Identification of Optimal Reference Genes for Normalization of RT-qPCR Data in Cancerous and Non-Cancerous Tissues of Human Uterine Cervix

Tan SC 1 , Ismail MP 2 , Duski DR 3 , Othman NH 4 , Bhavaraju VM 5 , Ankathil R 1

Affiliations 

  • 1 a Human Genome Centre, School of Medical Sciences, Health Campus , Universiti Sains Malaysia , Kelantan , Malaysia
  • 2 b Department of Obstetrics and Gynecology , School of Medical Sciences, Health Campus, Universiti Sains Malaysia , Kelantan , Malaysia
  • 3 c Department of Obstetrics and Gynecology , Hospital Sultan Ismail , Johor , Malaysia
  • 4 d Department of Pathology , School of Medical Sciences, Health Campus, Universiti Sains Malaysia , Kelantan , Malaysia
  • 5 e Department of Nuclear Medicine , Radiotherapy and Oncology, School of Medical Sciences, Health Campus, Universiti Sains Malaysia , Kelantan , Malaysia
Cancer Invest, 2017 Mar 16;35(3):163-173.
PMID: 28301252 DOI: 10.1080/07357907.2017.1278767

Abstract

This study aimed to identify the most stably expressed reference genes from a panel of 32 candidate genes for normalization of reverse transcription-quantitative real-time polymerase chain reaction data in cancerous and non-cancerous tissues of human uterine cervix. Overall, PUM1, YWHAZ, and RPLP0 were identified as the most stably expressed genes in paired cancerous and non-cancerous tissues. The results were further stratified by the state of malignancy of the tissues, histopathological type of the cancer, and the human papillomavirus-type.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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