Antidepressant evaluation of Andrographis paniculata Nees extract and andrographolide in chronic unpredictable stress zebrafish model

Aldurrah Z 1 , Mohd Kauli FS 2 , Abdul Rahim N 2 , Zainal Z 3 , Afzan A 4 , Al Zarzour RH 5 Show all authors , Muhamad Salhimi S 2 , Che Zain MS 6 , Zakaria F 7

Affiliations 

  • 1 Discipline of Pharmaceutical Chemistry, School of Pharmaceutical Sciences, Universiti Sains Malaysia, 11800 USM, Penang, Malaysia. Electronic address: https://twitter.com/zenab_aldurrah
  • 2 Discipline of Pharmaceutical Chemistry, School of Pharmaceutical Sciences, Universiti Sains Malaysia, 11800 USM, Penang, Malaysia
  • 3 Natural Medicines and Products Research Laboratory (NaturMeds), Institute of Bioscience, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia
  • 4 Phytochemistry Unit, Herbal Medicine Research Institute, Institute for Medical Research, National Institutes of Health, 40170 Shah Alam, Malaysia
  • 5 Discipline of Pharmacology, School of Pharmaceutical Sciences, Universiti Sains Malaysia, 11800 USM, Penang, Malaysia; Department of Pharmacology, Faculty of Pharmacy, Arab International University (AIU), Syria
  • 6 Bioresource Technology Division, School of Industrial Technology, Universiti Sains Malaysia, 11800 Gelugor, Penang, Malaysia
  • 7 Discipline of Pharmaceutical Chemistry, School of Pharmaceutical Sciences, Universiti Sains Malaysia, 11800 USM, Penang, Malaysia. Electronic address: fauziahanimz@usm.my
Comp Biochem Physiol C Toxicol Pharmacol, 2023 Sep;271:109678.
PMID: 37301417 DOI: 10.1016/j.cbpc.2023.109678

Abstract

Andrographis paniculata (A. paniculata) showed an anti-depressive effect in rodent models. Zebrafish has recently emerged as a worthy complementary translational model for antidepressant drug discovery study. This study investigates the anti-depressive effect of A. paniculata extract and andrographolide in the chronic unpredictable stress (CUS)- zebrafish model. Four groups of zebrafish (n = 10/group), i.e. control, CUS (stressed, untreated), CUS + A. paniculata (100 mg/L) and CUS + fluoxetine (0.01 mg/L) were assessed in open-field and social interaction tests, 24 h after treatment. After extract screening, behavioural and cortisol analysis of andrographolide (5, 25 and 50 mg/kg, i.p.) and fluoxetine (10 mg/kg, i.p.) were evaluated. Before the behavioural study, acute toxicity and characterization of A. paniculata extract using UHPLC-ESI-MS/MS were performed. A significant reduction in freezing duration was found in A. paniculata- (t-test, p = 0.0234) and fluoxetine-treated groups (t-test, p 

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