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Detection of Vibrio cholerae 01 from aquatic environment in Sarawak

Norazah A 1 , Zainuldin MT 2 , Kamel AGM 3 , Kamaliah MN 4 , Taha AM 5

Affiliations 

  • 1 A Norazah, MD. Bacteriology Division, Institute for Medical Research, Jalan Pahang, 50588 Kuala Lumpur
  • 2 M T Zainuldin, BSc. Bacteriology Division, Institute for Medical Research, Jalan Pahang, 50588 Kuala Lumpur
  • 3 A G M Kamel, MD. Department of Biomedical Sciences, Faculty of Allied Health Sciences National University of Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur
  • 4 M N Kamaliah, MPH. Pejabat Pengarah Perkhidmatan & Kesihatan, Jalan Tun Abang Haji Openg, 93590 Kuching
  • 5 A Mohamad Taha, DPH. Pejabat Kawalan Penyakit Berjangkit, Jabatan Kesihatan Awam, Kementerian Kesihatan Malaysia, Jalan Dungun, Bukit Damansara, 50490 Kuala Lumpur
Med J Malaysia, 2001 Mar;56(1):4-9.
PMID: 11503295

Abstract

The detection of Vibrio cholerae 01 from the aquatic environment of Daro and Bintulu in Sarawak was carried out following an outbreak of cholera. Conventional culture methods and detection of ctx gene by polymerase chain reaction technique were carried out on 80 water samples. Only one sample was positive by culture methods while 8 were positive by PCR. DNA finger printing by pulsed-field gel electrophoresis showed that the clinical isolates in Daro and Bintulu were genetically identical while the environmental isolate was closely related. Recovery of Vibrio cholerae by culture method is poor and newer methods of detection should be developed.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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