Affiliations 

  • 1 Jiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, Lianyungang 222005, China; Marine Resource Development institute of Jiangsu (Lianyungang), Jiangsu Lianyungang, 222005, China
  • 2 Jiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, Lianyungang 222005, China; Marine Resource Development institute of Jiangsu (Lianyungang), Jiangsu Lianyungang, 222005, China; Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Lianyungang, Jiangsu 222005, China; The Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm, Nanjing, Jiangsu 210014, China
  • 3 Institute of Climate Adaptation and Marine Biotechnology, Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia. Electronic address: azramn@umt.edu.my
  • 4 Department of Freshwater Biology and Fisheries, University of Sindh, Jamshoro 76080, Pakistan. Electronic address: wazir.baloch@usindh.edu.pk
  • 5 Jiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, Lianyungang 222005, China; Marine Resource Development institute of Jiangsu (Lianyungang), Jiangsu Lianyungang, 222005, China; Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Lianyungang, Jiangsu 222005, China; The Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm, Nanjing, Jiangsu 210014, China. Electronic address: huanmr@163.com
Genomics, 2023 Nov;115(6):110746.
PMID: 37977333 DOI: 10.1016/j.ygeno.2023.110746

Abstract

To study the mechanism of the biomolecular response in Exopalaemon carinicauda to starvation stress, we subjected muscle tissue RNA samples from four stress points, including 0 d(control group), 10 d, 20 d, and 30 d, to starvation stress on white ridgetail prawn with a body weight of 1.41 + 0.42 g, aquaculture water temperature of 23-25 °C, salinity of 26, dissolved oxygen ≥5 mg/L, and pH 8-8.5, Then performed de novo transcriptome assembly and gene expression analysis using BGISEQ-500 with a tag-based digital gene expression (DGE) system. By de novo assembling at the four times, we obtained 28,167, 21,115, 24,497, and 27,080 reads, respectively. The results showed that the stress at 10 d led to no significant difference in the expressed genes, while the stress at 20 d and 30 d showed a significant increase (or decrease) in the expression of 97 (276) and 143 (410) genes, respectively, which were involved in 8 different metabolic pathways. In addition, we detected 2647 unigene transcription factors. Eleven upregulated and sixteen downregulated genes from the different starvation stress groups were choose to verify the reliability of the transcriptome data, and the results showed that the expression trends of these genes were consistent with the results shown by the transcriptome. The analysis of the experimental data and our discussion of the response mechanism of white ridgetail prawn under starvation stress provides a foundation for further screening of the key genes of starvation stress and may help to elucidate their functions.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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