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Rapid immunoglobulin M-based dengue diagnostic test using surface plasmon resonance biosensor

Jahanshahi P 1 , Zalnezhad E 2 , Sekaran SD 3 , Adikan FR 1

Affiliations 

  • 1 Photonics Research Group, Department of Electrical Engineering, Faculty of Engineering, University of Malaya, 50603 Kuala Lumpur, Malaysia
  • 2 Center of Advanced Manufacturing and Material Processing, Department of Engineering Design and Manufacture, Faculty of Engineering, University of Malaya, 50603 Kuala Lumpur, Malaysia
  • 3 Department of Medical Microbiology, Department of Microbiology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia
Sci Rep, 2014 Jan 24;4:3851.
PMID: 24458089 DOI: 10.1038/srep03851

Abstract

Surface plasmon resonance (SPR) is a medical diagnosis technique with high sensitivity and specificity. In this research, a new method based on SPR is proposed for rapid, 10-minute detection of the anti-dengue virus in human serum samples. This novel technique, known as rapid immunoglobulin M (IgM)-based dengue diagnostic test, can be utilized quickly and easily at the point of care. Four dengue virus serotypes were used as ligands on a biochip. According to the results, a serum volume of only 1 μl from a dengue patient (as a minimized volume) is required to indicate SPR angle variation to determine the ratio of each dengue serotype in samples with 83-93% sensitivity and 100% specificity.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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