preventive screenings in children encourage maintenance of optimal cardiovascular health, but gaps may exist between recommendations and clinical practice. We evaluated adherence to pediatric guidelines for universal age-based and risk-based screening for body mass index, blood pressure, lipids, and blood glucose.

    METHODS AND RESULTS: We used 2010 to 2018 ambulatory visit data from children aged 2 to 12 years within CAPRICORN (Chicago Area Patient-Centered Outcomes Research Network), an electronic health record network in Chicago. This study included 87 549 children who attended 197 559 well-child encounters. Across all encounters, children were 51.5% male and mean (SD) age 6.4 (3.3) years. For each child who attended a well-child visit and met age and/or risk-based criteria, receipt of body mass index, blood pressure, lipids, and/or hemoglobin A1c or fasting blood glucose measurements were assessed. We used generalized estimating equations to calculate proportion adherence for each metric overall and stratified by age, sex, race and ethnicity, and insurance status. Universal age-based screening prevalence (95% CI) per 100 eligible visits was 77.1 (76.8-77.3) for body mass index, 33.4 (33.1-33.7) for blood pressure, and 9.6 (9.3-9.9) for lipids. Risk-based screening prevalence (95% CI) per 100 eligible visits was 13.9 (12.2-15.9) for blood pressure, 6.9 (6.4-7.5) for lipids, and 13.3 (12.6-14.1) for blood glucose.

    CONCLUSIONS: Early screening of cardiovascular health risk factors could lead to earlier interventions, which could alter cardiovascular health trajectories across the lifetime. Low-to-moderate levels of adherence to universal age-based and risk-based cardiovascular health screening highlight the gap between recommendations and clinical practice, emphasizing the need to understand and address barriers to screening in pediatric populations.

    MeSH terms: Age Factors; Blood Pressure; Chicago/epidemiology; Child; Child, Preschool; Female; Hemoglobin A, Glycosylated/analysis; Hemoglobin A, Glycosylated/metabolism; Humans; Lipids/blood; Male; Risk Factors; Body Mass Index*; Practice Guidelines as Topic; Risk Assessment
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