Effect of type and concentration of cryoprotectant on the motility, viability, and fertility of climbing perch Anabas testudineus Bloch, 1792 (Pisces: Anabantidae) sperm

Maulida S 1 , Eriani K 2 , Fadli N 3 , Kocabaş FK 4 , Siti-Azizah MN 5 , Wilkes M 6 Show all authors , Muchlisin ZA 7

Affiliations 

  • 1 Doctoral Program of Mathematics and Applied Sciences, Postgraduate School of Universitas Syiah Kuala, Banda Aceh, 23111, Indonesia; Department of Aquaculture, Faculty of Marine and Fisheries, Universitas Syiah Kuala, Banda Aceh, 23111, Indonesia
  • 2 Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Syiah Kuala, Banda Aceh, 23111, Indonesia
  • 3 Department of Aquaculture, Faculty of Marine and Fisheries, Universitas Syiah Kuala, Banda Aceh, 23111, Indonesia
  • 4 Faculty of Fisheries, Munzur University, Turkey
  • 5 Institute of Marine Biotechnology, Universiti Malaysia Terengganu, Kuala Terengganu, Malaysia
  • 6 University of Essex, Colchester, CO4 3SQ, United Kingdom
  • 7 Doctoral Program of Mathematics and Applied Sciences, Postgraduate School of Universitas Syiah Kuala, Banda Aceh, 23111, Indonesia; Department of Aquaculture, Faculty of Marine and Fisheries, Universitas Syiah Kuala, Banda Aceh, 23111, Indonesia. Electronic address: muchlisinza@usk.ac.id
Theriogenology, 2023 Apr 15;201:24-29.
PMID: 36822040 DOI: 10.1016/j.theriogenology.2023.02.014

Abstract

The climbing perch, Anabas testudineus is a freshwater fish that has economic value in Indonesia. It is cultured in the country, but the breeding technology, specifically sperm storage, is not well developed. Sperm cryopreservation is one of the preservation methods that need to be developed to support fish breeding technology. The type of cryoprotectants and its concentration are species-dependent and determines the success of this approach. Therefore, this study is aimed at determining the optimal type and concentration of cryoprotectant for sperm cryopreservation of A. testudineus. Four separate study series were performed, each of which evaluated one type of cryoprotectant at five concentration levels. The cryoprotectants used were DMSO, methanol, glycerol, and ethanol, and the tested concentrations were 0%, 5%, 10%, 15%, and 20%, which were combined with 5% egg yolks. Each treatment was conducted with three replications. The results showed that the type of cryoprotectant and its concentration significantly affected sperm motility, viability, and fertility of climbing perch (P 

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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