Browse publications by year: 2024

  1. Utomo B, Fatmaningrum W, Sulistiawati, Fauziyah S, Sucipto TH, Adnyana IMDM, et al.
    Helminthologia, 2024 Dec;61(4):293-299.
    PMID: 39989491 DOI: 10.2478/helm-2024-0040
    This study aims to investigate the soil-transmitted helminth infection among school-age children in Gresik, East Java, Indonesia. An analytical observational study with a cross-sectional design was conducted from July to November 2023 in selected elementary schools in Gresik, East Java, Indonesia. Logistic regression analysis was employed to assess the impact of each risk factor on the likelihood of infection transmission. A total of 183 school-age children were screened for the occurrence of helminth eggs in their feces. Twenty-two feces samples from 183 children were positive for helminth egg, consisting of 14 children infected by Ascaris lumbricoides (6.36 %), 3 children infected by Trichuris trichiura (1.304 %), 3 children infected by Oxyuris vermicularis (1.304 %), and 3 children infected by Hookworm (1.304 %). The highest prevalence was found in 7-year-old children (31.8 %). Children who pulled off shoes while in school were more likely to be infected with an overall OR of 8.392 (95 % CI 1.63 - 43.65). Children who played in the dumpsite were more likely to be infected with an overall OR of 4.521 (95 % CI 0.578 - 31.28). Children who washed before eating were more likely to be infected with an overall OR of 3.579 (95 % CI 0.587 - 21.835). Therefore, to lessen the prevalence of STHs among school-age children in Gresik, we recommend improving hygiene conditions in schools. The government should prioritize enrolling all primary schools in hygiene classes as the school health program.
  2. Panchal P, Usman M, Longkumer T, Babu RS, Khatib MN, Razak SA, et al.
    Front Nutr, 2024;11:1480319.
    PMID: 39995446 DOI: 10.3389/fnut.2024.1480319
    BACKGROUND: Children living in refugee camps in South Asian countries suffer from undernutrition. However, the emerging prevalence of double burden of malnutrition could potentially cause a crisis in the healthcare of the refugee population. Double burden increases the risk for co-morbidities, poor functional health, and increased risk for premature death among these children. The study aims to assess the prevalence of malnutrition among refugee children in South Asia.

    METHODS: This systematic review and meta-analysis followed the standard Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) 2020 guidelines using CoCoPop mnemonic approach. We searched JSTOR, Scopus, PubMed, Web of Science, and MEDLINE databases for studies on the prevalence of malnutrition in refugee children from 1984 to August 2024 with restricted English language. The screening of research articles was undertaken using COVIDENCE 2.0 software. The JBI checklist was used to assess the methodological quality of the included articles. The meta analysis was carried out using MedCalc 22.018 software. The gray literature was manually searched from the reputed organizations focusing on refugee children and was narratively analyzed for malnutrition statistics. Furthermore, the corroboration of primary research articles and gray literature was conducted for comprehensive understanding.

    RESULTS: The review included 10 full-text research articles, all with cross-sectional study design and 11 gray literatures. The 10 studies covered a total of 4,274 participants with 3,536 urban and 738 rural refugee children aged between 0 and 19 years [refugee children (n = 8) and refugee adolescents (n = 2)]. The sample size of the included studies varied between 58 and 1,087 and mostly from Bangladesh. The prevalence of stunting ranged from 3.9-75.4% in the included studies [pooled prevalence: 31.8% (95% CI: 18.6-46.6%)]; wasting between 0.3-24.3% [pooled prevalence:10.1% (95% CI: 4.6-17.3)]; underweight between 4.4-65% [pooled prevalence: 19.1% (95% CI: 10.8-29.2%)]; and overweight between 3 and 24% [pooled prevalence: 6.5% (95% CI: 2.6-12.1%)]. Time trend analysis of the prevalence of malnutrition showed a decreasing trend in underweight, an increasing trend for stunting and wasting, and overweight including a rising prevalence of dual burden of under-and overnutrition.

    DISCUSSION: The study indicates a high prevalence of undernutrition and a rising prevalence of overnutrition -the Asian paradox of the double burden of malnutrition in refugee children living in South Asia. The coexisting double burden of malnutrition among refugee children calls for comprehensive programs and policies for the prevention and management of the double burden of malnutrition.

  3. Gauhar V, Traxer O, Castellani D, Sietz C, Chew BH, Fong KY, et al.
    Eur Urol Focus, 2024 Dec;10(6):975-982.
    PMID: 38789313 DOI: 10.1016/j.euf.2024.05.010
    BACKGROUND AND OBJECTIVE: The aim of this study was to evaluate the stone-free status (SFS) rate and complications after flexible ureteroscopy (fURS) for treatment of renal stones using a flexible and navigable suction (FANS) ureteral access sheath.

    METHODS: Data for adults undergoing fURS in 25 centers worldwide were prospectively collected (August 2023 to January 2024). Exclusion criteria were abnormal renal anatomy and ureteral stones. All patients had computed tomography scans before and within 30 d after fURS with a FANS ureteral access sheath. SFS was defined as follows: grade A, zero fragments; grade B, a single fragment ≤2 mm; grade C, a single fragment 2.1-4 mm; and grade D, single/multiple fragments >4 mm. Data for continuous variables are presented as the median and interquartile range (IQR). Multivariable logistic regression was performed to evaluate predictors of grade A SFS.

    KEY FINDINGS AND LIMITATIONS: The study enrolled 394 patients (59.1% male) with a median age of 49 yr (IQR 36-61). The median stone volume was 1260 mm3 (IQR 706-1800). Thulium fiber laser (TFL) was used in 45.9% of cases and holmium laser in the rest. The median lasing time was 18 min (IQR 11-28) and the median operative time was 49 min (IQR 37-70). One patient required a blood transfusion and 3.3% of patients had low-grade fever. No patient developed sepsis. Low-grade ureteral injury occurred in eight patients (2%). The grade A SFS rate was 57.4% and the grade A + B SFS rate was 97.2%, while 2.8% of patients had grade C or D SFS. Eleven patients underwent repeat fURS. Multivariable analysis revealed that a stone volume of 1501-3000 mm3 (odds ratio 0.50) and of >3000 mm3 (odds ratio 0.29) were significantly associated with lower probability of grade A SFS, while TFL use was associated with higher SFS probability (odds ratio 1.83). Limitations include the lack of a comparative group.

    CONCLUSIONS AND CLINICAL IMPLICATIONS: fURS using a FANS ureteral access sheath resulted in a high SFS rate with negligible serious adverse event and reintervention rates.

    PATIENT SUMMARY: We looked at 30-day results for patients undergoing telescopic laser treatment for kidney stones using a special type of vacuum-assisted sheath to remove stone fragments. We found a high stone-free rate with minimal complications.

    MeSH terms: Adult; Equipment Design; Europe; Female; Humans; Male; Middle Aged; Postoperative Complications/etiology; Postoperative Complications/epidemiology; Prospective Studies; Suction; Time Factors; Ureter/surgery; Treatment Outcome; Ureteroscopes
  4. Lim YS, Aim N, Shahar S
    BMJ Open, 2024 Oct 02;14(10):e081226.
    PMID: 39357982 DOI: 10.1136/bmjopen-2023-081226
    INTRODUCTION: The presence of food product indicators may facilitate consumers in making informed healthy choices. However, it may also mislead consumers. This study aims to determine the prevalence of food products carrying food product indicators; the compliance of products bearing Malaysia's Healthier Choice Logo (HCL) and nutrition and health claims (NHC) towards the local regulations; and the credibility of the aforementioned products as healthy food products based on the international regulation in the Malaysian market.

    METHOD: This is a cross-sectional market survey conducted from February to May 2023 on 3428 products sold in Malaysian supermarkets. Product information including the brand, name, nutrition information panel, food product indicator (front-of-pack nutrition labelling, NHC, other claims), ingredients list and manufacturer or importer were collected. Compliance of products carrying NHC and HCL is evaluated against local guidelines. Credibility as a healthy product is evaluated against the WHO Nutrient Profile Model for the Western Pacific Region on a subsample (products with HCL and/or NHC).

    RESULTS: 53% of food products surveyed had food product indicators (n=1809). A total of 32% carried at least one NHC (n=1101), of which 47% had excellent overall compliance (n=522). Only 4% carried Malaysia's HCL (n=138), of which 48% had excellent nutrient compliance (n=66). Only 13% of the products carrying Malaysia's HCL and NHC could be identified as absolute healthy food products as defined by the WHO standard (n=147).

    CONCLUSION: Although half of the products surveyed had food product indicators, merely half of them had excellent compliance towards the standards. Only 13% of the subsample qualified as healthy food products. Voluntary application of the local HCL was low among food industries. Ensuring high standards of compliance and credibility of food products in the Malaysian market is crucial for food companies and government authorities.

    MeSH terms: Consumer Behavior/statistics & numerical data; Cross-Sectional Studies; Humans; Malaysia; Nutritive Value; Surveys and Questionnaires; Healthy Diet/standards; Healthy Diet/statistics & numerical data
  5. Hussain B, Khalily MT, Waqas A, Rahman A, Angelakis I, Nisar A, et al.
    Front Psychiatry, 2024;15:1413809.
    PMID: 39980593 DOI: 10.3389/fpsyt.2024.1413809
    BACKGROUND: In Pakistan, the mental health care for persons living with disabilities is insufficient, lacking a holistic approach to address their needs. This research evaluates the adapted PM+'s (IA-PM+) initial efficacy and acceptability in improving mental health among persons living with disabilities, contributing to the integration of mental health services within primary care settings in Pakistan.

    METHODS: This study employed a two-arm, single-blind cluster randomized controlled trial in a community setting within Union Council Kuri Dolal, Tehsil Gujjar Khan, District Rawalpindi-Pakistan. It compared an Active Treatment group, receiving five sessions of the IA-PM+ intervention, against a Delayed Treatment Control group. A total of 148 participants were randomized. Randomization occurred at the village level, with clusters assigned to either the intervention or control group. The intervention's effectiveness and participant satisfaction were assessed using a variety of measures, including the GHQ-12, WHODAS 2.0, DASS-21, and the Client Satisfaction Questionnaire, at baseline, the 8th week, and during a 3-month follow-up. Qualitative feedback was gathered at the end of the study to assess the intervention's acceptability among the participants.

    RESULTS: The study achieved a 100% retention rate. Baseline demographics showed a majority male participation with a variety of disabilities predominantly lower limb. Significant improvements were noted in the intervention group across WHODAS scores (Cohen's d= 0.66), PTSD symptoms (d= 0.75), and DASS scores for depression (d= 0.46), anxiety (d=0.65), and stress (d= 0.47). Similar trends were noted during the follow-up phases. However, life satisfaction scores initially higher in the control group evened out by follow-up, and perceived social support was consistently lower in the intervention group. High levels of client satisfaction were reported in the intervention group, with most participants finding the sessions beneficial and expressing willingness to recommend the service to others. The qualitative interviews revealed that the integration of religious practices, such as associating stress management techniques with Adhan, significantly enhanced the acceptability of the culturally adapted PM+ intervention. Participants reported that this cultural alignment made the coping strategies more relatable and easier to adopt, contributing to improvements in both their mental health and somatic symptoms.

    CLINICAL TRIAL REGISTRATION: https://clinicaltrials.gov/ct2/show/NCT04981522, identifier NCT04981522.

  6. Zainal N, Azizan A
    Ethiop J Health Sci, 2024 Jul;34(4):331-344.
    PMID: 39981017 DOI: 10.4314/ejhs.v34i4.9
    BACKGROUND: Global population aging has sparked research into frailty and falls given their impact on older adults. This study provides a bibliometric analysis of frailty and fall literature to identify publication trends, leading contributors, impactful works, and conceptual themes.

    METHODS: Frailty and fall publications were retrieved from Scopus and Web of Science databases without date restrictions. Data was analyzed using ScientoPy, and VOSviewer to generate statistics, visualizations, and maps based on temporality, productive countries, institutions, citations, subject categories, and keyword occurrences.

    RESULTS: After pre-processing, 345 publications remained (84.6% Web of Science, 15.4% Scopus). The literature has grown steadily since 1990, led by the United States, China, and Japan. Prolific institutions were identified, including Pittsburgh University. Highly cited impactful studies were published across journals like the Journal of the American Geriatrics Society. Geriatrics/gerontology was the dominant subject category. Keyword co-occurrence analysis revealed clusters focusing on geriatric physical health, cardiovascular health, cognition, interventions, and mortality.

    CONCLUSIONS: This bibliometric analysis synthesizes a comprehensive overview of frailty and fall research, identifying rising publication and citation trends, leading global contributors, impactful studies, and thematic focuses. The findings can inform resource allocation, international collaboration, impactful evidence utilization, and future research planning to advance frailty science and clinical care for older populations. Ongoing investigation is warranted into frailty mechanisms, assessment, management, and multidomain interventions.

    MeSH terms: Aged; Geriatrics/methods; Humans; Bibliometrics*; Frail Elderly/statistics & numerical data
  7. Rohsiswatmo R, Dewi R, Sutantio J, Amin Z, Youn YA, Kim SY, et al.
    Front Pediatr, 2024;12:1517843.
    PMID: 39981407 DOI: 10.3389/fped.2024.1517843
    BACKGROUND: Optimum neonatal resuscitation practices are vital for improving neonatal survival and neurodevelopment outcomes, particularly in extremely preterm infants. However, such practices may vary between high-income countries (HICs) and low-middle-income countries (LMICs). This study aimed to evaluate the resuscitation practices of high-risk infants in a large multi-country sample of healthcare facilities among HICs and LMICs in Asia under the AsianNeo Network.

    METHODS: In 2021, a customized 6-item online survey on resuscitation practices of infants born at <29 weeks gestation (or birth weight <1,200 g) was sent by the representative of each country's neonatal network to all the Neonatal Intensive Care Units (NICUs) participating in AsianNeo network. At the time of the survey, there were 446 participating hospitals in eight countries: four high-income countries (Japan, Singapore, South Korea, and Taiwan) and four low-middle-income countries (Malaysia, Indonesia, Philippines, and Thailand).

    RESULTS: The study included 446 hospitals, with a response rate of 72.6% (ranging from 62.7% to 100%), with 179 (55.2%) in HICs and 145 (44.7%) in LMICs. Routine attendance of experienced NICU physicians during resuscitations is reported to be higher in HICs than LMICs, both during daytime (79% vs. 40%) and nighttime (62% vs. 23%). The NRP guidelines in each country were varied, with 4 out of 8 countries using indigenously developed guidelines. Equipment availability during resuscitation was also variable; saturation monitors, radiant warmers, and plastic wraps were available in almost all hospitals, whereas oxygen and air blenders, heated humidified gas, and end-tidal CO2 detectors were more available in HICs. The most common device for Positive Pressure Ventilation (PPV) was the T-piece resuscitator (52.3%).

    CONCLUSION: The neonatal resuscitation practices for extremely preterm infants, encompassing staff, equipment, and guidelines, exhibited variance between HICs and LMICs in the AsianNeo region. Further enhancements are imperative to narrow this gap and optimize neonatal outcomes.

  8. Javaid MU, Farooq S, Arshad MZ, Ibikunle AK, Ibrahim AM
    Work, 2024 Dec 10.
    PMID: 39973692 DOI: 10.1177/10519815241290108
    BACKGROUND: At a global scale, the impact of the manufacturing sector on the environment has led to the increased demand for the sustainable practices and strategies that help the businesses achieve environmental, social, and economic objectives particularly within the context of developing economies.

    OBJECTIVE: This paper addresses this gap by investigating the relationship between employees and firms' environmental performance in the manufacturing sector operating in a developing country, Pakistan-where the environmental focus is sparse and organizational structures rarely follow cross-functional systems.

    METHODS: Quantitative research was employed and SmartPLS technique was used to test the theoretical model with a valid response rate of 77 percent of senior and middle-level managers of manufacturing firms.

    RESULTS: Results revealed the significance of green HRM as direct effect with all constructs. And internal environment management mediates the relationship (β = 0.158; t--value = 3.458; p 

  9. Ijod G, Nawawi NIM, Sulaiman R, Ismail-Fitry MR, Adzahan NM, Anwar F, et al.
    Food Chem X, 2024 Dec 30;24:101882.
    PMID: 39974709 DOI: 10.1016/j.fochx.2024.101882
    Mangosteen pericarp (MP) is abundant in bioactive compounds but is often discarded as waste, leading to environmental pollution. This study compared the extraction of dried MP using maceration and high-pressure processing (HPP). HPP at 10 min (500 MPa/20 °C) resulted in the highest ACNs, total phenolic content (TPC), total flavonoid content (TFC), and antioxidant activities. It also significantly reduced residual enzyme activities (REA) of polyphenol oxidase (PPO) and peroxidase (POD) by 33.90 % and 8.27 %, respectively. Fourier-transform infrared spectroscopy (FT-IR) analysis revealed a new wavelength at 2665.25 cm-1, and scanning electron microscopy (SEM) showed significant pore formation in MP cells, indicating cell damage. HPP-10 min enhanced the extraction of bioactive compounds, which significantly elevated the thermal stability of ACNs at 60 °C. This suggests that HPP is a promising method for extracting and preserving ACNs and other bioactive compounds from dried MP, with potential applications as natural colorants.
  10. Amakhmakh M, Hajib A, Belmaghraoui W, Harhar H, Mohammed EA, Al Abdulmonem W, et al.
    Food Chem X, 2024 Dec 30;24:101875.
    PMID: 39974711 DOI: 10.1016/j.fochx.2024.101875
    The pomegranate, Punica granatum L. (Punicaceae), stands as one of the most widely employed oils in the cosmetic industry. However, due to its higher content of conjugated linoleic acid, its susceptibility to oxidation is a major challenge, with the most prominent being punicic acid. This study aimed to evaluate the effects of traditional roasting in a microwave on the lipid content, nutritional value, and oxidative stability of Moroccan pomegranate seed oil. The findings indicated a rise in the amount of oil after 15 min of roasting at 650 W, the amount of oil rose from 27.03 to 30.10 (g/100 g). However, the protein content, UV absorbance values, iodine, and saponification values were not significantly affected by a longer roasting time. The peroxide value increases with roasting (1.00 to 5.00 M.eq. O2/kg oil). The roasting process under 350 W did not significantly alter the fatty acid composition. The total tocopherol content exhibits a decrease with increasing roasting time and power, ranging from 333.36 mg/100 g for unroasted seeds to 316.84 mg/100 g for seeds roasted under the conditions of 650 W for 15 min. The roasting process has proven to be critical for the immediate and long-term preservation of the nutritional and physico-chemical properties of pomegranate seed oil.
  11. Manyong V, Nguezet PMD, Nyamuhirwa DA, Osabohien R, Bokanga M, Mignouna J, et al.
    Heliyon, 2024 Nov 15;10(21):e40207.
    PMID: 39975457 DOI: 10.1016/j.heliyon.2024.e40207
    Access to adequate and nutritious food is accepted as a human right worldwide. In the Democratic Republic of Congo (DRC), Kasai Oriental province is one of the most vulnerable provinces in the country in terms of food insecurity. However, its current depth of food insecurity and the root factors have not been studied. Against this background, this study used cross-sectional data from 318 households to analyze the magnitude and socioeconomic drivers of food insecurity among rural households in the province. We developed two food security indicators: the food consumption score (FCS) and household food insecurity access scale (HFIAS), and applied the ordinary least square (OLS) and the negative binomial model for the analysis. Our findings show that households rely mainly on vegetables to meet their food need, consume more energy, and have limited access to protein, vitamin, and fat-rich foods. All the surveyed households were deficient in food quantity, while 75 % were deficient in quality. The study noted that the severity of food insecurity is zone-specific and more pronounced in the Kabeya Kamwanga territory than in others. Importantly, the poverty and education levels among households associated with large household sizes were the significant determinants of food insecurity in the area. These results strongly demonstrate the need for agrifood interventions that foster education, enable efficient land use, and target poor households in the province.
  12. Kowal M, Bode A, Koszałkowska K, Roberts SC, Gjoneska B, Frederick D, et al.
    Hum Nat, 2024 Dec;35(4):430-450.
    PMID: 39725786 DOI: 10.1007/s12110-024-09482-6
    Given the ubiquitous nature of love, numerous theories have been proposed to explain its existence. One such theory refers to love as a commitment device, suggesting that romantic love evolved to foster commitment between partners and enhance their reproductive success. In the present study, we investigated this hypothesis using a large-scale sample of 86,310 individual responses collected across 90 countries. If romantic love is universally perceived as a force that fosters commitment between long-term partners, we expected that individuals likely to suffer greater losses from the termination of their relationships-including people of lower socioeconomic status, those with many children, and women-would place a higher value on romantic love compared to people with higher status, those with fewer children, and men. These predictions were supported. Additionally, we observed that individuals from countries with a higher (vs. lower) Human Development Index placed a greater level of importance on romantic love, suggesting that modernization might influence how romantic love is evaluated. On average, participants worldwide were unwilling to commit to a long-term romantic relationship without love, highlighting romantic love's universal importance.
    MeSH terms: Adult; Cross-Cultural Comparison*; Female; Humans; Interpersonal Relations; Love*; Male; Middle Aged; Young Adult
  13. Jafarzadeh S, Qazanfarzadeh Z, Majzoobi M, Sheiband S, Oladzadabbasabad N, Esmaeili Y, et al.
    Curr Res Food Sci, 2024;9:100882.
    PMID: 39958969 DOI: 10.1016/j.crfs.2024.100882
    With a growing global population and the resulting pressure on natural resources, the supply of high-value protein has become increasingly limited. The rise of environmental and ethical concerns has led to the emergence of meat analogues as a credible alternative to traditional animal-derived meat. Growing demand for plant-based protein sources has gained attention as viable alternatives to conventional animal proteins. This article reviews commercially available plant proteins for meat replacement and evaluates recent research on producing meat analogues, highlighting their advantages and limitations. Beyond production, an examination of the physicochemical, textural, and structural attributes of the meat alternatives is conducted, highlighting the improvements made in achieving sensory and nutritional parallels with animal-derived meat. Furthermore, this article explores the current commercial applications of meat alternatives, highlighting the challenges faced in their widespread adoption and suggesting future research directions. The comparison of the environmental impacts of plant proteins and animal proteins is also presented. The ultimate goal is to develop meat substitutes that closely mimic the sensory, nutritional, and aesthetic qualities of real meat. Despite promising innovations in processing technologies, challenges remain that researchers are actively addressing to close the gap between plant-based meat analogues and animal-derived counterparts.
  14. Wang J, Balakrishnan B, Wan X, Yu Q, Ye Q
    Front Psychol, 2024;15:1485265.
    PMID: 39949975 DOI: 10.3389/fpsyg.2024.1485265
    INTRODUCTION: Over the past decade, there has been a growing focus on the study of how religion and technology intersect, particularly within the field of digital religion studies. In recent years, digital religious activities have emerged on Chinese social media platforms, with the sharing and promotion of content related to religious activities becoming increasingly popular. In a country like China, which adheres to atheistic ideology, the emergence of religious activities among the Generation Z youth provides a unique case for academic research. However, there is very limited research on digital religious behavior in mainland China. This study fills this gap by extending the Theory of Planned Behavior (TPB) to predict additional influencing factors of digital religious intentions and behavior.

    METHODS: This study employed a quantitative design, disseminating surveys via Sina Weibo and the Douyin platform. We collected a total of 525 valid responses. This study aims to deeply explore the social and psychological factors generated by digital religious activities on social media platforms, particularly how they stimulate Chinese Generation Z youth to participate in digital religious activities.

    RESULTS: The results show that attitudes toward digital religion, perceived behavioral control, social identity, and empathetic willingness are predictors of intentions, while social norms are not. Intentions significantly predict users'behavior on social media platforms such as Weibo and Douyin. Moreover, empathetic willingness and social identity fully mediate the effects of subjective norms, perceived behavioral control, and attitudes on intentions. Consequently, these behaviors generate impact, indicating the presence of multiple mediation effects.

    DISCUSSION: Unlike the societal context of theistic nations, there is a pronounced atheistic inclination within Chinese society. Consequently, subjective norms do not influence the digital religious behaviors of Chinese youth. Among contemporary Chinese youth, participation in digital religious practices is a temporary, secular activity undertaken only as a comforting behavior when anxiety becomes unmanageable or surpasses their threshold of tolerance. In contemporary China, young people face immense pressures stemming from workplace competition, social interactions, and economic burdens. Social media offers these young individuals broader opportunities for connection, community formation, and identity construction, as well as various possibilities for organizing their social lives. Consequently, on one hand, they turn to online religious avenues, seeking understanding from others through shared experiences, thereby obtaining emotional solace and comfort. On the other hand, they look to spiritual beliefs to alleviate anxiety, resolve confusion, and gain psychological comfort through emotional exchanges. Therefore, digital religion can, to some extent, be viewed as a form of social-technological empowerment, providing contemporary youth with a new "pressure valve" to facilitate emotional relief, comfort, and tension alleviation.

  15. Zhang WH, Gao JW, Lau CC, Jiang ZF, Yeong YS, Mok WJ, et al.
    World J Microbiol Biotechnol, 2024 Sep 19;40(10):325.
    PMID: 39294488 DOI: 10.1007/s11274-024-04130-8
    Euglena gracilis is a unique microalga that lacks a cell wall and is able to grow under different trophic culture conditions. In this study, cell growth, biomass production, and changes in the ultrastructure of E. gracilis cells cultivated photoautotrophically, mixotrophically, and under sequential-heterotrophy-photoinduction (SHP) were assessed. Mixotrophy induced the highest cell growth and biomass productivity (6.27 ± 0.59 mg/L/d) in E. gracilis, while the highest content of fatty acids, 2.69 ± 0.04% of dry cell weight (DCW) and amino acids, 38.16 ± 0.08% of DCW was obtained under SHP condition. E. gracilis also accumulated significantly higher saturated fatty acids and lower unsaturated fatty acids when cultivated under SHP condition. Transcriptomic analysis showed that the expression of photosynthetic genes (PsbA, PsbC, F-type ATPase alpha and beta) was lower, carbohydrate and protein synthetic genes (glnA, alg14 and fba) were expressed higher in SHP-culture cells when compared to other groups. Different trophic conditions also induced changes in the cell ultrastructure, where paramylon and starch granules were more abundant in SHP-cultured cells. The findings generated in this study illustrated that aerobic SHP cultivation of E. gracilis possesses great potential in human and animal feed applications.
    MeSH terms: Gene Expression Regulation, Developmental; Biomass; Gene Expression Profiling*; Heterotrophic Processes; Gene Ontology
  16. Kumari VBC, Ramu R, Huligere SS, Patil SM, Nayakvadi S, Bijoor S, et al.
    Front Microbiol, 2024;15:1502751.
    PMID: 39943964 DOI: 10.3389/fmicb.2024.1502751
    The gut microbiota plays a significant role in metabolic disorders such as diabetes and obesity, with the peroxisome proliferator-activated receptor gamma (PPAR-γ) being a key regulator in adipogenesis and glucose metabolism. This study is a novel approach that explores the antihyperglycemic and antihyperlipidemic effects of Levilactobacillus brevis RAMULAB54 (LB13243), isolated from fermented sugarcane juice. LB13243 was cultured for SEM imaging, and its supernatant (LBR54) was analyzed. Organic acid interactions with PPAR-γ were evaluated via molecular docking, while cytotoxicity and adipocyte differentiation in 3T3-L1 cells were tested using MTT assays, Oil Red O staining, triglyceride quantification, and qRT-PCR. In vivo, male Wistar rats in hyperlipidemic and streptozotocin-induced hyperglycemic models were treated with LB13243 for 4 weeks, followed by analysis of food intake, body weight, serum glucose, lipids, and histopathology. LB13243 inhibited carbohydrate-hydrolyzing enzymes and showed an organic acid profile. In silico, hydroxycitric acid had similar binding to PPARγ as rosiglitazone (binding energy:-8.4 kcal/mol vs.-8.3 kcal/mol), with greater stability (RMSD: 1.2 Å vs. 1.7 Å). Pharmacokinetics indicated moderate GI absorption (20%) and low toxicity for hydroxycitric acid. LBR54 did not affect 3T3-L1 cell viability but reduced lipid accumulation by 13% and triglycerides by ≤ 44 mg/dL. qRT-PCR revealed upregulation of PPAR-γ and C/EBP-α, and downregulation of FAS, suggesting modulation of adipogenesis. In vivo, LB13243 reduced food intake, weight gain, and normalized organ weights in hyperlipidemic rats, while improving glucose levels and lipid profiles in hyperglycemic models. Histopathology showed improved tissue structure, indicating LB13243's potential to reduce hyperglycemia and hyperlipidemia by modulating lipid metabolism and inflammation. LB13243's modulation of PPAR-γ suggests it as a promising natural option for managing diabetes and hyperlipidemia. This study also highlights LB13243's distinctive capability to modulate PPAR-γ through its organic acids, particularly hydroxycitric acid, providing novel insights into its therapeutic potential.
  17. Hayrapetyan A, Tumasyan A, Adam W, Andrejkovic JW, Bergauer T, Chatterjee S, et al.
    Phys Rev Lett, 2024 Oct 18;133(16):161805.
    PMID: 39485967 DOI: 10.1103/PhysRevLett.133.161805
    The first search for the Z boson decay to ττμμ at the CERN LHC is presented, based on data collected by the CMS experiment at the LHC in proton-proton collisions at a center-of-mass energy of 13 TeV and corresponding to an integrated luminosity of 138  fb^{-1}. The data are compatible with the predicted background. For the first time, an upper limit at the 95% confidence level of 6.9 times the standard model expectation is placed on the ratio of the Z→ττμμ to Z→4μ branching fractions. Limits are also placed on the six flavor-conserving four-lepton effective-field-theory operators involving two muons and two tau leptons, for the first time testing all such operators.
  18. Tomkinson GR, Lang JJ, Rubín L, McGrath R, Gower B, Boyle T, et al.
    J Sport Health Sci, 2024 Dec 06;14:101014.
    PMID: 39647778 DOI: 10.1016/j.jshs.2024.101014
    BACKGROUND: Muscular strength is a powerful marker of current health status and robust predictor of age-related disease and disability. Handgrip strength (HGS) using isometric dynamometry is a convenient, feasible, and widely used method of assessing muscular strength among people of all ages. While adult HGS norms have been published for many countries, no study has yet synthesized available data to produce international norms. The objective of this study was to generate international sex- and age-specific norms for absolute and body size-normalized HGS across the adult lifespan.

    METHODS: Systematic searches were conducted in 6 databases/web search engines (MEDLINE, SPORTDiscus, Embase, Web of Science, CINAHL, and Google Scholar) up to December 1, 2023. We included full-text peer-reviewed observational studies that reported normative HGS data for adults aged ≥20 years by sex and age. Pseudo data were generated using Monte Carlo simulation following harmonization for methodological variation. Population-weighted Generalized Additive Models for Location, Scale, and Shape were used to develop sex- and age-specific norms for absolute HGS (kg) and HGS normalized by height (Ht, m) squared (i.e., HGS/Ht2 in kg/m2). Norms were tabulated as percentile values (5th to 95th) and visualized as smoothed percentile curves.

    RESULTS: We included data from 100 unique observational studies representing 2,405,863 adults (51.9% female) aged 20 to 100+ years from 69 countries and regions tested from the year 2000 onward. On average, absolute and normalized HGS values negligibly improved throughout early adulthood, peaked from age 30-39 years (at 49.7 kg (males) and 29.7 kg (females) for absolute HGS or 16.3 kg/m2 (males) and 11.3 kg/m2 (females) for HGS/Ht2), and declined afterwards. The age-related decline in HGS accelerated from middle to late adulthood and was slightly larger for males than for females during middle adulthood.

    CONCLUSION: This study provides the world's largest and most geographically comprehensive international norms for adult HGS by sex and age. These norms have utility for global peer-comparisons, health screening, and surveillance.

  19. Yik ALP, Barodawala FS
    Rom J Ophthalmol, 2024;68(4):398-403.
    PMID: 39936048 DOI: 10.22336/rjo.2024.72
    OBJECTIVES: To evaluate the agreement between Spectral-Domain Optical Coherence Tomography (AS-OCT) and Oculus Keratograph® 5M in measuring tear meniscus height (TMH) and to assess the impact of contact lens wear on these measurements. Materials and methods: TMH was measured in 54 healthy eyes using AS-OCT (3D OCT-1 Maestro, Topcon, Tokyo, Japan) and Oculus Keratograph® 5M (OCULUS Optikgeräte, Wetzlar, Germany), with and without contact lens wear. Bland-Altman analysis was used to assess agreement between the two devices. Delefilcon A, water-gradient, daily disposable contact lenses were used, and measurements were carried out after a 20-minute adaptation period.

    RESULTS: The means of TMH without the contact lenses were 0.21 ± 0.06 mm and 0.20 ± 0.05 mm obtained from AS-OCT and Oculus K5M, respectively, and these measurements were not statistically significant (t (53) = 0.99, p = 0.33). No significant differences were observed in TMH compared to contact lenses (t (53) = 1.52, p = 0.13). Agreement between measurements obtained by both the instruments was assessed using Bland-Altman analysis. The limits of agreement were within clinically acceptable ranges (0.10 mm - 0.15 mm), with no evidence of significant bias (t = -0.32, r = 0.22). The results obtained with contact lenses were also not statistically significant (t (53) = 1.52, p < 0.05).

    DISCUSSION: The present study compared tear meniscus height (TMH) measurements obtained from AS-OCT and Oculus K5M in subjects with and without contact lens wear. Both instruments showed good agreement, with AS-OCT consistently measuring slightly higher TMH values than Oculus K5M. The mean TMH values were similar to those of previous studies, indicating normal tear film in the subjects. Contact lens wear was found to reduce TMH slightly, but it returned to baseline after a short adaptation period. The Bland-Altman analysis confirmed good agreement between the two instruments, with most data points falling within the limits of agreement. These findings suggest that AS-OCT and Oculus K5M can be reliable tools for measuring TMH and can be used interchangeably for clinical practice.

    CONCLUSION: AS-OCT and Oculus Keratograph® 5M showed comparable results in measuring TMH, suggesting potential interchangeability in clinical practice. Further validation in broader clinical settings and diverse subject groups may be warranted.

    MeSH terms: Adult; Contact Lenses; Female; Humans; Male; Middle Aged; Reproducibility of Results; Young Adult; Healthy Volunteers
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