Affiliations 

  • 1 Colin Ratledge Centre for Microbial Lipids, School of Agriculture Engineering and Food Science, Shandong University of Technology, Zibo, China; Interdisciplinary Graduate Programs in Bioscience, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand
  • 2 Institute of Nutrition, Mahidol University, Nakhon Pathom, Thailand
  • 3 Interdisciplinary Graduate Programs in Bioscience, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand
  • 4 Colin Ratledge Centre for Microbial Lipids, School of Agriculture Engineering and Food Science, Shandong University of Technology, Zibo, China; Department of Food Sciences, Faculty of Science and Technology, University Kebangsaan Malaysia, UKM, Bangi, Malaysia
  • 5 Interdisciplinary Graduate Programs in Bioscience, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand; Department of Zoology, Faculty of Science, Kasetsart University, Bangkok, Thailand
  • 6 Department of Zoology, Faculty of Science, Kasetsart University, Bangkok, Thailand; Genetic Engineering and Bioinformatic Program, Graduate School, Kasetsart University, Bangkok, Thailand
  • 7 Department of Biological Science and Biotechnology, Faculty of Science and Technology, National University of Malaysia, Bangi, Malaysia. Electronic address: aidilah@ukm.edu.my
  • 8 Department of Zoology, Faculty of Science, Kasetsart University, Bangkok, Thailand; Omics Center for Agriculture, Bioresources, Food, and Health, Kasetsart University (OmiKU), Bangkok, Thailand. Electronic address: wanwipa.v@ku.ac.th
  • 9 Colin Ratledge Centre for Microbial Lipids, School of Agriculture Engineering and Food Science, Shandong University of Technology, Zibo, China. Electronic address: ysong@sdut.edu.cn
PMID: 36907245 DOI: 10.1016/j.bbalip.2023.159306

Abstract

Aurantiochytrium sp. SW1, a marine thraustochytrid, has been regarded as a potential candidate as a docosahexaenoic acid (DHA) producer. Even though the genomics of Aurantiochytrium sp. are available, the metabolic responses at a systems level are largely unknown. Therefore, this study aimed to investigate the global metabolic responses to DHA production in Aurantiochytrium sp. through transcriptome and genome-scale network-driven analysis. Of a total of 13,505 genes, 2527 differentially expressed genes (DEGs) were identified in Aurantiochytrium sp., unravelling the transcriptional regulations behinds lipid and DHA accumulation. The highest number of DEG were found for pairwise comparison between growth phase and lipid accumulating phase where a total of 1435 genes were down-regulated with 869 genes being up-regulated. These uncovered several metabolic pathways that contributing in DHA and lipid accumulation including amino acid and acetate metabolism which involve in the generation of crucial precursors. Upon applying network-driven analysis, hydrogen sulphide was found as potential reporter metabolite that could be associated with the genes related to acetyl-CoA synthesis for DHA production. Our findings suggest that the transcriptional regulation of these pathways is a ubiquitous feature in response to specific cultivation phases during DHA overproduction in Aurantiochytrium sp. SW1.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.