Affiliations 

  • 1 Centre for Neuroscience Research (NeuRon), Faculty of Medicine, Universiti Teknologi MARA 47000, Sungai Buloh, Selangor, Malaysia; Laboratory Animal Care Unit (LACU), Faculty of Medicine, Universiti Teknologi MARA 47000, Sungai Buloh, Selangor, Malaysia; Institute for Molecular Medicine and Biotechnology (IMMB) Faculty of Medicine, Universiti Teknologi MARA 47000, Sungai Buloh, Selangor, Malaysia
  • 2 Institute for Molecular Medicine and Biotechnology (IMMB) Faculty of Medicine, Universiti Teknologi MARA 47000, Sungai Buloh, Selangor, Malaysia
  • 3 Faculty of Dentistry, Universiti Teknologi MARA 47000, Sungai Buloh, Selangor, Malaysia; Neuroscience Research Group (NRG), Faculty of Medicine, Universiti Teknologi MARA, 47000, Sungai Buloh, Selangor, Malaysia
  • 4 Centre for Neuroscience Research (NeuRon), Faculty of Medicine, Universiti Teknologi MARA 47000, Sungai Buloh, Selangor, Malaysia; Neuroscience Research Group (NRG), Faculty of Medicine, Universiti Teknologi MARA, 47000, Sungai Buloh, Selangor, Malaysia.. Electronic address: rosfaiizah@uitm.edu.my
Physiol Behav, 2024 Jun 01;280:114546.
PMID: 38583549 DOI: 10.1016/j.physbeh.2024.114546

Abstract

Maternal bisphenol A (BPA) exposure has been reported to cause learning and memory deficits in born offspring. However, little is known that this impairment is potentially caused by epigenetic modulation on the development of NMDA receptor subunits. This study investigates the effect of prenatal BPA exposure on the hippocampal miR-19a and miR-539, which are responsible for regulating NMDA receptor subunits as well as learning and memory functions. Pregnant Sprague Dawley rats were orally administered with 5 mg/kg/day of BPA from pregnancy day 1 (PD1) until gestation day 21 (GD21), while control mothers received no BPA. The mothers were observed daily until GD21 for either a cesarean section or spontaneous delivery. The male offspring were sacrificed when reaching GD21 (fetus), postnatal days 7, 14, 21 (PND7, 14, 21) and adolescent age 35 (AD35) where their hippocampi were dissected from the brain. The expression of targeted miR-19a, miR-539, GRIN2A, and GRIN2B were determined by qRT-PCR while the level of GluN2A and GluN2B were estimated by western blot. At AD35, the rats were assessed with neurobehavioral tests to evaluate their learning and memory function. The findings showed that prenatal BPA exposure at 5 mg/kg/day significantly reduces the expression of miR-19a, miR-539, GRIN2A, and GRIN2B genes in the male rat hippocampus at all ages. The level of GluN2A and GluN2B proteins is also significantly reduced when reaching adolescent age. Consequently, the rats showed spatial and fear memory impairments when reaching AD35. In conclusion, prenatal BPA exposure disrupts the role of miR-19a and miR-539 in regulating the NMDA receptor subunit in the hippocampus which may be one of the causes of memory and learning impairment in adolescent rats.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.