Fulltext

Antibacterial potency of mid-polar extracts obtained from Malaysian plant Parkia speciosa against human pathogenic bacteria

Goh SM 1 , Dassanayake MK 2 , Foan CC 1 , Wiart C 3 , Symonds R 4 , Khoo TJ 5 Show all authors , Chong CH 6 , Elfar OA 1

Affiliations 

  • 1 School of Pharmacy, Faculty of Science and Engineering, University of Nottingham Malaysia, Jalan Broga, Semenyih, 43500, Malaysia
  • 2 School of Pharmacy, Faculty of Science and Engineering, University of Nottingham Malaysia, Jalan Broga, Semenyih, 43500, Malaysia. Electronic address: rapter100000@yahoo.com
  • 3 Institute of Tropical Biology and Conservation, University Malaysia Sabah, 88400, Jalan Sulaman, Kota Kinabalu, Sabah, Malaysia
  • 4 School of Biological and Environmental Sciences, Faculty of Science, Liverpool John Moores University, James Parsons Building, Byrom St, Liverpool, L3 3AF, United Kingdom
  • 5 School of Pharmacy, Faculty of Science and Engineering, University of Nottingham Malaysia, Jalan Broga, Semenyih, 43500, Malaysia. Electronic address: Tengjin.khoo@nottingham.edu.my
  • 6 Department of Chemical and Environmental Engineering, Faculty of Science and Engineering, University of Nottingham Malaysia, Jalan Broga, Semenyih, 43500, Malaysia
Microb Pathog, 2025 Jan;198:107134.
PMID: 39566830 DOI: 10.1016/j.micpath.2024.107134

Abstract

BACKGROUND AND OBJECTIVES: Plants contain a wide variety of bioactive compounds, which have attracted the interest of researchers in finding novel sources of natural medicine. In the following paper, we aim to evaluate the antibacterial potential of extract fractions associated with Parkia speciosa pods and beans against human pathogenic bacteria.

METHODS: Antimicrobial activity was determined with disc diffusion and broth microdilution assays against eight skin colonising microorganisms including Staphylococcus aureus, Staphylococcus epidermidis, Salmonella enterica, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumonia followed by further fractionation of the pods ethyl acetate fraction by column chromatography along with preparative thin-layer chromatography. Quantification of bacterial death mechanism was elucidated by the measurement of hole size in cell wall that has been induced by extract constituents via field-emission scanning electron microscopy (FESEM).

RESULTS: Four fractions showed significant antimicrobial activity against the six microorganisms tested (p 

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

MeSH terms
Similar publications