Affiliations 

  • 1 Centro de Investigaciones Odontologicas, School of Dentistry, Pontificia Universidad Javeriana, Bogota, Colombia
  • 2 Postgraduate Endodontics Department, School of Dentistry, Universidad Cooperativa de Colombia, Pasto, Colombia
  • 3 School of Dentistry, Mahsa University, Kuala Lumpur, Malaysia
  • 4 Instituto Venezolano de Investigaciones Científicas, Caracas, Venezuela
  • 5 Engineer School, Universidad de La Sabana, Chía, Colombia
  • 6 Endodontics Department, School of Dentistry, Universidad de San Carlos de Guatemala, Guatemala City, Guatemala
Int Endod J, 2024 May;57(5):576-585.
PMID: 38294105 DOI: 10.1111/iej.14035

Abstract

AIM: The purpose of this study was to quantify the effect of five different root canal preparation instruments on Substance P (SP), Calcitonin gene-related peptide (CGRP) and their receptors expression in healthy human periodontal ligament.

METHODOLOGY: STROBE guidelines were used to design a study using 60 periodontal ligament samples obtained from healthy lower premolars where extraction was indicated for orthodontic reasons. Prior to extraction 40 of these premolars were equally divided into four groups and root canals were prepared using different systems: Mtwo, Reciproc Blue, HyFlex EDM and Plex-V. Ten premolars were prepared with hand files and served as a positive control group. The remaining 10 premolars where extracted without treatment and served as a negative control group. All periodontal ligament samples were processed to measure the expression of SP, CGRP and their receptors by radioimmunoassay. Kruskal-Wallis and Duncan tests were performed to determine statistically significant differences between the groups for each variable.

RESULTS: Greater expression of all the peptides measured were found in the hand-file preparation group, followed by the Reciproc Blue, Mtwo, HyFlex EDM and Plex-V groups. The lower SP, CGRP and their receptors values were for the intact teeth control group. Kruskal-Wallis test showed statistically significant differences amongst groups (p 

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.