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Isolation of Spirosoma foliorum sp. nov. from the fallen leaf of Acer palmatum by a novel cultivation technique

Han HL 1 , Nurcahyanto DA 2 , Muhammad N 3 , Lee YJ 3 , Nguyen TTH 3 , Kim SG 4 Show all authors , Chan SS 5 , Khoo KS 6 , Chew KW 7 , Show PL 8 , Tran TNT 9 , Nguyen TDP 10 , Chiu CY 11

Affiliations 

  • 1 The University of Danang, University of Science and Technology, 54 Nguyen Luong Bang St., Danang, 550 000, Viet Nam
  • 2 Research Center for Biosystematics and Evolution, Research Organization for Life Sciences and Environment, National Research and Innovation Agency (BRIN), Cibinong 16911, West Java, Indonesia
  • 3 Biological Resource Center/Korean Collection for Type Cultures (KCTC), Korea Research Institute of Bioscience and Biotechnology, 181 Ipsingil, Jeongeup, 56212, Jeonbuk, Korea
  • 4 Biological Resource Center/Korean Collection for Type Cultures (KCTC), Korea Research Institute of Bioscience and Biotechnology, 181 Ipsingil, Jeongeup, 56212, Jeonbuk, Korea. sgkim@kribb.re.kr
  • 5 Institut Sains Biologi, Fakulti Sains, Universiti Malaya, Kuala Lumpur, Malaysia
  • 6 Department of Chemical Engineering and Materials Science, Yuan Ze University, Taoyuan, Taiwan
  • 7 School of Chemistry, Chemical Engineering and Biotechnology, Nanyang Technological University, 62 Nanyang Drive, Singapore, 637459, Singapore
  • 8 Department of Chemical Engineering, Khalifa University, Shakhbout Bin Sultan St - Zone 1, Abu Dhabi, United Arab Emirates. pauloke.show@ku.ac.ae
  • 9 The University of Da Nang, University of Technology and Education, Da Nang City, 550000, Viet Nam
  • 10 The University of Da Nang, University of Technology and Education, Da Nang City, 550000, Viet Nam. ntdphuong@ute.udn.vn
  • 11 Biochemical Engineering Research Center, Ming Chi University of Technology, New Taipei City, 24301, Taiwan. chenyaw.chiu@gmail.com
Sci Rep, 2023 Sep 06;13(1):14684.
PMID: 37673882 DOI: 10.1038/s41598-023-35108-5

Abstract

In the effort of isolating novel microbial species, the strain PL0132T was isolated from a fallen leaf under fresh water at a stream, which glided when grown on a tap water medium (without nutrients). The strain was determined to be Gram-negative, strictly aerobic, and rod-shaped, which grew optimally at 25 °C, pH 6-7, and the strain tolerates 1% (w/v) NaCl concentration. The complete genome of strain PL0132T comprises one contig with a sequencing depth of 76×, consisting of 8,853,064 base pairs and the genomic DNA G + C content was 46.7% (genome). 16S rRNA gene sequence analysis revealed that strain PL0132T represents a member of the phylum Bacteroidetes and is affiliated with the genus Spirosoma. Based on genomic, phenotypic, and chemotaxonomic characteristics, the strain PL0132T represents a novel species of the genus Spirosoma, for which the name Spirosoma foliorum sp. nov. is proposed (= KCTC 72228 T = InaCC B1447T).

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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