Effect of trans-resveratrol on dexamethasone-induced changes in the expression of MMPs by human trabecular meshwork cells: Involvement of adenosine A1 receptors and NFkB

Mohd Nasir NA 1 , Agarwal R 2 , Krasilnikova A 3 , Sheikh Abdul Kadir SH 4 , Iezhitsa I 5

Affiliations 

  • 1 Center for Neuroscience Research (NeuRon), Faculty of Medicine, Universiti Teknologi MARA (UiTM), Sungai Buloh Campus, Selangor, Malaysia; Institute of Medical Molecular Biotechnology (IMMB), Universiti Teknologi MARA (UiTM), Sungai Buloh Campus, Selangor, Malaysia
  • 2 School of Medicine, International Medical University, Bukit Jalil, 57000, Kuala Lumpur, Malaysia. Electronic address: renuag02@gmail.com
  • 3 Faculty of Medicine, Universiti Teknologi MARA (UiTM), Sungai Buloh Campus, Selangor, Malaysia; Volgograd State Medical University, Department of Clinical Pharmacology & Intensive Care, Volgograd, Russia
  • 4 Faculty of Medicine, Universiti Teknologi MARA (UiTM), Sungai Buloh Campus, Selangor, Malaysia; Institute of Medical Molecular Biotechnology (IMMB), Universiti Teknologi MARA (UiTM), Sungai Buloh Campus, Selangor, Malaysia
  • 5 School of Medicine, International Medical University, Bukit Jalil, 57000, Kuala Lumpur, Malaysia
Eur J Pharmacol, 2020 Nov 15;887:173431.
PMID: 32758568 DOI: 10.1016/j.ejphar.2020.173431

Abstract

Intraocular pressure (IOP) lowering in glaucomatous eyes is currently achieved mainly by improved aqueous outflow via alternate drainage pathways. However, the focus is now shifting to trabecular meshwork (TM), the site or major pathological changes including increased extracellular matrix (ECM) deposition and reduced matrix metalloproteinases (MMPs) secretion by TM cells. Trans-resveratrol was previously shown to lower IOP and reduce ECM deposition; however, the mechanisms of action remain unclear. Therefore, we determined the effect of trans-resveratrol on MMP-2 and -9 expression by human TM cells (HTMCs) in the presence of dexamethasone and whether it also affects adenosine A1 receptors (A1AR) expression and nuclear factor kappa B (NFkB) activation. We observed that trans-resveratrol, 12.5 μM, increased MMP-2 and -9 protein expression by HTMCs despite exposure to dexamethasone (1.89- and 1.53-fold, respectively; P 

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