Affiliations 

  • 1 Department of Pharmaceutical Sciences, University of Shizuoka , Shizuoka 422-8526, Japan
  • 2 School of Biosciences, The University of Nottingham Malaysia Campus , Semenyih, Selangor Darul Ehsan 43500, Malaysia
Org. Lett., 2015 Oct 16;17(20):4992-5.
PMID: 26414728 DOI: 10.1021/acs.orglett.5b02435

Abstract

Successful activation of the pyranonigrin biosynthetic gene cluster and gene knockout in Aspergillus niger plus in vivo and in vitro assays led to isolation of six new products, including a spiro cyclobutane-containing dimeric compound, which served as the basis for the proposed comprehensive pyranonigrin biosynthetic pathway. Two redox enzymes are key to forming the characteristic fused γ-pyrone core, and a protease homologue performs the exo-methylene formation.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.