Affiliations 

  • 1 College of Tea and Food Science, Wuyi University, Wuyishan 354300, PR China; Food Technology Division, School of Industrial Technology, Universiti Sains Malaysia USM, Penang 11800, Malaysia
  • 2 College of Tea and Food Science, Wuyi University, Wuyishan 354300, PR China
  • 3 College of Tea and Food Science, Wuyi University, Wuyishan 354300, PR China. Electronic address: yanhuangfst@wuyiu.edu.cn
  • 4 Food Technology Division, School of Industrial Technology, Universiti Sains Malaysia USM, Penang 11800, Malaysia; Renewable Biomass Transformation Cluster, School of Industrial Technology, Universiti Sains Malaysia USM, Penang 11800, Malaysia. Electronic address: thuanchew@usm.my
  • 5 Department of Food Industry, Faculty of Bioresources and Food Industry, Universiti Sultan Zainal Abidin, Besut, Terengganu 22200, Malaysia
J Chromatogr A, 2024 Jan 25;1715:464621.
PMID: 38198876 DOI: 10.1016/j.chroma.2023.464621

Abstract

White tea contains the highest flavonoids compared to other teas. While there have been numerous studies on the components of different tea varieties, research explicitly focusing on the flavonoid content of white tea remains scarce, making the need for a good flavonoid purification process for white tea even more important. This study compared the adsorption and desorption performance of five types of macroporous resins: D101, HP20, HPD500, DM301, and AB-8. Among the tested resins, AB-8 was selected based on its best adsorption and desorption performance to investigate the static adsorption kinetics and dynamic adsorption-desorption purification of white tea flavonoids. The optimal purification process was determined: adsorption temperature 25 °C, crude tea flavonoid extract pH 3, ethanol concentration 80 %, sample loading flow rate and eluent flow rate 1.5 BV/min, and eluent dosage 40 BV. The results indicated that the adsorption process followed pseudo-second-order kinetics. Under the above purification conditions, the purity of the total flavonoids in the purified white tea flavonoid increased from approximately 17.69 to 46.23 %, achieving a 2.61-fold improvement, indicating good purification results. The purified white tea flavonoid can be further used for nutraceutical and pharmaceutical applications.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.